{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:27:14Z","timestamp":1779244034274,"version":"3.51.4"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2015,6,9]],"date-time":"2015-06-09T00:00:00Z","timestamp":1433808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0128185","type":"journal-article","created":{"date-parts":[[2015,6,9]],"date-time":"2015-06-09T17:58:54Z","timestamp":1433872734000},"page":"e0128185","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["A Mutant Library Approach to Identify Improved Meningococcal Factor H Binding Protein Vaccine Antigens"],"prefix":"10.1371","volume":"10","author":[{"given":"Monica","family":"Konar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaella","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Walter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolando","family":"Pajon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter T.","family":"Beernink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,6,9]]},"reference":[{"issue":"Suppl 8","key":"ref1","doi-asserted-by":"crossref","first-page":"I113","DOI":"10.1016\/j.vaccine.2008.11.058","article-title":"Microbial complement inhibitors as vaccines","volume":"26","author":"S Meri","year":"2008","journal-title":"Vaccine"},{"issue":"5","key":"ref2","first-page":"421","article-title":"Novel directions in HIV-1 vaccines revealed from clinical trials. Current opinion","volume":"8","author":"JL Excler","year":"2013","journal-title":"HIV and AIDS"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.vaccine.2006.08.001","article-title":"Phase 1 first-in-human studies of the reactogenicity and immunogenicity of a recombinant meningococcal NspA vaccine in healthy adults","volume":"25","author":"SA Halperin","year":"2007","journal-title":"Vaccine"},{"issue":"6686","key":"ref4","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1038\/31514","article-title":"The antigenic structure of the HIV gp120 envelope glycoprotein","volume":"393","author":"R Wyatt","year":"1998","journal-title":"Nature"},{"issue":"27","key":"ref5","doi-asserted-by":"crossref","first-page":"24825","DOI":"10.1074\/jbc.M302803200","article-title":"Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential","volume":"278","author":"L Vandeputte-Rutten","year":"2003","journal-title":"J Biol Chem"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1086\/649209","article-title":"Changes in <italic>Neisseria meningitidis<\/italic> disease epidemiology in the United States, 1998\u20132007: implications for prevention of meningococcal disease","volume":"50","author":"AC Cohn","year":"2010","journal-title":"Clin Infect Dis"},{"issue":"10","key":"ref7","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1097\/INF.0b013e31826327e4","article-title":"Safety and immunogenicity of a meningococcal B bivalent rLP2086 vaccine in healthy toddlers aged 18\u201336 months: a phase 1 randomized-controlled clinical trial","volume":"31","author":"HS Marshall","year":"2012","journal-title":"Pediatr Infect Dis J"},{"issue":"10","key":"ref8","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1086\/656741","article-title":"Multicenter, open-label, randomized phase II controlled trial of an investigational recombinant meningococcal serogroup B vaccine with and without outer membrane vesicles, administered in infancy","volume":"51","author":"J Findlow","year":"2010","journal-title":"Clin Infect Dis"},{"issue":"5459","key":"ref9","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1126\/science.287.5459.1809","article-title":"Complete genome sequence of <italic>Neisseria meningitidis<\/italic> serogroup B strain MC58","volume":"287","author":"H Tettelin","year":"2000","journal-title":"Science"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"501","DOI":"10.4049\/jimmunol.177.1.501","article-title":"The meningococcal vaccine candidate GNA1870 binds the complement regulatory protein factor H and enhances serum resistance","volume":"177","author":"G Madico","year":"2006","journal-title":"J Immunol"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1128\/IAI.01191-08","article-title":"Binding of complement factor H (fH) to <italic>Neisseria meningitidis<\/italic> is specific for human fH and inhibits complement activation by rat and rabbit sera","volume":"77","author":"DM Granoff","year":"2009","journal-title":"Infect Immun"},{"issue":"11","key":"ref12","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1128\/CVI.00517-14","article-title":"Heterogeneity in rhesus macaque complement Factor H binding to meningococcal Factor H binding protein (FHbp) informs selection of primates to assess immunogenicity of FHbp-based vaccines","volume":"21","author":"PT Beernink","year":"2014","journal-title":"Clin Vaccine Immunol"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1084\/jem.20021911","article-title":"Vaccination against <italic>Neisseria meningitidis<\/italic> using three variants of the lipoprotein GNA1870","volume":"197","author":"V Masignani","year":"2003","journal-title":"J Exp Med"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1128\/IAI.72.4.2088-2100.2004","article-title":"Vaccine potential of the <italic>Neisseria meningitidis<\/italic> 2086 lipoprotein","volume":"72","author":"LD Fletcher","year":"2004","journal-title":"Infect Immun"},{"issue":"37","key":"ref15","doi-asserted-by":"crossref","first-page":"6086","DOI":"10.1016\/j.vaccine.2010.06.083","article-title":"Broad vaccine coverage predicted for a bivalent recombinant factor H binding protein based vaccine to prevent serogroup B meningococcal disease","volume":"28","author":"HQ Jiang","year":"2010","journal-title":"Vaccine"},{"issue":"29\u201330","key":"ref16","doi-asserted-by":"crossref","first-page":"4728","DOI":"10.1016\/j.vaccine.2011.04.095","article-title":"Immunogenicity of a meningococcal native outer membrane vesicle vaccine with attenuated endotoxin and over-expressed factor H binding protein in infant rhesus monkeys","volume":"29","author":"O Koeberling","year":"2011","journal-title":"Vaccine"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Granoff DM, Costa I, Konar M, Giuntini S, Van Rompay KK, Beernink PT. Binding of complement Factor H decreases protective anti-FHbp antibody responses of infant rhesus macaques immunized with a meningococcal serogroup B vaccine. J Infect Dis 101093\/infdis\/jiv081. 2015. <comment>doi: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1093\/infdis\/jiv081\" xlink:type=\"simple\">10.1093\/infdis\/jiv081<\/ext-link><\/comment> <object-id pub-id-type=\"pmid\">25676468<\/object-id>.","DOI":"10.1093\/infdis\/jiv081"},{"issue":"8","key":"ref18","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S1473-3099(12)70087-7","article-title":"Safety, immunogenicity, and tolerability of meningococcal serogroup B bivalent recombinant lipoprotein 2086 vaccine in healthy adolescents: a randomised, single-blind, placebo-controlled, phase 2 trial","volume":"12","author":"PC Richmond","year":"2012","journal-title":"The Lancet infectious diseases"},{"issue":"43","key":"ref19","doi-asserted-by":"crossref","first-page":"6163","DOI":"10.1016\/j.vaccine.2012.07.065","article-title":"A bivalent <italic>Neisseria meningitidis<\/italic> recombinant lipidated factor H binding protein vaccine in young adults: Results of a randomised, controlled, dose-escalation phase 1 trial","volume":"30","author":"PC Richmond","year":"2012","journal-title":"Vaccine"},{"issue":"11","key":"ref20","doi-asserted-by":"crossref","first-page":"e71","DOI":"10.1097\/INF.0b013e3181f59f6d","article-title":"Immunogenicity of two investigational serogroup B meningococcal vaccines in the first year of life: a randomized comparative trial","volume":"29","author":"MD Snape","year":"2010","journal-title":"Pediatr Infect Dis J"},{"issue":"5","key":"ref21","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1016\/j.vaccine.2010.11.064","article-title":"Influence of sequence variability on bactericidal activity sera induced by Factor H binding protein variant 1.1","volume":"29","author":"B Brunelli","year":"2011","journal-title":"Vaccine"},{"issue":"6","key":"ref22","doi-asserted-by":"crossref","first-page":"3606","DOI":"10.4049\/jimmunol.1003470","article-title":"A meningococcal factor H binding protein mutant that eliminates factor H binding enhances protective antibody responses to vaccination","volume":"186","author":"PT Beernink","year":"2011","journal-title":"J Immunol"},{"issue":"7240","key":"ref23","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1038\/nature07769","article-title":"<italic>Neisseria meningitidis<\/italic> recruits factor H using protein mimicry of host carbohydrates","volume":"458","author":"MC Schneider","year":"2009","journal-title":"Nature"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"e1002688","DOI":"10.1371\/journal.ppat.1002688","article-title":"The effect of human factor H on immunogenicity of meningococcal native outer membrane vesicle vaccines with over-expressed factor H binding protein","volume":"8","author":"PT Beernink","year":"2012","journal-title":"PLoS Pathog"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1128\/IAI.00103-12","article-title":"Design of meningococcal factor H binding protein mutant vaccines that do not bind human complement factor H","volume":"80","author":"R Pajon","year":"2012","journal-title":"Infect Immun"},{"issue":"46","key":"ref26","doi-asserted-by":"crossref","first-page":"5451","DOI":"10.1016\/j.vaccine.2013.08.099","article-title":"Meningococcal factor H-binding protein vaccines with decreased binding to human complement factor H have enhanced immunogenicity in human factor H transgenic mice","volume":"31","author":"R Rossi","year":"2013","journal-title":"Vaccine"},{"issue":"10","key":"ref27","doi-asserted-by":"crossref","first-page":"e1002981","DOI":"10.1371\/journal.ppat.1002981","article-title":"Design and evaluation of meningococcal vaccines through structure-based modification of host and pathogen molecules","volume":"8","author":"S Johnson","year":"2012","journal-title":"PLoS Pathog"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1128\/IAI.01183-13","article-title":"Nonfunctional variant 3 factor H binding proteins as meningococcal vaccine candidates","volume":"82","author":"S van der Veen","year":"2014","journal-title":"Infect Immun"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/srep00341","article-title":"A broadly cross-reactive monoclonal antibody against an epitope on the N-terminus of meningococcal fHbp","volume":"2","author":"DM Vu","year":"2012","journal-title":"Scientific reports"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1128\/IAI.73.2.1151-1160.2005","article-title":"The region comprising amino acids 100 to 255 of <italic>Neisseria meningitidis<\/italic> lipoprotein GNA 1870 elicits bactericidal antibodies","volume":"73","author":"MM Giuliani","year":"2005","journal-title":"Infect Immun"},{"issue":"9","key":"ref31","doi-asserted-by":"crossref","first-page":"3751","DOI":"10.1128\/IAI.05182-11","article-title":"Complement-mediated bactericidal activity of anti-factor H binding protein monoclonal antibodies against the meningococcus relies upon blocking factor H binding","volume":"79","author":"S Giuntini","year":"2011","journal-title":"Infect Immun"},{"issue":"4","key":"ref32","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1093\/infdis\/jit239","article-title":"Importance of inhibition of binding of complement factor H for serum bactericidal antibody responses to meningococcal factor H-binding protein vaccines","volume":"208","author":"M Konar","year":"2013","journal-title":"J Infect Dis"},{"issue":"6","key":"ref33","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1128\/IAI.00033-08","article-title":"Bactericidal antibody responses induced by meningococcal recombinant chimeric factor H-binding protein vaccines","volume":"76","author":"PT Beernink","year":"2008","journal-title":"Infect Immun"},{"issue":"9","key":"ref34","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1086\/597806","article-title":"Meningococcal factor H-binding protein variants expressed by epidemic capsular group A, W-135, and X strains from Africa","volume":"199","author":"PT Beernink","year":"2009","journal-title":"J Infect Dis"},{"issue":"2","key":"ref35","first-page":"139","article-title":"Meningococcal strains isolated from teenage patients during the serogroup B vaccination trial in Norway: serotyping, serosubtyping, immunotyping and clonal analysis","volume":"14","author":"LO Froholm","year":"1991","journal-title":"NIPH annals"},{"issue":"9","key":"ref36","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1128\/JB.01331-10","article-title":"Genome sequence of <italic>Neisseria meningitidis<\/italic> serogroup B strain H44\/76","volume":"193","author":"JR Piet","year":"2011","journal-title":"J Bacteriol"},{"issue":"9","key":"ref37","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1016\/j.vaccine.2009.12.027","article-title":"Frequency of factor H-binding protein modular groups and susceptibility to cross-reactive bactericidal activity in invasive meningococcal isolates","volume":"28","author":"R Pajon","year":"2010","journal-title":"Vaccine"},{"issue":"5","key":"ref38","doi-asserted-by":"crossref","first-page":"e01625","DOI":"10.1128\/mBio.01625-14","article-title":"Human factor H (FH) impairs protective meningococcal anti-FHbp antibody responses and the antibodies enhance FH binding","volume":"5","author":"I Costa","year":"2014","journal-title":"mBio"},{"issue":"5","key":"ref39","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1107\/S1744309111006154","article-title":"Structure of the uncomplexed <italic>Neisseria meningitidis<\/italic> factor H-binding protein fHbp (rLP2086)","volume":"67","author":"L Cendron","year":"2011","journal-title":"Acta Crystallogr Sect F Struct Biol Cryst Commun"},{"issue":"9","key":"ref40","doi-asserted-by":"crossref","first-page":"5606","DOI":"10.4049\/jimmunol.172.9.5606","article-title":"Protective activity of monoclonal antibodies to genome-derived neisserial antigen 1870, a <italic>Neisseria meningitidis<\/italic> candidate vaccine","volume":"172","author":"JA Welsch","year":"2004","journal-title":"J Immunol"},{"issue":"9","key":"ref41","doi-asserted-by":"crossref","first-page":"4232","DOI":"10.1128\/IAI.00367-08","article-title":"Fine antigenic specificity and cooperative bactericidal activity of monoclonal antibodies directed at the meningococcal vaccine candidate factor H-binding protein","volume":"76","author":"PT Beernink","year":"2008","journal-title":"Infect Immun"},{"issue":"8\u20139","key":"ref42","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1016\/j.molimm.2009.02.021","article-title":"A region of the N-terminal domain of meningococcal factor H-binding protein that elicits bactericidal antibody across antigenic variant groups","volume":"46","author":"PT Beernink","year":"2009","journal-title":"Mol Immunol"},{"issue":"1","key":"ref43","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.jmb.2008.12.005","article-title":"Epitope mapping of a bactericidal monoclonal antibody against the factor H binding protein of <italic>Neisseria meningitidis<\/italic>","volume":"386","author":"M Scarselli","year":"2009","journal-title":"J Mol Biol"},{"issue":"13","key":"ref44","doi-asserted-by":"crossref","first-page":"8738","DOI":"10.1074\/jbc.M808831200","article-title":"Structural basis for the immunogenic properties of the meningococcal vaccine candidate LP2086","volume":"284","author":"A Mascioni","year":"2009","journal-title":"J Biol Chem"},{"issue":"7","key":"ref45","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1128\/CVI.00103-10","article-title":"Impaired immunogenicity of a meningococcal factor H-binding protein vaccine engineered to eliminate factor H binding","volume":"17","author":"PT Beernink","year":"2010","journal-title":"Clin Vaccine Immunol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0128185","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T23:29:51Z","timestamp":1652311791000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0128185"}},"subtitle":[],"editor":[{"given":"Yung-Fu","family":"Chang","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,6,9]]},"references-count":45,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,6,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0128185","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,9]]}}}