{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:59:13Z","timestamp":1787021953976,"version":"build-2736575974"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,29]],"date-time":"2015-05-29T00:00:00Z","timestamp":1432857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0128694","type":"journal-article","created":{"date-parts":[[2015,5,29]],"date-time":"2015-05-29T14:33:19Z","timestamp":1432909999000},"page":"e0128694","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Mechanism of Testosterone Deficiency in the Transgenic Sickle Cell Mouse"],"prefix":"10.1371","volume":"10","author":[{"given":"Biljana","family":"Musicki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haolin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry R.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry R.","family":"Zirkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur L.","family":"Burnett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,5,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1097\/MOH.0b013e32835f55f9","article-title":"New insights into sickle cell disease: a disease of hypoxia","volume":"20","author":"K Sun","year":"2013","journal-title":"Curr Opin Hematol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1002\/ajh.21475","article-title":"Vasculopathy in sickle cell disease: Biology, pathophysiology, genetics, translational medicine, and new research directions","volume":"84","author":"GJ Kato","year":"2009","journal-title":"Am J Hematol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1111\/j.1440-1681.2007.04639.x","article-title":"Sickle cell disease: Role of reactive oxygen and nitrogen metabolites","volume":"34","author":"KC Wood","year":"2007","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1002\/jcp.22195","article-title":"Sickle cell anemia and vascular dysfunction: The nitric oxide connection","volume":"224","author":"I Akinsheye","year":"2010","journal-title":"J Cell Physiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev-med-120611-143127","article-title":"Sickle cell disease, vasculopathy, and therapeutics","volume":"64","author":"AA Kassim","year":"2013","journal-title":"Annu Rev Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"601","DOI":"10.7326\/0003-4819-85-5-601","article-title":"Oberleas D: Gonadal function abnormalities in Sickle cell anaemia. Studies in adult male patients","volume":"85","author":"AA Abbasi","year":"1976","journal-title":"Ann Int Med"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1186\/1756-0500-4-298","article-title":"Serum testosterone levels of HbSS (sickle cell disease) male subjects in Lagos, Nigeria","volume":"4","author":"EK Abudu","year":"2011","journal-title":"BMC Res Notes"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0009-8981(80)90469-6","article-title":"Endocrine function and Haemoglobinopathies: Relation between the sickle cell gene and circulatory levels of testosterone, LH and FSH in adult males","volume":"105","author":"OO Dada","year":"1980","journal-title":"Clin Chim Acta"},{"issue":"Suppl 1","key":"ref9","first-page":"12","article-title":"The mechanism of low testosterone levels in homozygous sickle-cell disease","volume":"43","author":"O Parshad","year":"1994","journal-title":"West Indian Med J"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1136\/pgmj.63.736.95","article-title":"Testicular dysfunction in men with sickle cell disease","volume":"63","author":"DN Osegbe","year":"1987","journal-title":"Postgrad Med J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1097\/MPH.0b013e31806451ac","article-title":"Children with sickle cell disease: growth and gonadal function after hematopoietic stem cell transplantation","volume":"29","author":"C Brachet","year":"2007","journal-title":"J Pediatr Hematol Oncol"},{"key":"ref12","first-page":"20","article-title":"Testosterone deficiency and extreme retardation of puberty in homozygous sickle-cell disease","volume":"44","author":"A Singhal","year":"1995","journal-title":"West Indian Med J"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1111\/j.2047-2927.2013.00084.x","article-title":"Testosterone replacement therapy does not promote priapism in hypogonadal men with sickle cell disease: 12\u2010month safety report","volume":"1","author":"BF Morrison","year":"2013","journal-title":"Andrology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/S0015-0282(16)57500-1","article-title":"Effect of age on testicular function in adult males with sickle cell anemia","volume":"63","author":"O Modebe","year":"1995","journal-title":"Fertil Steril"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/tropej\/38.6.307","article-title":"Endocrine functions in sickle cell anaemia patients","volume":"38","author":"MA el-Hazmi","year":"1992","journal-title":"J Trop Pediatr"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1095\/biolreprod44.1.135","article-title":"Interactions of human growth hormone and prolactin on pituitary and Leydig cell function in adult transgenic mice expressing the human growth hormone gene","volume":"44","author":"V Chandrashekar","year":"1991","journal-title":"Biol Reprod"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1126\/science.278.5339.876","article-title":"Transgenic knockout mice with exclusively human sickle hemoglobin and sickle cell disease","volume":"278","author":"C P\u00e1szty","year":"1997","journal-title":"Science"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3088","DOI":"10.1182\/blood-2006-08-039438","article-title":"Hemolysis in sickle cell mice causes pulmonary hypertension due to global impairment in nitric oxide bioavailability","volume":"109","author":"LL Hsu","year":"2007","journal-title":"Blood"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1095\/biolreprod53.1.103","article-title":"Regulation of rat luteinizing hormone B gene expression in transgenic mice by steroids and a gonadotropin-releasing hormone antagonist","volume":"53","author":"PC Fallest","year":"1995","journal-title":"Biol Reprod"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1210\/en.2012-2226","article-title":"Drug ligand-induced activation of translocator protein (TSPO) stimulates steroid production by aged brown Norway rat Leydig cells","volume":"154","author":"JY Chung","year":"2013","journal-title":"Endocrinology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1002\/j.1939-4640.2001.tb02227.x","article-title":"Purification of rat leydig cells: increased yields after unit-gravity sedimentation of collagenase-dispersed interstitial cells","volume":"22","author":"A Salva","year":"2001","journal-title":"J Androl"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"4061","DOI":"10.1073\/pnas.052712499","article-title":"Akt-dependent phosphorylation of endothelial nitric oxide synthase mediated penile erection","volume":"99","author":"KJ Hurt","year":"2002","journal-title":"Proc Natl Acad Sci. USA"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1042\/CS20100384","article-title":"Novel Nox homologues in the vasculature: Focusing on Nox4 and Nox5","volume":"120","author":"AC Montezano","year":"2011","journal-title":"Clin Sci (Lond)"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1586\/eem.09.41","article-title":"Antioxidant enzyme deficiencies and vascular disease","volume":"5","author":"J Loscalzo","year":"2010","journal-title":"Expert Rev Endocrinol Metab"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chemphyslip.2008.09.004","article-title":"Lipid peroxidation of membrane phospholipids generates hydroxy-alkenals and oxidized phospholipids active in physiological and\/or pathological conditions","volume":"157","author":"A Catala","year":"2009","journal-title":"Chem Phys Lipids"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1111\/jsm.12391","article-title":"Sildenafil promotes eNOS activation and inhibits NADPH oxidase in the transgenic sickle cell mouse penis","volume":"11","author":"B Musicki","year":"2014","journal-title":"J Sex Med"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3389\/fphys.2015.00015","article-title":"Chronic intermittent hypoxia increases rat sternohyoid muscle NADPH oxidase expression with attendant modest oxidative stress","volume":"6","author":"R Williams","year":"2015","journal-title":"Front Physiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1111\/j.2047-2927.2014.00225.x","article-title":"New targets for increasing endogenous testosterone production: clinical implications and review of the literature","volume":"2","author":"B Le","year":"2014","journal-title":"Andrology"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.mce.2006.12.004","article-title":"Is there a mitochondrial signaling complex facilitating cholesterol import?","volume":"265\u2013266","author":"V Papadopoulos","year":"2007","journal-title":"Mol Cell Endocrinol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.bbalip.2009.03.001","article-title":"Cholesterol transport in steroid biosynthesis: role of protein-protein interactions and implications in disease states","volume":"1791","author":"MB Rone","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1126\/science.1248725","article-title":"Structure of the mitochondrial translocator protein in complex with a diagnostic ligand","volume":"343","author":"L Jaremko","year":"2014","journal-title":"Science"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1111\/tra.12177","article-title":"Binding domain-driven intracellular trafficking of sterols for synthesis of steroid hormone, bile acid and oxysterols","volume":"15","author":"A Midzak","year":"2014","journal-title":"Traffic"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s00204-013-1024-6","article-title":"Hypoxaemia affects male reproduction: a case study of how to differentiate between primary and secondary hypoxic testicular toxicity due to chemical exposure","volume":"87","author":"EM Bomhard","year":"2013","journal-title":"Arch Toxicol"},{"key":"ref34","first-page":"532","article-title":"Pathophysiological insights in sickle cell disease","volume":"134","author":"MH Odie`vre","year":"2011","journal-title":"Indian J Med Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.reprotox.2012.12.002","article-title":"Apocynin and raisanberine alleviate intermittent hypoxia induced abnormal StAR and 3\u03b2-HSD and low testosterone by suppressing endoplasmic reticulum stress and activated p66Shc in rat testes","volume":"36","author":"GL Zhang","year":"2013","journal-title":"Reprod Toxicol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1016\/j.freeradbiomed.2013.08.163","article-title":"Signaling properties of 4-hydroxyalkenals formed by lipid peroxidation in diabetes","volume":"65","author":"G Cohen","year":"2013","journal-title":"Free Radic Biol Med"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"240","DOI":"10.2164\/jandrol.05075","article-title":"Aging and the brown Norway rat leydig cell antioxidant defense system","volume":"27","author":"L Luo","year":"2006","journal-title":"J Androl"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"929285","DOI":"10.1155\/2012\/929285","article-title":"The hypoxic testicle: physiology and pathophysiology","volume":"2012","author":"JG Reyes","year":"2012","journal-title":"Oxid Med Cell Longev"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"271","DOI":"10.2174\/1871529X10909040271","article-title":"A systems biology consideration of the vasculopathy of sickle cell anemia: the need for multi-modality chemo-prophylaxsis","volume":"9","author":"RP Hebbel","year":"2009","journal-title":"Cardiovasc Hematol Disord Drug Targets"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1530\/jrf.0.0970115","article-title":"Control of testicular vasomotion by testosterone and tubular factors in rats","volume":"97","author":"O Collin","year":"1993","journal-title":"J Reprod Fertil"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0022-5347(01)63058-1","article-title":"Testicular infarction associated with sickle cell disease","volume":"160","author":"NM Holmes","year":"1998","journal-title":"J Urol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"551xi","DOI":"10.1016\/S0090-4295(03)00482-5","article-title":"Repeated testicular infarction in a patient with sickle cell disease: a possible mechanism for testicular failure","volume":"62","author":"M Li","year":"2003","journal-title":"Urology"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/S0022-5347(01)62803-9","article-title":"Segmental testicular infarction due to sickle cell disease","volume":"160","author":"ON Gofrit","year":"1998","journal-title":"J Urol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1210\/jc.2009-1796","article-title":"EMAS Group. Characteristics of secondary, primary, and compensated hypogonadism in aging men: evidence from the European Male Ageing Study","volume":"95","author":"A Tajar","year":"2010","journal-title":"J Clin Endocrinol Metab"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1210\/jc.2007-1972","article-title":"Hypothalamic-pituitary-testicular axis disruptions in older men are differentially linked to age and modifiable risk factors: the European Male Aging Study","volume":"93","author":"FC Wu","year":"2008","journal-title":"J Clin Endocrinol Metab"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"3221","DOI":"10.1210\/jcem.87.7.8678","article-title":"Prospective study of the spontaneous course of subclinical hypothyroidism: prognostic value of thyrotropin, thyroid reserve, and thyroid antibodies","volume":"87","author":"G Huber","year":"2002","journal-title":"J Clin Endocrinol Metab"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1182\/blood-2005-07-2839","article-title":"Pathology of Berkeley sickle cell mice: similarities and differences with human sickle cell disease","volume":"107","author":"EA Manci","year":"2006","journal-title":"Blood"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"H39","DOI":"10.1152\/ajpheart.00162.2008","article-title":"Arginine therapy of transgenic-knockout sickle mice improves microvascular function by reducing non-nitric oxide vasodilators, hemolysis, and oxidative stress","volume":"295","author":"DK Kaul","year":"2008","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1016\/S0140-6736(14)61944-9","article-title":"Acute compartment syndrome secondary to rhabdomyolysis in a sickle cell trait patient","volume":"384","author":"A Ridha","year":"2014","journal-title":"Lancet"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1001\/jama.2014.15063","article-title":"Association of sickle cell trait with chronic kidney disease and albuminuria in African Americans","volume":"312","author":"RP Naik","year":"2014","journal-title":"JAMA"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0128694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T04:12:00Z","timestamp":1717906320000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0128694"}},"subtitle":[],"editor":[{"given":"Jean-Marc A","family":"Lobaccaro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,29]]},"references-count":50,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0128694","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,29]]}}}