{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T06:44:23Z","timestamp":1773470663548,"version":"3.50.1"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0125502","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T18:11:10Z","timestamp":1431540670000},"page":"e0125502","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Parameters Influencing Baseline HIV-1 Genotypic Tropism Testing Related to Clinical Outcome in Patients on Maraviroc"],"prefix":"10.1371","volume":"10","author":[{"given":"Saleta","family":"Sierra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Nikolai","family":"Dybowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Pironti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominik","family":"Heider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"G\u00fcney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Thielen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Reuter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Esser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerd","family":"F\u00e4tkenheuer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Lengauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert","family":"Pfister","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u00f6rn","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"Kaiser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,5,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1086\/650697","article-title":"Maraviroc versus efavirenz, both in combination with zidovudine-lamivudine, for the treatment of antiretroviral-naive subjects with CCR5-tropic HIV-1 infection","volume":"201","author":"DA Cooper","year":"2010","journal-title":"J Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1056\/NEJMoa0803152","article-title":"Maraviroc for previously treated patients with R5 HIV-1 infection","volume":"359","author":"RM Gulick","year":"2008","journal-title":"N Engl J Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1056\/NEJMoa0803154","article-title":"Subgroup analyses of maraviroc in previously treated R5 HIV-1 infection","volume":"359","author":"G Fatkenheuer","year":"2008","journal-title":"N Engl J Med"},{"key":"ref4","first-page":"463","article-title":"Phenotypic assays for the determination of coreceptor tropism in HIV-1 infected individuals","volume":"12","author":"P Braun","year":"2007","journal-title":"Eur J Med Res"},{"key":"ref5","first-page":"195","article-title":"Performance of genotypic tropism testing in clinical practice using the enhanced sensitivity version of Trofile as reference assay: results from the OSCAR Study Group","volume":"33","author":"V Svicher","year":"2010","journal-title":"New Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"3335","DOI":"10.1128\/AAC.00148-10","article-title":"Evaluation of the genotypic prediction of HIV-1 coreceptor use versus a phenotypic assay and correlation with the virological response to maraviroc: the ANRS GenoTropism study","volume":"54","author":"P Recordon-Pinson","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1186\/2047-783X-15-6-231","article-title":"Maraviroc in treatment-experienced patients with HIV-1 infection\u2014experience from routine clinical practice","volume":"15","author":"S Reuter","year":"2010","journal-title":"Eur J Med Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1097\/COH.0b013e328356e9da","article-title":"Next-generation sequencing to assess HIV tropism","volume":"7","author":"LC Swenson","year":"2012","journal-title":"Curr Opin HIV AIDS"},{"key":"ref9","first-page":"16","article-title":"High Resolution Tropism Kinetics by Quantitative Deep Sequencing in HIV-1-infected Subjects Initiating Suppressive First-line ART","author":"C Pou","year":"2010"},{"issue":"Suppl. 4","key":"ref10","doi-asserted-by":"crossref","first-page":"P128","DOI":"10.1186\/1758-2652-13-S4-P128","article-title":"Population and ultra-deep sequencing for tropism determination are correlated with Trofile ES: genotypic re-analysis of the A4001078 maraviroc study","volume":"13","author":"S Portsmouth","year":"2010","journal-title":"Journal of the International AIDS Society"},{"key":"ref11","article-title":"Relative Performance of ESTA, Trofile, 454 Deep Sequencing, and \u201cReflex\u201d Testing for HIV Tropism in the MOTIVATE Screening Population of Therapy-experienced Patients","author":"CJ Brumme","year":"2011"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1093\/infdis\/jiq030","article-title":"Deep sequencing to infer HIV-1 co-receptor usage: application to three clinical trials of maraviroc in treatment-experienced patients","volume":"203","author":"LC Swenson","year":"2011","journal-title":"J Infect Dis"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1093\/cid\/cir493","article-title":"Deep V3 sequencing for HIV type 1 tropism in treatment-naive patients: a reanalysis of the MERIT trial of maraviroc","volume":"53","author":"LC Swenson","year":"2011","journal-title":"Clin Infect Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/S1473-3099(10)70319-4","article-title":"European guidelines on the clinical management of HIV-1 tropism testing","volume":"11","author":"LP Vandekerckhove","year":"2011","journal-title":"Lancet Infect Dis"},{"key":"ref15","unstructured":"Eberle J, Noah C, Wolf E, St\u00fcrmer M, Braun P, Korn K, et al. (2014) Empfehlungen zur Bestimmung des HIV-1-Korezeptor-Gebrauchs (DAIG recommendations for the HIV-1 tropism testing)."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e58","DOI":"10.1371\/journal.pcbi.0030058","article-title":"Structural descriptors of gp120 V3 loop for the prediction of HIV-1 coreceptor usage","volume":"3","author":"O Sander","year":"2007","journal-title":"PLoS Computational Biology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e1000743","DOI":"10.1371\/journal.pcbi.1000743","article-title":"Prediction of co-receptor usage of HIV-1 from genotype","volume":"6","author":"JN Dybowski","year":"2010","journal-title":"PLoS Comput Biol"},{"key":"ref18","first-page":"3624","article-title":"Prediction of HIV-1 coreceptor usage (tropism) by sequence analysis using a genotypic approach","volume":"58","author":"S Sierra","year":"2011","journal-title":"J Vis Exp"},{"key":"ref19","first-page":"A78","article-title":"Validation of HIV-1 drug resistance testing by deep sequencing: insights from comparative Sanger sequencing","volume":"19","author":"A Thielen","year":"2014","journal-title":"Antiviral Ther"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1126\/science.3277272","article-title":"The brain in AIDS: central nervous system HIV-1 infection and AIDS dementia complex","volume":"239","author":"RW Price","year":"1988","journal-title":"Science"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1097\/QAI.0b013e3181ba46a6","article-title":"Are the proposed env mutations actually associated with resistance to maraviroc?","volume":"53","author":"A Saracino","year":"2010","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref22","article-title":"Molecular basis of human immunodeficiency virus type 1 drug resistance: Overview and recent developments","author":"L Menendez-Arias","year":"2013","journal-title":"Antiviral Res"},{"key":"ref23","first-page":"16","article-title":"Population-based Sequencing of the V3-loop Is Comparable to the Enhanced Sensitivity Trofile Assay in Predicting Virologic Response to Maraviroc of Treatment-na\u00efve Patients in the MERIT Trial","author":"R McGovern","year":"2010"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1089\/aid.2011.0251","article-title":"Concordance of HIV Type 1 Tropism Phenotype to Predictions Using Web-Based Analysis of V3 Sequences: Composite Algorithms May Be Needed to Properly Assess Viral Tropism","volume":"28","author":"GB Cabral","year":"2012","journal-title":"AIDS Res Hum Retroviruses"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1097\/COH.0b013e328356eaa7","article-title":"HIV population genotypic tropism testing and its clinical significance","volume":"7","author":"M Obermeier","year":"2012","journal-title":"Curr Opin HIV AIDS"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/j.1468-1293.2011.00922.x","article-title":"Concordance between HIV-1 genotypic coreceptor tropism predictions based on plasma RNA and proviral DNA","volume":"12","author":"C Verhofstede","year":"2011","journal-title":"HIV Med"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1742-4690-7-56","article-title":"Comparative determination of HIV-1 co-receptor tropism by Enhanced Sensitivity Trofile, gp120 V3-loop RNA and DNA genotyping","volume":"7","author":"MC Prosperi","year":"2010","journal-title":"Retrovirology"},{"key":"ref28","first-page":"23","article-title":"Tropism testing from proviral DNA\u2014analysis of a subgroup from the Berlin Maraviroc cohort","volume":"1","author":"M Obermeier","year":"2010","journal-title":"Reviews in Antiviral"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1097\/COH.0b013e328356e9c2","article-title":"Coreceptor usage in different reservoirs","volume":"7","author":"C Soulie","year":"2012","journal-title":"Curr Opin HIV AIDS"},{"key":"ref30","article-title":"Use of Cellular HIV DNA to Predict Virologic Response to Maraviroc: Performance of Population-Based and Deep Sequencing","author":"LC Swenson","year":"2013","journal-title":"Clin Infect Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1159\/000332003","article-title":"The XTrack System: Application and Advantage","volume":"55","author":"T Klimkait","year":"2012","journal-title":"Intervirology"},{"key":"ref32","first-page":"17","article-title":"The influence of PCR amplification variation on the ability of population-based PCR to detect non-R5 HIV","author":"PR Harrigan","year":"2010"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1128\/JCM.00739-12","article-title":"Factors influencing the sensitivity and specificity of conventional sequencing in human immunodeficiency virus type 1 tropism testing","volume":"51","author":"DJ Knapp","year":"2013","journal-title":"J Clin Microbiol"},{"key":"ref34","first-page":"135","article-title":"Genotypic determination of HIV tropism\u2014clinical and methodological recommendations to guide the therapeutic use of CCR5 antagonists","volume":"12","author":"E Poveda","year":"2010","journal-title":"AIDS Rev"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.antiviral.2012.11.002","article-title":"Correlation between genotypic (V3 population sequencing) and phenotypic (Trofile ES) methods of characterizing co-receptor usage of HIV-1 from 200 treatment-naive HIV patients screened for Study A4001078","volume":"97","author":"S Portsmouth","year":"2013","journal-title":"Antiviral Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1097\/QAI.0b013e31826249cf","article-title":"Population-based sequencing of the V3-loop can predict the virological response to maraviroc in treatment-naive patients of the MERIT trial","volume":"61","author":"RA McGovern","year":"2012","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref37","article-title":"Calibration and accuracy of the geno2pheno co-receptor algorithm for predicting HIV tropism for single and triplicate measurements of V3 genotype","author":"LC Swenson","year":"2010","journal-title":"Journal of the International AIDS Society."},{"key":"ref38","article-title":"Impact of triplicate testing on genotypic HIV-1 tropism prediction in routine clinical practice","author":"J Symons","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"4909","DOI":"10.1128\/JVI.80.10.4909-4920.2006","article-title":"Emergence of CXCR4-using human immunodeficiency virus type 1 (HIV-1) variants in a minority of HIV-1-infected patients following treatment with the CCR5 antagonist maraviroc is from a pretreatment CXCR4-using virus reservoir","volume":"80","author":"M Westby","year":"2006","journal-title":"J Virol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1093\/jac\/dks055","article-title":"Evolution of proviral DNA HIV-1 tropism under selective pressure of maraviroc-based therapy","volume":"67","author":"S Baroncelli","year":"2012","journal-title":"J Antimicrob Chemother"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"597","DOI":"10.3109\/08820139.2011.569673","article-title":"HIV-1 co-receptor usage based on V3 loop sequence analysis: preferential suppression of CXCR4 virus post HAART?","volume":"40","author":"Y Jiao","year":"2011","journal-title":"Immunol Invest"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"e80259","DOI":"10.1371\/journal.pone.0080259","article-title":"Frequency and predictors of HIV-1 co-receptor switch in treatment naive patients","volume":"8","author":"V Mortier","year":"2013","journal-title":"PLoS One"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.virol.2011.10.005","article-title":"The evolution of human immunodeficiency virus type-1 (HIV-1) envelope molecular properties and coreceptor use at all stages of infection in an HIV-1 donor-recipient pair","volume":"422","author":"D Edo-Matas","year":"2012","journal-title":"Virology"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1182\/blood-2010-10-311860","article-title":"Interleukin-7 induces HIV type 1 R5-to-X4 switch","volume":"117","author":"N Brieu","year":"2011","journal-title":"Blood"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"5452","DOI":"10.1128\/JVI.74.12.5452-5459.2000","article-title":"Polymorphism in the interleukin-4 promoter affects acquisition of human immunodeficiency virus type 1 syncytium-inducing phenotype","volume":"74","author":"EE Nakayama","year":"2000","journal-title":"J Virol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1093\/infdis\/169.6.1244","article-title":"Associations between HLA frequencies and pathogenic features of human immunodeficiency virus type 1 infection in seroconverters from the Amsterdam cohort of homosexual men","volume":"169","author":"MR Klein","year":"1994","journal-title":"J Infect Dis"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1086\/515307","article-title":"CCR5\/delta(ccr5) heterozygosity: a selective pressure for the syncytium-inducing human immunodeficiency virus type 1 phenotype. NIAID AIDS Clinical Trials Group Protocol 241 Virology Team","volume":"177","author":"RT D'Aquila","year":"1998","journal-title":"J Infect Dis"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"395","DOI":"10.3851\/IMP1759","article-title":"Baseline CD4(+) T-cell counts and weighted background susceptibility scores strongly predict response to maraviroc regimens in treatment-experienced patients","volume":"16","author":"JM Schapiro","year":"2011","journal-title":"Antivir Ther"},{"key":"ref49","first-page":"7","article-title":"Short-term variation of HIV tropism readouts in the absence of CCR5 antagonists","author":"CJ Brumme","year":"2010","journal-title":"Journal of the International AIDS Society"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"S17","DOI":"10.1016\/j.jana.2006.11.020","article-title":"Strategies for improving patient adherence to therapy and long-term patient outcomes","volume":"18","author":"AM Battaglioli-DeNero","year":"2007","journal-title":"J Assoc Nurses AIDS Care"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1093\/jac\/dks362","article-title":"Pitfalls of HIV genotypic tropism testing after treatment interruption","volume":"68","author":"M Wirden","year":"2013","journal-title":"J Antimicrob Chemother"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0125502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T01:32:02Z","timestamp":1588987922000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0125502"}},"subtitle":[],"editor":[{"given":"Francesca","family":"Ceccherini-Silberstein","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,13]]},"references-count":51,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0125502","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,13]]}}}