{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T16:22:34Z","timestamp":1774455754933,"version":"3.50.1"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2015,3,19]],"date-time":"2015-03-19T00:00:00Z","timestamp":1426723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0120603","type":"journal-article","created":{"date-parts":[[2015,3,19]],"date-time":"2015-03-19T18:35:21Z","timestamp":1426790121000},"page":"e0120603","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Ligand Binding to the FA3-FA4 Cleft Inhibits the Esterase-Like Activity of Human Serum Albumin"],"prefix":"10.1371","volume":"10","author":[{"given":"Paolo","family":"Ascenzi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loris","family":"Leboffe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"di Masi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana","family":"Trezza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella","family":"Fanali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda","family":"Gioia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Coletta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Fasano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,3,19]]},"reference":[{"key":"ref1","article-title":"All about Albumin: Biochemistry, Genetics and Medical Applications","author":"T Peters Jr","year":"1996"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.mam.2011.12.002","article-title":"Human serum albumin: from bench to bedside","volume":"33","author":"G Fanali","year":"2012","journal-title":"Mol Aspects Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"5535","DOI":"10.1016\/j.bbagen.2013.03.015","article-title":"Molecular and practical aspects of the enzymatic properties of human serum albumin and of albumin-ligand complexes","volume":"1830","author":"U Kragh-Hansen","year":"2013","journal-title":"Biochim Biophys Acta"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0014-5793(76)80496-6","article-title":"Lysine residue 199 of human serum albumin is modified by acetylsalicyclic acid","volume":"66","author":"JE Walker","year":"1976","journal-title":"FEBS Lett"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1042\/bj3490813","article-title":"Role of Arg-410 and Tyr-411 in human serum albumin for ligand binding and esterase-like activity","volume":"349","author":"H Watanabe","year":"2000","journal-title":"Biochem J"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1021\/jm000340v","article-title":"Molecular dynamics study of the IIA binding site in human serum albumin: influence of the protonation state of Lys195 and Lys199","volume":"44","author":"N D\u00edaz","year":"2001","journal-title":"J Med Chem"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1248\/bpb.25.695","article-title":"Practical aspects of the ligand-binding and enzymatic properties of human serum albumin","volume":"25","author":"U Kragh-Hansen","year":"2002","journal-title":"Biol Pharm Bull"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jmb.2005.07.075","article-title":"Structural basis of the drug-binding specificity of human serum albumin","volume":"353","author":"J Ghuman","year":"2005","journal-title":"J Mol Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1023\/B:PHAM.0000016241.84630.06","article-title":"Esterase-like activity of serum albumin: characterization of its structural chemistry using p-nitrophenyl esters as substrates","volume":"21","author":"Y Sakurai","year":"2004","journal-title":"Pharm Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1002\/jps.2600720416","article-title":"Esterase-like activity of human serum albumin II: reaction with N-trans-cinnamoylimidazoles","volume":"72","author":"N Ohta","year":"1983","journal-title":"J Pharm Sci"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.1248\/cpb.40.2169","article-title":"Esterase-like activity of human serum albumin. VIII. Reaction with amino acid p-nitrophenyl esters","volume":"40","author":"Y Kurono","year":"1992","journal-title":"Chem Pharm Bull"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.bbrc.2012.04.111","article-title":"Pseudo-enzymatic hydrolysis of 4-nitrophenyl myristate by human serum albumin","volume":"422","author":"P Ascenzi","year":"2012","journal-title":"Biochem Biophys Res Commun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.bbrc.2012.06.131","article-title":"Pseudo-enzymatic hydrolysis of 4-nitrophenyl acetate by human serum albumin: pH-dependence of rates of individual steps","volume":"424","author":"P Ascenzi","year":"2012","journal-title":"Biochem Biophys Res Commun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"22582","DOI":"10.1074\/jbc.M802555200","article-title":"Pseudo-esterase activity of human albumin: slow turnover on tyrosine 411 and stable acetylation of 82 residues including 59 lysines","volume":"283","author":"O Lockridge","year":"2008","journal-title":"J Biol Chem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"4989","DOI":"10.1021\/bi00693a031","article-title":"Acetylation of human serum albumin by <italic>p<\/italic>-nitrophenyl acetate","volume":"14","author":"GE Means","year":"1975","journal-title":"Biochemistry"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1111\/j.1432-1033.1971.tb19691.x","article-title":"The pH-dependence of rates of individual steps in ficin catalysis","volume":"24","author":"MR Hollaway","year":"1971","journal-title":"Eur J Biochem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"12449","DOI":"10.1016\/S0021-9258(18)43294-2","article-title":"The mechanism of trypsin catalysis at low pH: proposal for a structural model","volume":"256","author":"E Antonini","year":"1981","journal-title":"J Biol Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"5907","DOI":"10.1021\/ja01531a017","article-title":"Multiple intermediates in steady state enzyme kinetics. I. The mechanism involving a single substrate and product","volume":"81","author":"L Peller","year":"1959","journal-title":"J Am Chem Soc"},{"key":"ref19","first-page":"134","article-title":"Enzyme competitive inhibition. Graphical determination of K<sub>i<\/sub> and presentation of data in comparative studies","volume":"15","author":"P Ascenzi","year":"1987","journal-title":"Biochem Mol Biol Edu"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0031-6997(25)06849-8","article-title":"Molecular aspects of ligand binding to serum albumin","volume":"33","author":"U Kragh-Hansen","year":"1981","journal-title":"Pharmacol Rev"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1016\/S0731-7085(96)01956-5","article-title":"A quantitative circular dichroic investigation of the binding of the enantiomers of ibuprofen and naproxen to human serum albumin","volume":"15","author":"VK Cheruvallath","year":"1997","journal-title":"J Pharm Biomed Anal"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1023\/A:1011014629551","article-title":"Interaction mechanism between IS, a typical uremic toxin bound to site II, and ligands bound to site I of human serum albumin","volume":"18","author":"T Sakai","year":"2001","journal-title":"Pharm Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"12675","DOI":"10.1021\/bi035941d","article-title":"Binding of volatile anesthetics to serum albumin: measurements of enthalpy and solvent contributions","volume":"43","author":"AH Sawas","year":"2004","journal-title":"Biochemistry"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1211\/jpp.58.11.0007","article-title":"In-vitro study on the competitive binding of diflunisal and uraemic toxins to serum albumin and human plasma using a potentiometric ion-probe technique","volume":"58","author":"A Davilas","year":"2006","journal-title":"J Pharm Pharmacol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1002\/iub.466","article-title":"Binding of \u03b49-tetrahydrocannabinol and diazepam to human serum albumin","volume":"63","author":"G Fanali","year":"2011","journal-title":"IUBMB Life"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"416","DOI":"10.3390\/toxins6020416","article-title":"Binding affinity and capacity for the uremic toxin indoxyl sulfate","volume":"6","author":"E Devine","year":"2014","journal-title":"Toxins"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"E653","DOI":"10.1073\/pnas.1221050110","article-title":"Intrinsic evolutionary constraints on protease structure, enzyme acylation, and the identity of the catalytic triad","volume":"110","author":"AR Buller","year":"2013","journal-title":"Proc Natl Acad Sci U. S. A"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.jsb.2006.08.015","article-title":"Effect of human serum albumin on drug metabolism: structural evidence of esterase activity of human serum albumin","volume":"157","author":"F Yang","year":"2007","journal-title":"J Struct Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.bcp.2009.10.007","article-title":"Reaction of human albumin with aspirin <italic>in vitro<\/italic>: mass spectrometric identification of acetylated lysines 199, 402, 519, and 545","volume":"79","author":"MS Liyasova","year":"2010","journal-title":"Biochem Pharmacol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"38731","DOI":"10.1074\/jbc.M005460200","article-title":"Binding of the general anesthetics propofol and halothane to human serum albumin: high resolution crystal structures","volume":"275","author":"AA Bhattacharya","year":"2000","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"342","DOI":"10.2133\/dmpk.24.342","article-title":"Lessons from the crystallographic analysis of small molecule binding to human serum albumin","volume":"24","author":"S Curry","year":"2009","journal-title":"Drug Metab Pharmacokinet"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1093\/protein\/12.6.439","article-title":"Crystal structure of human serum albumin at 2.5 \u00c5 resolution","volume":"12","author":"S Sugio","year":"1999","journal-title":"Protein Eng"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2918","DOI":"10.1016\/S0021-9258(18)93359-4","article-title":"The binding of carbon monoxide by human hemoglobin: proof of validity of the spectrophotometric method and direct determination of the equilibrium","volume":"243","author":"E Antonini","year":"1968","journal-title":"J Biol Chem"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF00421784","article-title":"The concentrations of diazepam and its metabolites in the plasma after an acute and chronic administration","volume":"36","author":"J Kanto","year":"1974","journal-title":"Psychopharmacologia"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1002\/cpt1978234371","article-title":"Relationship between diazepam dose, plasma level, age, and central nervous system depression","volume":"23","author":"MM Reidenberg","year":"1978","journal-title":"Clin Pharmacol Ther"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1365-2125.1978.tb01684.x","article-title":"Plasma concentrations of diazepam and desmethyldiazepam during chronic diazepam therapy","volume":"6","author":"DM Rutherford","year":"1978","journal-title":"Br J Clin Pharmacol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"31S","DOI":"10.1111\/j.1365-2125.1977.tb04511.x","article-title":"Diflunisal: a review of pharmacokinetic and pharmacodynamic properties, drug interactions, and special tolerability studies in humans","volume":"1","author":"KF Tempero","year":"1977","journal-title":"Br J Clin Pharmacol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1002\/j.1875-9114.1985.tb03440.x","article-title":"Steady-state disposition of diflunisal: once- versus twice-daily administration","volume":"5","author":"P Mojaverian","year":"1985","journal-title":"Pharmacotherapy"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1177\/030006058501300110","article-title":"Ibuprofen: plasma concentrations in man","volume":"13","author":"GM Janssen","year":"1985","journal-title":"J Int Med Res"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/clpt.1986.129","article-title":"The correlation between blood levels of ibuprofen and clinical analgesic response","volume":"40","author":"EM Laska","year":"1986","journal-title":"Clin Pharmacol Ther"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"308","DOI":"10.2165\/00003088-198917050-00002","article-title":"Pharmacokinetic implications for the clinical use of propofol","volume":"17","author":"J Kanto","year":"1989","journal-title":"Clin Pharmacokinet"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1034\/j.1399-6576.2002.460814.x","article-title":"Target-controlled infusion of propofol induction with or without plasma concentration constraint in high-risk adult patients undergoing cardiac surgery","volume":"46","author":"V Macquaire","year":"2002","journal-title":"Acta Anaesthesiol Scand"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1111\/bcpt.12277","article-title":"Pharmacogenetics, plasma concentrations, clinical signs and EEG during propofol treatment","volume":"115","author":"MS Khan","year":"2014","journal-title":"Basic Clin Pharmacol Toxicol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1093\/clinchem\/32.6.938","article-title":"Determination of indoxyl sulfate in plasma of patients with renal failure by use of ion-pairing liquid chromatography","volume":"32","author":"LA Stanfel","year":"1986","journal-title":"Clin Chem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1002\/iub.1208","article-title":"Functional role of transient conformations: rediscovering \u201cchronosteric effects\u201d thirty years later","volume":"65","author":"P Ascenzi","year":"2013","journal-title":"IUBMB Life"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014a visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0120603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T04:26:17Z","timestamp":1747801577000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0120603"}},"subtitle":[],"editor":[{"given":"Eugene A.","family":"Permyakov","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,3,19]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2015,3,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0120603","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,19]]}}}