{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T05:44:17Z","timestamp":1762407857709},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0121097","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T19:14:45Z","timestamp":1427915685000},"page":"e0121097","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Site-Specific Cassette Exchange Systems in the Aedes aegypti Mosquito and the Plutella xylostella Moth"],"prefix":"10.1371","volume":"10","author":[{"given":"Roya Elaine","family":"Haghighat-Khah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Scaife","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"St John","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly Jean","family":"Matzen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke","family":"Alphey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,4,1]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1089\/vbz.2007.0273","article-title":"Guidance for contained field trials of vector mosquitoes engineered to contain a gene drive system: recommendations of a scientific working group","volume":"8","author":"M Benedict","year":"2008","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1128\/JVI.47.2.287-300.1983","article-title":"Acquisition of host-cell DNA-sequences by baculoviruses: relationship between host DNA insertions and FP mutants of Autographa californica and Galleria mellonella nuclear polyhedrosis viruses","volume":"47","author":"MJ Fraser","year":"1983","journal-title":"J Virol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0109690","article-title":"Fitness Cost Implications of PhiC31-Mediated Site-Specific Integrations in Target-Site Strains of the Mexican Fruit Fly, Anastrepha ludens (Diptera : Tephritidae)","volume":"9","author":"S Meza","year":"2014","journal-title":"PLoS One"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1534\/genetics.166.4.1775","article-title":"Construction of transgenic Drosophila by using the site-specific integrase from phage \u03a6C31","volume":"166","author":"AC Groth","year":"2004","journal-title":"Genetics"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"5505","DOI":"10.1073\/pnas.95.10.5505","article-title":"In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase\/invertase family","volume":"95","author":"HM Thorpe","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1365-2583.2006.00615.x","article-title":"High efficiency site-specific genetic engineering of the mosquito genome","volume":"15","author":"DD Nimmo","year":"2006","journal-title":"Insect Mol Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"18171","DOI":"10.1073\/pnas.0907264106","article-title":"Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata","volume":"106","author":"MF Schetelig","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"e788","DOI":"10.1371\/journal.pntd.0000788","article-title":"piggybac- and PhiC3-mediated genetic transformation of the Asian tiger mosquito, Aedes albopictus (Skuse)","volume":"4","author":"GMC Labb\u00e9","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e14587","DOI":"10.1371\/journal.pone.0014587","article-title":"Site-specific integration and expression of an anti-malarial gene in transgenic Anopheles gambiae significantly reduces Plasmodium infections","volume":"6","author":"JM Meredith","year":"2011","journal-title":"PLoS One"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s13355-013-0182-6","article-title":"phiC31-integrase-mediated, site-specific integration of transgenes in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)","volume":"48","author":"N Yonemura","year":"2013","journal-title":"Appl Entomol Zool"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"5995","DOI":"10.1073\/pnas.090527097","article-title":"A phage integrase directs efficient site-specific integration in human cells","volume":"97","author":"AC Groth","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e45353","DOI":"10.1371\/journal.pone.0045353","article-title":"phiC31 integrase-mediated site-specific recombination in barley","volume":"7","author":"E Kapusi","year":"2012","journal-title":"PLoS One"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1038\/nbt753","article-title":"Site-specific genomic integration produces therapeutic Factor IX levels in mice","volume":"20","author":"EC Olivares","year":"2002","journal-title":"Nat Biotechnol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1002\/dvdy.23989","article-title":"Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system","volume":"242","author":"C Mosimann","year":"2014","journal-title":"Dev Dyn"},{"key":"ref15","article-title":"Ethical, legal and social issues of genetically modified disease vectors in public health","author":"D Macer","year":"2003","journal-title":"World Health Organisation"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"298","DOI":"10.2307\/1937535","article-title":"The planned introduction of genetically engineered organisms: ecological considerations and recommendations","volume":"70","author":"JM Tiedje","year":"1989","journal-title":"Ecology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0169-4758(95)80086-7","article-title":"Impact of risk analyses on pest-management programs employing transgenic arthropods","volume":"11","author":"MA Hoy","year":"1995","journal-title":"Parasitol Today"},{"key":"ref18","unstructured":"Nuffield Council on Bioethics (1999) The ethics of clinical research in developing countries. 2011. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.nuffieldbioethics.org\/sites\/default\/files\/files\/Clinical\" xlink:type=\"simple\">http:\/\/www.nuffieldbioethics.org\/sites\/default\/files\/files\/Clinical<\/ext-link> research in developing countries Discussion Paper.pdf."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/S0958-1669(00)00248-2","article-title":"Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes","volume":"12","author":"A Baer","year":"2001","journal-title":"Curr Opin Biotechnol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jmb.2011.01.004","article-title":"Recombinase-mediated cassette exchange (RMCE): traditional concepts and current challenges","volume":"407","author":"S Turan","year":"2011","journal-title":"J Mol Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"5166","DOI":"10.1073\/pnas.85.14.5166","article-title":"Site-specific DNA recombination in mammalian cells by the cre recombinase of bacteriophage P1","volume":"85","author":"B Sauer","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref22","first-page":"4223","article-title":"The FLP protein of the yeast 2-eum plasmid : Expression of a eukaryotic genetic recombination system in Escherichia coli Biochemistry","volume":"80","author":"MM Cox","year":"1983","journal-title":"The FLP protein of the yeast 2-eum plasmid\u202f: Expression of a eukaryotic genetic recombination system in Escherichia coli Biochemistry"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1093\/nar\/gnf114","article-title":"Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases","volume":"30","author":"M Lauth","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1007\/s10126-007-9000-x","article-title":"Site-directed gene integration in transgenic zebrafish mediated by cre recombinase using a combination of mutant lox sites","volume":"9","author":"W Liu","year":"2007","journal-title":"Mar Biotechnol (NY)"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e62","DOI":"10.1093\/nar\/gks027","article-title":"Flp and Cre expressed from Flp-2A-Cre and Flp-IRES-Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange","volume":"40","author":"RP Anderson","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1104\/pp.107.108092","article-title":"Stable recombinase-mediated cassette exchange in Arabidopsis using Agrobacterium tumefaciens","volume":"145","author":"JD Louwerse","year":"2007","journal-title":"Plant Physiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-1119(98)00130-9","article-title":"A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants","volume":"212","author":"T Hoang","year":"1998","journal-title":"Gene"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.2144\/02335rr01","article-title":"Broad-Host-Range cre-lox System for Antibiotic Marker Recycling in Gram-Negative Bacteria","volume":"1067","author":"CJ Marx","year":"2002","journal-title":"Biotechniques"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/nmeth775","article-title":"Site-specific transgenesis by Cre-mediated recombination in Drosophila","volume":"2","author":"A Oberstein","year":"2005","journal-title":"Nat Methods"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"12483","DOI":"10.1073\/pnas.0504305102","article-title":"Site-specific genomic targeting in Drosophila","volume":"102","author":"C Horn","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e40150","DOI":"10.1371\/journal.pone.0040150","article-title":"FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori","volume":"7","author":"D-P Long","year":"2012","journal-title":"PLoS One"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1534\/g3.112.005488","article-title":"A Functional Comparison of the 3xP3 Promoter by Recombinase-Mediated Cassette Exchange in Drosophila and a Tephritid Fly, Anastrepha suspensa","volume":"3","author":"MF Schetelig","year":"2013","journal-title":"G3 (Bethesda)"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1534\/genetics.106.056945","article-title":"Site-specific transformation of Drosophila via \u03a6C31 integrase-mediated cassette exchange","volume":"173","author":"JR Bateman","year":"2006","journal-title":"Genetics"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.ibmb.2013.08.001","article-title":"In vivo site-specific integration of transgene in silkworm via PhiC31 integrase-mediated cassette exchange","volume":"43","author":"D Long","year":"2013","journal-title":"Insect Biochem Mol Biol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"12746","DOI":"10.1021\/bi00209a003","article-title":"Use of Mutated FLP Recognition Target (FRT) Sites for the Exchange of Expression Cassettes at Defined Chromosomal Loci","volume":"33","author":"T Schlake","year":"1994","journal-title":"Biochemistry"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1006\/viro.1998.9415","article-title":"Characterization of the acidic domain of the IE1 regulatory protein from Orgyia pseudotsugata multicapsid nucleopolyhedrovirus","volume":"252","author":"IJ Forsythe","year":"1998","journal-title":"Virology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0378-1119(96)00756-1","article-title":"Baculovirus immediate-early promoter-mediated expression of the Zeocin?? resistance gene for use as a dominant selectable marker in Dipteran and Lepidopteran insect cell lines","volume":"188","author":"TA Pfeifer","year":"1997","journal-title":"Gene"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1101\/SQB.1984.049.01.089","article-title":"In vitro systems for genetic recombination of the DNAs of bacteriophage T7 and yeast 2-micron circle","volume":"49","author":"P Sadowski","year":"1984","journal-title":"Cold Spring Harb Symp Quant Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/0092-8674(93)90644-6","article-title":"Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting","volume":"73","author":"H Gu","year":"1993","journal-title":"Cell"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/0092-8674(88)90216-4","article-title":"A protein component of Drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicases","volume":"55","author":"B Hay","year":"1988","journal-title":"Cell"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2915.1989.tb00467.x","article-title":"Genetic transformation of the mosquito Aedes aegypti by micro-injection of DNA","volume":"3","author":"AC Morris","year":"1989","journal-title":"Med Vet Entomol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1111\/j.1365-2583.2012.01146.x","article-title":"Germline transformation of the diamondback moth, Plutella xylostella L., using the piggyBac transposable element","volume":"21","author":"S Martins","year":"2012","journal-title":"Insect Mol Biol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0092-8674(83)90133-2","article-title":"Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome","volume":"34","author":"K O\u2019Hare","year":"1983","journal-title":"Cell"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1006\/viro.1995.1422","article-title":"Assay for movement of Lepidopteran transposon IFP2 in insect cells using a baculovirus genome as a target DNA","volume":"211","author":"MJ Fraser","year":"1995","journal-title":"Virology"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3926","DOI":"10.1128\/MCB.21.12.3926-3934.2001","article-title":"Site-specific genomic integration in mammalian cells mediated by phage \u03a6C31 integrase","volume":"21","author":"B Thyagarajan","year":"2001","journal-title":"Mol Cell Biol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jmb.2005.11.098","article-title":"Integration specificity of phage \u03a6 C31 integrase in the human genome","volume":"357","author":"TW Chalberg","year":"2006","journal-title":"J Mol Biol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"e147","DOI":"10.1093\/nar\/gng148","article-title":"High efficiency, site-specific excision of a marker gene by the phage P1 cre-loxP system in the yellow fever mosquito, Aedes aegypti","volume":"31","author":"N Jasinskiene","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1038\/nbt1002","article-title":"Post-integration stabilization of a transposon vector by terminal sequence deletion in Drosophila melanogaster","volume":"22","author":"AM Handler","year":"2004","journal-title":"Nat Biotechnol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1038\/nbt1221","article-title":"Transposon-free insertions for insect genetic engineering","volume":"24","author":"TH Dafa\u2019alla","year":"2006","journal-title":"Nat Biotech"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"e26422","DOI":"10.1371\/journal.pone.0026422","article-title":"A new powerful method for site-specific transgene stabilization based on chromosomal double-strand break repair","volume":"6","author":"A Tkachuk","year":"2011","journal-title":"PLoS One"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s10709-010-9494-4","article-title":"Recombination technologies for enhanced transgene stability in bioengineered insects","volume":"139","author":"MF Schetelig","year":"2011","journal-title":"Genetica"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/nmeth0805-580","article-title":"Insect transgenesis by site-specific","volume":"2","author":"EA Wimmer","year":"2005","journal-title":"Nat Methods"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"16339","DOI":"10.1073\/pnas.1110628108","article-title":"piggyBac transposon remobilization and enhancer detection in Anopheles mosquitoes","volume":"108","author":"DA O\u2019Brochta","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.1093\/nar\/30.11.2299","article-title":"Enhanced efficiency through nuclear localization signal fusion on phage \u03a6C31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells","volume":"30","author":"S Andreas","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"3312","DOI":"10.1073\/pnas.0611511104","article-title":"An optimized transgenesis system for Drosophila using germ-line-specific \u03a6C31 integrases","volume":"104","author":"J Bischof","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"e59264","DOI":"10.1371\/journal.pone.0059264","article-title":"Next-generation site-directed transgenesis in the malaria vector mosquito Anopheles gambiae: self-docking strains expressing germline-specific phiC31 integrase","volume":"8","author":"JM Meredith","year":"2013","journal-title":"PLoS One"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1093\/genetics\/144.2.715","article-title":"Transgene coplacement and high efficiency site-specific recombination with the Cre\/loxP system in Drosophila","volume":"144","author":"ML Siegal","year":"1996","journal-title":"Genetics"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/0092-8674(82)90456-1","article-title":"Promoter occlusion: transcription through a promoter may inhibit its activity","volume":"29","author":"S Adhya","year":"1982","journal-title":"Cell"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"9780","DOI":"10.1074\/jbc.M108661200","article-title":"Sp3 represses gene expression via the titration of promoter-specific transcription factors","volume":"277","author":"SB Kennett","year":"2002","journal-title":"J Biol Chem"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.molcel.2004.05.010","article-title":"Transcriptional interference between convergent promoters caused by elongation over the promoter","volume":"14","author":"BP Callen","year":"2004","journal-title":"Mol Cell"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"2598","DOI":"10.1128\/MCB.01953-07","article-title":"Chromatin Structure and Expression of a Gene Transcribed by RNA Polymerase III Are Independent of H2A.Z Deposition","volume":"28","author":"AG Arimbasseri","year":"2008","journal-title":"Mollecular Cell Biol"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.tibtech.2013.04.004","article-title":"ZFN, TALEN, and CRISPR\/Cas-based methods for genome engineering","volume":"31","author":"T Gaj","year":"2013","journal-title":"Trends Biotechnol"},{"key":"ref63","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1404996111","article-title":"Site-specific genetic engineering of the Anopheles gambiae Y chromosome","volume":"111","author":"F Bernardini","year":"2014","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0121097","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T03:05:55Z","timestamp":1651633555000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0121097"}},"subtitle":[],"editor":[{"given":"Immo A.","family":"Hansen","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,4,1]]},"references-count":63,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,4,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0121097","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,1]]}}}