{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:42:42Z","timestamp":1791265362197,"version":"4.1.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T00:00:00Z","timestamp":1426464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0120325","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T18:35:34Z","timestamp":1426530934000},"page":"e0120325","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Pregnancy-Induced Amelioration of Muscular Dystrophy Phenotype in mdx Mice via Muscle Membrane Stabilization Effect of Glucocorticoid"],"prefix":"10.1371","volume":"10","author":[{"given":"Yuko","family":"Shimizu-Motohashi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Asakura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Motohashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandkishore R.","family":"Belur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Baumrucker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Asakura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,3,16]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1097\/GRF.0b013e31828f272b","article-title":"Multiple sclerosis and pregnancy","volume":"56","author":"M Houtchens","year":"2013","journal-title":"Clin Obstet Gynecol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jns.2007.10.024","article-title":"Effects of estrogen on lifespan and motor functions in female hSOD1 G93A transgenic mice","volume":"268","author":"CI Choi","year":"2008","journal-title":"J Neurol Sci"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S1474-4422(09)70272-8","article-title":"Diagnosis and management of Duchenne muscular dystrophy, part 2: implementation of multidisciplinary care","volume":"9","author":"K Bushby","year":"2010","journal-title":"Lancet Neurol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/323646a0","article-title":"Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene","volume":"323","author":"AP Monaco","year":"1986","journal-title":"Nature"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/0092-8674(87)90579-4","article-title":"Dystrophin: the protein product of the Duchenne muscular dystrophy locus","volume":"51","author":"EP Hoffman","year":"1987","journal-title":"Cell"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.biocel.2010.11.005","article-title":"Duchenne muscular dystrophy\u2014what causes the increased membrane permeability in skeletal muscle?","volume":"43","author":"DG Allen","year":"2011","journal-title":"Int J Biochem Cell Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1083\/jcb.128.3.355","article-title":"Mechanical function of dystrophin in muscle cells","volume":"128","author":"C Pasternak","year":"1995","journal-title":"J Cell Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/cge.12048","article-title":"Comparison of X-chromosome inactivation in Duchenne muscle\/myocardium-manifesting carriers, non-manifesting carriers and related daughters","volume":"84","author":"E Viggiano","year":"2013","journal-title":"Clin Genet"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1471-2350-13-73","article-title":"Genetic characterization in symptomatic female DMD carriers: lack of relationship between X-inactivation, transcriptional DMD allele balancing and phenotype","volume":"13","author":"S Brioschi","year":"2012","journal-title":"BMC Med Genet"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1002\/ana.23989","article-title":"Therapeutic advances in muscular dystrophy","volume":"74","author":"DG Leung","year":"2013","journal-title":"Ann Neurol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1002\/emmm.201302621","article-title":"VBP15, a novel anti-inflammatory and membrane-stabilizer, improves muscular dystrophy without side effects","volume":"5","author":"CR Heier","year":"2013","journal-title":"EMBO Mol Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.nmd.2004.02.011","article-title":"Prednisone reduces muscle degeneration in dystrophin-deficient Caenorhabditis elegans","volume":"14","author":"A Gaud","year":"2004","journal-title":"Neuromuscul Disord"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0960-8966(94)00042-8","article-title":"The effect of glucocorticoids on the accumulation of utrophin by cultured normal and dystrophic human skeletal muscle satellite cells","volume":"5","author":"F Pasquini","year":"1995","journal-title":"Neuromuscul Disord"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/0022-510X(91)90019-4","article-title":"Methylprednisolone increases dystrophin levels by inhibiting myotube death during myogenesis of normal human muscle in vitro","volume":"101","author":"RM Sklar","year":"1991","journal-title":"J Neurol Sci"},{"key":"ref15","first-page":"E67","article-title":"Effect of prednisone on protein metabolism in Duchenne dystrophy","volume":"268","author":"Z Rifai","year":"1995","journal-title":"Am J Physiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1007\/BF00869001","article-title":"Prednisone can protect against exercise-induced muscle damage","volume":"243","author":"SC Jacobs","year":"1996","journal-title":"J Neurol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1210\/endo-106-4-1198","article-title":"Dexamethasone effects on myoblast proliferation and differentiation","volume":"106","author":"V Guerriero Jr.","year":"1980","journal-title":"Endocrinology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1038\/sj.bjp.0702036","article-title":"Calcium influx inhibition by steroids and analogs in C2C12 skeletal muscle cells","volume":"124","author":"AC Passaquin","year":"1998","journal-title":"Br J Pharmacol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.nmd.2004.04.008","article-title":"Prednisolone decreases cellular adhesion molecules required for inflammatory cell infiltration in dystrophin-deficient skeletal muscle","volume":"14","author":"M Wehling-Henricks","year":"2004","journal-title":"Neuromuscul Disord"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1002\/path.4231","article-title":"Selective modulation through the glucocorticoid receptor ameliorates muscle pathology in mdx mice","volume":"231","author":"T Huynh","year":"2013","journal-title":"J Pathol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1212\/WNL.41.5.667","article-title":"Mononuclear cell analysis of muscle biopsies in prednisone-treated and untreated Duchenne muscular dystrophy. CIDD Study Group","volume":"41","author":"JT Kissel","year":"1991","journal-title":"Neurology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1111\/j.1476-5381.1996.tb15445.x","article-title":"Regulation of cytosolic calcium in skeletal muscle cells of the mdx mouse under conditions of stress","volume":"118","author":"WJ Leijendekker","year":"1996","journal-title":"Br J Pharmacol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1111\/j.1476-5381.1995.tb15930.x","article-title":"Modulation by prednisolone of calcium handling in skeletal muscle cells","volume":"116","author":"L Metzinger","year":"1995","journal-title":"Br J Pharmacol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1002\/1097-4598(200103)24:3<428::AID-MUS1018>3.0.CO;2-E","article-title":"Impact of prednisone on TGF-beta1 and collagen in diaphragm muscle from mdx mice","volume":"24","author":"JV Hartel","year":"2001","journal-title":"Muscle Nerve"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1096\/fj.04-2511fje","article-title":"Prednisolone-induced changes in dystrophic skeletal muscle","volume":"19","author":"I Fisher","year":"2005","journal-title":"Faseb J"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1242\/dmm.012211","article-title":"Levels of alpha7 integrin and laminin-alpha2 are increased following prednisone treatment in the mdx mouse and GRMD dog models of Duchenne muscular dystrophy","volume":"6","author":"RD Wuebbles","year":"2013","journal-title":"Dis Model Mech"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1096\/fj.04-1859fje","article-title":"Glucocorticoid treatment alleviates dystrophic myofiber pathology by activation of the calcineurin\/NF-AT pathway","volume":"18","author":"SJ St-Pierre","year":"2004","journal-title":"Faseb J"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"4145","DOI":"10.1093\/hmg\/ddq334","article-title":"Flt-1 haploinsufficiency ameliorates muscular dystrophy phenotype by developmentally increased vasculature in mdx mice","volume":"19","author":"M Verma","year":"2010","journal-title":"Hum Mol Genet"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1093\/jb\/118.5.959","article-title":"Visualization of dystrophic muscle fibers in mdx mouse by vital staining with Evans blue: evidence of apoptosis in dystrophin-deficient muscle","volume":"118","author":"R Matsuda","year":"1995","journal-title":"J Biochem"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Verma M, Asakura A. Efficient Single Muscle Fiber Isolation From Alcohol Fixed Adult Muscle Following {beta}-galactosidase Staining for Satellite Cell Detection. J Histochem Cytochem. 2010.","DOI":"10.1369\/jhc.2010.956730"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Pasut A, Jones AE, Rudnicki MA. Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. J Vis Exp. 2013: e50074.","DOI":"10.3791\/50074"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"20614","DOI":"10.1073\/pnas.1215765109","article-title":"Wnt7a treatment ameliorates muscular dystrophy","volume":"109","author":"J von Maltzahn","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"Motohashi N, Asakura Y, Asakura A. Isolation, culture, and transplantation of muscle satellite cells. J Vis Exp. 2014.","DOI":"10.3791\/50846"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1083\/jcb.145.6.1325","article-title":"alpha-Dystroglycan is a laminin receptor involved in extracellular matrix assembly on myotubes and muscle cell viability","volume":"145","author":"F Montanaro","year":"1999","journal-title":"J Cell Biol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1002\/stem.598","article-title":"MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells","volume":"29","author":"S Watanabe","year":"2011","journal-title":"Stem Cells"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"TD Schmittgen","year":"2008","journal-title":"Nat Protoc"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1002\/mus.21527","article-title":"Use of Evans blue dye to compare limb muscles in exercised young and old mdx mice","volume":"41","author":"CI Wooddell","year":"2010","journal-title":"Muscle Nerve"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1006\/clim.2000.4966","article-title":"Helper (CD4(+)) and cytotoxic (CD8(+)) T cells promote the pathology of dystrophin-deficient muscle","volume":"98","author":"MJ Spencer","year":"2001","journal-title":"Clin Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/0092-8674(88)90463-1","article-title":"Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy","volume":"52","author":"C Webster","year":"1988","journal-title":"Cell"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nrg3460","article-title":"Therapy for Duchenne muscular dystrophy: renewed optimism from genetic approaches","volume":"14","author":"RJ Fairclough","year":"2013","journal-title":"Nat Rev Genet"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.yfrne.2009.03.007","article-title":"Effects of steroid hormones on neurogenesis in the hippocampus of the adult female rodent during the estrous cycle, pregnancy, lactation and aging","volume":"30","author":"JL Pawluski","year":"2009","journal-title":"Front Neuroendocrinol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0306-4530(88)90005-4","article-title":"Hormonal basis during pregnancy for the onset of maternal behavior in the rat","volume":"13","author":"JS Rosenblatt","year":"1988","journal-title":"Psychoneuroendocrinology"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1152\/ajplegacy.1969.216.3.655","article-title":"Influence of cycling, pregnancy, labor, and suckling on corticosterone-ACTH levels","volume":"216","author":"JL Voogt","year":"1969","journal-title":"Am J Physiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1210\/endo.136.2.7835284","article-title":"The hypothalamic-pituitary-adrenal axis in rat pregnancy and lactation: circadian variation and interrelationship of plasma adrenocorticotropin and corticosterone","volume":"136","author":"HC Atkinson","year":"1995","journal-title":"Endocrinology"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/349069a0","article-title":"Decreased osmotic stability of dystrophin-less muscle cells from the mdx mouse","volume":"349","author":"A Menke","year":"1991","journal-title":"Nature"},{"issue":"2","key":"ref46","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1242\/jcs.108.2.727","article-title":"Extent of shock-induced membrane leakage in human and mouse myotubes depends on dystrophin","volume":"108","author":"A Menke","year":"1995","journal-title":"J Cell Sci"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1096\/fj.13-237958","article-title":"Endogenous glucocorticoid receptor signaling drives rhythmic changes in human T-cell subset numbers and the expression of the chemokine receptor CXCR4","volume":"28","author":"L Besedovsky","year":"2014","journal-title":"Faseb J"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1242\/jcs.02952","article-title":"Severe muscular dystrophy in mice that lack dystrophin and alpha7 integrin","volume":"119","author":"JE Rooney","year":"2006","journal-title":"J Cell Sci"},{"key":"ref49","first-page":"e5035add5038caff5034","article-title":"The C2A domain in dysferlin is important for association with MG53 (TRIM72)","volume":"4","author":"C Matsuda","year":"2012","journal-title":"PLoS Curr"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1172\/JCI43844","article-title":"Mitigation of muscular dystrophy in mice by SERCA overexpression in skeletal muscle","volume":"121","author":"SA Goonasekera","year":"2011","journal-title":"J Clin Invest"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"35202","DOI":"10.1074\/jbc.M606406200","article-title":"Requirement for annexin A1 in plasma membrane repair","volume":"281","author":"AK McNeil","year":"2006","journal-title":"J Biol Chem"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1038\/nri2470","article-title":"Annexin A1 and glucocorticoids as effectors of the resolution of inflammation","volume":"9","author":"M Perretti","year":"2009","journal-title":"Nat Rev Immunol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1126\/science.1076647","article-title":"Pregnancy-stimulated neurogenesis in the adult female forebrain mediated by prolactin","volume":"299","author":"T Shingo","year":"2003","journal-title":"Science"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/nrn1909","article-title":"Why sex matters for neuroscience","volume":"7","author":"L Cahill","year":"2006","journal-title":"Nat Rev Neurosci"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.physbeh.2008.09.004","article-title":"Reproductive experience alters corticosterone and CBG levels in the rat dam","volume":"96","author":"JL Pawluski","year":"2009","journal-title":"Physiol Behav"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.biopsych.2006.10.033","article-title":"Neurogenesis and helplessness are mediated by controllability in males but not in females","volume":"62","author":"TJ Shors","year":"2007","journal-title":"Biol Psychiatry"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.cmet.2011.08.005","article-title":"Estrogen receptors and the metabolic network","volume":"14","author":"RP Barros","year":"2011","journal-title":"Cell Metab"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.ajpath.2012.10.018","article-title":"The anticancer drug tamoxifen counteracts the pathology in a mouse model of duchenne muscular dystrophy","volume":"182","author":"OM Dorchies","year":"2013","journal-title":"Am J Pathol"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"E1173","DOI":"10.1152\/ajpendo.00102.2006","article-title":"Estrogen potentiates adrenocortical responses to stress in female rats","volume":"292","author":"HF Figueiredo","year":"2007","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"e622","DOI":"10.1161\/CIRCULATIONAHA.113.001851","article-title":"Cardiology patient page: peripartum cardiomyopathy","volume":"127","author":"MM Givertz","year":"2013","journal-title":"Circulation"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/2044-5040-3-9","article-title":"Vascular-targeted therapies for Duchenne muscular dystrophy","volume":"3","author":"JP Ennen","year":"2013","journal-title":"Skelet Muscle"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"50","DOI":"10.3389\/fphys.2014.00050","article-title":"Angiogenesis as a novel therapeutic strategy for Duchenne muscular dystrophy through decreased ischemia and increased satellite cells","volume":"5","author":"Y Shimizu-Motohashi","year":"2014","journal-title":"Front Physiol"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"e62662","DOI":"10.1371\/journal.pone.0062662","article-title":"Distant mesenchymal progenitors contribute to skin wound healing and produce collagen: evidence from a murine fetal microchimerism model","volume":"8","author":"E Seppanen","year":"2013","journal-title":"PLoS One"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1002\/jcp.24032","article-title":"Annexin A1: novel roles in skeletal muscle biology","volume":"227","author":"V Bizzarro","year":"2012","journal-title":"J Cell Physiol"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"6004","DOI":"10.1073\/pnas.1324242111","article-title":"Annexin A6 modifies muscular dystrophy by mediating sarcolemmal repair","volume":"111","author":"KA Swaggart","year":"2014","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0120325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T02:02:26Z","timestamp":1651543346000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0120325"}},"subtitle":[],"editor":[{"given":"Paul","family":"McNeil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,3,16]]},"references-count":65,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2015,3,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0120325","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,16]]}}}