{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:12:22Z","timestamp":1770883942983,"version":"3.50.1"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T00:00:00Z","timestamp":1429228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0125195","type":"journal-article","created":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T18:57:00Z","timestamp":1429297020000},"page":"e0125195","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Up-Regulation of TREK-2 Potassium Channels in Cultured Astrocytes Requires De Novo Protein Synthesis: Relevance to Localization of TREK-2 Channels in Astrocytes after Transient Cerebral Ischemia"],"prefix":"10.1371","volume":"10","author":[{"given":"Aixa F.","family":"Rivera-Pag\u00e1n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"Rivera-Aponte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katya V.","family":"Melnik-Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astrid","family":"Zayas-Santiago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilia Y.","family":"Kucheryavykh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio H.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A.","family":"Cubano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serguei N.","family":"Skatchkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misty J.","family":"Eaton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,4,17]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1460-9568.2011.07923.x","article-title":"How many neurons do you have? Some dogmas of quantitative neuroscience","volume":"35","author":"R Lent","year":"2012","journal-title":"Eur. J. Neurosci."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1152\/jn.00996.2005","article-title":"Lack of the Kir4.1 channel subunit abolishes K+ buffering properties of astrocytes in the ventral respiratory group: impact on extracellular K+ regulation","volume":"95","author":"C Neusch","year":"2006","journal-title":"J. Neurophysiol."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1002\/glia.20455","article-title":"Downregulation of Kir4.1 inward rectifying potassium channel subunits by RNAi impairs potassium transfer and glutamate uptake by cortical astrocytes","volume":"55","author":"YV Kucheryavykh","year":"2007","journal-title":"Glia"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1152\/jn.00340.2007","article-title":"Differential distribution of Kir4.1 in spinal cord astrocytes suggest regional differences in K+ homeostasis","volume":"98","author":"ML Olsen","year":"2007","journal-title":"J. Neurophysiol."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"7474","DOI":"10.1523\/JNEUROSCI.3790-08.2009","article-title":"Analysis of astroglial K+ channel expression in the developing hippocampus reveals a predominant role of the Kir4.1 subunit","volume":"29","author":"G Seifert","year":"2009","journal-title":"J. Neurosci."},{"key":"ref6","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/S0006-8993(02)02261-8","article-title":"Functional expression of TREK-2 K+ channel in cultured rat brain astrocytes","volume":"931","author":"C Gnatenco","year":"2002","journal-title":"Brain Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"40","DOI":"10.2174\/1874082000903010040","article-title":"Ischemia increases TREK-2 channel expression in astrocytes: Relevance to glutamate clearance","volume":"3","author":"LY Kucheryavykh","year":"2009","journal-title":"Open J. Neurosci."},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.mehy.2007.06.016","article-title":"Recent advance and possible future in TREK-2: a two-pore potassium channel may involved in the process of NPP, brain ischemia and memory impairment","volume":"70","author":"D Huang","year":"2008","journal-title":"Med Hypotheses"},{"issue":"7","key":"ref9","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.neuint.2010.08.016","article-title":"Impact of global cerebral ischemia on K+ channel expression and membrane properties of glial cells in the rat hippocampus","volume":"57","author":"H Pivonkova","year":"2010","journal-title":"Neurochem Int"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s12031-012-9875-5","article-title":"Involvement of TREK-1 activity in astrocyte function and neuroprotection under simulated ischemia conditions","volume":"49","author":"X Wu","year":"2013","journal-title":"J Mol Neurosci"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"e97155","DOI":"10.1371\/journal.pone.0097155","article-title":"Unidirectional photoreceptor-to-M\u00fcller glia coupling and unique K+ channel expression in caiman retina","volume":"9","author":"A Zayas-Santiago","year":"2014","journal-title":"PLoS ONE"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1002\/jnr.10580","article-title":"Arachidonic acid activates an open rectifier potassium channel in cultured rat cortical astrocytes","volume":"72","author":"S Ferroni","year":"2003","journal-title":"J Neurosci Res"},{"issue":"12","key":"ref13","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.tips.2003.10.008","article-title":"Fatty acid-sensitive two-pore domain K+ channels","volume":"24","author":"D Kim","year":"2003","journal-title":"Trends Pharmacol Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1471-4159.1982.tb10857.x","article-title":"Brain cortical fatty acids and phospholipids during and following complete and severe incomplete ischemia","volume":"38","author":"S Rehncrona","year":"1982","journal-title":"J. Neurochem."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/j.1749-6632.1991.tb15622.x","article-title":"Glial ion transport and volume control","volume":"633","author":"O Kempski","year":"1991","journal-title":"Annals of the NY Academy of Science"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1089\/neu.1999.16.831","article-title":"Effect of lactacidosis on cell volume and intracellular pH of astrocytes","volume":"16","author":"N Plesnila","year":"1999","journal-title":"J. Neurotrauma."},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1016\/j.bbrc.2004.12.124","article-title":"Enhanced expressions of arachidonic acid-sensitive tandem-pore domain potassium channels in rat experimental acute cerebral ischemia","volume":"327","author":"ZB Li","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.molbrainres.2003.09.020","article-title":"Alterations in the expression of lipid and mechano-gated two pore domain potassium channel genes in rat brain following chronic cerebral ischemia","volume":"120","author":"X Xu","year":"2004","journal-title":"Brain Res Mol Brain.Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/S0968-0004(03)00051-3","article-title":"Regulation of mRNA translation by 5\u2019- and 3\u2019 UTR-binding factors","volume":"28","author":"GS Wilkie","year":"2003","journal-title":"Trends Biochem Sci"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1126\/science.310.5749.764","article-title":"P-bodies mark the spot for controlling protein production","volume":"310","author":"J Marx","year":"2005","journal-title":"Science"},{"issue":"2","key":"ref21","doi-asserted-by":"crossref","first-page":"e56948","DOI":"10.1371\/journal.pone.0056948","article-title":"Regulation of the membrane insertion and conductance activity of the metamorphic chloride intracellular channel protein CLIC1 by cholesterol","volume":"8","author":"SM Valenzuela","year":"2013","journal-title":"PLoS ONE"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6617","DOI":"10.1016\/S0021-9258(18)50413-0","article-title":"Inhibition of protein degradation by anoxia and ischemia in perfused rat hearts","volume":"254","author":"B Chua","year":"1979","journal-title":"J Biol. Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.brainres.2005.11.090","article-title":"Expression Analysis Systematic Explorer (EASE) analysis reveals differential gene expression in permanent and transient focal stroke rat models","volume":"1071","author":"G Ford","year":"2006","journal-title":"Brain Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1006\/mcne.2001.1048","article-title":"Kir 6.1 is the principal pore-forming subunit of astrocyte but not neuronal plasma membrane KATP channels","volume":"18","author":"A Thomzig","year":"2001","journal-title":"Mol Cell Neurosci"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/glia.20280","article-title":"Tandem-pore domain potassium channels are functionally expressed in retinal (M\u00fcller) glial cells","volume":"53","author":"SN Skatchkov","year":"2006","journal-title":"Glia"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1006\/exnr.1994.1047","article-title":"Hypoxic-ischemic brain injury stimulates glial fibrillary acidic protein mRNA and protein expression in neonatal rats","volume":"126","author":"D Burtrum","year":"1994","journal-title":"Exp Neurol"},{"issue":"2\u20133","key":"ref27","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0014-5793(02)02364-5","article-title":"Radiation-induced apoptosis in MOLT-4 cells requires de novo protein synthesis independent of de novo RNA synthesis","volume":"514","author":"MH Taylor","year":"2002","journal-title":"FEBS Lett"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1104\/pp.61.3.314","article-title":"De novo messenger RNA and protein synthesis are required for phytoalexin-mediated disease resistance in soybean hypocotyls","volume":"61","author":"M Yoshikawa","year":"1978","journal-title":"Plant Physiol"},{"issue":"19","key":"ref29","first-page":"III312","article-title":"Ischemic but not pharmacological preconditioning requires protein synthesis","volume":"102","author":"N Matsuyama","year":"2000","journal-title":"Circulation"},{"issue":"7","key":"ref30","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1681\/ASN.2006010083","article-title":"Protein degradation by the ubiquitin-proteasome pathway in normal and disease states","volume":"17","author":"SH Lecker","year":"2006","journal-title":"J Am Soc Nephrol"},{"key":"ref31","first-page":"112","article-title":"Regulation through degradation","volume":"3","author":"J Whitfield","year":"2003","journal-title":"Nature Rev. Mol. Cell Biol."},{"issue":"9","key":"ref32","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/nmeth.1686","article-title":"Regulation through degradation","volume":"8","author":"A Doerr","year":"2011","journal-title":"Nat Methods"},{"issue":"23","key":"ref33","doi-asserted-by":"crossref","first-page":"17412","DOI":"10.1074\/jbc.M000445200","article-title":"TREK-2, a new member of the mechanosensitive tandem-pore K+ channel family","volume":"275","author":"H Bang","year":"2000","journal-title":"J Biol Chem"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1038\/jcbfm.1993.74","article-title":"Characterization of cortical depolarizations evoked in focal cerebral ischemia","volume":"13","author":"M Nedergaard","year":"1993","journal-title":"J Cereb Blood Flow Metab"},{"issue":"2","key":"ref35","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/(SICI)1098-1136(199702)19:2<123::AID-GLIA4>3.0.CO;2-1","article-title":"Stimulation of glutamate uptake and Na,K-ATPase activity in rat astrocytes exposed to ischemia-like insults","volume":"19","author":"DB Stanimirovic","year":"1997","journal-title":"Glia"},{"issue":"11","key":"ref36","doi-asserted-by":"crossref","first-page":"3970","DOI":"10.1128\/MCB.00128-07","article-title":"P-body formation is a consequence, not the cause, of RNA-mediated gene silencing","volume":"27","author":"A Eulalio","year":"2007","journal-title":"Mol Cell Biol"},{"issue":"5","key":"ref37","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.molcel.2007.02.011","article-title":"P bodies and the control of mRNA translation and degradation","volume":"25","author":"R Parker","year":"2007","journal-title":"Mol Cell"},{"issue":"7","key":"ref38","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1038\/ncb1274","article-title":"MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies","volume":"7","author":"J Liu","year":"2005","journal-title":"Nat Cell Biol"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1038\/sj.jcbfm.9600546","article-title":"Protective role of reactive astrocytes in brain ischemia","volume":"28","author":"L Li","year":"2008","journal-title":"J Cereb Blood Flow Metab"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0125195","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T10:48:55Z","timestamp":1651747735000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0125195"}},"subtitle":[],"editor":[{"given":"Thiruma V.","family":"Arumugam","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,4,17]]},"references-count":39,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,4,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0125195","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,17]]}}}