{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:01:09Z","timestamp":1775606469906,"version":"3.50.1"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T00:00:00Z","timestamp":1432252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0128120","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T17:55:31Z","timestamp":1432317331000},"page":"e0128120","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["Extended-Spectrum \u03b2-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolated from Egyptian Patients with Suspected Blood Stream Infection"],"prefix":"10.1371","volume":"10","author":[{"given":"H. M.","family":"Abdallah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. B.","family":"Wintermans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. A.","family":"Reuland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Koek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"al Naiemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Ammar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. A.","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M. J. E.","family":"Vandenbroucke-Grauls","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,5,22]]},"reference":[{"key":"ref1","author":"SC Piscitelli","year":"2011"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/2047-2994-2-1","article-title":"Access to antibiotics: a safety and equity challenge for the next decade","volume":"2","author":"J Carlet","year":"2013","journal-title":"Antimicrob Resist Infect Control"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.mib.2005.08.016","article-title":"Beta-lactam antibiotic resistance: a current structural perspective","volume":"8","author":"MS Wilke","year":"2005","journal-title":"Curr Opin Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.tim.2006.07.008","article-title":"The beta-lactamase threat in Enterobacteriaceae, Pseudomonas and Acinetobacter","volume":"14","author":"DM Livermore","year":"2006","journal-title":"Trends Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1002\/med.20210","article-title":"New definitions of extended-spectrum \u03b2-lactamase conferring worldwide emerging antibiotic resistance","volume":"32","author":"JH Lee","year":"2012","journal-title":"Med Res Rev"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.18.4.657-686.2005","article-title":"Extended-spectrum beta-lactamases: a clinical update","volume":"18","author":"DL Paterson","year":"2005","journal-title":"Clin Microbiol Rev"},{"key":"ref7","article-title":"Crit Rev Microbiol","author":"LM Biehl","year":"2014"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.vetmic.2014.01.017","article-title":"Extended-spectrum \u03b2-lactamase, carbapenemase and AmpC producing Enterobacteriaceae in companion animals","volume":"170","author":"JE Rubin","year":"2014","journal-title":"Vet Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1128\/AAC.00155-06","article-title":"Antibiotic coresistance in extended-spectrum-beta-lactamase-producing Enterobacteriaceae and in vitro activity of tigecycline","volume":"50","author":"I Morosini M-","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1128\/CMR.05035-11","article-title":"Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions","volume":"25","author":"LS Tzouvelekis","year":"2012","journal-title":"Clin Microbiol Rev"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.ijantimicag.2004.01.010","article-title":"Determining incidence of extended spectrum beta-lactamase producing Enterobacteriaceae, vancomycin-resistant Enterococcus faecium and methicillin-resistant Staphylococcus aureus in 38 centres from 17 countries: the PEARLS study 2001\u20132002","volume":"24","author":"SK Bouchillon","year":"2004","journal-title":"Int J Antimicrob Agents"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e61","DOI":"10.1016\/j.ajic.2011.04.009","article-title":"Antimicrobial resistance in pathogens causing nosocomial bloodstream infections in university hospitals in Egypt","volume":"39","author":"T Saied","year":"2011","journal-title":"Am J Infect Control"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1089\/mdr.2010.0063","article-title":"CTX-M-15-producing Escherichia coli clinical isolates in Cairo (Egypt), including isolates of clonal complex ST10 and clones ST131, ST73, and ST405 in both community and hospital settings","volume":"17","author":"N Fam","year":"2011","journal-title":"Microb Drug Resist"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.jgar.2013.06.003","article-title":"NDM-1- and OXA-163-producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012","volume":"1","author":"MO Abdelaziz","year":"2013","journal-title":"J Glob Antimicrob Resist"},{"key":"ref15","first-page":"342","author":"BS Nagoba","year":"2007"},{"key":"ref16","unstructured":"EUCAST. European Committee on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters European Committee on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters. 2014; 0\u201379."},{"key":"ref17","unstructured":"Al Naiemi N, Cohen Stuart J, Leverstein van Hall M. NVMM Guideline Laboratory detection of highly resistant microorganisms (HRMO), version 2.0. 2012; Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.nvmm.nl\/richtlijnen\/hrmo-laboratory-detection-highly-resistant-microorganisms\" xlink:type=\"simple\">http:\/\/www.nvmm.nl\/richtlijnen\/hrmo-laboratory-detection-highly-resistant-microorganisms<\/ext-link>"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1128\/JCM.00130-09","article-title":"Sensitive screening tests for suspected class A carbapenemase production in species of Enterobacteriaceae","volume":"47","author":"F Pasteran","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/JCM.01922-08","article-title":"Evaluation of boronic acid disk tests for differentiating KPC-possessing Klebsiella pneumoniae isolates in the clinical laboratory","volume":"47","author":"A Tsakris","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1089\/mdr.2004.10.334","article-title":"Prevalence of beta-lactamases among ampicillin-resistant Escherichia coli and Salmonella isolated from food animals in Denmark","volume":"10","author":"I Olesen","year":"2004","journal-title":"Microb Drug Resist"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1128\/JCM.42.6.2432-2437.2004","article-title":"SHV-12-like extended-spectrum-beta-lactamase-producing strains of Salmonella enterica serotypes Babelsberg and Enteritidis isolated in France among infants adopted from Mali","volume":"42","author":"F-X Weill","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1128\/JCM.41.1.460-462.2003","article-title":"Characterization of the first extended-spectrum beta-lactamase-producing Salmonella isolate identified in Canada","volume":"41","author":"MR Mulvey","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","article-title":"Multiplex PCR for detection of acquired carbapenemase genes","volume":"70","author":"L Poirel","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"3456","DOI":"10.1128\/AEM.69.6.3456-3461.2003","article-title":"Evaluation of DNA Extraction Methods for Use in Combination with SYBR Green I Real-Time PCR To Detect Salmonella enterica Serotype Enteritidis in Poultry","volume":"69","author":"D De Medici","year":"2003","journal-title":"Appl Environ Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1128\/AAC.00611-06","article-title":"Identification of CTX-M-type extended-spectrum-beta-lactamase genes using real-time PCR and pyrosequencing","volume":"51","author":"T Naas","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1476-0711-11-9","article-title":"A rapid low-cost real-time PCR for the detection of Klebsiella pneumonia carbapenemase genes","volume":"11","author":"L Wang","year":"2012","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1128\/AEM.66.10.4555-4558.2000","article-title":"Rapid and simple determination of the Escherichia coli phylogenetic group","volume":"66","author":"O Clermont","year":"2000","journal-title":"Appl Environ Microbiol"},{"key":"ref28","first-page":"537","article-title":"Small Animal Critical Care Medicine","author":"S Epstein","year":"2015"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/1756-0500-2-76","article-title":"Nosocomial blood stream infection in intensive care units at Assiut University Hospitals (Upper Egypt) with special reference to extended spectrum beta-lactamase producing organisms","volume":"2","author":"SH Ahmed","year":"2009","journal-title":"BMC Res Notes"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1093\/jac\/dkl483","article-title":"CTX-M: changing the face of ESBLs in Europe","volume":"59","author":"DM Livermore","year":"2007","journal-title":"J Antimicrob Chemother"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"110","DOI":"10.3389\/fmicb.2012.00110","article-title":"CTX-M Enzymes: Origin and Diffusion","volume":"3","author":"R Cant\u00f3n","year":"2012","journal-title":"Front Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2781","DOI":"10.1093\/jac\/dkr384","article-title":"Emergence of NDM-1-producing Klebsiella pneumoniae in Morocco","volume":"66","author":"L Poirel","year":"2011","journal-title":"J Antimicrob Chemother"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1099\/jmm.0.059014-0","article-title":"Emergence and spread of NDM-1 producer Enterobacteriaceae with contribution of IncX3 plasmids in the United Arab Emirates","volume":"62","author":"A Sonnevend","year":"2013","journal-title":"J Med Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1089\/mdr.2012.0078","article-title":"First report of New Delhi metallo-beta-lactamase-1-producing Klebsiella pneumoniae in Iran","volume":"19","author":"F Shahcheraghi","year":"2013","journal-title":"Microb Drug Resist"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"275","DOI":"10.3109\/00365540903493715","article-title":"Extended-spectrum beta-lactamase-producing Escherichia coli in patients with travellers\u2019 diarrhoea","volume":"42","author":"J Tham","year":"2010","journal-title":"Scand J Infect Dis"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1111\/j.1469-0691.2012.03947.x","article-title":"High prevalence of ESBL-producing Enterobacteriaceae carriage in Dutch community patients with gastrointestinal complaints","volume":"19","author":"EA Reuland","year":"2013","journal-title":"Clin Microbiol Infect"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1093\/jac\/47.6.755","article-title":"Outer membrane protein change combined with co-existing TEM-1 and SHV-1 beta-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniae","volume":"47","author":"T-L Wu","year":"2001","journal-title":"J Antimicrob Chemother"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1128\/CMR.14.4.933-951.2001","article-title":"Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat","volume":"14","author":"PA Bradford","year":"2001","journal-title":"Clin Microbiol Rev"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1476-0711-12-16","article-title":"Detection of new SHV-12, SHV-5 and SHV-2a variants of extended spectrum beta-lactamase in Klebsiella pneumoniae in Egypt","volume":"12","author":"EA Newire","year":"2013","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"4667","DOI":"10.1128\/AAC.49.11.4667-4670.2005","article-title":"Virulence factors of Escherichia coli isolates that produce CTX-M-type extended-spectrum beta-lactamases","volume":"49","author":"JDD Pitout","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"ref41","first-page":"361","article-title":"Phylogenetic Groups and Virulence Factors in Pathogenic and Commensal Strains of Escherichia coli and Their Association with blaCTX-M","volume":"40","author":"S Lee","year":"2010","journal-title":"Ann Clin Lab Sci"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"54","DOI":"10.3201\/eid1101.040257","article-title":"Genetic background of Escherichia coli and extended-spectrum beta-lactamase type","volume":"11","author":"C Branger","year":"2005","journal-title":"Emerg Infect Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0128120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T22:24:20Z","timestamp":1540247060000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0128120"}},"subtitle":[],"editor":[{"given":"Massimiliano","family":"Galdiero","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,22]]},"references-count":42,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0128120","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,22]]}}}