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glycoprotein Ib-IX complex: studies using optical tweezers","volume":"99","author":"M Arya","year":"2002","journal-title":"Blood"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"28633","DOI":"10.1074\/jbc.273.44.28633","article-title":"Laminin-1 and Laminin-2 G-domain Synthetic Peptides Bind Syndecan-1 and Are Involved in Acinar Formation of a Human Submandibular Gland Cell Line","volume":"273","author":"MP Hoffman","year":"1998","journal-title":"J Biol Chem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1161\/CIRCRESAHA.108.187831","article-title":"The glycocalyx of the human umbilical vein endothelial cell: an impressive structure ex vivo but not in culture","volume":"104","author":"D Chappell","year":"2009","journal-title":"Circ Res"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1083\/jcb.200404171","article-title":"The syndecan-1 ectodomain regulates alphavbeta3 integrin activity in human mammary carcinoma cells","volume":"167","author":"DM Beauvais","year":"2004","journal-title":"J Cell Biol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1038\/nrm2289","article-title":"Synergistic control of cell adhesion by integrins and syndecans","volume":"8","author":"MR Morgan","year":"2007","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.cell.2006.06.044","article-title":"Matrix elasticity directs stem cell lineage specification","volume":"126","author":"AJ Engler","year":"2006","journal-title":"Cell"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1161\/01.RES.0000224114.65109.4e","article-title":"Extracellular matrix remodeling and organization in developing and diseased aortic valves","volume":"98","author":"RB Hinton","year":"2006","journal-title":"Circ 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