{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:05Z","timestamp":1789171205298,"version":"build-2803163510"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T00:00:00Z","timestamp":1419811200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate whether a combination of demineralized bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) infected with adenovirus-mediated- bone morphogenetic protein (Ad-BMP-2) and transforming growth factor-\u03b23 (Ad-TGF-\u03b23) promotes the repair of the full-thickness cartilage lesions in pig model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>BMSCs isolated from pig were cultured and infected with Ad-BMP-2(B group), Ad-TGF-\u03b23 (T group), Ad-BMP-2 + Ad-TGF-\u03b23(BT group), cells infected with empty Ad served as a negative group(N group), the expression of the BMP-2 and TGF-\u03b23 were confirmed by immunofluorescence, PCR, and ELISA, the expression of SOX-9, type II collagen(COL-2A), aggrecan (ACAN) in each group were evaluated by real-time PCR at 1w, 2w, 3w, respectively. The chondrogenic differentiation of BMSCs was evaluated by type II collagen at 21d with immunohistochemical staining. The third-passage BMSCs infected with Ad-BMP-2 and Ad-TGF-\u03b23 were suspended and cultured with DBM for 6 days to construct a new type of tissue engineering scaffold to repair full-thickness cartilage lesions in the femur condyles of pig knee, the regenerated tissue was evaluated at 1,2 and 3 months after surgery by gross appearance, H&amp;E, safranin O staining and O'driscoll score.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ad-BMP-2 and Ad-TGF-\u03b23 (BT group) infected cells acquired strong type II collagen staining compared with Ad-BMP-2 (B group) and Ad-TGF-\u03b23 (T group) along. The Ad-BMP-2 and Ad-TGF-\u03b23 infected BMSCs adhered and propagated well in DBM and the new type of tissue engineering scaffold produced hyaline cartilage morphology containing a stronger type II collagen and safranin O staining, the O'driscoll score was higher than other groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The DBM compound with Ad-BMP-2 and Ad-TGF-\u03b23 infected BMSCs scaffold has a good biocompatibility and could well induce cartilage regeneration to repair the defects of joint cartilage. This technology may be efficiently employed for cartilage lesions repair\n                      <jats:italic>in vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0116061","type":"journal-article","created":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T13:57:37Z","timestamp":1419861457000},"page":"e116061","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":45,"title":["RETRACTED: Demineralized Bone Matrix Combined Bone Marrow Mesenchymal Stem Cells, Bone Morphogenetic Protein-2 and Transforming Growth Factor-\u03b23 Gene Promoted Pig Cartilage Defect Repair"],"prefix":"10.1371","volume":"9","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanlin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,12,29]]},"reference":[{"key":"pone.0116061-Redman1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.22203\/eCM.v009a04","article-title":"Current strategies for articular cartilage repair","volume":"9","author":"SN Redman","year":"2005","journal-title":"Eur Cell Mater"},{"key":"pone.0116061-Santhagunam1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1002\/bab.1016","article-title":"Genetically engineered stem cell-based strategies for articular cartilage regeneration","volume":"59","author":"A Santhagunam","year":"2012","journal-title":"Biotechnol Appl Biochem"},{"key":"pone.0116061-Aulin1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1177\/0023677212473716","article-title":"Cartilage repair of experimentally 11 induced osteochondral defects in New Zealand White rabbits","volume":"47","author":"C Aulin","year":"2013","journal-title":"Lab Anim"},{"key":"pone.0116061-Muller1","first-page":"111","article-title":"Results of osteochondral autologous transplantation in the knee","volume":"4","author":"S Muller","year":"2010","journal-title":"Open Orthop J"},{"key":"pone.0116061-Becher1","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1007\/s00167-009-1036-1","article-title":"Microfracture for chondral defects of the talus: maintenance of early results at midterm follow-up","volume":"18","author":"C Becher","year":"2010","journal-title":"Knee Surg Sports Traumatol Arthrosc"},{"key":"pone.0116061-Hui1","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1016\/j.arthro.2013.06.009","article-title":"Cartilage repair: 2013 Asian update","volume":"29","author":"JH Hui","year":"2013","journal-title":"Arthroscopy"},{"key":"pone.0116061-ODriscoll1","doi-asserted-by":"crossref","unstructured":"O'Driscoll SW (2001) Preclinical cartilage repair: current status and future perspectives. 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