{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:24:02Z","timestamp":1792185842626,"version":"4.4.0"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T00:00:00Z","timestamp":1435622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0130713","type":"journal-article","created":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T18:52:50Z","timestamp":1435690370000},"page":"e0130713","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":49,"title":["Neisseria gonorrhoeae Modulates Immunity by Polarizing Human Macrophages to a M2 Profile"],"prefix":"10.1371","volume":"10","author":[{"given":"Mar\u00eda Carolina","family":"Ortiz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Lefimil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula I.","family":"Rodas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolando","family":"Vernal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Acu\u00f1a-Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Imarai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Escobar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,6,30]]},"reference":[{"key":"ref1","unstructured":"WHO. Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. 2012.1"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1056\/NEJM197401172900301","article-title":"Asymptomatic gonorrhea in men. Diagnosis, natural course, prevalence and significance","volume":"290","author":"HH Handsfield","year":"1974","journal-title":"N Engl J Med"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1097\/00126334-200308150-00014","article-title":"The value of screening for sexually transmitted diseases in an HIV clinic","volume":"33","author":"TA Farley","year":"2003","journal-title":"J Acquir Immune Defic Syndr"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1136\/sti.75.1.3","article-title":"From epidemiological synergy to public health policy and practice: the contribution of other sexually transmitted diseases to sexual transmission of HIV infection","volume":"75","author":"DT Fleming","year":"1999","journal-title":"Sex Transm Infect"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/mi.2009.139","article-title":"Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection","volume":"3","author":"B Feinen","year":"2010","journal-title":"Mucosal Immunol"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1093\/oxfordjournals.aje.a009820","article-title":"Longitudinal evaluation of serovar-specific immunity to Neisseria gonorrhoeae","volume":"149","author":"KK Fox","year":"1999","journal-title":"Am J Epidemiol"},{"issue":"8","key":"ref7","doi-asserted-by":"crossref","first-page":"3937","DOI":"10.1128\/IAI.67.8.3937-3946.1999","article-title":"Limited local and systemic antibody responses to Neisseria gonorrhoeae during uncomplicated genital infections","volume":"67","author":"SR Hedges","year":"1999","journal-title":"Infect Immun"},{"issue":"12","key":"ref8","doi-asserted-by":"crossref","first-page":"5456","DOI":"10.1128\/IAI.00552-08","article-title":"Regulatory T cells are locally induced during intravaginal infection of mice with Neisseria gonorrhoeae","volume":"76","author":"M Imarai","year":"2008","journal-title":"Infect Immun"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/mi.2012.12","article-title":"Neisseria gonorrhoeae selectively suppresses the development of Th1 and Th2 cells, and enhances Th17 cell responses, through TGF-beta-dependent mechanisms","volume":"5","author":"Y Liu","year":"2012","journal-title":"Mucosal Immunol"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Liu Y, Liu W, Russell MW. Suppression of host adaptive immune responses by Neisseria gonorrhoeae: role of interleukin 10 and type 1 regulatory T cells. Mucosal Immunol. 2013 Jun 12.348","DOI":"10.1038\/mi.2013.36"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1128\/IAI.74.1.442-448.2006","article-title":"Type IV pili of Neisseria gonorrhoeae influence the activation of human CD4+ T cells","volume":"74","author":"LJ Plant","year":"2006","journal-title":"Infect Immun"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/ni769","article-title":"Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes","volume":"3","author":"IC Boulton","year":"2002","journal-title":"Nat Immunol"},{"issue":"10","key":"ref13","doi-asserted-by":"crossref","first-page":"6827","DOI":"10.4049\/jimmunol.180.10.6827","article-title":"CEACAM1 dynamics during neisseria gonorrhoeae suppression of CD4+ T lymphocyte activation","volume":"180","author":"HS Lee","year":"2008","journal-title":"J Immunol"},{"issue":"7","key":"ref14","doi-asserted-by":"crossref","first-page":"e41260","DOI":"10.1371\/journal.pone.0041260","article-title":"Neisseria gonorrhoeae Suppresses Dendritic Cell-Induced, Antigen-Dependent CD4 T Cell Proliferation","volume":"7","author":"W Zhu","year":"2012","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"127017","DOI":"10.1155\/2013\/127017","article-title":"Neisseria gonorrhoeae Induces a Tolerogenic Phenotype in Macrophages to Modulate Host Immunity","volume":"2013","author":"A Escobar","year":"2013","journal-title":"Mediators Inflamm"},{"issue":"10","key":"ref16","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1038\/ni.1937","article-title":"Macrophage plasticity and interaction with lymphocyte subsets: cancer as a paradigm","volume":"11","author":"SK Biswas","year":"2010","journal-title":"Nat Immunol"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1172\/JCI59643","article-title":"Macrophage plasticity and polarization: in vivo veritas","volume":"122","author":"A Sica","year":"2012","journal-title":"J Clin Invest"},{"issue":"7446","key":"ref18","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/nature12034","article-title":"Macrophage biology in development, homeostasis and disease","volume":"496","author":"TA Wynn","year":"2013","journal-title":"Nature"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1002\/eji.200738067","article-title":"Coxiella burnetii, the agent of Q fever, stimulates an atypical M2 activation program in human macrophages","volume":"38","author":"M Benoit","year":"2008","journal-title":"Eur J Immunol"},{"issue":"3","key":"ref20","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1097\/QCO.0b013e328344b73e","article-title":"Macrophage polarization and bacterial infections","volume":"24","author":"JL Mege","year":"2011","journal-title":"Curr Opin Infect Dis"},{"issue":"10","key":"ref21","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1111\/j.1348-0421.2007.tb03986.x","article-title":"Pattern of macrophage activation in yersinia-resistant and yersinia-susceptible strains of mice","volume":"51","author":"AR Tumitan","year":"2007","journal-title":"Microbiol Immunol"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1111\/j.1365-2249.2006.03020.x","article-title":"The local immune response in ulcerative lesions of Buruli disease","volume":"143","author":"AE Kiszewski","year":"2006","journal-title":"Clin Exp Immunol"},{"issue":"10","key":"ref23","doi-asserted-by":"crossref","first-page":"6460","DOI":"10.4049\/jimmunol.0802696","article-title":"Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome","volume":"182","author":"JA Duncan","year":"2009","journal-title":"J Immunol"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1046\/j.1365-2958.2000.01694.x","article-title":"Interaction of primary human endometrial cells with Neisseria gonorrhoeae expressing green fluorescent protein","volume":"35","author":"M Christodoulides","year":"2000","journal-title":"Mol Microbiol"},{"issue":"9","key":"ref25","doi-asserted-by":"crossref","first-page":"3857","DOI":"10.1128\/IAI.65.9.3857-3866.1997","article-title":"Binding of vitronectin to opa-expressing Neisseria gonorrhoeae mediates invasion of HeLa cells","volume":"65","author":"OG Gomez-Duarte","year":"1997","journal-title":"Infect Immun"},{"issue":"10","key":"ref26","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.archoralbio.2008.04.004","article-title":"Expression of proinflammatory cytokines in osteoarthritis of the temporomandibular joint","volume":"53","author":"R Vernal","year":"2008","journal-title":"Arch Oral Biol"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1189\/jlb.1108702","article-title":"GM-CSF- and M-CSF-dependent macrophage phenotypes display differential dependence on type I interferon signaling","volume":"86","author":"AJ Fleetwood","year":"2009","journal-title":"J Leukoc Biol"},{"issue":"6","key":"ref28","doi-asserted-by":"crossref","first-page":"3733","DOI":"10.4049\/jimmunol.181.6.3733","article-title":"Macrophage polarization in bacterial infections","volume":"181","author":"M Benoit","year":"2008","journal-title":"J Immunol"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.immuni.2007.05.016","article-title":"Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses","volume":"27","author":"MJ Butte","year":"2007","journal-title":"Immunity"},{"issue":"5","key":"ref30","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/nri1349","article-title":"Co-inhibitory molecules of the B7-CD28 family in the control of T-cell immunity","volume":"4","author":"L Chen","year":"2004","journal-title":"Nat Rev Immunol"},{"issue":"7","key":"ref31","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1093\/intimm\/dxm057","article-title":"PD-1 and PD-1 ligands: from discovery to clinical application","volume":"19","author":"T Okazaki","year":"2007","journal-title":"Int Immunol"},{"issue":"12","key":"ref32","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1038\/70932","article-title":"B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion","volume":"5","author":"H Dong","year":"1999","journal-title":"Nat Med"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1002\/0470871628.ch6","article-title":"CD4+CD25+ regulatory cells from human peripheral blood express very high levels of CD25 ex vivo","volume":"252","author":"C Baecher-Allan","year":"2003","journal-title":"Novartis Found Symp"},{"issue":"10","key":"ref34","doi-asserted-by":"crossref","first-page":"6265","DOI":"10.4049\/jimmunol.175.10.6265","article-title":"Murine vascular endothelium activates and induces the generation of allogeneic CD4+25+Foxp3+ regulatory T cells","volume":"175","author":"AS Krupnick","year":"2005","journal-title":"J Immunol"},{"issue":"13","key":"ref35","doi-asserted-by":"crossref","first-page":"3015","DOI":"10.1084\/jem.20090847","article-title":"PD-L1 regulates the development, maintenance, and function of induced regulatory T cells","volume":"206","author":"LM Francisco","year":"2009","journal-title":"J Exp Med"},{"issue":"12","key":"ref36","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.it.2004.09.015","article-title":"The chemokine system in diverse forms of macrophage activation and polarization","volume":"25","author":"A Mantovani","year":"2004","journal-title":"Trends Immunol"},{"issue":"7","key":"ref37","doi-asserted-by":"crossref","first-page":"3508","DOI":"10.4049\/jimmunol.1200662","article-title":"Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction","volume":"189","author":"G Zizzo","year":"2012","journal-title":"J Immunol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1742-2094-10-35","article-title":"Macrophages in inflammatory multiple sclerosis lesions have an intermediate activation status","volume":"10","author":"DY Vogel","year":"2013","journal-title":"J Neuroinflammation"},{"issue":"5","key":"ref39","doi-asserted-by":"crossref","first-page":"3287","DOI":"10.4049\/jimmunol.175.5.3287","article-title":"TNF-alpha-dependent and-independent maturation of dendritic cells and recruited CD11c(int)CD11b+ Cells during oral Salmonella infection","volume":"175","author":"M Sundquist","year":"2005","journal-title":"J Immunol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1186\/1756-0500-4-247","article-title":"Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis","volume":"4","author":"A Mihret","year":"2011","journal-title":"BMC Res Notes"},{"issue":"12","key":"ref41","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1038\/nri1248","article-title":"CD28-mediated co-stimulation: a quantitative support for TCR signalling","volume":"3","author":"O Acuto","year":"2003","journal-title":"Nat Rev Immunol"},{"issue":"6","key":"ref42","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.4049\/jimmunol.169.6.3155","article-title":"Toll-like receptor 4 expression is required to control chronic Mycobacterium tuberculosis infection in mice","volume":"169","author":"B Abel","year":"2002","journal-title":"J Immunol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1146\/annurev.iy.09.040191.002325","article-title":"Fc receptors","volume":"9","author":"JV Ravetch","year":"1991","journal-title":"Annu Rev Immunol"},{"issue":"9","key":"ref44","doi-asserted-by":"crossref","first-page":"676","DOI":"10.3390\/toxins4090676","article-title":"Macrophage-targeted therapy: CD64-based immunotoxins for treatment of chronic inflammatory diseases","volume":"4","author":"D Hristodorov","year":"2012","journal-title":"Toxins (Basel)"},{"issue":"1","key":"ref45","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1128\/IAI.00711-09","article-title":"Mouse strain-dependent differences in susceptibility to Neisseria gonorrhoeae infection and induction of innate immune responses","volume":"78","author":"M Packiam","year":"2010","journal-title":"Infect Immun"},{"issue":"3","key":"ref46","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nri2711","article-title":"The regulation of IL-10 production by immune cells","volume":"10","author":"M Saraiva","year":"2010","journal-title":"Nat Rev Immunol"},{"issue":"2","key":"ref47","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1084\/jem.20050019","article-title":"A critical role for the programmed death ligand 1 in fetomaternal tolerance","volume":"202","author":"I Guleria","year":"2005","journal-title":"J Exp Med"},{"issue":"9","key":"ref48","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1093\/rheumatology\/ken256","article-title":"Active systemic lupus erythematosus is associated with failure of antigen-presenting cells to express programmed death ligand-1","volume":"47","author":"N Mozaffarian","year":"2008","journal-title":"Rheumatology (Oxford)"},{"issue":"4","key":"ref49","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.4049\/jimmunol.174.4.1888","article-title":"Prevention of experimental autoimmune encephalomyelitis by transfer of embryonic stem cell-derived dendritic cells expressing myelin oligodendrocyte glycoprotein peptide along with TRAIL or programmed death-1 ligand","volume":"174","author":"S Hirata","year":"2005","journal-title":"J Immunol"},{"issue":"2\u20133","key":"ref50","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.clim.2005.10.017","article-title":"Delivering PD-1 inhibitory signal concomitant with blocking ICOS co-stimulation suppresses lupus-like syndrome in autoimmune BXSB mice","volume":"118","author":"H Ding","year":"2006","journal-title":"Clin Immunol"},{"issue":"24","key":"ref51","doi-asserted-by":"crossref","first-page":"5640","DOI":"10.1182\/blood-2012-01-380121","article-title":"Properties of mouse and human IgG receptors and their contribution to disease models","volume":"119","author":"P Bruhns","year":"2012","journal-title":"Blood"},{"issue":"6","key":"ref52","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1046\/j.1365-2133.2000.03534.x","article-title":"Expression of Fc receptors for IgG during acute and chronic cutaneous inflammation in atopic dermatitis","volume":"142","author":"RC Kiekens","year":"2000","journal-title":"Br J Dermatol"},{"issue":"4","key":"ref53","doi-asserted-by":"crossref","first-page":"e32588","DOI":"10.1371\/journal.pone.0032588","article-title":"Spherical lactic acid bacteria activate plasmacytoid dendritic cells immunomodulatory function via TLR9-dependent crosstalk with myeloid dendritic cells","volume":"7","author":"K Jounai","year":"2012","journal-title":"PLoS One"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1186\/1471-2334-11-347","article-title":"Enhanced upper genital tract pathologies by blocking Tim-3 and PD-L1 signaling pathways in mice intravaginally infected with Chlamydia muridarum","volume":"11","author":"B Peng","year":"2011","journal-title":"BMC Infect Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0130713","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T09:21:30Z","timestamp":1599211290000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0130713"}},"subtitle":[],"editor":[{"given":"Michal A","family":"Olszewski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,6,30]]},"references-count":54,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,6,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0130713","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,30]]}}}