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Influenza (Seasonal). World Health Organization: 1\u20133. 2014. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/mediacentre\/factsheets\/fs211\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/mediacentre\/factsheets\/fs211\/en\/<\/ext-link>. Accessed 2014 March 11."},{"key":"ref2","first-page":"1647","article-title":"Fileds Virology","volume":"Vol. 2","author":"P Palese","year":"2007"},{"key":"ref3","unstructured":"World Health Organization. Cumulative number of confirmed human cases for avian influenza A(H5N1) reported to WHO, 2003\u20132013. World Health Organization: 1\u20132. 2013. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/influenza\/human_animal_interface\/EN_GIP_20130201CumulativeNumberH5N1cases.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/influenza\/human_animal_interface\/EN_GIP_20130201CumulativeNumberH5N1cases.pdf<\/ext-link>. Accessed 2014 March 18."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.antiviral.2013.03.014","article-title":"Influenza virus resistance to neuraminidase inhibitors","volume":"98","author":"M Samson","year":"2013","journal-title":"Antiviral Res"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/S1473-3099(11)70318-8","article-title":"Antiviral resistance during the 2009 influenza A H1N1 pandemic: public health, laboratory, and clinical perspectives","volume":"12","author":"AC Hurt","year":"2012","journal-title":"Lancet Infect Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.tibtech.2014.01.001","article-title":"Avian influenza vaccines against H5N1 \u201cbird flu.\u201d","volume":"32","author":"C Li","year":"2014","journal-title":"Trends Biotechnol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1093\/jac\/46.2.181","article-title":"Evaluation of (1<italic>R<\/italic>,2<italic>R<\/italic>)-1-(5\u2019-methylfur-3\u2019-yl)propane-1,2,3-triol, a sphydrofuran derivative isolated from a Streptomyces species, as an anti-herpesvirus drug","volume":"46","author":"K Hayashi","year":"2000","journal-title":"J Antimicrob Chemother"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"4139","DOI":"10.1128\/JCM.43.8.4139-4146.2005","article-title":"Enhanced expression of an alpha2,6-linked sialic acid on MDCK cells improves isolation of human influenza viruses and evaluation of their sensitivity to a neuraminidase inhibitor","volume":"43","author":"S Hatakeyama","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.jviromet.2011.11.022","article-title":"A quantitative comet infection assay for influenza virus","volume":"179","author":"SM Lindsay","year":"2012","journal-title":"J Virol Methods"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1007\/s007050170002","article-title":"Universal primer set for the full-length amplification of all influenza A viruses","volume":"146","author":"E Hoffmann","year":"2001","journal-title":"Arch Virol"},{"key":"ref11","unstructured":"World Health Organization. Sequencing primers and protocol. World Health Organization. 2009. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/GenomePrimers_20090512.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/GenomePrimers_20090512.pdf<\/ext-link>. Accessed 2014 May 15."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"CA Schneider","year":"2012","journal-title":"Nature Methods"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1093\/nar\/30.9.e36","article-title":"Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR","volume":"30","author":"MW Pfaffl","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1099\/vir.0.020370-0","article-title":"High yields of influenza A virus in Madin-Darby canine kidney cells are promoted by an insufficient interferon-induced antiviral state","volume":"91","author":"C Seitz","year":"2010","journal-title":"J Gen Virol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"8210","DOI":"10.1016\/j.vaccine.2010.07.076","article-title":"Differential activation of host cell signalling pathways through infection with two variants of influenza A\/Puerto Rico\/8\/34 (H1N1) in MDCK cells","volume":"28","author":"B Heynisch","year":"2010","journal-title":"Vaccine"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.1099\/vir.0.2008\/004606-0","article-title":"The multifunctional NS1 protein of influenza A viruses","volume":"89","author":"BG Hale","year":"2008","journal-title":"J Gen Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1111\/j.1462-5822.2009.01309.x","article-title":"A new player in a deadly game: influenza viruses and the PI3K\/Akt signalling pathway","volume":"11","author":"C Ehrhardt","year":"2009","journal-title":"Cell Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1073\/pnas.0910715107","article-title":"Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein","volume":"107","author":"BG Hale","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1099\/vir.0.82419-0","article-title":"Influenza A virus NS1 protein activates the phosphatidylinositol 3-kinase (PI3K)\/Akt pathway by direct interaction with the p85 subunit of PI3K","volume":"88","author":"Y-K Shin","year":"2007","journal-title":"J Gen Virol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.virol.2009.10.004","article-title":"Loss of function of the influenza A virus NS1 protein promotes apoptosis but this is not due to a failure to activate phosphatidylinositol 3-kinase (PI3K)","volume":"396","author":"D Jackson","year":"2010","journal-title":"Virology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"12730","DOI":"10.1128\/JVI.01427-07","article-title":"SH3 binding motif 1 in influenza A virus NS1 protein is essential for PI3K\/Akt signaling pathway activation","volume":"81","author":"Y-K Shin","year":"2007","journal-title":"J Virol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.virusres.2006.09.013","article-title":"Nuclear traffic of influenza virus proteins and ribonucleoprotein complexes","volume":"124","author":"S Boulo","year":"2007","journal-title":"Virus Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1093\/emboj\/cdg279","article-title":"Caspase 3 activation is essential for efficient influenza virus propagation","volume":"22","author":"WJ Wurzer","year":"2003","journal-title":"EMBO J"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"8233","DOI":"10.1128\/JVI.02672-08","article-title":"Lack of Bax Prevents Influenza A Virus-Induced Apoptosis and Causes Diminished Viral Replication","volume":"83","author":"JE McLean","year":"2009","journal-title":"J Virol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1128\/JVI.02017-12","article-title":"Influenza virus induces apoptosis via BAD-mediated mitochondrial dysregulation","volume":"87","author":"AT Tran","year":"2013","journal-title":"J Virol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e43031","DOI":"10.1371\/journal.pone.0043031","article-title":"Critical role of an antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity","volume":"7","author":"K Onomoto","year":"2012","journal-title":"PLoS ONE"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.cell.2010.01.020","article-title":"RIG-I detects viral genomic RNA during negative-strand RNA virus infection","volume":"140","author":"J 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