{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:16:07Z","timestamp":1789755367310,"version":"4.0.1"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T00:00:00Z","timestamp":1432598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0128082","type":"journal-article","created":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T18:03:22Z","timestamp":1432663402000},"page":"e0128082","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Culture Temperature Affects Human Chondrocyte Messenger RNA Expression in Monolayer and Pellet Culture Systems"],"prefix":"10.1371","volume":"10","author":[{"given":"Akira","family":"Ito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momoko","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Tajino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoki","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotaka","family":"Iijima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangkai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Aoyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kuroki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,5,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0142-9612(92)90001-5","article-title":"Cartilage and diarthrodial joints as paradigms for hierarchical materials and structures","volume":"13","author":"VC Mow","year":"1992","journal-title":"Biomaterials"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"460","DOI":"10.2106\/00004623-198264030-00022","article-title":"The response of articular cartilage to mechanical injury","volume":"64","author":"HJ Mankin","year":"1982","journal-title":"J Bone Joint Surg Am"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.2106\/00004623-199173090-00004","article-title":"The potential for regeneration of articular cartilage in defects created by chondral shaving and subchondral abrasion. An experimental investigation in rabbits","volume":"73","author":"HK Kim","year":"1991","journal-title":"J Bone Joint Surg Am"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.joca.2013.04.008","article-title":"Cell-based approaches to joint surface repair: a research perspective","volume":"21","author":"AJ Roelofs","year":"2013","journal-title":"Osteoarthritis Cartilage"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1056\/NEJM199410063311401","article-title":"Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation","volume":"331","author":"M Brittberg","year":"1994","journal-title":"N Engl J Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/267531a0","article-title":"Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture","volume":"267","author":"K von der Mark","year":"1977","journal-title":"Nature"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1053\/joca.2001.0482","article-title":"Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture","volume":"10","author":"M Schnabel","year":"2002","journal-title":"Osteoarthritis Cartilage"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1002\/jcp.10481","article-title":"Control of human articular chondrocyte differentiation by reduced oxygen tension","volume":"199","author":"CL Murphy","year":"2004","journal-title":"J Cell Physiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.bbrc.2013.05.052","article-title":"Chondrogenic capacity and alterations in hyaluronan synthesis of cultured human osteoarthritic chondrocytes","volume":"435","author":"Y Ono","year":"2013","journal-title":"Biochem Biophys Res Commun"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.knee.2009.02.004","article-title":"Immunohistochemical study of collagen types I and II and procollagen IIA in human cartilage repair tissue following autologous chondrocyte implantation","volume":"16","author":"S Roberts","year":"2009","journal-title":"Knee"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1097\/00003086-200005000-00020","article-title":"Two- to 9-year outcome after autologous chondrocyte transplantation of the knee","author":"L Peterson","year":"2000","journal-title":"Clin Orthop Relat Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.joca.2005.04.020","article-title":"Variations in matrix composition and GAG fine structure among scaffolds for cartilage tissue engineering","volume":"13","author":"JK Mouw","year":"2005","journal-title":"Osteoarthritis Cartilage"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1089\/ten.2006.0246","article-title":"Matrix-induced autologous chondrocyte implantation (MACI): biological and histological assessment","volume":"13","author":"MH Zheng","year":"2007","journal-title":"Tissue Eng"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"169","DOI":"10.4081\/1208","article-title":"Adult mesenchymal stem cells for bone and cartilage engineering: effect of scaffold materials","volume":"52","author":"A Gigante","year":"2008","journal-title":"Eur J Histochem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/S0140-6736(10)60668-X","article-title":"Regeneration of the articular surface of the rabbit synovial joint by cell homing: a proof of concept study","volume":"376","author":"CH Lee","year":"2010","journal-title":"Lancet"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.1007\/s11999-011-1857-3","article-title":"The role of growth factors in cartilage repair","volume":"469","author":"LA Fortier","year":"2011","journal-title":"Clin Orthop Relat Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.injury.2011.05.035","article-title":"Chondrocytes or adult stem cells for cartilage repair: the indisputable role of growth factors","volume":"43","author":"AM Freyria","year":"2012","journal-title":"Injury"},{"key":"ref18","first-page":"560","article-title":"Biosynthetic response of passaged chondrocytes in a type II collagen scaffold to mechanical compression","volume":"64","author":"CR Lee","year":"2003","journal-title":"J Biomed Mater Res A"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1002\/jor.20086","article-title":"Chondrocyte response to cyclic hydrostatic pressure in alginate versus pellet culture","volume":"24","author":"SH Elder","year":"2006","journal-title":"J Orthop Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/j.joca.2012.04.019","article-title":"Dynamic compression improves biosynthesis of human zonal chondrocytes from osteoarthritis patients","volume":"20","author":"JE Jeon","year":"2012","journal-title":"Osteoarthritis Cartilage"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s00441-014-1970-1","article-title":"Hypertonic conditions enhance cartilage formation in scaffold-free primary chondrocyte cultures","volume":"358","author":"JH Ylarinne","year":"2014","journal-title":"Cell Tissue Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1053\/joca.2001.0477","article-title":"Redifferentiation of dedifferentiated bovine articular chondrocytes in alginate culture under low oxygen tension","volume":"10","author":"C Domm","year":"2002","journal-title":"Osteoarthritis Cartilage"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/j.joca.2010.07.004","article-title":"Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytes","volume":"18","author":"CT Buckley","year":"2010","journal-title":"Osteoarthritis Cartilage"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.joca.2013.01.007","article-title":"Hypoxia-induced collagen crosslinking as a mechanism for enhancing mechanical properties of engineered articular cartilage","volume":"21","author":"EA Makris","year":"2013","journal-title":"Osteoarthritis Cartilage"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"234","DOI":"10.3109\/17453674.2011.566135","article-title":"Combined 3D and hypoxic culture improves cartilage-specific gene expression in human chondrocytes","volume":"82","author":"CB Foldager","year":"2011","journal-title":"Acta Orthop"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1007\/s10776-002-0643-2","article-title":"Effect of heat stimulation on viability and proteoglycan metabolism of cultured chondrocytes: preliminary report","volume":"8","author":"T Hojo","year":"2003","journal-title":"J Orthop Sci"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.arthro.2010.10.019","article-title":"The effect of irrigation solution at different temperatures on articular cartilage metabolism","volume":"27","author":"B Kocaoglu","year":"2011","journal-title":"Arthroscopy"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e91561","DOI":"10.1371\/journal.pone.0091561","article-title":"Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culture","volume":"9","author":"J Chen","year":"2014","journal-title":"PLoS One"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S0049-0172(05)80002-2","article-title":"Treating arthritis with locally applied heat or cold","volume":"24","author":"FG Oosterveld","year":"1994","journal-title":"Semin Arthritis Rheum"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.arthro.2005.01.011","article-title":"Effect of arthroscopy and continuous cryotherapy on the intra-articular temperature of the knee","volume":"21","author":"G Sanchez-Inchausti","year":"2005","journal-title":"Arthroscopy"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"96","DOI":"10.3109\/02656736.2014.880857","article-title":"Optimum temperature for extracellular matrix production by articular chondrocytes","volume":"30","author":"A Ito","year":"2014","journal-title":"Int J Hyperthermia"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1298\/jjpta.Vol17_003","article-title":"Effects of the thermal environment on articular chondrocyte metabolism: a fundamental study to facilitate establishment of an effective thermotherapy for osteoarthritis","volume":"17","author":"A Ito","year":"2014","journal-title":"J Jpn Phys Ther Assoc"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1016\/j.ultrasmedbio.2012.06.005","article-title":"Low-intensity pulsed ultrasound inhibits messenger RNA expression of matrix metalloproteinase-13 induced by interleukin-1beta in chondrocytes in an intensity-dependent manner","volume":"38","author":"A Ito","year":"2012","journal-title":"Ultrasound Med Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1148\/123.2.463","article-title":"Cellular responses to combinations of hyperthermia and radiation","volume":"123","author":"WC Dewey","year":"1977","journal-title":"Radiology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3109\/02656738909140444","article-title":"Heat-induced damage to HeLa-S3 cells: correlation of viability, permeability, osmosensitivity, phase-contrast light-, scanning electron- and transmission electron-microscopical findings","volume":"5","author":"DN Wheatley","year":"1989","journal-title":"Int J Hyperthermia"},{"key":"ref36","first-page":"Appendix 4K","article-title":"Quantitation of nucleic acids with absorption spectroscopy","volume":"Appendix 4","author":"S Gallagher","year":"2001","journal-title":"Curr Protoc Protein Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"210","DOI":"10.3109\/02656736.2014.906048","article-title":"Evaluation of reference genes for human chondrocytes cultured in several different thermal environments","volume":"30","author":"A Ito","year":"2014","journal-title":"Int J Hyperthermia"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1002\/(SICI)1097-0290(19970720)55:2<328::AID-BIT10>3.0.CO;2-D","article-title":"Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells","volume":"55","author":"S Chuppa","year":"1997","journal-title":"Biotechnol Bioeng"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1023\/A:1007919921991","article-title":"Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues","volume":"23","author":"A Moore","year":"1997","journal-title":"Cytotechnology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1023\/A:1014088919546","article-title":"Increased productivity of recombinant tissular plasminogen activator (t-PA) by butyrate and shift of temperature: a cell cycle phases analysis","volume":"36","author":"V Hendrick","year":"2001","journal-title":"Cytotechnology"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/bit.10566","article-title":"Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells","volume":"82","author":"SK Yoon","year":"2003","journal-title":"Biotechnol Bioeng"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1016\/j.biomaterials.2009.11.024","article-title":"Extraction of high quality RNA from polysaccharide matrices using cetyltrimethylammonium bromide","volume":"31","author":"L Wang","year":"2010","journal-title":"Biomaterials"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0167-4838(99)00119-3","article-title":"New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase","volume":"1432","author":"MA Sirover","year":"1999","journal-title":"Biochim Biophys Acta"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1152\/japplphysiol.00821.2002","article-title":"Citrate synthase expression and enzyme activity after endurance training in cardiac and skeletal muscles","volume":"94","author":"PM Siu","year":"2003","journal-title":"J Appl Physiol (1985)"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1002\/bit.25241","article-title":"The effect of oxygen tension on human articular chondrocyte matrix synthesis: integration of experimental and computational approaches","volume":"111","author":"S Li","year":"2014","journal-title":"Biotechnol Bioeng"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2613.2008.00625.x","article-title":"Involvement of the cytoskeletal elements in articular cartilage homeostasis and pathology","volume":"90","author":"EJ Blain","year":"2009","journal-title":"Int J Exp Pathol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1083\/jcb.106.1.161","article-title":"Microfilament modification by dihydrocytochalasin B causes retinoic acid-modulated chondrocytes to reexpress the differentiated collagen phenotype without a change in shape","volume":"106","author":"PD Benya","year":"1988","journal-title":"J Cell Biol"},{"key":"ref48","first-page":"364","article-title":"Proteoglycan and collagen synthesis are correlated with actin organization in dedifferentiating chondrocytes","volume":"56","author":"F Mallein-Gerin","year":"1991","journal-title":"Eur J Cell Biol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1023\/A:1004095207330","article-title":"Confocal analysis of cytoskeletal organisation within isolated chondrocyte sub-populations cultured in agarose","volume":"32","author":"BD Idowu","year":"2000","journal-title":"Histochem J"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1002\/jor.1100130404","article-title":"Influence of short-term hydrostatic pressure on organization of stress fibers in cultured chondrocytes","volume":"13","author":"JJ Parkkinen","year":"1995","journal-title":"J Orthop Res"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/bit.20717","article-title":"Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin","volume":"93","author":"JY Baik","year":"2006","journal-title":"Biotechnol Bioeng"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1038\/8792","article-title":"Sox9 is required for cartilage formation","volume":"22","author":"W Bi","year":"1999","journal-title":"Nat Genet"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1101\/gad.1017802","article-title":"The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6","volume":"16","author":"H Akiyama","year":"2002","journal-title":"Genes Dev"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/ng0697-174","article-title":"SOX9 directly regulates the type-II collagen gene","volume":"16","author":"DM Bell","year":"1997","journal-title":"Nat Genet"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"10738","DOI":"10.1074\/jbc.275.15.10738","article-title":"SOX9 enhances aggrecan gene promoter\/enhancer activity and is up-regulated by retinoic acid in a cartilage-derived cell line, TC6","volume":"275","author":"I Sekiya","year":"2000","journal-title":"J Biol Chem"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"4778","DOI":"10.1074\/jbc.M707729200","article-title":"Hypoxia promotes the differentiated human articular chondrocyte phenotype through SOX9-dependent and-independent pathways","volume":"283","author":"JE Lafont","year":"2008","journal-title":"J Biol Chem"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1002\/btpr.186","article-title":"Pellet culture elicits superior chondrogenic redifferentiation than alginate-based systems","volume":"25","author":"P Bernstein","year":"2009","journal-title":"Biotechnol Prog"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"R133","DOI":"10.1186\/ar2800","article-title":"Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation","volume":"11","author":"T Dehne","year":"2009","journal-title":"Arthritis Res Ther"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1016\/j.joca.2012.06.016","article-title":"Redifferentiation of dedifferentiated human articular chondrocytes: comparison of 2D and 3D cultures","volume":"20","author":"MM Caron","year":"2012","journal-title":"Osteoarthritis Cartilage"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"5600","DOI":"10.1016\/j.biomaterials.2011.04.033","article-title":"Effects of biomimetic surfaces and oxygen tension on redifferentiation of passaged human fibrochondrocytes in 2D and 3D cultures","volume":"32","author":"GK Tan","year":"2011","journal-title":"Biomaterials"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/s00441-011-1193-7","article-title":"Effects of oxygen and culture system on in vitro propagation and redifferentiation of osteoarthritic human articular chondrocytes","volume":"347","author":"K Schrobback","year":"2012","journal-title":"Cell Tissue Res"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"e58865","DOI":"10.1371\/journal.pone.0058865","article-title":"The interplay between chondrocyte redifferentiation pellet size and oxygen concentration","volume":"8","author":"BK Babur","year":"2013","journal-title":"PLoS One"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0128082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T08:10:22Z","timestamp":1540282222000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0128082"}},"subtitle":[],"editor":[{"given":"Andre","family":"van Wijnen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,26]]},"references-count":62,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0128082","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,26]]}}}