{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:08:31Z","timestamp":1793383711041,"version":"4.5.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T00:00:00Z","timestamp":1433980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0126479","type":"journal-article","created":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T13:53:14Z","timestamp":1434030794000},"page":"e0126479","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":91,"title":["Three Dimensional Checkerboard Synergy Analysis of Colistin, Meropenem, Tigecycline against Multidrug-Resistant Clinical Klebsiella pneumonia Isolates"],"prefix":"10.1371","volume":"10","author":[{"given":"Claudia","family":"Stein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliwia","family":"Makarewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen A.","family":"Bohnert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Pfeifer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Kesselmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hagel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias W.","family":"Pletz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,6,11]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1128\/CMR.00001-07","article-title":"Carbapenemases: the versatile beta-lactamases","volume":"20","author":"AM Queenan","year":"2007","journal-title":"Clin Microbiol Rev"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1128\/AAC.45.4.1151-1161.2001","article-title":"Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae","volume":"45","author":"H Yigit","year":"2001","journal-title":"Antimicrobial agents and chemotherapy"},{"key":"ref3","article-title":"Annual epidemiological report 2013: Reporting on 2011 surveillance data and 2012 epidemic intelligence data","year":"2013"},{"issue":"10","key":"ref4","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1086\/605922","article-title":"Attributable mortality rate for carbapenem-resistant Klebsiella pneumoniae bacteremia","volume":"30","author":"A Borer","year":"2009","journal-title":"Infection control and hospital epidemiology: the official journal of the Society of Hospital Epidemiologists of America"},{"issue":"12","key":"ref5","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1086\/592412","article-title":"Outcomes of carbapenem-resistant Klebsiella pneumoniae infection and the impact of antimicrobial and adjunctive therapies","volume":"29","author":"G Patel","year":"2008","journal-title":"Infect Control Hosp Epidemiol"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3201\/eid1710.110655","article-title":"Global spread of Carbapenemase-producing Enterobacteriaceae","volume":"17","author":"P Nordmann","year":"2011","journal-title":"Emerging infectious diseases"},{"issue":"10","key":"ref7","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/AAC.47.10.3332-3335.2003","article-title":"Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance","volume":"47","author":"A Domenech-Sanchez","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"ref8","doi-asserted-by":"crossref","first-page":"4178","DOI":"10.1128\/AAC.01301-09","article-title":"An ertapenem-resistant extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae clone carries a novel OmpK36 porin variant","volume":"54","author":"A Garcia-Fernandez","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"ref9","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1007\/s10096-012-1665-z","article-title":"Spread of an OmpK36-modified ST15 Klebsiella pneumoniae variant during an outbreak involving multiple carbapenem-resistant Enterobacteriaceae species and clones","volume":"31","author":"A Novais","year":"2012","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"10","key":"ref10","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1128\/JCM.01244-13","article-title":"Outbreak caused by an ertapenem-resistant, CTX-M-15-producing Klebsiella pneumoniae sequence type 101 clone carrying an OmpK36 porin variant","volume":"51","author":"A Poulou","year":"2013","journal-title":"J Clin Microbiol"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1128\/AAC.06268-11","article-title":"Treatment outcome of bacteremia due to KPC-producing Klebsiella pneumoniae: superiority of combination antimicrobial regimens","volume":"56","author":"ZA Qureshi","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"ref12","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1093\/cid\/cis588","article-title":"Predictors of mortality in bloodstream infections caused by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae: importance of combination therapy","volume":"55","author":"M Tumbarello","year":"2012","journal-title":"Clin Infect Dis"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"3541","DOI":"10.1128\/AAC.02449-14","article-title":"In vitro and in vivo activities of tigecycline-colistin combination therapies against carbapenem-resistant Enterobacteriaceae","volume":"58","author":"JW Betts","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1128\/AAC.42.4.744","article-title":"Use of pharmacodynamic parameters to predict efficacy of combination therapy by using fractional inhibitory concentration kinetics","volume":"42","author":"JG den Hollander","year":"1998","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1093\/jac\/dkm215","article-title":"Collateral damage of flomoxef therapy: in vivo development of porin deficiency and acquisition of blaDHA-1 leading to ertapenem resistance in a clinical isolate of Klebsiella pneumoniae producing CTX-M-3 and SHV-5 beta-lactamases","volume":"60","author":"CH Lee","year":"2007","journal-title":"J Antimicrob Chemother"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.bmc.2012.10.053","article-title":"Search for new tools to combat Gram-negative resistant bacteria among amine derivatives of 5-arylidenehydantoin","volume":"21","author":"J Handzlik","year":"2013","journal-title":"Bioorg Med Chem"},{"issue":"14","key":"ref17","doi-asserted-by":"crossref","first-page":"4245","DOI":"10.1016\/j.bmc.2012.05.064","article-title":"Synthesis and SAR-study for novel arylpiperazine derivatives of 5-arylidenehydantoin with alpha(1)-adrenoceptor antagonistic properties","volume":"20","author":"J Handzlik","year":"2012","journal-title":"Bioorg Med Chem"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1128\/IAI.63.3.903-910.1995","article-title":"A porin from Klebsiella pneumoniae: sequence homology, three-dimensional model, and complement binding","volume":"63","author":"S Alberti","year":"1995","journal-title":"Infect Immun"},{"issue":"11","key":"ref19","doi-asserted-by":"crossref","first-page":"5258","DOI":"10.1128\/AAC.01069-13","article-title":"Mutations of the ompK36 porin gene and promoter impact responses of sequence type 258, KPC-2-producing Klebsiella pneumoniae strains to doripenem and doripenem-colistin","volume":"57","author":"CJ Clancy","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"ref20","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1128\/AAC.00620-10","article-title":"Optimized Nile Red efflux assay of AcrAB-TolC multidrug efflux system shows competition between substrates","volume":"54","author":"JA Bohnert","year":"2010","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"3","key":"ref21","first-page":"301","article-title":"Antibacterial effects of water-soluble low-molecular-weight chitosans on different microorganisms","volume":"40","author":"DV Gerasimenko","year":"2004","journal-title":"Prikl Biokhim Mikrobiol"},{"issue":"9","key":"ref22","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1099\/jmm.0.008094-0","article-title":"Reduced susceptibility to carbapenems in Klebsiella pneumoniae clinical isolates associated with plasmid-mediated beta-lactamase production and OmpK36 porin deficiency","volume":"58","author":"XD Wang","year":"2009","journal-title":"J Med Microbiol"},{"issue":"11","key":"ref23","doi-asserted-by":"crossref","first-page":"e79640","DOI":"10.1371\/journal.pone.0079640","article-title":"Single or in combination antimicrobial resistance mechanisms of Klebsiella pneumoniae contribute to varied susceptibility to different carbapenems","volume":"8","author":"YK Tsai","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1469-0691.2007.01860.x","article-title":"Extended-spectrum beta-lactamases and the permeability barrier","volume":"14","author":"L Martinez-Martinez","year":"2008","journal-title":"Clin Microbiol Infect"},{"issue":"4","key":"ref25","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1128\/AAC.01441-10","article-title":"Role of Klebsiella pneumoniae LamB Porin in antimicrobial resistance","volume":"55","author":"L Garcia-Sureda","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"ref26","doi-asserted-by":"crossref","first-page":"3396","DOI":"10.1128\/AAC.00285-06","article-title":"High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of bla(ACT-1) beta-lactamase production, porin OmpK35\/36 insertional inactivation, and down-regulation of the phosphate transport porin phoe","volume":"50","author":"FM Kaczmarek","year":"2006","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"5","key":"ref27","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1128\/AAC.02411-12","article-title":"Characterization of porin expression in Klebsiella pneumoniae Carbapenemase (KPC)-producing K. pneumoniae identifies isolates most susceptible to the combination of colistin and carbapenems","volume":"57","author":"JH Hong","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"ref28","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1093\/cid\/cis588","article-title":"Predictors of mortality in bloodstream infections caused by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae: importance of combination therapy","volume":"55","author":"M Tumbarello","year":"2012","journal-title":"Clin Infect Dis"},{"issue":"9 Supplement","key":"ref29","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1128\/JCM.00580-11","article-title":"The Clinical Predictive Value (or Lack Thereof) of the Results of In Vitro Antimicrobial Susceptibility Tests","volume":"49","author":"GV Doern","year":"2011","journal-title":"Journal of Clinical Microbiology"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.1016\/S0021-9258(17)41716-9","article-title":"Porins and specific diffusion channels in bacterial outer membranes","volume":"269","author":"H Nikaido","year":"1994","journal-title":"J Biol Chem"},{"issue":"4","key":"ref31","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1093\/jac\/dkh125","article-title":"Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA","volume":"53","author":"G Bauer","year":"2004","journal-title":"J Antimicrob Chemother"},{"issue":"5","key":"ref32","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1128\/AAC.2.5.331","article-title":"Locus of divalent cation inhibition of the bactericidal action of polymyxin B","volume":"2","author":"CC Chen","year":"1972","journal-title":"Antimicrob Agents Chemother"},{"issue":"8","key":"ref33","doi-asserted-by":"crossref","first-page":"3430","DOI":"10.1128\/AAC.01361-08","article-title":"Population pharmacokinetic analysis of colistin methanesulfonate and colistin after intravenous administration in critically ill patients with infections caused by gram-negative bacteria","volume":"53","author":"D Plachouras","year":"2009","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/j.1365-2125.2011.03991.x","article-title":"Neurotoxic effects associated with antibiotic use: management considerations","volume":"72","author":"MF Grill","year":"2011","journal-title":"Brit J Clin Pharmaco"},{"issue":"4","key":"ref35","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1128\/AAC.02166-13","article-title":"Carbapenemase-producing Klebsiella pneumoniae bloodstream infections: lowering mortality by antibiotic combination schemes and the role of carbapenems","volume":"58","author":"GL Daikos","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"ref36","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1093\/jac\/dku168","article-title":"Combination therapy for carbapenem-resistant Gram-negative bacteria","volume":"69","author":"M Paul","year":"2014","journal-title":"J Antimicrob Chemother"},{"issue":"4","key":"ref37","first-page":"405","article-title":"Is combination therapy for carbapenem-resistant Klebsiella pneumoniae the new standard of care?","volume":"13","author":"RR Watkins","year":"2015","journal-title":"Expert Review of Anti-infective Therapy"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0126479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T14:35:29Z","timestamp":1652366129000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0126479"}},"subtitle":[],"editor":[{"given":"Yung-Fu","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,6,11]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,6,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0126479","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,11]]}}}