{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:30:08Z","timestamp":1789893008382,"version":"4.0.1"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T00:00:00Z","timestamp":1411689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0108606","type":"journal-article","created":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T18:46:17Z","timestamp":1411757177000},"page":"e108606","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Early Transcriptional Responses of Bovine Chorioallantoic Membrane Explants to Wild Type, \u0394virB2 or \u0394btpB Brucella abortus Infection"],"prefix":"10.1371","volume":"9","author":[{"given":"Juliana P. S.","family":"Mol","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica A.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex F.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao-Hui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u00e9 M.","family":"Tsolis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane A.","family":"Paix\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato L.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,9,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","article-title":"Brucellosis: a worldwide zoonosis","volume":"4","author":"ML Boschiroli","year":"2001","journal-title":"Curr Opin Microbiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1111\/j.1365-2672.2005.02622.x","article-title":"Brucellosis - new aspects of an old disease","volume":"98","author":"SJ Cutler","year":"2005","journal-title":"J Appl Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1590\/S0100-736X2013000600012","article-title":"Economic losses due to bovine brucellosis in Brazil","volume":"33","author":"RL Santos","year":"2013","journal-title":"Pesq Vet Bras"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0147-9571(93)90001-L","article-title":"Pathogenesis of abortion of bovine brucellosis","volume":"16","author":"LE Samartino","year":"1993","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"ref5","unstructured":"Acha PN, Szyfres B (2003) Zoonoses and communicable diseases common to man and animals. 3rd ed. Washington: Pan American Health Organization, 3v. (Scientific and Technical Publication, 580)."},{"key":"ref6","unstructured":"Nicoletti P (1990) Bovine abortion caused by <italic>Brucella<\/italic> sp. In: Kirkbride CA (Ed.) Laboratory Diagnosis of Livestock Abortion 3rd Ed. Ames: Iowa State University Press. p. 22\u201326."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jcpa.2008.10.004","article-title":"Pathology, immunohistochemistry and bacteriology of tissues and milk of cows and fetuses experimentally infected with <italic>Brucella abortus<\/italic>","volume":"140","author":"MN Xavier","year":"2009","journal-title":"J Comp Pathol"},{"key":"ref8","first-page":"226","article-title":"Ultrastructural morphometric analysis of <italic>Brucella abortus<\/italic>-infected trophoblasts in experimental placentitis. Bacterial replication occurs in rough endoplasmic reticulum","volume":"124","author":"T Anderson","year":"1986","journal-title":"Am J Pathol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1177\/030098588902600501","article-title":"Pathogenesis of <italic>Brucella abortus<\/italic> infection of the mammary gland and supramammary lymph node of the goat","volume":"26","author":"VP Meador","year":"1989","journal-title":"Vet Pathol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0147-9571(95)00018-6","article-title":"<italic>Brucella abortus<\/italic> differs in the multiplication within bovine chorioallantoic membrane explants from early and late gestation","volume":"19","author":"LE Samartino","year":"1996","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tvjl.2009.04.010","article-title":"Pathogenesis of bovine brucellosis","volume":"184","author":"AV Carvalho Neta","year":"2010","journal-title":"Vet J"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"359","DOI":"10.2460\/ajvr.1992.53.3.359","article-title":"Interaction of bovine chorioallantoic membrane explants with three strains of <italic>Brucella abortus<\/italic>","volume":"53","author":"LE Samartino","year":"1992","journal-title":"Am J Vet Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1128\/IAI.01554-07","article-title":"Modulation of bovine trophoblastic innate immune response by <italic>Brucella abortus<\/italic>","volume":"76","author":"AV Carvalho Neta","year":"2008","journal-title":"Infect Immun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1073\/pnas.032514299","article-title":"The <italic>Brucella suis virB<\/italic> operon is induced intracellularly in macrophages","volume":"99","author":"ML Boschiroli","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0378-1135(02)00219-5","article-title":"Type IV secretion and <italic>Brucella<\/italic> virulence","volume":"90","author":"ML Boschiroli","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"47","DOI":"10.2217\/fmb.11.136","article-title":"Brucellosis and type IV secretion","volume":"7","author":"MF De Jong","year":"2012","journal-title":"Future Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"5169","DOI":"10.1016\/j.vaccine.2006.04.005","article-title":"Evaluation of novel <italic>Brucella melitensis<\/italic> unmarked deletion mutants for safety and efficacy in the goat model of brucellosis","volume":"24","author":"MM Kahl-McDonagh","year":"2006","journal-title":"Vaccine"},{"key":"ref18","first-page":"399","article-title":"Subversion of Toll-like receptor signaling by a unique family of bacterial Toll\/interleukin-1 receptor domain\u2013containing proteins","volume":"14","author":"C Cirl","year":"2008","journal-title":"Nature"},{"key":"ref19","first-page":"3","article-title":"BtpB a novel <italic>Brucella<\/italic> TIR-containing effector protein with immune modulatory functions","volume":"8","author":"SP Salcedo","year":"2013","journal-title":"Front Cell Infect Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1371\/journal.ppat.0040021","article-title":"<italic>Brucella<\/italic> control of dendritic cell maturation is dependent on the TIR-Containing Protein Btp1","volume":"4","author":"SP Salcedo","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"5143","DOI":"10.1128\/IAI.72.9.5143-5149.2004","article-title":"Differential requirements for virB1 and virB2 during <italic>Brucella abortus<\/italic> infection","volume":"72","author":"AB Den Hartigh","year":"2004","journal-title":"Infect Immun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6048","DOI":"10.1128\/IAI.73.9.6048-6054.2005","article-title":"<italic>Brucella abortus virB12<\/italic> is expressed during infection but is not an essential component of the type IV secretion system","volume":"73","author":"YH Sun","year":"2005","journal-title":"Infect Immun"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1128\/IAI.01109-10","article-title":"Putative ATP-binding cassette transporter is essential for <italic>Brucella ovis<\/italic> pathogenesis in mice","volume":"79","author":"TM Silva","year":"2011","journal-title":"Infect Immun"},{"key":"ref24","first-page":"11","article-title":"Prenatal development of domestic and laboratory mammals: growth curves, external features and selected references","volume":"2","author":"HE Evans","year":"1973","journal-title":"Zentralbl Veterinarmed"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2<sup>\u2212\u0394\u0394Ct<\/sup> method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1084\/jem.20030088","article-title":"<italic>Brucella<\/italic> evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum","volume":"198","author":"J Celli","year":"2003","journal-title":"J Exp Med"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1073\/pnas.0406873102","article-title":"<italic>Brucella<\/italic> coopts the small GTPase Sar1 for intracellular replication","volume":"102","author":"J Celli","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1111\/j.1600-0854.2008.00718.x","article-title":"<italic>Brucella<\/italic> intracellular replication requires trafficking through the late endosomal\/lysosomal compartment","volume":"9","author":"T Starr","year":"2008","journal-title":"Traffic"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"6002","DOI":"10.4049\/jimmunol.1100232","article-title":"Phagosomal degradation increases TLR access to bacterial ligands and enhances macrophage sensitivity to bacteria","volume":"187","author":"AJ Wolf","year":"2011","journal-title":"J Immunol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1600-0897.2010.00904.x","article-title":"Maternal and fetal response to fetal persistent infection with bovine viral diarrhea virus","volume":"64","author":"TR Hansen","year":"2010","journal-title":"Am J Reprod Immunol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s13577-012-0058-z","article-title":"Dynamic change of TNIK in response to tumor necrosis factor alpha in a TRAF2-dependent manner","volume":"26","author":"J Gui","year":"2013","journal-title":"Hum Cell"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e75473","DOI":"10.1371\/journal.pone.0075473","article-title":"The expression and localization of N-myc downstream regulated gene 1 in human trophoblasts","volume":"8","author":"XH Shi","year":"2013","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/75977","article-title":"Mutations in SPINK5, encoding a serine protease inhibitor, cause Netherton syndrome","volume":"25","author":"S Chavanas","year":"2000","journal-title":"Nat Genet"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1093\/hmg\/ddg247","article-title":"LEKTI proteolytic processing in human primary keratinocytes, tissue distribution and defective expression in Netherton syndrome","volume":"12","author":"E Bitoun","year":"2003","journal-title":"Hum Mol Genet"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1038\/sj.jid.5700284","article-title":"Corneodesmosomal cadherins are preferential targets of stratum corneum trypsin-like and chymotrypsin-like hyperactivity in Netherton syndrome","volume":"126","author":"P Descargues","year":"2006","journal-title":"J Invest Dermatol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1128\/IAI.01356-12","article-title":"Toll-like receptor 6 plays an important role in host innate resistance to <italic>Brucella abortus<\/italic> infection in mice","volume":"81","author":"LA Almeida","year":"2013","journal-title":"Infect Immun"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2180-8-212","article-title":"Heat shock cognate protein 70 contributes to <italic>Brucella<\/italic> invasion into trophoblast giant cells that cause infectious abortion","volume":"8","author":"K Watanabe","year":"2008","journal-title":"BMC Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2007.11.023","article-title":"<italic>Brucella<\/italic>: A pathogen without classic virulence genes","volume":"129","author":"MN Seleem","year":"2007","journal-title":"Vet Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.mib.2003.11.001","article-title":"Organelle robbery: <italic>Brucella<\/italic> interactions with the endoplasmic reticulum","volume":"7","author":"J Celli","year":"2004","journal-title":"Curr Opin Microbiol"},{"key":"ref40","unstructured":"Mcgee DW (1999) Inflammation and mucosal cytokine production. In: OGRA PL. Mucosal immunology 2nd ed. San Diego: Academic Press, p. 559\u2013573."},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e62257","DOI":"10.1371\/journal.pone.0062257","article-title":"The complement anaphylatoxin C3a receptor (C3aR) contributes to the inflammatory response in Dextran Sulfate Sodium (DSS)-induced colitis in mice","volume":"8","author":"E Wende","year":"2013","journal-title":"PLoS One"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0165-2427(94)90103-1","article-title":"Mechanism of binding of <italic>Brucella abortus<\/italic> to mononuclear phagocytes from cows naturally resistant or susceptible to brucellosis","volume":"41","author":"GA Campbell","year":"1994","journal-title":"Vet Immunol Immunopathol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0378-1135(02)00214-6","article-title":"<italic>Brucella<\/italic> intracellular life: from invasion to intracellular replication","volume":"90","author":"JP Gorvel","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"3702","DOI":"10.1128\/IAI.73.6.3702-3713.2005","article-title":"Opsonized virulent <italic>Brucella abortus<\/italic> replicates within nonacidic, endoplasmic reticulum-negative, LAMP-1-positive phagosomes in human monocytes","volume":"73","author":"BH Bellaire","year":"2005","journal-title":"Infect Immun"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.virol.2005.02.018","article-title":"The transcriptome of HCV replicon expressing cell lines in the presence of alpha interferon","volume":"335","author":"J Hayashi","year":"2005","journal-title":"Virology"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S1535-6108(03)00241-1","article-title":"Vsv strains with defects in their ability to shutdown innate immunity are potent systemic anti-cancer agents","volume":"4","author":"DF Stojdl","year":"2003","journal-title":"Cancer Cell"},{"key":"ref47","first-page":"256","article-title":"Microarray analysis of primary endothelial cells challenged with different inflammatory and immune cytokines","volume":"29","author":"TR Sana","year":"2005","journal-title":"Cytokine"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1006\/geno.2002.6729","article-title":"The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue","volume":"79","author":"H Monajemi","year":"2002","journal-title":"Genomics"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1111\/j.1462-5822.2007.00922.x","article-title":"<italic>Brucella<\/italic> requires a functional Type IV secretion system to elicit innate immune responses in mice","volume":"9","author":"CM Roux","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref50","first-page":"1","article-title":"Transducers of life and death: TNF receptor superfamily and associated proteins","volume":"12","author":"SJ Baker","year":"1996","journal-title":"Oncogene"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.smim.2006.04.006","article-title":"BAFF, APRIL and their receptors: structure, function and signaling","volume":"18","author":"C Bossen","year":"2006","journal-title":"Semin Immunol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.trim.2007.05.012","article-title":"Role of soluble tumor necrosis factor-related apoptosis-inducing ligand concentrations after stem cell transplantation","volume":"18","author":"S Nomura","year":"2007","journal-title":"Transpl Immunol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s002510050534","article-title":"A radiation hybrid map of complement factor H and factor H-related genes","volume":"49","author":"MA Diaz-Guillen","year":"1999","journal-title":"Immunogenetics"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0161-5890(00)00024-9","article-title":"Complement factor H: sequence analysis of 221 kb of human genomic DNA containing the entire fH, fHR-1 and fHR-3 genes","volume":"37","author":"DA Male","year":"2000","journal-title":"Mol Immunol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"3316","DOI":"10.1172\/JCI62070","article-title":"C\/EBP\u03b5 mediates nicotinamide enhanced clearance of <italic>Staphylococcus aureus<\/italic> in mice","volume":"122","author":"P Kyme","year":"2012","journal-title":"J Clin Invest"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0108606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T01:05:35Z","timestamp":1717376735000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0108606"}},"subtitle":[],"editor":[{"given":"Odir A.","family":"Dellagostin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,9,26]]},"references-count":55,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2014,9,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0108606","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,26]]}}}