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Phil Trans R Soc Lond [Biol]. 1\u2013214 p."},{"key":"ref13","first-page":"279","article-title":"The regulation of glycolysis and the pentose phosphate pathway","volume":"2","author":"JF Turner","year":"1980","journal-title":"New York: Academic Press"},{"key":"ref14","first-page":"249","article-title":"Cellular biochemistry of glucose-6-phosphate and 6-phosphogluconate dehydrogenase activities","volume":"4","author":"J Chayan","year":"1986","journal-title":"Cell BiochemFunct"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1002\/cbf.290040403","article-title":"Physiological functions of the pentose phosphate pathway","volume":"4","author":"T Wood","year":"1986","journal-title":"Cell Biochem Funct"},{"key":"ref16","unstructured":"Colowick S (1973) The hexokinases. In: Boyer PB (ed) The enzymes, vol. 9. Academic, New York, 1\u201348."},{"key":"ref17","first-page":"745","article-title":"Evolutionary aspects of lower deuterostomian hexokinases","volume":"70B","author":"Y Mochizuki","year":"1981","journal-title":"Comp Biochem Physiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0020-1790(78)90040-9","article-title":"Tissue distribution, purification, and properties ofmultiple forms of hexokinase in the silkworm, Bombyx mori","volume":"8","author":"HA Yanagawa","year":"1978","journal-title":"Insect Biochem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"24736","DOI":"10.1016\/S0021-9258(17)31453-9","article-title":"The 50-kDa glucose 6-phosphate-sensitive hexokinase of Schistosoma mansoni","volume":"269","author":"AG Tielens","year":"1994","journal-title":"J Biol Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/BFb0049776","article-title":"Hexokinases","volume":"126","author":"JE Wilson","year":"1995","journal-title":"Rev Physiol Biochem Pharmacol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1073\/pnas.54.4.1218","article-title":"Multiple forms of hexokinase in the rat: tissue distribution, age dependency, and properties","volume":"54","author":"RT KatzenHM, Schimke","year":"1965","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1021\/bi00854a014","article-title":"Characterization of isoenzymes of adenosine triphosphate: D-hexose-6-phosphotransferase from rat liver","volume":"6","author":"C Gonzalez","year":"1967","journal-title":"Biochemistry"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/0003-9861(89)90451-7","article-title":"Isolation and characterization of the discrete N- and C-terminal halves of rat brain hexokinase: retention of full catalytic activity in the isolated C-terminal half","volume":"274","author":"TK White","year":"1989","journal-title":"Arch Biochem Biophys"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1074\/jbc.271.4.1849","article-title":"Functional organization of mammalian hexokinase II. 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