{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:29:40Z","timestamp":1762252180157},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2015,2,24]],"date-time":"2015-02-24T00:00:00Z","timestamp":1424736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0118736","type":"journal-article","created":{"date-parts":[[2015,2,24]],"date-time":"2015-02-24T18:50:08Z","timestamp":1424803808000},"page":"e0118736","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Two Cathepsins B Are Responsible for the Yolk Protein Hydrolysis in Culex quinquefasciatus"],"prefix":"10.1371","volume":"10","author":[{"given":"Alexandre S.","family":"Moura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 F.","family":"Cardoso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 L.","family":"Costa-da-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos E.","family":"Winter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Tania","family":"Bijovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,2,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1099\/0022-1317-82-8-1867","article-title":"Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography","volume":"82","author":"MW Gaunt","year":"2001","journal-title":"J Gen Virol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1089\/vbz.2006.0589","article-title":"Geographic variation in vector competence for West Nile virus in the Culex pipiens (Diptera: Culicidae) complex in California","volume":"7","author":"R Vaidyanathan","year":"2007","journal-title":"Vector Borne Zoonot"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1093\/jmedent\/45.1.125","article-title":"Culex pipiens (Diptera: Culicidae): A bridge vector of West Nile virus to humans","volume":"45","author":"GL Hamer","year":"2008","journal-title":"J Med Entomol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1111\/tmi.12277","article-title":"Vector competence of Culex pipiens quinquefasciatus (Diptera: Culicidae) for West Nile virus isolates from Florida","volume":"19","author":"SL Richards","year":"2014","journal-title":"Trop Med Int Health"},{"key":"ref5","author":"L Rey","year":"2008"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1083\/jcb.20.2.313","article-title":"Yolk protein uptake in the oocyte of the mosquito Aedes aegypti. L","volume":"20","author":"TF Roth","year":"1964","journal-title":"J Cell Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1146\/annurev.en.37.010192.001245","article-title":"Accumulation of yolk proteins in insect oocytes","volume":"37","author":"AS Raikhel","year":"1992","journal-title":"Annu Rev Entomol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/0003-9861(88)90646-7","article-title":"Drosophila cathepsin B-like proteinase: A suggested role in yolk degradation","volume":"263","author":"M Medina","year":"1988","journal-title":"Arch Biochem Biophys"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/0965-1748(93)90002-A","article-title":"Cathepsin B and acid phosphatase activities during Musca domestica embryogenesis","volume":"23","author":"PEM Ribolla","year":"1993","journal-title":"Insect Biochem Mol Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/0965-1748(95)00036-U","article-title":"Processing of procathepsin from Musca domestica eggs","volume":"25","author":"PEM Ribolla","year":"1995","journal-title":"Insect Biochem Mol Biol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"13311","DOI":"10.1074\/jbc.274.19.13311","article-title":"Mosquito cathepsin B-like protease involved in embryonic degradation of vitellin is produced as a latent extraovarian precursor","volume":"274","author":"WL Cho","year":"1999","journal-title":"J Biol Chem"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e22573","DOI":"10.1371\/journal.pone.0022573","article-title":"The fat body transcriptomes of the yellow fever mosquito Aedes aegypti, pre- and post- blood meal","volume":"6","author":"DP Price","year":"2011","journal-title":"PLoS One"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1093\/oxfordjournals.jbchem.a124165","article-title":"Cysteine proteinase from the eggs of the silkmoth, Bombyx mori: identification of a latent enzyme and characterization of activation and proteolytic processing in vivo and in vitro","volume":"114","author":"SY Takahashi","year":"1993","journal-title":"J Biochem"},{"key":"ref14","first-page":"591","article-title":"Autolytic activation mechanism of Bombyx acid cysteine protease (BCP)","volume":"42","author":"SY Takahashi","year":"1997","journal-title":"Biochem Mol Biol Int"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1080\/07924259.1996.9672553","article-title":"Bombyx acid cysteine proteinase","volume":"30","author":"SY Takahashi","year":"1996","journal-title":"Invertebrate Reprod Dev"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0305-0491(93)90004-O","article-title":"Cysteine proteinase from Bombyx eggs: role in programmed degradation of yolk proteins during embryogenesis","volume":"106","author":"Y Yamamoto","year":"1993","journal-title":"Comp Biochem Phys B"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/S0022-1910(99)00167-5","article-title":"Bombyx acid cysteine protease (BCP): hormonal regulation of biosynthesis and accumulation in the ovary","volume":"46","author":"Y Yamamoto","year":"2000","journal-title":"J Insect Physiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1093\/oxfordjournals.jbchem.a124683","article-title":"Molecular cloning and sequencing of cDNA that encodes cysteine proteinase in the eggs of the silkmoth, Bombyx mori","volume":"116","author":"Y Yamamoto","year":"1994","journal-title":"J Biochem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0965-1748(98)00015-0","article-title":"Purification and characterization of a cysteine proteinase from eggs of the cotton boll worm, Helicoverpa armigera","volume":"28","author":"XF Zhao","year":"1998","journal-title":"Insect Biochem Mol Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1046\/j.1365-2583.2002.00366.x","article-title":"Molecular cloning and characterization of the cathepsin B-like proteinase from the cotton boll worm, Helicoverpa armigera","volume":"11","author":"XF Zhao","year":"2002","journal-title":"Insect Mol Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s10930-005-6714-3","article-title":"Analysis of substrate specificity and endopeptidyl activities of the cathepsin B-like proteinase from Helicoverpa armigera","volume":"24","author":"XF Zhao","year":"2005","journal-title":"Protein J"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"33344","DOI":"10.1074\/jbc.271.52.33344","article-title":"A cysteine protease that processes insect vitellin. Purification and partial characterization of the enzyme and the proenzyme","volume":"271","author":"XD Liu","year":"1996","journal-title":"J Biol Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.jinsphys.2008.04.005","article-title":"Lima APCA, Machado EA. Interplay between acid phosphatase and cysteine proteases in mediating vitellin degradation during early embryogenesis of Periplaneta americana","volume":"54","author":"DMP Oliveira","year":"2008","journal-title":"J Insect Physiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1016\/j.jinsphys.2004.07.002","article-title":"Follicular epithelial cell apoptosis of atretic follicles within developing ovaries of the mosquito Culex pipiens pallens","volume":"50","author":"K Uchida","year":"2004","journal-title":"J Insect Physiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1006\/dbio.2001.0357","article-title":"Preoviposition activation of cathepsin-like proteinases in degenerating ovarian follicles of the mosquito Culex pipiens pallens","volume":"237","author":"K Uchida","year":"2001","journal-title":"Dev Biol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S1467-8039(03)00035-5","article-title":"Morphological aspects of Culex quinquefasciatus salivary glands","volume":"32","author":"Sais T da Cunha","year":"2003","journal-title":"Arthropod Struct Dev"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.ibmb.2004.02.008","article-title":"An insight into the salivary transcriptome and proteome of the adult female mosquito Culex pipiens quinquefasciatus","volume":"34","author":"JM Ribeiro","year":"2004","journal-title":"Insect Biochem Mol Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.jinsphys.2007.07.005","article-title":"Cell death and regeneration in the midgut of the mosquito, Culex quinquefasciatus","volume":"53","author":"K Okuda","year":"2007","journal-title":"J Insect Physiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0040-8166(02)00032-0","article-title":"Functional morphology of adult female <italic>Culex quinquefasciatus<\/italic> midgut during blood digestion","volume":"34","author":"K Okuda","year":"2002","journal-title":"Tissue Cell"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1365-2915.1994.tb00387.x","article-title":"Differential responses of Aedes and Culex mosquitoes to octenol or light in combination with carbon dioxide in Queensland, Australia","volume":"8","author":"PH Van Essen","year":"1994","journal-title":"Med Vet Entomol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1590\/S0074-02761999000100022","article-title":"Evaluation of insecticide resistance and biochemical mechanisms in a population of Culex quinquefasciatus (Diptera: Culicidae) from Sao Paulo, Brazil","volume":"94","author":"JE Bracco","year":"1999","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1042\/bj2010189","article-title":"L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L","volume":"201","author":"AJ Barrett","year":"1982","journal-title":"Biochem J"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0014-5793(91)80318-W","article-title":"Novel epoxysuccinyl peptides. Selective onhibitors of cathepsin-B, in vitro","volume":"280","author":"M Murata","year":"1991","journal-title":"FEBS Letters"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1038\/nprot.2006.468","article-title":"In-gel digestion for mass spectrometric characterization of proteins and proteomes","volume":"1","author":"A Shevchenko","year":"2006","journal-title":"Nat Protoc"},{"key":"ref37","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gks596","article-title":"Primer3-new capabilities and interfaces","volume":"40","author":"A Untergasser","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1673\/031.002.0701","article-title":"<italic>Aedes aegypti<\/italic> ferritin heavy chain homologue: feeding of iron or blood influences message levels, lengths and subunit abundance","volume":"2","author":"BC Dunkov","year":"2002","journal-title":"J Insect Sci"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0378-1119(88)90330-7","article-title":"CLUSTAL: a package for performing multiple sequence alignment on a microcomputer","volume":"73","author":"DG Higgins","year":"1988","journal-title":"Gene"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1142\/S0129065797000537","article-title":"A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites","volume":"8","author":"H Nielsen","year":"1997","journal-title":"Int J Neural Syst"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: Molecular Evolutionary Genetics Analysis version 6.0","volume":"30","author":"K Tamura","year":"2013","journal-title":"Mole Biol Evol"},{"issue":"Pt C","key":"ref43","first-page":"535","article-title":"Methods Enzymol","volume":"80","author":"AJ Barrett","year":"1981","journal-title":"Methods Enzymol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.jinsphys.2014.02.001","article-title":"Serosal cuticle formation and distinct degrees of desiccation resistance in embryos of the mosquito vectors Aedes aegypti, Anopheles aquasalis and Culex quinquefasciatus","volume":"62","author":"HC Vargas","year":"2014","journal-title":"J Insect Physiol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1016\/1044-0305(94)80016-2","article-title":"An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database","volume":"5","author":"JK Eng","year":"1994","journal-title":"J Am Soc Mass Spectrom"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s004410050915","article-title":"Secretory and internalization pathways of mosquito yolk protein precursors","volume":"290","author":"ES Snigirevskaya","year":"1997","journal-title":"Cell Tissue Res"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1186\/1756-0500-3-248","article-title":"aeGEPUCI: a database of gene expression in the dengue vector mosquito, Aedes aegypti","volume":"3","author":"SN Dissanayake","year":"2010","journal-title":"BMC Res Notes"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1126\/science.158.3809.1638","article-title":"Evolution of Structure and Function of Proteases\u2014Amino Acid Sequences of Proteolytic Enzymes Reflect Phylogenetic Relationships","volume":"158","author":"H Neurath","year":"1967","journal-title":"Science"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1006\/scdb.1999.0336","article-title":"Gene function, gene networks and the fate of duplicated genes","volume":"10","author":"SM Shimeld","year":"1999","journal-title":"Semin Cell Dev Biol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1590\/S0074-02762010000300003","article-title":"Culex quinquefasciatus vitellogenesis: morphological and biochemical aspects","volume":"105","author":"AF Cardoso","year":"2010","journal-title":"Mem Inst Oswaldo Cruz"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0118736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T13:21:08Z","timestamp":1540214468000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0118736"}},"subtitle":[],"editor":[{"given":"Pedro Lagerblad","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,2,24]]},"references-count":50,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,2,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0118736","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,24]]}}}