{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T22:26:26Z","timestamp":1783031186135,"version":"3.54.6"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T00:00:00Z","timestamp":1429833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0124507","type":"journal-article","created":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T18:12:31Z","timestamp":1429899151000},"page":"e0124507","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":44,"title":["The Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Salmonella enterica serovar Typhi"],"prefix":"10.1371","volume":"10","author":[{"given":"Fenxia","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Kan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiying","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,4,24]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1086\/649541","article-title":"Global trends in typhoid and paratyphoid Fever","volume":"50","author":"JA Crump","year":"2010","journal-title":"Clin Infect Dis"},{"issue":"3","key":"ref2","first-page":"183","article-title":"Typhoid fever: a review of its impact and diagnostic problems","volume":"71","author":"PL Petit","year":"1994","journal-title":"East African medical journal"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","first-page":"670","DOI":"10.4269\/ajtmh.2004.70.670","article-title":"The etiology of febrile illness in adults presenting to Patan hospital in Kathmandu, Nepal","volume":"70","author":"DR Murdoch","year":"2004","journal-title":"Am J Trop Med Hyg"},{"key":"ref4","unstructured":"WHO. Background document: The diagnosis, treatment and prevention of typhoid fever. Communicable Disease Surveillance and Response Vaccines and Biologicals. 2003."},{"issue":"7558","key":"ref5","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1136\/bmj.333.7558.78","article-title":"Current concepts in the diagnosis and treatment of typhoid fever","volume":"333","author":"ZA Bhutta","year":"2006","journal-title":"BMJ"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.diagmicrobio.2008.03.018","article-title":"Clinical value of Tubex and Typhidot rapid diagnostic tests for typhoid fever in an urban community clinic in Bangladesh","volume":"61","author":"A Naheed","year":"2008","journal-title":"Diagnostic microbiology and infectious disease"},{"issue":"4","key":"ref7","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.diagmicrobio.2011.03.020","article-title":"Validation of the Dri-Dot Latex agglutination and IgM lateral flow assays for the diagnosis of typhoid fever in an Egyptian population","volume":"70","author":"I Nakhla","year":"2011","journal-title":"Diagnostic microbiology and infectious disease"},{"key":"ref8","unstructured":"Singh A, Verma HN, Arora K. Surface Plasmon Resonance Based Label-Free Detection of Salmonella using DNA Self Assembly. Applied biochemistry and biotechnology. 2014. Epub 2014\/11\/14."},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1128\/JCM.42.5.1885-1889.2004","article-title":"Evaluation of rapid diagnostic tests for typhoid fever","volume":"42","author":"SJ Olsen","year":"2004","journal-title":"Journal of clinical microbiology"},{"key":"ref10","unstructured":"Jaffery G, Hussain W, Saeed Anwer M, Maqbool S. Serological diagnosis of typhoid fever in childeren:a comparative evaluation of Salmonella typhi O-9 antigen based rapid assay. Presented at the Annual Pathology Conference, 2003, Pakistan and at the 17th International Biennial Conference of Pakistan Paediatric Association & 3rd Scientific Conference of Paediatric Association of SAARC Countries, 2004."},{"key":"ref11","unstructured":"Mahbubur Rahman P-LL, Sabrina Sharmin. Early diagnosis of typhoid fever in febrile children by rapid TUBEX-TF TF test. Presented at the 6th International Conference on Typhoid Fever and other Salmonelloses (WHO), 12th\u2014 14th. Guilin, China, November 2005,."},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1128\/JCM.01403-06","article-title":"Comparison of serological test kits for diagnosis of typhoid fever in the Philippines","volume":"45","author":"RL Kawano","year":"2007","journal-title":"Journal of clinical microbiology"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1017\/S0950268806007801","article-title":"Optimizing typhoid fever case definitions by combining serological tests in a large population study in Hechi City, China","volume":"135","author":"B Dong","year":"2007","journal-title":"Epidemiology and infection"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1017\/S0950268808000654","article-title":"Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samples","volume":"137","author":"A Ali","year":"2009","journal-title":"Epidemiology and infection"},{"issue":"8","key":"ref15","first-page":"1134","article-title":"Development of an ultra rapid and simple multiplex polymerase chain reaction technique for detection of Salmonella typhi","volume":"27","author":"K Ali","year":"2006","journal-title":"Saudi medical journal"},{"issue":"5","key":"ref16","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1128\/JCM.00109-08","article-title":"PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever","volume":"46","author":"H Levy","year":"2008","journal-title":"Journal of clinical microbiology"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s10156-003-0256-4","article-title":"Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi","volume":"9","author":"MN Massi","year":"2003","journal-title":"Journal of infection and chemotherapy: official journal of the Japan Society of Chemotherapy"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1007\/s12098-007-0167-y","article-title":"Diagnosis of typhoid fever by polymerase chain reaction","volume":"74","author":"SR Ambati","year":"2007","journal-title":"Indian journal of pediatrics"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1574-695X.2011.00785.x","article-title":"Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications","volume":"62","author":"P Francois","year":"2011","journal-title":"FEMS Immunol Med Microbiol"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jbbm.2003.12.005","article-title":"Real-time turbidimetry of LAMP reaction for quantifying template DNA","volume":"59","author":"Y Mori","year":"2004","journal-title":"J Biochem Biophys Methods"},{"issue":"12","key":"ref21","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"T Notomi","year":"2000","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"ref22","doi-asserted-by":"crossref","first-page":"7046","DOI":"10.1128\/AEM.70.12.7046-7052.2004","article-title":"Diagnostic real-time PCR for detection of Salmonella in food","volume":"70","author":"B Malorny","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"ref23","unstructured":"He PY. The investigation of the influence of S. Typhi plasmid pRST98 and spv genes on macrophage autophagy and apoptosis and the molecular mechanisms. PhD thesis. SuZhou University. 2012."},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1128\/JCM.36.6.1683-1687.1998","article-title":"Quantitation of bacteria in blood of typhoid fever patients and relationship between counts and clinical features, transmissibility, and antibiotic resistance","volume":"36","author":"J Wain","year":"1998","journal-title":"Journal of clinical microbiology"},{"issue":"6","key":"ref25","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1586\/eri.11.47","article-title":"The utility of diagnostic tests for enteric fever in endemic locations","volume":"9","author":"CM Parry","year":"2011","journal-title":"Expert Rev Anti Infect Ther"},{"issue":"4","key":"ref26","first-page":"325","article-title":"Development of RT-LAMP technique to detect Salmonella Typhi pathogen in blood","volume":"27","author":"FX Fan","year":"2012","journal-title":"Disease Surveilance"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1574-695X.2011.00785.x","article-title":"Robustness ofa loop-mediated isothermalampli\u00a2cation reaction for diagnostic applications","volume":"62","author":"MT Patrice Francois","year":"2011","journal-title":"FEMS Immunol Med Microbiol"},{"issue":"5","key":"ref28","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/00365510600791724","article-title":"Enhanced detection rate of typhoid fever in children in a periurban slum in Karachi, Pakistan using polymerase chain reaction technology","volume":"66","author":"SQ Nizami","year":"2006","journal-title":"Scandinavian journal of clinical and laboratory investigation"},{"issue":"7","key":"ref29","first-page":"157","article-title":"Typhoid hepatitis: report of two cases","volume":"49","author":"V Sharma","year":"1995","journal-title":"Indian journal of medical sciences"},{"issue":"5","key":"ref30","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1007\/s10529-010-0211-2","article-title":"Suman. Quick PCR based diagnosis of typhoid using specific genetic markers","volume":"32","author":"A Kumar","year":"2010","journal-title":"Biotechnology letters"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1365-3156.2005.01538.x","article-title":"Sero-epidemiological study of kala-azar in a village of Varanasi district, India","volume":"11","author":"R Kumar","year":"2006","journal-title":"Tropical medicine & international health: TM & IH"},{"issue":"4","key":"ref32","first-page":"913","article-title":"Status of Vi gene, its expression and Salmonella Pathogenicity Island (SPI-7) in Salmonella Typhi in India","volume":"41","author":"P Maurya","year":"2010","journal-title":"The Southeast Asian journal of tropical medicine and public health"},{"issue":"9","key":"ref33","doi-asserted-by":"crossref","first-page":"4418","DOI":"10.1128\/JCM.43.9.4418-4425.2005","article-title":"Detection of Vi-negative Salmonella enterica serovar typhi in the peripheral blood of patients with typhoid fever in the Faisalabad region of Pakistan","volume":"43","author":"S Baker","year":"2005","journal-title":"Journal of clinical microbiology"},{"issue":"12","key":"ref34","doi-asserted-by":"crossref","first-page":"e52486","DOI":"10.1371\/journal.pone.0052486","article-title":"Evaluation of a direct reverse transcription loop-mediated isothermal amplification method without RNA extraction for the detection of human enterovirus 71 subgenotype C4 in nasopharyngeal swab specimens","volume":"7","author":"K Nie","year":"2012","journal-title":"PloS one"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0124507","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T08:57:56Z","timestamp":1598950676000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0124507"}},"subtitle":[],"editor":[{"given":"Praveen","family":"Rishi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,4,24]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,4,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0124507","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,24]]}}}