{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T05:47:17Z","timestamp":1769752037499,"version":"3.49.0"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T00:00:00Z","timestamp":1431907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0126239","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T20:17:41Z","timestamp":1431980261000},"page":"e0126239","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["Novel Heparan Sulfate-Binding Peptides for Blocking Herpesvirus Entry"],"prefix":"10.1371","volume":"10","author":[{"given":"Pranay","family":"Dogra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily B.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael L.","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James S.","family":"Foster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Hackenback","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen J.","family":"Kennel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim E.","family":"Sparer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan S.","family":"Wall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,5,18]]},"reference":[{"key":"ref1","first-page":"1960","article-title":"Fields Virology","author":"ES Mocarski","year":"2013"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1128\/CMR.00034-08","article-title":"Immunobiology of human cytomegalovirus: from bench to bedside","volume":"22","author":"T Crough","year":"2009","journal-title":"Clinical microbiology reviews"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.humimm.2004.02.009","article-title":"Pathogenesis of human cytomegalovirus infection and cellular targets","volume":"65","author":"G Gerna","year":"2004","journal-title":"Human immunology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"501801","DOI":"10.1155\/2013\/501801","article-title":"Cytomegalovirus in the neonate: immune correlates of infection and protection","volume":"2013","author":"MR Schleiss","year":"2013","journal-title":"Clinical & developmental immunology"},{"issue":"Suppl 2","key":"ref5","doi-asserted-by":"crossref","first-page":"B197","DOI":"10.1016\/j.vaccine.2012.10.074","article-title":"Desirability and feasibility of a vaccine against cytomegalovirus","volume":"31","author":"P Griffiths","year":"2013","journal-title":"Vaccine"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1586\/erv.10.125","article-title":"Update on the current status of cytomegalovirus vaccines","volume":"9","author":"H Sung","year":"2010","journal-title":"Expert review of vaccines"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"260","DOI":"10.3947\/ic.2013.45.3.260","article-title":"Cytomegalovirus infections in solid organ transplantation: a review","volume":"45","author":"P Ramanan","year":"2013","journal-title":"Infection & chemotherapy"},{"issue":"2\u20133","key":"ref8","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.antiviral.2006.05.002","article-title":"Antiviral drugs for cytomegalovirus diseases","volume":"71","author":"K Biron","year":"2006","journal-title":"Antiviral research"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1086\/519941","article-title":"Incidence and clinical features of ganciclovir-resistant cytomegalovirus disease in heart transplant recipients. Clinical infectious diseases: an official publication of the","volume":"45","author":"F Li","year":"2007","journal-title":"Infectious Diseases Society of America"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1128\/CMR.00009-10","article-title":"Antiviral drug resistance of human cytomegalovirus","volume":"23","author":"NS Lurain","year":"2010","journal-title":"Clinical microbiology reviews"},{"issue":"7","key":"ref11","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1093\/cid\/cis1035","article-title":"Clinical aspects of cytomegalovirus antiviral resistance in solid organ transplant recipients","volume":"56","author":"AK Le Page","year":"2013","journal-title":"Clinical infectious diseases: an official publication of the Infectious Diseases Society of America"},{"issue":"9230","key":"ref12","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/S0140-6736(00)02607-6","article-title":"Emergence of ganciclovir-resistant cytomegalovirus disease among recipients of solid-organ transplants","volume":"356","author":"AP Limaye","year":"2000","journal-title":"Lancet"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1006\/viro.1993.1192","article-title":"Initiation of human cytomegalovirus infection requires initial interaction with cell surface heparan sulfate","volume":"193","author":"T Compton","year":"1993","journal-title":"Virology"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.coviro.2012.01.001","article-title":"Human cytomegalovirus entry into cells","volume":"2","author":"AL Vanarsdall","year":"2012","journal-title":"Current opinion in virology"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1172\/JCI200113530","article-title":"Molecular diversity of heparan sulfate","volume":"108","author":"J Esko","year":"2001","journal-title":"The Journal of clinical investigation"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/S1367-5931(00)00145-9","article-title":"Heparin and heparan sulfate: biosynthesis, structure and function","volume":"4","author":"R Sasisekharan","year":"2000","journal-title":"Current opinion in chemical biology"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1039\/b100916h","article-title":"Heparin and heparan sulfate: structure and function","volume":"19","author":"DL Rabenstein","year":"2002","journal-title":"Natural product reports"},{"issue":"1","key":"ref18","first-page":"729777","article-title":"Functions of Cell Surface Heparan Sulfate Proteoglycans","volume":"68","author":"B Merton","year":"1999","journal-title":"Annual Review of Biochemistry"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1099\/00222615-48-3-223","article-title":"Glycosaminoglycan-binding microbial proteins in tissue adhesion and invasion: key events in microbial pathogenicity","volume":"48","author":"T Wadstrom","year":"1999","journal-title":"Journal of medical microbiology"},{"issue":"9","key":"ref20","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1096\/fj.03-1040fje","article-title":"Glypican-1 as an Abeta binding HSPG in the human brain: its localization in DIG domains and possible roles in the pathogenesis of Alzheimer's disease","volume":"18","author":"N Watanabe","year":"2004","journal-title":"FASEB journal: official publication of the Federation of American Societies for Experimental Biology"},{"issue":"5","key":"ref21","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1083\/jcb.116.5.1273","article-title":"Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans","volume":"116","author":"T Shieh M-","year":"1992","journal-title":"The Journal of Cell Biology"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1172\/JCI200113799","article-title":"Herpesviruses and heparan sulfate: an intimate relationship in aid of viral entry","volume":"108","author":"D Shukla","year":"2001","journal-title":"The Journal of clinical investigation"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/med.1026","article-title":"Cell surface heparan sulfate and its roles in assisting viral infections","volume":"22","author":"J Liu","year":"2002","journal-title":"Medicinal research reviews"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.bbrc.2013.05.063","article-title":"Peptide p5 binds both heparinase-sensitive glycosaminoglycans and fibrils in patient-derived AL amyloid extracts","volume":"436","author":"EB Martin","year":"2013","journal-title":"Biochemical and biophysical research communications"},{"issue":"34","key":"ref25","doi-asserted-by":"crossref","first-page":"E586","DOI":"10.1073\/pnas.1103247108","article-title":"In vivo molecular imaging of peripheral amyloidosis using heparin-binding peptides","volume":"108","author":"JS Wall","year":"2011","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1007\/s11307-011-0524-0","article-title":"Comparative analysis of peptide p5 and serum amyloid P component for imaging AA amyloid in mice using dual-isotope SPECT","volume":"14","author":"JS Wall","year":"2012","journal-title":"Molecular imaging and biology: MIB: the official publication of the Academy of Molecular Imaging"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.nucmedbio.2011.06.007","article-title":"SPECT imaging of peripheral amyloid in mice by targeting hyper-sulfated heparan sulfate proteoglycans with specific scFv antibodies","volume":"39","author":"JS Wall","year":"2012","journal-title":"Nucl Med Biol"},{"issue":"52","key":"ref28","doi-asserted-by":"crossref","first-page":"41143","DOI":"10.1074\/jbc.M110.153791","article-title":"The heparan sulfate motif (GlcNS6S-IdoA2S)3, common in heparin, has a strict topography and is involved in cell behavior and disease","volume":"285","author":"NC Smits","year":"2010","journal-title":"The Journal of biological chemistry"},{"issue":"12B","key":"ref29","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1101\/gad.5.12b.2375","article-title":"p53 alteration is a common event in the spontaneous immortalization of primary BALB\/c murine embryo fibroblasts","volume":"5","author":"DM Harvey","year":"1991","journal-title":"Genes & development"},{"issue":"20","key":"ref30","doi-asserted-by":"crossref","first-page":"9966","DOI":"10.1128\/JVI.75.20.9966-9976.2001","article-title":"Murine cytomegalovirus CC chemokine homolog MCK-2 (m131-129) is a determinant of dissemination that increases inflammation at initial sites of infection","volume":"75","author":"N Saederup","year":"2001","journal-title":"Journal of virology"},{"issue":"11","key":"ref31","doi-asserted-by":"crossref","first-page":"e50524","DOI":"10.1371\/journal.pone.0050524","article-title":"US28 is a potent activator of phospholipase C during HCMV infection of clinically relevant target cells","volume":"7","author":"WE Miller","year":"2012","journal-title":"PloS one"},{"issue":"8","key":"ref32","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.1128\/JVI.02442-10","article-title":"Human cytomegalovirus pUS27 G protein-coupled receptor homologue is required for efficient spread by the extracellular route but not for direct cell-to-cell spread","volume":"85","author":"CM O'Connor","year":"2011","journal-title":"Journal of virology"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1128\/JVI.79.5.2754-2767.2005","article-title":"Human cytomegalovirus labeled with green fluorescent protein for live analysis of intracellular particle movements","volume":"79","author":"KL Sampaio","year":"2005","journal-title":"Journal of virology"},{"issue":"9","key":"ref34","doi-asserted-by":"crossref","first-page":"e1002905","DOI":"10.1371\/journal.ppat.1002905","article-title":"PDGF receptor-alpha does not promote HCMV entry into epithelial and endothelial cells but increased quantities stimulate entry by an abnormal pathway","volume":"8","author":"AL Vanarsdall","year":"2012","journal-title":"PLoS pathogens"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1146\/annurev-cellbio-100913-013303","article-title":"Basic statistics in cell biology","volume":"30","author":"DL Vaux","year":"2014","journal-title":"Annual review of cell and developmental biology"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1038\/embor.2012.36","article-title":"Replicates and repeats\u2014what is the difference and is it significant? A brief discussion of statistics and experimental design","volume":"13","author":"DL Vaux","year":"2012","journal-title":"EMBO reports"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1471-2105-9-40","article-title":"I-TASSER server for protein 3D structure prediction","volume":"9","author":"Y Zhang","year":"2008","journal-title":"BMC bioinformatics"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nprot.2010.5","article-title":"I-TASSER: a unified platform for automated protein structure and function prediction","volume":"5","author":"A Roy","year":"2010","journal-title":"Nature protocols"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1002\/bip.21339","article-title":"Importance of the spatial display of charged residues in heparin-peptide interactions","volume":"93","author":"A Rullo","year":"2010","journal-title":"Biopolymers"},{"issue":"10","key":"ref40","doi-asserted-by":"crossref","first-page":"4751","DOI":"10.1128\/AAC.00854-13","article-title":"A peptide inhibitor of cytomegalovirus infection from human hemofiltrate","volume":"57","author":"EM Borst","year":"2013","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"28","key":"ref41","doi-asserted-by":"crossref","first-page":"25406","DOI":"10.1074\/jbc.M110.201103","article-title":"Anti-heparan sulfate peptides that block herpes simplex virus infection in vivo","volume":"286","author":"V Tiwari","year":"2011","journal-title":"The Journal of biological chemistry"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/S0014-5793(00)01964-5","article-title":"Binding of a de novo designed peptide to specific glycosaminoglycans","volume":"482","author":"G Jayaraman","year":"2000","journal-title":"FEBS letters"},{"issue":"3","key":"ref43","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1080\/13506120500232010","article-title":"Formation of experimental murine AA amyloid fibrils in SAP-deficient mice: high resolution ultrastructural study","volume":"12","author":"S Inoue","year":"2005","journal-title":"Amyloid"},{"issue":"6","key":"ref44","doi-asserted-by":"crossref","first-page":"e66181","DOI":"10.1371\/journal.pone.0066181","article-title":"A binding-site barrier affects imaging efficiency of high affinity amyloid-reactive Peptide radiotracers in vivo","volume":"8","author":"JS Wall","year":"2013","journal-title":"PloS one"},{"issue":"12","key":"ref45","doi-asserted-by":"crossref","first-page":"6434","DOI":"10.1128\/JVI.00433-12","article-title":"A 3-O-sulfated heparan sulfate binding peptide preferentially targets herpes simplex virus 2-infected cells","volume":"86","author":"MM Ali","year":"2012","journal-title":"Journal of virology"},{"issue":"3","key":"ref46","doi-asserted-by":"crossref","first-page":"e57318","DOI":"10.1371\/journal.pone.0057318","article-title":"A novel cell-penetrating peptide derived from human eosinophil cationic protein","volume":"8","author":"SL Fang","year":"2013","journal-title":"PloS one"},{"issue":"37","key":"ref47","doi-asserted-by":"crossref","first-page":"35109","DOI":"10.1074\/jbc.M301726200","article-title":"Antennapedia and HIV transactivator of transcription (TAT) "protein transduction domains" promote endocytosis of high molecular weight cargo upon binding to cell surface glycosaminoglycans","volume":"278","author":"S Console","year":"2003","journal-title":"The Journal of biological chemistry"},{"issue":"4","key":"ref48","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/j.1600-0854.2007.00540.x","article-title":"Internalization and trafficking of cell surface proteoglycans and proteoglycan-binding ligands","volume":"8","author":"CK Payne","year":"2007","journal-title":"Traffic"},{"issue":"4","key":"ref49","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1038\/74464","article-title":"Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells","volume":"18","author":"M Lewin","year":"2000","journal-title":"Nature biotechnology"},{"issue":"7","key":"ref50","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/S0962-8924(00)01771-2","article-title":"Protein transduction: unrestricted delivery into all cells?","volume":"10","author":"SR Schwarze","year":"2000","journal-title":"Trends Cell Biol"},{"issue":"4","key":"ref51","first-page":"807","article-title":"Development and evaluation of agents for targeting visceral amyloid","volume":"33","author":"JS Wall","year":"2011","journal-title":"Tijdschrift voor nu[c]leaire geneeskunde"},{"issue":"3","key":"ref52","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1128\/JVI.02979-14","article-title":"Characterization of a proteolytically stable d-Peptide that suppresses herpes simplex virus 1 infection: implications for the development of entry-based antiviral therapy","volume":"89","author":"D Jaishankar","year":"2015","journal-title":"Journal of virology"},{"issue":"6","key":"ref53","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.1128\/JVI.70.6.3461-3469.1996","article-title":"Differences in the susceptibility of herpes simplex virus types 1 and 2 to modified heparin compounds suggest serotype differences in viral entry","volume":"70","author":"BC Herold","year":"1996","journal-title":"Journal of virology"},{"issue":"29","key":"ref54","doi-asserted-by":"crossref","first-page":"18881","DOI":"10.1016\/S0021-9258(17)32250-0","article-title":"Cell surface syndecan-1 on distinct cell types differs in fine structure and ligand binding of its heparan sulfate chains","volume":"269","author":"M Kato","year":"1994","journal-title":"The Journal of biological chemistry"},{"issue":"39","key":"ref55","doi-asserted-by":"crossref","first-page":"24979","DOI":"10.1074\/jbc.273.39.24979","article-title":"Regulated diversity of heparan sulfate","volume":"273","author":"U Lindahl","year":"1998","journal-title":"The Journal of biological chemistry"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0126239","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T12:29:19Z","timestamp":1652012959000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0126239"}},"subtitle":[],"editor":[{"given":"Michael","family":"Nevels","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,18]]},"references-count":55,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0126239","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,18]]}}}