{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T12:18:36Z","timestamp":1792498716628,"version":"4.5.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2014,10,10]],"date-time":"2014-10-10T00:00:00Z","timestamp":1412899200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0109219","type":"journal-article","created":{"date-parts":[[2014,10,10]],"date-time":"2014-10-10T18:09:27Z","timestamp":1412964567000},"page":"e109219","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Impact of Exogenic Testosterone and Nortestosterone-Decanoate Toxicological Evaluation Using a Rat Model"],"prefix":"10.1371","volume":"9","author":[{"given":"Romeo Teodor","family":"Cristina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Hanganu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Dumitrescu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florin","family":"Muselin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Butnariu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Constantin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viorica","family":"Chiurciu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,10,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.reprotox.2003.10.012","article-title":"Evaluation of the rodent Hershberger assay using three reference endocrine disrupters (androgen and antiandrogens)","volume":"18","author":"PF Kennel","year":"2004","journal-title":"Reprod Toxicol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1289\/ehp.9357","article-title":"Ten years of mixing cocktails: a review of combination effects of endocrine-disrupting chemicals","volume":"115","author":"A Kortenkamp","year":"2007","journal-title":"Environ Health Perspect"},{"key":"ref3","unstructured":"Pritchard JD (2007) Review of chemical toxicity to the reproductive system, with particular reference to developmental toxicity \u2013 Health Protection Agency: HPA-ChaPD-002. Available: http:\/\/www.hpa.org.uk\/webc\/HPAwebFile\/HPAweb_C\/1194947320972<\/ext-link>. Accessed 2013 May 22."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/S0149-2918(01)80114-4","article-title":"A review of chemistry, biological action, and clinical applications of anabolic-androgenic steroids","volume":"23","author":"NA Shahidi","year":"2001","journal-title":"Clin Ther"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1039\/b901813a","article-title":"Development of a metabolomic approach based on liquid chromatography-high resolution mass spectrometry to screen for clenbuterol abuse in calves","volume":"134","author":"F Courant","year":"2009","journal-title":"Analyst"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1530\/acta.0.1010108","article-title":"Influence of nandrolone decanoate on the pituitary-gonadal axis in males","volume":"101","author":"JW Bijlsma","year":"1982","journal-title":"Acta Endocrinol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0003-2670(93)80281-O","article-title":"Urinary excretion of androst\u00e8nedione in cattle as an indicator of possible illegal use in livestock breeding","volume":"275","author":"GC Gatti","year":"1993","journal-title":"Anal Chim Acta"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1080\/02652030802627160","article-title":"Presence and metabolism of endogenous androgenic\u2013anabolic steroid hormones in meat-producing animals: a review","volume":"26","author":"J Scarth","year":"2009","journal-title":"Food Addit Contam Part A Chem Anal Control Expo Risk Assess"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1080\/19440049.2010.539628","article-title":"Detection of endogenous steroid abuse in cattle: results from population studies in the UK","volume":"28","author":"JP Scarth","year":"2011","journal-title":"Food Addit Contam Part A Chem Anal Control Expo Risk Assess"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1051\/rnd:19930401","article-title":"Revue bibliographique quantitative sur l'utilisation des hormones anabolisantes \u00e0 action st\u00e9ro\u00efdienne chez les ruminants en production de viande. II. Principaux modes d'action","volume":"33","author":"P Schmidley","year":"1993","journal-title":"Reprod Nutr Dev"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1016\/S0024-3205(01)00972-9","article-title":"Effects of androgenic anabolic steroids on sperm quality and serum hormone levels in adult male bodybuilders","volume":"68","author":"J Torres-Calleja","year":"2001","journal-title":"Life Sci"},{"key":"ref12","unstructured":"EDSTAC (2011) Endocrine Disruptor Screening and Testing Advisory Committee - Final Report, Available: http:\/\/www.epa.gov\/endo\/pubs\/edspoverview\/finalrpt.htm<\/ext-link> Accessed 22 May 2013"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0378-4347(98)00541-6","article-title":"Evidence for presence of endogenous 19-norandrostenedione in human urine","volume":"723","author":"B Le Bizec","year":"1990","journal-title":"J Chromatogr B"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.5713\/ajas.2010.10278","article-title":"Feeding effect of an anabolic steroid, nandrolone, on the male rat testis","volume":"23","author":"L Dong-Mok","year":"2010","journal-title":"Asian-Aust J Anim Sci"},{"key":"ref15","unstructured":"(2011) RA 1235 OSSmPC 9. Deca-durabolin (nandrolone decanoate) - Product information. Available: http:\/\/www.medicines.org.au\/files\/mkpdecad.pdf<\/ext-link>. Accessed 2013 May 22."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/BF02210686","article-title":"Nandrolone decanoate: Pharmacological properties and therapeutic use in osteoporosis","volume":"14","author":"P Geusens","year":"1995","journal-title":"Clin Rheumatol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1001\/archinte.165.5.578","article-title":"Effect of nandrolone decanoate therapy on weight and lean body mass in HIV-infected women with weight loss: a randomized, double-blind, placebo-controlled, multicenter trial","volume":"165","author":"K Mulligan","year":"2005","journal-title":"Arch Intern Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.5713\/ajas.2010.10278","article-title":"Feeding effect of an anabolic steroid, nandrolone, on the male rat testis","volume":"23","author":"DM Lee","year":"2010","journal-title":"Asian-Aust J Anim Sci"},{"key":"ref19","unstructured":"OECD (2013) Organisation for Economic Co-operation and Development (2011) Guidelines for the Testing of Chemicals Test No. 441 Hershberger Bioassay in Rats. Available: http:\/\/www.oecd.org\/env\/ehs\/testing\/37478355.pdf<\/ext-link>. Accessed 2013 May 22"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s00204-008-0357-z","article-title":"Uterotrophic assay, Hershberger assay, and subacute oral toxicity study of 3-amino-1,2,4-triazole based on the Organization for Economic Co-operation and Development draft protocols","volume":"83","author":"T Umano","year":"2009","journal-title":"Arch Toxicol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1093\/toxsci\/kfh093","article-title":"Enhaced rat Hershberger assay appears reliable for detection of not only (anti)-androgenic chemicals but also thyroid hormone modulators","volume":"79","author":"TY Yamada","year":"2004","journal-title":"Toxicol Sci"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1007\/s00204-001-0296-4","article-title":"Uterotrophic and Hershberger assay for n-buthyl benzene in rats","volume":"75","author":"K Yamasaki","year":"2002","journal-title":"Arch Toxicol"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Gray L, Furr J, Ostby JS (2005) Hershberger assay to investigate the effects of endocrine disrupting compounds with androgenic and antiandrogenic activity in castrate-immature male rats. In Current Protocols in Toxicology 16.9.1\u201316.9.15, J Wiley and Sons Inc.","DOI":"10.1002\/0471140856.tx1609s26"},{"key":"ref24","first-page":"131","article-title":"Evaluation of the rodent Hershberger bioassay using three reference (anti)androgens","volume":"56","author":"A Freyberger","year":"2005","journal-title":"Arh Hig Rada Toksikol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1289\/ehp.8751","article-title":"The OECD program to validate the rat Hershberger bioassay to screen compounds for in vivo androgen and antiandrogen responses. Phase 1: use of a potent agonist and a potent antagonist to test the standardized protocol","volume":"114","author":"W Owens","year":"2006","journal-title":"Env Health Perspect"},{"key":"ref26","unstructured":"European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (ECPVAEOSP) (1986) European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes - Council of Europe. Available: http:\/\/conventions.coe.int\/treaty\/en\/treaties\/html\/123.htm<\/ext-link>. Accessed 2013 May 22."},{"key":"ref27","unstructured":"(2010) Directive 2010\/63\/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Available: http:\/\/eur-lex.europa.eu\/LexUriServ\/LexUriServ.do?uri=CELEX:32010L0063<\/ext-link>. Accessed 2013 May 22."},{"key":"ref28","unstructured":"(2002) Romanian Law no. 471 of 9 July 2002 approving Government Ordinance no. 37\/2002 for the protection of animals used for scientific or other experimental purposes. Available: http:\/\/www.aulim.ro\/ro\/doc\/legislatie\/471_2002.pdf<\/ext-link>. Accessed 2013 May 22."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1289\/ehp.8670149","article-title":"Detection of alteration in testicular and epididymal function in laboratory animals","volume":"70","author":"P Amann","year":"1986","journal-title":"Env Health Perspect"},{"key":"ref30","unstructured":"Mate? N (2001) Chirurgie operatoare veterinar\u0103 (in Romanian) Ed. Risoprint, Cluj-Napoca, pp 483."},{"key":"ref31","unstructured":"Peirce S (2006) SVH AEC SOP. 26. Euthanasia of mice and rats. Available: www.pcarp.usp.br<\/ext-link>. Accessed 2013 May 22."},{"key":"ref32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1752-153X-6-22","article-title":"Rapid determination of natural steroidal hormones in saliva for the clinical diagnoses","volume":"6","author":"O Jin-Aa","year":"2012","journal-title":"Chem Cent J"},{"key":"ref33","unstructured":"Free T4 Architect System (2010) Available: http:\/\/www.ilexmedical.com\/files\/PDF\/FreeT4_ARC.pdf<\/ext-link>. Accessed 2013 May 22."},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0165-0173(96)00002-1","article-title":"A review of brain aromatase cytochrome P450","volume":"22","author":"ED Lephart","year":"1996","journal-title":"Brain Res Rev"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1093\/clinchem\/42.7.1001","article-title":"Metabolism of anabolic androgenic steroids","volume":"42","author":"W Sch\u00e4nzer","year":"1996","journal-title":"Clin Chem"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1172\/JCI116665","article-title":"Tissue distribution and ontogeny of steroid 5-alpha-reductase isozyme expression","volume":"92","author":"AE Thigpen","year":"1993","journal-title":"J Clin Invest"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1745-7262.2007.00203.x","article-title":"The reversibility of sperm quality after discontinuing nandrolone decanoate in adult male rats","volume":"9","author":"S Karbalay-Doust","year":"2007","journal-title":"Asian J Androl"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1507\/endocrj.51.425","article-title":"Endocrinological and pathological effects of anabolic-androgenic steroid in male rats","volume":"51","author":"M Takahashi","year":"2004","journal-title":"Endocr J"},{"key":"ref39","first-page":"39","article-title":"The effect of orchidectomy and administration of testosterone propionate or nandrolone phenyl propionate to orchidectomised rats on their growth and carcass composition","volume":"3","author":"DH Hale","year":"1972","journal-title":"S Afr J Anim Sci"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.taap.2007.08.023","article-title":"Androgenic endocrine disruptors in wastewater plant effluents in India: Their influence on reproductive processes and systemic toxicity in male rats","volume":"226","author":"V Kumar","year":"2008","journal-title":"Toxicol Appl Pharmacol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/0022-4731(85)90215-8","article-title":"Pharmacokinetics of 19-nortestosterone esters in normal men","volume":"22","author":"L Belkien","year":"1985","journal-title":"J Steroid Biochem"},{"key":"ref42","article-title":"Effect of nandrolone decanoate on serum FSH, LH and testosterone concentration in male albino mice","author":"S Purkayastha","year":"2012","journal-title":"WJLSMR"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0109219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T18:09:09Z","timestamp":1598378949000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0109219"}},"subtitle":[],"editor":[{"given":"Bin","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,10,10]]},"references-count":42,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2014,10,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0109219","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,10]]}}}