{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T03:14:06Z","timestamp":1771816446916,"version":"3.50.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T00:00:00Z","timestamp":1415232000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0111244","type":"journal-article","created":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T19:19:37Z","timestamp":1415301577000},"page":"e111244","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["In Vitro, In Silico and In Vivo Studies of Ursolic Acid as an Anti-Filarial Agent"],"prefix":"10.1371","volume":"9","author":[{"given":"Komal","family":"Kalani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikas","family":"Kushwaha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pooja","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richa","family":"Verma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mukesh","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feroz","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. K.","family":"Murthy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santosh Kumar","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2014,11,6]]},"reference":[{"key":"ref1","unstructured":"WHO, 2012. Global Programme to Eliminate Lymphatic Filariasis. Weekly Epidemiological Record. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/apps.who.int\/iris\/bitstream\/10665\/78611\/1\/WHO_HTM_NTD_PCT_2013.5_eng.pdf\" xlink:type=\"simple\">http:\/\/apps.who.int\/iris\/bitstream\/10665\/78611\/1\/WHO_HTM_NTD_PCT_2013.5_eng.pdf<\/ext-link>. Accessed 16 July 2014."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1179\/000349802125002374","article-title":"Lymphatic filariasis elimination: Progress in global programme development","volume":"96","author":"DH Molyneux","year":"2002","journal-title":"Ann Trop Med Parasitol"},{"key":"ref3","unstructured":"WHO, 2006. Global programme to eliminate lymphatic filariasis. Weekly epidemiological record, Geneva. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/lymphatic_filariasis\/resources\/wer\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/lymphatic_filariasis\/resources\/wer\/en\/<\/ext-link>. Accessed 22 January 2013."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1046\/j.1365-3156.2000.00620.x","article-title":"The global programme to eliminate lymphatic filariasis","volume":"5","author":"EA Ottesen","year":"2000","journal-title":"Trop Med Int Hlth"},{"key":"ref5","unstructured":"WHO, 2005. Global programme to eliminate lymphatic filariasis. Weekly epidemiological record. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/lymphatic_filariasis\/resources\/wer\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/lymphatic_filariasis\/resources\/wer\/en\/<\/ext-link>. Accessed 22 April 2014."},{"key":"ref6","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.pt.2003.09.004","article-title":"Mass drug treatment for lymphatic filariasis and onchocerciasis","volume":"19","author":"DH Molyneux","year":"2003","journal-title":"Trends Parasitol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1475-2883-2-2","article-title":"Final report of the conference on the eradicability of Onchocerciasis","volume":"2","author":"Y Dadzie","year":"2003","journal-title":"Filaria J"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1475-2883-5-10","article-title":"The argument for integrating vector control with multiple drug administration campaigns to ensure elimination of lymphatic filariasis","volume":"5","author":"TR Burkot","year":"2006","journal-title":"Filaria J"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2566","DOI":"10.1016\/j.bmcl.2013.02.115","article-title":"Glycyrrhetinic acid and its analogs: A new class of antifilarial agents","volume":"23","author":"K Kalani","year":"2013","journal-title":"Bioorg Med Chem Lett"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1055\/s-0030-1250452","article-title":"Plant products in the treatment and control of filariasis and other helminth infections and assay systems for antifilarial\/antihelmintic activity","volume":"77","author":"PK Murthy","year":"2011","journal-title":"Planta Med"},{"key":"ref11","first-page":"202","article-title":"Antifilarial activity of gum from Moringa oleifera Lam. on human lymphatic filaria <italic>Brugia Malayi<\/italic>","author":"V Kushwaha","year":"2011","journal-title":"Chronicles of Young Scientists"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1590\/S0103-50532012000300013","article-title":"Determination of ursolic acid and ursolic acid lactone in the leaves of <italic>Eucalyptus tereticornis<\/italic> by HPLC","volume":"23","author":"A Maurya","year":"2012","journal-title":"J Braz Chem Soc"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1007\/s00894-011-1327-6","article-title":"Pharmacophore, QSAR, and ADME based semisynthesis and <italic>in vitro<\/italic> evaluation of ursolic acid analogs for anticancer activity","volume":"18","author":"K Kalani","year":"2012","journal-title":"J Mol Model"},{"key":"ref14","first-page":"623","article-title":"Susceptibility of Mastomys natalensis (GRA strain) to a sub periodic strain of human <italic>Brugia malayi.<\/italic>","volume":"77","author":"PK Murthy","year":"1983","journal-title":"Indian J Med Res"},{"key":"ref15","first-page":"302","article-title":"Fate of infective larvae of <italic>Brugia malayi<\/italic> in the peritoneal cavity of Mastomys natalensis and Meriones unguiculatus","volume":"44","author":"PK Murthy","year":"1997","journal-title":"Folia Parasitol (Praha)"},{"key":"ref16","first-page":"1084","article-title":"Evaluation of two <italic>in vitro<\/italic> test systems employing <italic>Brugia malayi<\/italic> parasite for screening of potential antifilarials","volume":"77","author":"PK Murthy","year":"1999","journal-title":"Curr Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.actatropica.2010.06.006","article-title":"Antifilarial activity in vitro and in vivo of some flavonoids tested against <italic>Brugia malayi<\/italic>","volume":"116","author":"V Lakshmi","year":"2010","journal-title":"Acta Trop"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.ejmech.2014.05.029","article-title":"Synthesis and antifilarial activity of chalcone-thiazole derivatives against a human lymphatic filarial parasite, <italic>Brugia malayi.<\/italic>","volume":"81","author":"KV Sashidhara","year":"2014","journal-title":"Eur J Med Chem"},{"key":"ref19","first-page":"473","article-title":"A new fluorimetric assay for cytotoxicity measurements <italic>in vitro<\/italic>","volume":"3","author":"C Page","year":"1993","journal-title":"Int J Oncol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"T Mosmann","year":"1983","journal-title":"J Immunol Methods"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0001-706X(93)90083-N","article-title":"A comparison of three methods of estimating EC<sub>50<\/sub> in studies of drug resistance of malaria parasites","volume":"55","author":"W Huber","year":"1993","journal-title":"Acta Trop"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S1093-3263(98)00002-3","article-title":"LIGSITE: automatic and efficient detection of potential small molecule-binding sites in proteins","volume":"15","author":"M Hendlich","year":"1997","journal-title":"J Mol Graph Model"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1002\/ddr.430260106","article-title":"Macrofilaricidal activity of the stem bark of Streblus asper and its major active constituents","volume":"26","author":"RK Chatterjee","year":"1992","journal-title":"Drug Develop Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1017\/S0031182006001612","article-title":"Antifilarial activity of novel formulations of albendazole against experimental brugian filariasis","volume":"134","author":"RL Gaur","year":"2007","journal-title":"Parasitology"},{"key":"ref25","first-page":"205","article-title":"Chemo prophylactic activity of filaricidal compounds on Litomosoides carinii infection of Mastomys natalensis","volume":"28","author":"G L\u00e4mmler","year":"1977","journal-title":"Tropen Med Parasitol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S1359-6446(03)02971-4","article-title":"Early prediction of drug metabolism and toxicity: systems biology approach and modeling","volume":"9","author":"A Bugrim","year":"2004","journal-title":"Drug Discov Today"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.phymed.2012.10.017","article-title":"Antifilarial diarylheptanoids from Alnus nepalensis leaves growing in high altitude areas of Uttarakhand, India","volume":"20","author":"D Yadav","year":"2013","journal-title":"Phytomedicine"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s00894-011-1035-2","article-title":"Virtual screening and <italic>in vitro<\/italic> assay of potential drug like inhibitors from spices against glutathione-S-transferase of filarial nematodes","volume":"18","author":"S Azeez","year":"2012","journal-title":"J Mol Model"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.2174\/1568026614666140324121606","article-title":"QSAR guided semi-synthesis and <italic>in-vitro<\/italic> validation of anticancer activity in ursolic acid derivatives","volume":"14","author":"K Kalani","year":"2014","journal-title":"Curr Top Med Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"5","DOI":"10.12691\/ajidm-1-1-2","article-title":"The Curse of Lymphatic Filariasis: Would the Continual Use of Diethylcarbamazine Eliminate this Scourge in Papua New Guinea?","volume":"1","author":"A Lanham","year":"2013","journal-title":"American Journal of Infectious Diseases and Microbiology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"7718","DOI":"10.1128\/IAI.69.12.7718-7728.2001","article-title":"Structural analysis and antibody response to the extracellular glutathione S-transferases from Onchocerca volvulus","volume":"69","author":"A Sommer","year":"2001","journal-title":"Infect Immun"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1006\/expr.1994.1067","article-title":"Parasitic helminth glutathione S-transferases: an update on their potential as targets for immuno- and chemotherapy","volume":"79","author":"PM Brophy","year":"1994","journal-title":"Exp Parasitol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0111244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T05:47:47Z","timestamp":1589003267000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0111244"}},"subtitle":[],"editor":[{"given":"Gnanasekar","family":"Munirathinam","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,11,6]]},"references-count":32,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2014,11,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0111244","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,6]]}}}