{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:59:09Z","timestamp":1787021949744,"version":"build-2736575974"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T00:00:00Z","timestamp":1430352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0125667","type":"journal-article","created":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T16:17:36Z","timestamp":1430410656000},"page":"e0125667","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":60,"title":["Berberine Suppresses Adipocyte Differentiation via Decreasing CREB Transcriptional Activity"],"prefix":"10.1371","volume":"10","author":[{"given":"Juan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongju","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruyuan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,4,30]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1038\/35007508","article-title":"Obesity as a medical problem","volume":"404","author":"PG Kopelman","year":"2000","journal-title":"Nature"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"700","DOI":"10.5483\/BMBRep.2012.45.12.235","article-title":"Involvement of protein tyrosine phosphatases in adipogenesis:New anti-obesity targets?","volume":"45","author":"KW Bae","year":"2012","journal-title":"BMB Reports"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1258\/ebm.2010.010063","article-title":"Cell line models for differentiation: preadipocytes and adipocytes","volume":"235","author":"SP Poulos","year":"2010","journal-title":"Exp Biol Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e1001436","DOI":"10.1371\/journal.pbio.1001436","article-title":"The fat controller: adipocyte development","volume":"10","author":"JM Stephens","year":"2012","journal-title":"PLoS Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1128\/MCB.00709-07","article-title":"Cyclic AMP (cAMP)-mediated stimulation of adipocyte differentiation requires the synergistic action of Epac- and cAMP-dependent protein kinase-dependent processes","volume":"28","author":"RK Petersen","year":"2008","journal-title":"Mol Cell Biol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.1210\/jc.2007-2404","article-title":"Treatment of type 2 diabetes and dyslipidemia with the natural plant alkaloid berberine","volume":"93","author":"Y Zhang","year":"2008","journal-title":"J Clin Endocrinol Metab"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.2337\/db06-0006","article-title":"Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states","volume":"55","author":"YS Lee","year":"2006","journal-title":"Diabetes"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"4510","DOI":"10.1210\/en.2007-1752","article-title":"Berberine acutely inhibits insulin secretion from beta-cells through 3',5'-cyclic adenosine 5'-monophosphate signaling pathway","volume":"149","author":"L Zhou","year":"2008","journal-title":"Endocrinology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.bbrc.2011.08.110","article-title":"Berberine exerts anti-adipogenic activity through up-regulation of C\/EBP inhibitors, CHOP and DEC2","volume":"413","author":"TP Pham","year":"2011","journal-title":"Biochem Biophys Res Commun"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1002\/ptr.3204","article-title":"Berberine inhibits SREBP-1-related clozapine and risperidone induced adipogenesis in 3T3-L1 cells","volume":"24","author":"Y Hu","year":"2010","journal-title":"Phytother Res"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/ptr.3493","article-title":"Inhibition of preadipocyte differentiation and lipid accumulation by Orengedokuto treatment of 3T3-L1 cultures","volume":"26","author":"N Ikarashi","year":"2012","journal-title":"Phytother Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"5493","DOI":"10.1038\/ncomms6493","article-title":"Berberine activates thermogenesis in white and brown adipose tissue","volume":"5","author":"Z Zhang","year":"2014","journal-title":"Nat Commun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.2337\/db07-1552","article-title":"Berberine and its more biologically available derivative, dihydroberberine, inhibit mitochondrial respiratory complex I: a mechanism for the action of berberine to activate AMP-activated protein kinase and improve insulin action","volume":"57","author":"N Turner","year":"2008","journal-title":"Diabetes"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1194\/jlr.M600020-JLR200","article-title":"Inhibition of lipid synthesis through activation of AMP kinase: an additional mechanism for the hypolipidemic effects of berberine","volume":"47","author":"JM Brusq","year":"2006","journal-title":"J Lipid Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.bbadis.2010.10.001","article-title":"Berberine attenuates cAMP-induced lipolysis via reducing the inhibition of phosphodiesterase in 3T3-L1 adipocytes","volume":"1812","author":"L Zhou","year":"2011","journal-title":"Biochim Biophys Acta"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.2119\/molmed.2011.00163","article-title":"Small-molecule activators of AMP-activated protein kinase (AMPK), RSVA314 and RSVA405, inhibit adipogenesis","volume":"17","author":"V Vingtdeux","year":"2011","journal-title":"Mol Med"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"13346","DOI":"10.1021\/jf203490a","article-title":"Antiadipogenic effect of dietary apigenin through activation of AMPK in 3T3-L1 cells","volume":"59","author":"M Ono","year":"2011","journal-title":"J Agric Food Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1016\/j.jnutbio.2011.10.008","article-title":"Thiacremonone, a sulfur compound isolated from garlic, attenuates lipid accumulation partially mediated via AMPK activation in 3T3-L1 adipocytes","volume":"23","author":"EJ Kim","year":"2012","journal-title":"J Nutr Biochem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1016\/j.bbrc.2007.10.125","article-title":"Anti-obesity effects of ginsenoside Rh2 are associated with the activation of AMPK signaling pathway in 3T3-L1 adipocyte","volume":"364","author":"JT Hwang","year":"2007","journal-title":"Biochem Biophys Res Commun"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.metabol.2006.10.025","article-title":"Berberine stimulates glucose transport through a mechanism distinct from insulin","volume":"56","author":"L Zhou","year":"2007","journal-title":"Metabolism"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.abb.2008.07.029","article-title":"Compound C inhibits clonal expansion of preadipocytes by increasing p21 level irrespectively of AMPK inhibition","volume":"479","author":"M Nam","year":"2008","journal-title":"Arch Biochem Biophys"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1074\/jbc.M311327200","article-title":"Role of CREB in transcriptional regulation of CCAAT\/enhancer-binding protein beta gene during adipogenesis","volume":"279","author":"JW Zhang","year":"2004","journal-title":"J Biol Chem"},{"key":"ref23","first-page":"2037","article-title":"Activation of extracellular signal-regulated kinases and CREB\/ATF-1 mediate the expression of CCAAT\/enhancer binding proteins beta and-delta in preadipocytes","volume":"5","author":"N Belmonte","year":"2001","journal-title":"Mol Endocrinol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e1540","DOI":"10.1371\/journal.pone.0001540","article-title":"cAMP\/PKA regulates osteogenesis, adipogenesis and ratio of RANKL\/OPG mRNA expression in mesenchymal stem cells by suppressing leptin","volume":"3","author":"DC Yang","year":"2008","journal-title":"PLoS One"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"40341","DOI":"10.1074\/jbc.M605077200","article-title":"Depletion of cAMP-response element-binding protein\/ATF1 inhibits adipogenic conversion of 3T3-L1 cells ectopically expressing CCAAT\/enhancer-binding protein (C\/EBP) alpha, C\/EBP beta, or PPAR gamma 2","volume":"281","author":"KE Fox","year":"2006","journal-title":"J Biol Chem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"8886","DOI":"10.1074\/jbc.M310190200","article-title":"Dioxin increases C\/EBPbeta transcription by activating cAMP\/protein kinase A","volume":"279","author":"CF Vogel","year":"2004","journal-title":"J Biol Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/MCB.20.3.1008-1020.2000","article-title":"CREB activation induces adipogenesis in 3T3-L1 cells","volume":"2","author":"JE Reusch","year":"2000","journal-title":"Mol Cell Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.cmet.2009.03.012","article-title":"AMPK: an emerging drug target for diabetes and the metabolic syndrome","volume":"9","author":"BB Zhang","year":"2000","journal-title":"Cell Metab"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1432-1033.1997.00259.x","article-title":"The AMP-activated protein kinase-fuel gauge of the mammalian cell?","volume":"246","author":"DG Hardie","year":"1997","journal-title":"Eur J Biochem"},{"key":"ref30","first-page":"E1","article-title":"AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes","volume":"277","author":"WW Winder","year":"1999","journal-title":"Am J Physiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1194\/jlr.M022897","article-title":"AMPK activation with glabridin ameliorates adiposity and lipid dysregulation in obesity","volume":"53","author":"JW Lee","year":"2012","journal-title":"J Lipid Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e70135","DOI":"10.1371\/journal.pone.0070135","article-title":"Ursolic acid inhibits adipogenesis in 3T3-L1 adipocytes through LKB1\/AMPK pathway","volume":"8","author":"Y He","year":"2013","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.1038\/oby.2007.309","article-title":"Inhibitory effect of (-)-epigallocatechin-3-gallate on lipid accumulation of 3T3-L1 cells","volume":"15","author":"HS Moon","year":"2007","journal-title":"Obesity (Silver Spring)"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1006\/bbrc.2001.5484","article-title":"The effects of AICAR on adipocyte differentiation of 3T3-L1 cells","volume":"286","author":"SA Habinowski","year":"2001","journal-title":"Biochem Biophys Res Commun"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1248\/bpb.31.1716","article-title":"Effects of an AMP-activated protein kinase inhibitor, compound C, on adipogenic differentiation of 3T3-L1 cells","volume":"31","author":"Y Gao","year":"2008","journal-title":"Biol Pharm Bull"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"E175","DOI":"10.1152\/ajpendo.00453.2005","article-title":"Thiazolidinediones can rapidly activate AMP-activated protein kinase in mammalian tissues","volume":"291","author":"NK LeBrasseur","year":"2006","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"8839","DOI":"10.1074\/jbc.M111.263434","article-title":"Dehydrodiconiferyl alcohol isolated from Cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3T3-L1 cells and primary mouse embryonic fibroblasts","volume":"287","author":"J Lee","year":"2012","journal-title":"J Biol Chem"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1006\/bbrc.1997.7627","article-title":"CAAT\/enhancer binding proteins directly modulate transcription from the peroxisome proliferator-activated receptor gamma 2 promoter","volume":"240","author":"SL Clarke","year":"1997","journal-title":"Biochem Biophys Res Commun"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0125667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,24]],"date-time":"2019-08-24T06:50:47Z","timestamp":1566629447000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0125667"}},"subtitle":[],"editor":[{"given":"Reshma","family":"Taneja","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,4,30]]},"references-count":38,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2015,4,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0125667","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,30]]}}}