{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T06:15:31Z","timestamp":1775110531321,"version":"3.50.1"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T00:00:00Z","timestamp":1432684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0126352","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T18:07:08Z","timestamp":1432750028000},"page":"e0126352","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":47,"title":["C-Terminal Clostridium perfringens Enterotoxin-Mediated Antigen Delivery for Nasal Pneumococcal Vaccine"],"prefix":"10.1371","volume":"10","author":[{"given":"Hidehiko","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Watari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eri","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Yonemitsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kiyono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyohito","family":"Yagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masuo","family":"Kondoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Kunisawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,5,27]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1586\/erv.11.196","article-title":"Novel vaccine development strategies for inducing mucosal immunity","volume":"11","author":"Y Fujkuyama","year":"2012","journal-title":"Expert Review of Vaccines"},{"issue":"1","key":"ref2","first-page":"1","article-title":"Mucosal vaccination\u2014an old but still vital strategy","volume":"58","author":"H Dlugonska","year":"2012","journal-title":"Annals of Parasitology"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/mi.2013.30","article-title":"Microfold (M) cells: important immunosurveillance posts in the intestinal epithelium","volume":"6","author":"NA Mabbott","year":"2013","journal-title":"Mucosal Immunol"},{"issue":"30","key":"ref4","doi-asserted-by":"crossref","first-page":"5467","DOI":"10.1016\/j.vaccine.2006.12.001","article-title":"Induction of secretory immunity and memory at mucosal surfaces","volume":"25","author":"P Brandtzaeg","year":"2007","journal-title":"Vaccine"},{"issue":"6","key":"ref5","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.addr.2011.07.003","article-title":"Gut-associated lymphoid tissues for the development of oral vaccines","volume":"64","author":"J Kunisawa","year":"2012","journal-title":"Advanced Drug Delivery Reviews"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.it.2008.07.008","article-title":"Immunological commonalities and distinctions between airway and digestive immunity","volume":"29","author":"J Kunisawa","year":"2008","journal-title":"Trends Immunol"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"2789","DOI":"10.1084\/jem.20070607","article-title":"A novel M cell-specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses","volume":"204","author":"T Nochi","year":"2007","journal-title":"J Exp Med"},{"issue":"11","key":"ref8","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1093\/intimm\/dxu061","article-title":"A novel mucosal vaccine targeting Peyer&apos;s patch M cells induces protective antigen-specific IgA responses","volume":"26","author":"H Shima","year":"2014","journal-title":"Int Immunol"},{"issue":"9","key":"ref9","doi-asserted-by":"crossref","first-page":"4717","DOI":"10.4049\/jimmunol.171.9.4717","article-title":"M cell DNA vaccination for CTL immunity to HIV","volume":"171","author":"X Wang","year":"2003","journal-title":"J Immunol"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"a002907","DOI":"10.1101\/cshperspect.a002907","article-title":"Molecular basis of the core structure of tight junctions","volume":"2","author":"M Furuse","year":"2010","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"20","key":"ref11","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1016\/j.biomaterials.2010.03.047","article-title":"Mucosal vaccination using claudin-4-targeting","volume":"31","author":"H Kakutani","year":"2010","journal-title":"Biomaterials"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1083\/jcb.147.1.195","article-title":"<italic>Clostridium perfringens<\/italic> enterotoxin fragment removes specific claudins from tight junction strands: Evidence for direct involvement of claudins in tight junction barrier","volume":"147","author":"N Sonoda","year":"1999","journal-title":"J Cell Biol"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1074\/jbc.M708066200","article-title":"Structure of the claudin-binding domain of <italic>Clostridium perfringens<\/italic> enterotoxin","volume":"283","author":"CM Van Itallie","year":"2008","journal-title":"J Biol Chem"},{"issue":"10","key":"ref14","first-page":"766","article-title":"The safety of a mucosal vaccine using the C-terminal fragment of <italic>Clostridium perfringens<\/italic> enterotoxin","volume":"65","author":"H Suzuki","year":"2010","journal-title":"Pharmazie"},{"issue":"6","key":"ref15","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.ijporl.2010.03.049","article-title":"Challenges in reducing the burden of otitis media disease: an ENT perspective on improving management and prospects for prevention","volume":"74","author":"G Grevers","year":"2010","journal-title":"Int J Pediatr Otorhinolaryngol"},{"issue":"18","key":"ref16","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1007\/s00018-012-1234-8","article-title":"Serotype-independent pneumococcal vaccines","volume":"70","author":"EN Miyaji","year":"2013","journal-title":"Cell Mol Life Sci"},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1128\/JCM.02199-06","article-title":"Discovery of a new capsular serotype (6C) within serogroup 6 of <italic>Streptococcus pneumoniae<\/italic>","volume":"45","author":"IH Park","year":"2007","journal-title":"J Clin Microbiol"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1086\/653123","article-title":"A new pneumococcal serotype, 11E, has a variably inactivated wcjE gene","volume":"202","author":"JJ Calix","year":"2010","journal-title":"J Infect Dis"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.ijporl.2013.12.028","article-title":"Impact of 13-valent pneumococcal conjugate vaccine on otitis media bacteriology","volume":"78","author":"AS Zhao","year":"2014","journal-title":"Int J Pediatr Otorhinolaryngol"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Harboe ZB, Dalby T, Weinberger DM, Benfield T, Molbak K, Slotved HC, et al. Impact of 13-Valent Pneumococcal Conjugate Vaccination in Invasive Pneumococcal Disease Incidence and Mortality. Clin Infect Dis. 2014.","DOI":"10.1093\/cid\/ciu524"},{"issue":"9","key":"ref21","doi-asserted-by":"crossref","first-page":"5031","DOI":"10.1128\/IAI.72.9.5031-5040.2004","article-title":"PspA protects <italic>Streptococcus pneumoniae<\/italic> from killing by apolactoferrin, and antibody to PspA enhances killing of pneumococci by apolactoferrin","volume":"72","author":"M Shaper","year":"2004","journal-title":"Infect Immun"},{"issue":"10","key":"ref22","doi-asserted-by":"crossref","first-page":"3293","DOI":"10.1128\/IAI.58.10.3293-3299.1990","article-title":"Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular serotypes of <italic>Streptococcus pneumoniae<\/italic>","volume":"58","author":"MJ Crain","year":"1990","journal-title":"Infect Immun"},{"issue":"34","key":"ref23","doi-asserted-by":"crossref","first-page":"5731","DOI":"10.1016\/j.vaccine.2011.05.095","article-title":"Intranasal immunization with recombinant PspA fused with a flagellin enhances cross-protective immunity against <italic>Streptococcus pneumoniae<\/italic> infection in mice","volume":"29","author":"CT Nguyen","year":"2011","journal-title":"Vaccine"},{"issue":"17","key":"ref24","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1016\/S0264-410X(99)00530-7","article-title":"Immunization of healthy adults with a single recombinant pneumococcal surface protein A (PspA) variant stimulates broadly cross-reactive antibodies to heterologous PspA molecules","volume":"18","author":"GS Nabors","year":"2000","journal-title":"Vaccine"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1086\/314827","article-title":"Nasal immunization induces <italic>Haemophilus influenzae<\/italic>-specific Th1 and Th2 responses with mucosal IgA and systemic IgG antibodies for protective immunity","volume":"180","author":"Y Kurono","year":"1999","journal-title":"J Infect Dis"},{"issue":"5","key":"ref26","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1128\/IAI.00240-13","article-title":"Nanogel-based PspA intranasal vaccine prevents invasive disease and nasal colonization by <italic>Streptococcus pneumoniae<\/italic>","volume":"81","author":"IG Kong","year":"2013","journal-title":"Infect Immun"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jconrel.2005.07.008","article-title":"Role of C-terminal regions of the C-terminal fragment of <italic>Clostridium perfringens<\/italic> enterotoxin in its interaction with claudin-4","volume":"108","author":"A Takahashi","year":"2005","journal-title":"J Control Release"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.jconrel.2009.10.033","article-title":"Claudin 4-targeted protein incorporated into PLGA nanoparticles can mediate M cell targeted delivery","volume":"142","author":"TE Rajapaksa","year":"2010","journal-title":"J Control Release"},{"issue":"7","key":"ref29","first-page":"1899","article-title":"Cellular effectors mediating Th17-dependent clearance of pneumococcal colonization in mice","volume":"119","author":"Z Zhang","year":"2009","journal-title":"J Clin Invest"},{"issue":"8","key":"ref30","doi-asserted-by":"crossref","first-page":"3758","DOI":"10.1128\/IAI.66.8.3758-3766.1998","article-title":"Interactions of the invasive pathogens <italic>Salmonella typhimurium<\/italic>, <italic>Listeria monocytogenes<\/italic>, and <italic>Shigella flexneri<\/italic> with M cells and murine Peyer&apos;s patches","volume":"66","author":"VB Jensen","year":"1998","journal-title":"Infect Immun"},{"issue":"3","key":"ref31","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1128\/IAI.66.3.1237-1243.1998","article-title":"M-cell surface\u03b21 integrin expression and invasin-mediated targeting of <italic>Yersinia pseudotuberculosis<\/italic> to mouse Peyer&apos;s patch M cells","volume":"66","author":"MA Clark","year":"1998","journal-title":"Infect Immun"},{"issue":"7270","key":"ref32","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nature08529","article-title":"Uptake through glycoprotein 2 of FimH<sup>+<\/sup>bacteria by M cells initiates mucosal immune response","volume":"462","author":"K Hase","year":"2009","journal-title":"Nature"},{"issue":"14","key":"ref33","doi-asserted-by":"crossref","first-page":"7964","DOI":"10.1128\/JVI.77.14.7964-7977.2003","article-title":"The viral\u03c31 protein and glycoconjugates containing \u03b12-3-linked sialic acid are involved in type 1 reovirus adherence to M cell apical surfaces","volume":"77","author":"A Helander","year":"2003","journal-title":"J Virol"},{"issue":"5","key":"ref34","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1128\/IAI.01737-06","article-title":"Identification of a prepore large-complex stage in the mechanism of action of <italic>Clostridium perfringens<\/italic> enterotoxin","volume":"75","author":"JG Smedley 3rd","year":"2007","journal-title":"Infect Immun"},{"issue":"3","key":"ref35","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1124\/mol.104.008375","article-title":"A novel strategy for the enhancement of drug absorption using a claudin modulator","volume":"67","author":"M Kondoh","year":"2005","journal-title":"Mol Pharmacol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.biochem.72.121801.161800","article-title":"Signals for sorting of transmembrane proteins to endosomes and lysosomes","volume":"72","author":"JS Bonifacino","year":"2003","journal-title":"Annu Rev Biochem"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1091\/mbc.E03-05-0319","article-title":"Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment","volume":"15","author":"AI Ivanov","year":"2004","journal-title":"Mol Biol Cell"},{"issue":"17","key":"ref38","doi-asserted-by":"crossref","first-page":"11037","DOI":"10.1016\/S0021-9258(18)99124-6","article-title":"Localization of the receptor-binding region of <italic>Clostridium perfringens<\/italic> enterotoxin utilizing cloned toxin fragments and synthetic peptides. The 30 C-terminal amino acids define a functional binding region","volume":"266","author":"PC Hanna","year":"1991","journal-title":"J Biol Chem"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1128\/IAI.65.3.1014-1022.1997","article-title":"Deletion analysis of the <italic>Clostridium perfringens<\/italic> enterotoxin","volume":"65","author":"JF Kokai-Kun","year":"1997","journal-title":"Infect Immun"},{"issue":"7","key":"ref40","first-page":"543","article-title":"A toxicological evaluation of a claudin modulator, the C-terminal fragment of <italic>Clostridium perfringens<\/italic> enterotoxin, in mice","volume":"66","author":"H Suzuki","year":"2011","journal-title":"Pharmazie"},{"issue":"6","key":"ref41","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1086\/317602","article-title":"Immunization of humans with recombinant pneumococcal surface protein A (rPspA) elicits antibodies that passively protect mice from fatal infection with <italic>Streptococcus pneumoniae<\/italic> bearing heterologous PspA","volume":"182","author":"DE Briles","year":"2000","journal-title":"J Infect Dis"},{"issue":"3","key":"ref42","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1128\/CVI.00430-09","article-title":"Immunization of mice with single PspA fragments induces antibodies capable of mediating complement deposition on different pneumococcal strains and cross-protection","volume":"17","author":"AT Moreno","year":"2010","journal-title":"Clin Vaccine Immunol"},{"issue":"3","key":"ref43","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.4049\/jimmunol.1000831","article-title":"Secretory-IgA antibodies play an important role in the immunity to <italic>Streptococcus pneumoniae<\/italic>","volume":"185","author":"Y Fukuyama","year":"2010","journal-title":"J Immunol"},{"issue":"9","key":"ref44","doi-asserted-by":"crossref","first-page":"4720","DOI":"10.1128\/IAI.67.9.4720-4724.1999","article-title":"Pneumococcal surface protein A inhibits complement activation by <italic>Streptococcus pneumoniae<\/italic>","volume":"67","author":"AH Tu","year":"1999","journal-title":"Infect Immun"},{"issue":"4","key":"ref45","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1128\/IAI.01384-06","article-title":"Contributions of pneumolysin, pneumococcal surface protein A (PspA), and PspC to pathogenicity of <italic>Streptococcus pneumoniae<\/italic> D39 in a mouse model","volume":"75","author":"AD Ogunniyi","year":"2007","journal-title":"Infect Immun"},{"issue":"8","key":"ref46","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1016\/j.vaccine.2010.12.074","article-title":"Selection of family 1 PspA molecules capable of inducing broad-ranging cross-reactivity by complement deposition and opsonophagocytosis by murine peritoneal cells","volume":"29","author":"C Goulart","year":"2011","journal-title":"Vaccine"},{"issue":"3","key":"ref47","doi-asserted-by":"crossref","first-page":"e59605","DOI":"10.1371\/journal.pone.0059605","article-title":"Characterization of protective immune responses induced by pneumococcal surface protein A in fusion with pneumolysin derivatives","volume":"8","author":"C Goulart","year":"2013","journal-title":"PLoS One"},{"issue":"2","key":"ref48","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1128\/IAI.68.2.796-800.2000","article-title":"Intranasal immunization of mice with a mixture of the pneumococcal proteins PsaA and PspA is highly protective against nasopharyngeal carriage of <italic>Streptococcus pneumoniae<\/italic>","volume":"68","author":"DE Briles","year":"2000","journal-title":"Infect Immun"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0126352","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T17:17:10Z","timestamp":1652116630000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0126352"}},"subtitle":[],"editor":[{"given":"Eliane Namie","family":"Miyaji","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,27]]},"references-count":48,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0126352","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,27]]}}}