{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:55:00Z","timestamp":1791212100447,"version":"4.1.0"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2015,4,23]],"date-time":"2015-04-23T00:00:00Z","timestamp":1429747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0124459","type":"journal-article","created":{"date-parts":[[2015,4,23]],"date-time":"2015-04-23T20:24:19Z","timestamp":1429820659000},"page":"e0124459","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":102,"title":["Synthesis of Morphinan Alkaloids in Saccharomyces cerevisiae"],"prefix":"10.1371","volume":"10","author":[{"given":"Elena","family":"Fossati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Narcross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Ekins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Falgueyret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent J. J.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,4,23]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.2147\/tcrm.2006.2.3.229","article-title":"Oxycodone controlled release in cancer pain management","volume":"2","author":"G Biancofiore","year":"2006","journal-title":"Ther Clin Risk Manag"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1038\/431413a","article-title":"Analgesia: morphine-pathway block in top1 poppies","volume":"431","author":"AG Millgate","year":"2004","journal-title":"Nature"},{"key":"ref3","first-page":"92","article-title":"INCB Supply of opiate raw materials and demand for opiates for medical and scientific purposes","volume":"3","year":"2013","journal-title":"Estim World Requir 2014\u2014Stat 2012"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"3283","DOI":"10.1038\/ncomms4283","article-title":"Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae","volume":"5","author":"E Fossati","year":"2014","journal-title":"Nat Commun"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1038\/nchembio.1613","article-title":"A microbial biomanufacturing platform for natural and semisynthetic opioids","volume":"10","author":"K Thodey","year":"2014","journal-title":"Nat Chem Biol"},{"key":"ref6","first-page":"49","article-title":"Bench-top fermentative production of plant benzylisoquinoline alkaloids using a bacterial platform","volume":"3","author":"A Nakagawa","year":"2012","journal-title":"Bioeng Bugs"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1126\/science.1217410","article-title":"Mining the biodiversity of plants: a revolution in the making","volume":"336","author":"V De Luca","year":"2012","journal-title":"Science"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1093\/pcp\/pct020","article-title":"Benzylisoquinoline alkaloid metabolism: a century of discovery and a brave new world","volume":"54","author":"JM Hagel","year":"2013","journal-title":"Plant Cell Physiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00425-014-2056-8","article-title":"Benzylisoquinoline alkaloid biosynthesis in opium poppy","volume":"240","author":"GAW Beaudoin","year":"2014","journal-title":"Planta"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1074\/jbc.M803738200","article-title":"Structural basis of enzymatic (S)-norcoclaurine biosynthesis","volume":"284","author":"A Ilari","year":"2009","journal-title":"J Biol Chem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/j.phytochem.2004.02.015","article-title":"1,2-Dehydroreticuline synthase, the branch point enzyme opening the morphinan biosynthetic pathway","volume":"65","author":"K Hirata","year":"2004","journal-title":"Phytochemistry"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/0031-9422(92)80166-C","article-title":"Purification and properties of 1,2-dehydroreticuline reductase from Papaver somniferum seedlings","volume":"31","author":"W De-Eknamkul","year":"1992","journal-title":"Phytochemistry"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/nchembio.105","article-title":"Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiae","volume":"4","author":"KM Hawkins","year":"2008","journal-title":"Nat Chem Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"6695","DOI":"10.1038\/srep06695","article-title":"(R,S)-Tetrahydropapaveroline production by stepwise fermentation using engineered Escherichia coli","volume":"4","author":"A Nakagawa","year":"2014","journal-title":"Sci Rep"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.gene.2004.10.016","article-title":"Drag&amp;Drop cloning in yeast","volume":"344","author":"G Jansen","year":"2005","journal-title":"Gene"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e16","DOI":"10.1093\/nar\/gkn991","article-title":"DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways","volume":"37","author":"Z Shao","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nprot.2007.17","article-title":"Frozen competent yeast cells that can be transformed with high efficiency using the LiAc\/SS carrier DNA\/PEG method","volume":"2","author":"RD Gietz","year":"2007","journal-title":"Nat Protoc"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/ncomms1327","article-title":"A bacterial platform for fermentative production of plant alkaloids","volume":"2","author":"A Nakagawa","year":"2011","journal-title":"Nat Commun"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"24432","DOI":"10.1074\/jbc.M109.033373","article-title":"CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy","volume":"284","author":"A Gesell","year":"2009","journal-title":"J Biol Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1104\/pp.112.4.1669","article-title":"Molecular characterization of berberine bridge enzyme genes from opium poppy","volume":"112","author":"PJ Facchini","year":"1996","journal-title":"Plant Physiol"},{"key":"ref21","first-page":"249","article-title":"Thebaine synthase: a new enzyme in the morphine pathway in P. somniferum","volume":"2","author":"U Fisinger","year":"2007","journal-title":"Nat Prod Commun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1016\/S0031-9422(00)82630-8","article-title":"Neodihydrothebaine and bractazonine, two dibenz[d,f]azonine alkaloids of Papaver bracteatum","volume":"23","author":"HG Theuns","year":"1984","journal-title":"Phytochemistry"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0031-9422(00)90793-3","article-title":"Purification and characterization of salutaridine: NADPH 7-oxidoreductasefrom papaver somniferum","volume":"34","author":"R Gerardy","year":"1993","journal-title":"Phytochemistry"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/nchembio.317","article-title":"Dioxygenases catalyze the O-demethylation steps of morphine biosynthesis in opium poppy","volume":"6","author":"JM Hagel","year":"2010","journal-title":"Nat Chem Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.tibtech.2011.10.001","article-title":"Synthetic biosystems for the production of high-value plant metabolites","volume":"30","author":"PJ Facchini","year":"2012","journal-title":"Trends Biotechnol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1073\/pnas.92.6.2071","article-title":"Molecular cloning and heterologous expression of a cDNA encoding berbamunine synthase, a C-O phenol-coupling cytochrome P450 from the higher plant Berberis stolonifera","volume":"92","author":"PF Kraus","year":"1995","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref27","unstructured":"Sabarna K. Approaches to isolating a cDNA encoding thebaine synthase of morphine biosynthesis from opium poppy Papaver somniferum. PhD thesis, Martin Luther University Halle-Wittenberg. 2006. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.dart-europe.eu\/full.php?id=44110\" xlink:type=\"simple\">http:\/\/www.dart-europe.eu\/full.php?id=44110<\/ext-link>. Accessed: 26 August 2014."},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1098\/rsif.2011.0148","article-title":"Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae","volume":"8","author":"T Aabo","year":"2011","journal-title":"J R Soc Interface"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"26758","DOI":"10.1074\/jbc.M109.030957","article-title":"Removal of substrate inhibition and increase in maximal velocity in the short chain dehydrogenase\/reductase salutaridine reductase involved in morphine biosynthesis","volume":"284","author":"J Ziegler","year":"2009","journal-title":"J Biol Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1126\/science.1217411","article-title":"The rise of chemodiversity in plants","volume":"336","author":"J-K Weng","year":"2012","journal-title":"Science"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"28997","DOI":"10.1074\/jbc.M113.488585","article-title":"Dioxygenases catalyze O-demethylation and O,O-demethylenation with widespread roles in benzylisoquinoline alkaloid metabolism in opium poppy","volume":"288","author":"SC Farrow","year":"2013","journal-title":"J Biol Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1046\/j.1365-313X.1999.00470.x","article-title":"Molecular cloning and functional expression of codeinone reductase: the penultimate enzyme in morphine biosynthesis in the opium poppy Papaver somniferum","volume":"18","author":"B Unterlinner","year":"1999","journal-title":"Plant J"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1146\/annurev.arplant.59.032607.092730","article-title":"Alkaloid biosynthesis: metabolism and trafficking","volume":"59","author":"J Ziegler","year":"2008","journal-title":"Annu Rev Plant Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/biot.201300391","article-title":"A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments","volume":"9","author":"EY Kim","year":"2014","journal-title":"Biotechnol J"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/nbt.1557","article-title":"Synthetic protein scaffolds provide modular control over metabolic flux","volume":"27","author":"JE Dueber","year":"2009","journal-title":"Nat Biotechnol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.jbiotec.2013.04.004","article-title":"Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest","volume":"166","author":"M Xiao","year":"2013","journal-title":"J Biotechnol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0124459","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T05:04:46Z","timestamp":1540271086000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0124459"}},"subtitle":[],"editor":[{"given":"Alvaro","family":"Galli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,4,23]]},"references-count":36,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,4,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0124459","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,23]]}}}