{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:33:35Z","timestamp":1791304415209,"version":"4.3.1"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2015,7,8]],"date-time":"2015-07-08T00:00:00Z","timestamp":1436313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0127430","type":"journal-article","created":{"date-parts":[[2015,7,8]],"date-time":"2015-07-08T17:58:26Z","timestamp":1436378306000},"page":"e0127430","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":31,"title":["Use of a New High Resolution Melting Method for Genotyping Pathogenic Leptospira spp."],"prefix":"10.1371","volume":"10","author":[{"given":"Florence","family":"Naze","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Desvars","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Bourhy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Michault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,7,8]]},"reference":[{"key":"ref1","first-page":"287","article-title":"<italic>Leptospira<\/italic> and leptospirosis","volume":"140","author":"B Adler","year":"2010","journal-title":"VetMicrobiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: a zoonotic disease of global importance","volume":"3","author":"AR Bharti","year":"2003","journal-title":"Lancet Infectious Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1038\/nrmicro2208","article-title":"<italic>Leptospira<\/italic>: the dawn of the molecular genetics era for an emerging zoonotic pathogen","volume":"7","author":"AI Ko","year":"2009","journal-title":"Nat Rev Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.medmal.2012.11.005","article-title":"Diagnosis and epidemiology of leptospirosis","volume":"43","author":"M Picardeau","year":"2013","journal-title":"Med Mal Infect"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1099\/00207713-49-2-839","article-title":"Further determination of DNA relatedness between serogroups and serovars in the family <italic>Leptospiraceae<\/italic> with a proposal for <italic>Leptospira alexanderi<\/italic> sp. nov. and four new <italic>Leptospira<\/italic> genomospecies","volume":"49","author":"DJ Brenner","year":"1999","journal-title":"Int J Syst Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1128\/IAI.59.1.323-329.1991","article-title":"Pulsed-field gel elctrophoretic analysis of leptospiral DNA","volume":"59","author":"KA Taylor","year":"1991","journal-title":"Infect Immun"},{"key":"ref7","first-page":"192","article-title":"Use of restriction endonucleases for differentiation of pathogenic &amp; saprophytic leptospires","volume":"116","author":"J Shukla","year":"2002","journal-title":"Indian J Med Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.mimet.2007.01.012","article-title":"Assessment of Southern blot ribotyping for differentiation of Leptospira strains isolated from field rats","volume":"69","author":"U Kositanont","year":"2007","journal-title":"J Microbiol Methods"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1128\/JCM.31.11.2927-2932.1993","article-title":"Rapid identification of some <italic>Leptospira<\/italic> isolates from cattle by random amplified polymorphic DNA fingerprinting","volume":"31","author":"BG Corney","year":"1993","journal-title":"J Clin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1128\/JCM.31.3.578-583.1993","article-title":"Restriction fragment length polymorphisms distinguish <italic>Leptospira borgpetersenii<\/italic> serovar hardjo type hardjo-bovis isolates from different geographical locations","volume":"31","author":"RL Zuerner","year":"1993","journal-title":"J Clin Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"3575","DOI":"10.1128\/JCM.42.8.3575-3580.2004","article-title":"Use of fluorescent amplified fragment length polymorphism for molecular epidemiology of leptospirosis in India","volume":"42","author":"P Vijayachari","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref12","first-page":"3510","article-title":"Species-specific identification of <italic>Leptospiraceae<\/italic> by 16S rRNA gene sequencing","volume":"44","author":"RE Morey","year":"2006","journal-title":"J ClinMicrobiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1128\/JCM.43.2.539-545.2005","article-title":"Identification of variable-number tandem-repeat loci in <italic>Leptospira interrogans<\/italic> sensu stricto","volume":"43","author":"Z Majed","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1476-0711-5-28","article-title":"Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species","volume":"5","author":"N Ahmed","year":"2006","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1590\/S0074-02762012000500011","article-title":"A combined approach of VNTR and MLST analysis: improving molecular typing of Argentinean isolates of Leptospira interrogans","volume":"107","author":"K Caimi","year":"2012","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.tim.2003.08.006","article-title":"Multi-locus sequence typing: a tool for global epidemiology","volume":"11","author":"R Urwin","year":"2003","journal-title":"Trends Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1373\/49.3.396","article-title":"Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes","volume":"49","author":"CN Gundry","year":"2003","journal-title":"Clinl Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1373\/49.6.853","article-title":"High-resolution genotyping by amplicon melting analysis using LCGreen","volume":"49","author":"CT Wittwer","year":"2003","journal-title":"Clin Chem"},{"key":"ref19","first-page":"02021","article-title":"Rapid identification and discrimination of Brucella isolates using real-time PCR and high resolution melt analysis","author":"JM Winchell","year":"2010","journal-title":"J Clin Microbiol: JCM"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1111\/j.1365-2672.2009.04388.x","article-title":"Characterization of Chlamydiaceae species using PCR and high resolution melt curve analysis of the 16S rRNA gene","volume":"107","author":"T Robertson","year":"2009","journal-title":"J Appl Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"4117","DOI":"10.1128\/JCM.01696-09","article-title":"Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis","volume":"47","author":"SB Schwartz","year":"2009","journal-title":"JClin Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e581","DOI":"10.1371\/journal.pntd.0000581","article-title":"Detection and identification of old world Leishmania by high resolution melt analysis","volume":"4","author":"D Talmi-Frank","year":"2010","journal-title":"Plos Negl Trop Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.mimet.2009.03.007","article-title":"Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis","volume":"77","author":"WF Chan","year":"2009","journal-title":"J Microbiol Methods"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1373\/clinchem.2007.093658","article-title":"High-Resolution Melting analysis of the spa repeat region of <italic>Staphylococcus aureus<\/italic>","volume":"54","author":"AJ Stephens","year":"2008","journal-title":"Clin Chem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1373\/clinchem.2007.085993","article-title":"Optimization of high-resolution melting analysis for low-cost and rapid screening of allelic variants of Bacillus anthracis by multiple-locus variable-number tandem repeat analysis","volume":"53","author":"D Fortini","year":"2007","journal-title":"Clin Chem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1099\/mic.0.2006\/005140-0","article-title":"Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region","volume":"153","author":"N Jeffery","year":"2007","journal-title":"Microbiology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1128\/JCM.00402-10","article-title":"Pseudomonas aeruginosa outbreak linked to mineral water bottles in a neonatal intensive care unit: fast typing by use of high-resolution melting analysis of a variable-number tandem-repeat locus","volume":"48","author":"F Naze","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1128\/JCM.01567-08","article-title":"Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region","volume":"47","author":"PA Steer","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1373\/clinchem.2006.068510","article-title":"Multiplex single-color PCR with amplicon melting analysis for identification of Aspergillus species","volume":"52","author":"M Erali","year":"2006","journal-title":"Clin Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1186\/1475-2875-8-282","article-title":"Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group","volume":"8","author":"SB Vezenegho","year":"2009","journal-title":"Malar J"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1179\/136485910X12607012374037","article-title":"High-resolution melt-curve analysis of random-amplified-polymorphic-DNA markers, for the characterisation of pathogenic Leptospira","volume":"104","author":"SM Tulsiani","year":"2010","journal-title":"Ann Trop Med Parasitol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/1297-9716-44-80","article-title":"Leptospirosis in the western Indian Ocean islands: what is known so far?","volume":"44","author":"A Desvars","year":"2013","journal-title":"Vet Res"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"4061","DOI":"10.1099\/ijs.0.066597-0","article-title":"Leptospira mayottensis sp. nov., a pathogenic species of the genus Leptospira isolated from humans","volume":"64","author":"P Bourhy","year":"2014","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1099\/00221287-139-8-1691","article-title":"Detection of seven species of pathogenic leptospires by PCR using two sets of primers","volume":"139","author":"C Gravekamp","year":"1993","journal-title":"J Gen Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.femsle.2005.06.011","article-title":"A rapid and quantitative method for the detection of Leptospira species in human leptospirosis","volume":"249","author":"F Merien","year":"2005","journal-title":"FEMS Microbiol Lett"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e7093","DOI":"10.1371\/journal.pone.0007093","article-title":"Development and validation of a real-time PCR for detection of pathogenic leptospira species in clinical materials","volume":"4","author":"A Ahmed","year":"2009","journal-title":"PLoS ONE"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1099\/jmm.0.009175-0","article-title":"Distribution of the leptospiral immunoglobulin-like (Lig) genes in pathogenic <italic>Leptospira<\/italic> spp. and application of ligB to typing leptospiral isolates","volume":"58","author":"GM Cerqueira","year":"2009","journal-title":"J Med Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1128\/JCM.30.9.2219-2224.1992","article-title":"Polymerase chain reaction for detection of <italic>Leptospira<\/italic> spp. in clinical sample","volume":"30","author":"F M\u00e9rien","year":"1992","journal-title":"J Clin Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1099\/jmm.0.45860-0","article-title":"Detection of pathogenic leptospires by real-time quantitative PCR","volume":"54","author":"PN Levett","year":"2005","journal-title":"J Med Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"3954","DOI":"10.1128\/JCM.00336-06","article-title":"Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis","volume":"44","author":"L Salaun","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1016\/j.ymeth.2010.02.006","article-title":"ScreenClust: Advanced statistical software for supervised and unsupervised high resolution melting (HRM) analysis","volume":"50","author":"V Reja","year":"2010","journal-title":"Methods"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1469-0691.2007.01786.x","article-title":"Guidelines for the validation and application of typing methods for use in bacterial epidemiology","volume":"13","author":"A van Belkum","year":"2007","journal-title":"Clin Microbiol Inf"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/JCM.26.11.2465-2466.1988","article-title":"Numerical index of the discriminatory ability of typing systems: an application of Simpson&apos;s index of diversity","volume":"26","author":"PR Hunter","year":"1988","journal-title":"J Clin Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1469-0691.1996.tb00193.x","article-title":"Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems","volume":"2","author":"MJ Struelens","year":"1996","journal-title":"Clin Microbiol Infect"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1128\/JCM.02452-10","article-title":"Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences","volume":"49","author":"P Bourhy","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/0732-8893(95)00145-3","article-title":"Use of nondenaturing silver-stained polyacrylamide gel analysis of polymerase chain reaction amplification products for the differential diagnosis of Leptospira interrogans infection","volume":"22","author":"MA Oliveira","year":"1995","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0923-2508(00)00156-X","article-title":"Interest of partial 16S rDNA gene sequences to resolve heterogeneities between Leptospira collections: application to L. meyeri","volume":"151","author":"D Postic","year":"2000","journal-title":"Res Microbiol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"e112356","DOI":"10.1371\/journal.pone.0112356","article-title":"Sensitive real-time PCR detection of pathogenic Leptospira spp. and a comparison of nucleic acid amplification methods for the diagnosis of leptospirosis","volume":"9","author":"JJ Waggoner","year":"2014","journal-title":"PLoS One"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"e112312","DOI":"10.1371\/journal.pone.0112312","article-title":"Direct detection and differentiation of pathogenic Leptospira species using a multi-gene targeted real time PCR approach","volume":"9","author":"AS Ferreira","year":"2014","journal-title":"PLoS One"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"e1954","DOI":"10.1371\/journal.pntd.0001954","article-title":"A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species","volume":"7","author":"S Boonsilp","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"628","DOI":"10.4269\/ajtmh.2008.78.628","article-title":"Evaluation of a modified pulsed-field gel electrophoresis approach for the identification of Leptospira serovars","volume":"78","author":"RL Galloway","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1128\/JCM.05931-11","article-title":"Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features","volume":"50","author":"P Bourhy","year":"2012","journal-title":"J Clin Microbiol"},{"issue":"5","key":"ref53","doi-asserted-by":"crossref","first-page":"e20377","DOI":"10.1371\/journal.pone.0020377","article-title":"Seasonality of human leptospirosis in Reunion Island (Indian Ocean) ans its association with meteorological data","volume":"6","author":"A Desvars","year":"2011","journal-title":"PLoS One"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0127430","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T20:22:52Z","timestamp":1599250972000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0127430"}},"subtitle":[],"editor":[{"given":"Yung-Fu","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,7,8]]},"references-count":53,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2015,7,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0127430","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,8]]}}}