{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:38:58Z","timestamp":1792229938361,"version":"4.4.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2015,6,5]],"date-time":"2015-06-05T00:00:00Z","timestamp":1433462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0128996","type":"journal-article","created":{"date-parts":[[2015,6,5]],"date-time":"2015-06-05T15:02:35Z","timestamp":1433516555000},"page":"e0128996","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":36,"title":["Effects of High Fat Feeding and Diabetes on Regression of Atherosclerosis Induced by Low-Density Lipoprotein Receptor Gene Therapy in LDL Receptor-Deficient Mice"],"prefix":"10.1371","volume":"10","author":[{"given":"Florian","family":"Willecke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chujun","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Oka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunying","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelley","family":"Barnhart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin E.","family":"Bornfeldt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira J.","family":"Goldberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,6,5]]},"reference":[{"issue":"22","key":"ref1","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1056\/NEJMoa1110874","article-title":"Effect of two intensive statin regimens on progression of coronary disease","volume":"365","author":"SJ Nicholls","year":"2011","journal-title":"N Engl J Med"},{"issue":"9","key":"ref2","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1253\/circj.CJ-11-1303","article-title":"Impact of diabetes mellitus on coronary atherosclerosis and plaque composition under statin therapy\u2014subanalysis of the TRUTH study","volume":"76","author":"T Nozue","year":"2012","journal-title":"Circulation journal: official journal of the Japanese Circulation Society"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.cmet.2013.04.001","article-title":"Hyperglycemia promotes myelopoiesis and impairs the resolution of atherosclerosis","volume":"17","author":"PR Nagareddy","year":"2013","journal-title":"Cell metabolism"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1210\/jc.2004-0372","article-title":"Obesity, metabolic syndrome, and cardiovascular disease","volume":"89","author":"SM Grundy","year":"2004","journal-title":"J Clin Endocrinol Metab"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1067\/mva.2001.115963","article-title":"Dramatic remodeling of advanced atherosclerotic plaques of the apolipoprotein E-deficient mouse in a novel transplantation model","volume":"34","author":"ED Reis","year":"2001","journal-title":"J Vasc Surg"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0022-4804(03)00039-8","article-title":"Mouse model of heterotopic aortic arch transplantation","volume":"111","author":"I Chereshnev","year":"2003","journal-title":"J Surg Res"},{"issue":"9","key":"ref7","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1161\/01.CIR.0000054781.50889.0C","article-title":"Eliminating atherogenesis in mice by switching off hepatic lipoprotein secretion","volume":"107","author":"HD Lieu","year":"2003","journal-title":"Circulation"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.2337\/db10-0778","article-title":"Diabetes adversely affects macrophages during atherosclerotic plaque regression in mice","volume":"60","author":"S Parathath","year":"2011","journal-title":"Diabetes"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s00109-011-0722-x","article-title":"Regression and stabilization of advanced murine atherosclerotic lesions: a comparison of LDL lowering and HDL raising gene transfer strategies","volume":"89","author":"E Van Craeyveld","year":"2011","journal-title":"Journal of molecular medicine (Berlin, Germany)"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/sj.gt.3301615","article-title":"Inhibition of atherosclerosis in apolipoprotein-E-deficient mice following muscle transduction with adeno-associated virus vectors encoding human apolipoprotein-E","volume":"9","author":"JD Harris","year":"2002","journal-title":"Gene therapy"},{"issue":"17","key":"ref11","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1161\/01.CIR.100.17.1816","article-title":"Regression of atherosclerosis induced by liver-directed gene transfer of apolipoprotein A-I in mice","volume":"100","author":"RK Tangirala","year":"1999","journal-title":"Circulation"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/S0022-2275(20)31618-7","article-title":"Effects of coexpression of the LDL receptor and apoE on cholesterol metabolism and atherosclerosis in LDL receptor-deficient mice","volume":"42","author":"M-a Kawashiri","year":"2001","journal-title":"Journal of Lipid Research"},{"issue":"5","key":"ref13","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1172\/JCI43802","article-title":"Suppressed monocyte recruitment drives macrophage removal from atherosclerotic plaques of Apoe-\/- mice during disease regression","volume":"121","author":"S Potteaux","year":"2011","journal-title":"J Clin Invest"},{"key":"ref14","first-page":"106","article-title":"Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia","volume":"2","author":"R Li","year":"2011","journal-title":"J Genet Syndr Gene Ther"},{"issue":"20","key":"ref15","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1038\/sj.gt.3302310","article-title":"Low-density lipoprotein receptor gene therapy using helper-dependent adenovirus produces long-term protection against atherosclerosis in a mouse model of familial hypercholesterolemia","volume":"11","author":"S Nomura","year":"2004","journal-title":"Gene therapy"},{"issue":"22","key":"ref16","doi-asserted-by":"crossref","first-page":"12816","DOI":"10.1073\/pnas.96.22.12816","article-title":"Administration of helper-dependent adenoviral vectors and sequential delivery of different vector serotype for long-term liver-directed gene transfer in baboons","volume":"96","author":"N Morral","year":"1999","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"23","key":"ref17","doi-asserted-by":"crossref","first-page":"13282","DOI":"10.1073\/pnas.241506298","article-title":"Lifetime correction of genetic deficiency in mice with a single injection of helper-dependent adenoviral vector","volume":"98","author":"IH Kim","year":"2001","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s11883-004-0033-6","article-title":"Liver-directed gene therapy for dyslipidemia and diabetes","volume":"6","author":"K Oka","year":"2004","journal-title":"Curr Atheroscler Rep"},{"key":"ref19","first-page":"329","article-title":"Construction and characterization of helper-dependent adenoviral vectors for sustained in vivo gene therapy","volume":"108","author":"K Oka","year":"2005","journal-title":"Methods Mol Med"},{"issue":"10","key":"ref20","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1161\/01.ATV.0000090126.34881.B1","article-title":"Induction of atherosclerosis by low-fat, semisynthetic diets in LDL receptor-deficient C57BL\/6J and FVB\/NJ mice: comparison of lesions of the aortic root, brachiocephalic artery, and whole aorta (en face measurement)","volume":"23","author":"D Teupser","year":"2003","journal-title":"Arteriosclerosis, thrombosis, and vascular biology"},{"key":"ref21","unstructured":"Brosius F. Low-Dose Streptozotocin Induction Protocol (Mouse). Animal Models of Diabetic Complications Consortium. 2003;www.diacomp.org\/shared\/showFile.aspx?doctypeid=3&docid=19<\/ext-link>, accessed May 19, 2014 (http:\/\/www.diacomp.org\/shared\/showFile.aspx?doctypeid=3&docid=19<\/ext-link>)."},{"issue":"10","key":"ref22","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1161\/01.RES.0000266449.37396.1f","article-title":"Recipes for creating animal models of diabetic cardiovascular disease","volume":"100","author":"W Hsueh","year":"2007","journal-title":"Circ Res"},{"issue":"12","key":"ref23","doi-asserted-by":"crossref","first-page":"E715","DOI":"10.1073\/pnas.1111600109","article-title":"Diabetes promotes an inflammatory macrophage phenotype and atherosclerosis through acyl-CoA synthetase 1","volume":"109","author":"JE Kanter","year":"2012","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1172\/JCI200417867","article-title":"Diabetes and diabetes-associated lipid abnormalities have distinct effects on initiation and progression of atherosclerotic lesions","volume":"114","author":"CB Renard","year":"2004","journal-title":"The Journal of Clinical Investigation"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/978-1-60327-369-5_5","article-title":"Laser capture microdissection for analysis of macrophage gene expression from atherosclerotic lesions","volume":"1027","author":"JE Feig","year":"2013","journal-title":"Methods in molecular biology (Clifton, NJ)"},{"issue":"7","key":"ref26","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1161\/ATVBAHA.108.179739","article-title":"Concept of vulnerable\/unstable plaque","volume":"30","author":"AV Finn","year":"2010","journal-title":"Arteriosclerosis, thrombosis, and vascular biology"},{"issue":"10","key":"ref27","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1073\/pnas.0511043103","article-title":"Gene expression changes in foam cells and the role of chemokine receptor CCR7 during atherosclerosis regression in ApoE-deficient mice","volume":"103","author":"E Trogan","year":"2006","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"17","key":"ref28","doi-asserted-by":"crossref","first-page":"7166","DOI":"10.1073\/pnas.1016086108","article-title":"HDL promotes rapid atherosclerosis regression in mice and alters inflammatory properties of plaque monocyte-derived cells","volume":"108","author":"JE Feig","year":"2011","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1093\/cvr\/cvp017","article-title":"Mechanisms for increased myocardial fatty acid utilization following short-term high-fat feeding","volume":"82","author":"JJ Wright","year":"2009","journal-title":"Cardiovascular research"},{"issue":"suppl 3","key":"ref30","doi-asserted-by":"crossref","first-page":"S215","DOI":"10.2337\/diabetes.53.suppl_3.S215","article-title":"The High-Fat Diet\u2013Fed Mouse: A Model for Studying Mechanisms and Treatment of Impaired Glucose Tolerance and Type 2","volume":"53","author":"MS Winzell","year":"2004","journal-title":"Diabetes. Diabetes"},{"issue":"12","key":"ref31","doi-asserted-by":"crossref","first-page":"3530","DOI":"10.2337\/diabetes.54.12.3530","article-title":"Unraveling the temporal pattern of diet-induced insulin resistance in individual organs and cardiac dysfunction in C57BL\/6 mice","volume":"54","author":"SY Park","year":"2005","journal-title":"Diabetes"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3945\/jn.108.098269","article-title":"Saturated Fatty Acid-Mediated Inflammation and Insulin Resistance in Adipose Tissue: Mechanisms of Action and Implications","volume":"139","author":"A Kennedy","year":"2009","journal-title":"The Journal of Nutrition"},{"issue":"1\u20132","key":"ref33","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1023\/A:1023423005187","article-title":"High levels of palmitic acid lead to insulin resistance due to changes in the level of phosphorylation of the insulin receptor and insulin receptor substrate-1","volume":"246","author":"R Reynoso","year":"2003","journal-title":"Molecular and cellular biochemistry"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.abb.2003.08.020","article-title":"Characterizing the effects of saturated fatty acids on insulin signaling and ceramide and diacylglycerol accumulation in 3T3-L1 adipocytes and C2C12 myotubes","volume":"419","author":"JA Chavez","year":"2003","journal-title":"Archives of biochemistry and biophysics"},{"issue":"10","key":"ref35","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/S2213-8587(14)70146-9","article-title":"Differences in the prospective association between individual plasma phospholipid saturated fatty acids and incident type 2 diabetes: the EPIC-InterAct case-cohort study","volume":"2","author":"NG Forouhi","year":"2014","journal-title":"The Lancet Diabetes & Endocrinology"},{"issue":"9","key":"ref36","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1161\/CIRCULATIONAHA.110.984146","article-title":"Reversal of hyperlipidemia with a genetic switch favorably affects the content and inflammatory state of macrophages in atherosclerotic plaques","volume":"123","author":"JE Feig","year":"2011","journal-title":"Circulation"},{"issue":"6","key":"ref37","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.3945\/ajcn.111.018846","article-title":"Palm olein increases plasma cholesterol moderately compared with olive oil in healthy individuals","volume":"94","author":"T Tholstrup","year":"2011","journal-title":"Am J Clin Nutr"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1146\/annurev.nu.13.070193.002035","article-title":"Regulation of plasma LDL-cholesterol levels by dietary cholesterol and fatty acids","volume":"13","author":"DK Spady","year":"1993","journal-title":"Annual review of nutrition"},{"issue":"10","key":"ref39","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1161\/01.ATV.0000092328.66882.F5","article-title":"Apolipoprotein A-I deficiency results in markedly increased atherosclerosis in mice lacking the LDL receptor","volume":"23","author":"RE Moore","year":"2003","journal-title":"Arteriosclerosis, thrombosis, and vascular biology"},{"issue":"9","key":"ref40","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1172\/JCI24819","article-title":"Human aldose reductase expression accelerates diabetic atherosclerosis in transgenic mice","volume":"115","author":"RK Vikramadithyan","year":"2005","journal-title":"J Clin Invest"},{"issue":"6","key":"ref41","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.2337\/db08-0083","article-title":"Decreased lipoprotein clearance is responsible for increased cholesterol in LDL receptor knockout mice with streptozotocin-induced diabetes","volume":"57","author":"IJ Goldberg","year":"2008","journal-title":"Diabetes"},{"issue":"6","key":"ref42","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1016\/j.ajpath.2013.08.019","article-title":"Hyperglycemia impairs atherosclerosis regression in mice","volume":"183","author":"N Gaudreault","year":"2013","journal-title":"Am J Pathol"},{"issue":"6","key":"ref43","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1073\/pnas.0709958105","article-title":"Type 1 diabetes promotes disruption of advanced atherosclerotic lesions in LDL receptor-deficient mice","volume":"105","author":"F Johansson","year":"2008","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"10","key":"ref44","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1038\/nri3520","article-title":"Macrophages in atherosclerosis: a dynamic balance","volume":"13","author":"KJ Moore","year":"2013","journal-title":"Nature reviews Immunology"},{"issue":"6","key":"ref45","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nri2784","article-title":"Unravelling mononuclear phagocyte heterogeneity","volume":"10","author":"F Geissmann","year":"2010","journal-title":"Nature reviews Immunology"},{"issue":"2","key":"ref46","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.cmet.2007.06.010","article-title":"PPARgamma activation primes human monocytes into alternative M2 macrophages with anti-inflammatory properties","volume":"6","author":"MA Bouhlel","year":"2007","journal-title":"Cell metabolism"},{"issue":"1","key":"ref47","doi-asserted-by":"crossref","first-page":"e8852","DOI":"10.1371\/journal.pone.0008852","article-title":"Macrophage plasticity in experimental atherosclerosis","volume":"5","author":"J Khallou-Laschet","year":"2010","journal-title":"PLoS One"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1479-5876-5-11","article-title":"Gene expression profiling of cutaneous wound healing","volume":"5","author":"K Deonarine","year":"2007","journal-title":"J Transl Med"},{"issue":"12","key":"ref49","doi-asserted-by":"crossref","first-page":"e28534","DOI":"10.1371\/journal.pone.0028534","article-title":"Statins promote the regression of atherosclerosis via activation of the CCR7-dependent emigration pathway in macrophages","volume":"6","author":"JE Feig","year":"2011","journal-title":"PLoS One"},{"issue":"5","key":"ref50","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.cmet.2013.10.002","article-title":"LXRs regulate ER stress and inflammation through dynamic modulation of membrane phospholipid composition","volume":"18","author":"X Rong","year":"2013","journal-title":"Cell metabolism"},{"issue":"8","key":"ref51","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1161\/ATVBAHA.109.191197","article-title":"Liver x receptor signaling pathways and atherosclerosis","volume":"30","author":"AC Calkin","year":"2010","journal-title":"Arteriosclerosis, thrombosis, and vascular biology"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0128996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T10:10:30Z","timestamp":1652263830000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0128996"}},"subtitle":[],"editor":[{"given":"Alberico","family":"Catapano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,6,5]]},"references-count":51,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,6,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0128996","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,5]]}}}