{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:36:07Z","timestamp":1792985767296,"version":"4.5.0"},"reference-count":17,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T00:00:00Z","timestamp":1435104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0130986","type":"journal-article","created":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T18:12:40Z","timestamp":1435169560000},"page":"e0130986","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Delivery of Topically Applied Calpain Inhibitory Peptide to the Posterior Segment of the Rat Eye"],"prefix":"10.1371","volume":"10","author":[{"given":"Taku","family":"Ozaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Nakazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sei-ichi","family":"Ishiguro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,6,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.preteyeres.2010.04.001","article-title":"Protein delivery for retinal diseases: From basic considerations to clinical applications","volume":"29","author":"El Sanharawi","year":"2010","journal-title":"Prog Ret Eye Res"},{"key":"ref2","first-page":"797","article-title":"Accumulation of topically applied porcine insulin in the retina and optic nerve in normal and diabetic rats","volume":"43","author":"SB Koevary","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3800","DOI":"10.1167\/iovs.05-0301","article-title":"Pharmacokinetics of conjunctivally applied nerve growth factor in the retina and optic nerve of adult rats","volume":"46","author":"A Lambiase","year":"2005","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1038\/sj.eye.6701669","article-title":"Topically applied antibody fragments penetrate into the back of the rabbit eye","volume":"19","author":"KA Williams","year":"2005","journal-title":"Eye"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1167\/iovs.08-2370","article-title":"Pharmacokinetics and posterior segment biodistribution of ESBA105, an anti-TNF-\u03b1 single-chain antibody, upon topical administration to the rabbit eye","volume":"50","author":"E Furrer","year":"2009","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref6","first-page":"1783","article-title":"Intravitreal or topical eye-drop application of a \u03bc-calpain C2L domain peptide protects against photoreceptor cell death in Royal College of Surgeons rats, a model of retinitis pigmentosa","volume":"1822","author":"T Ozaki","year":"2012","journal-title":"Biochem Biophys Acta"},{"key":"ref7","first-page":"1","article-title":"Inhibitory peptide of mitochondrial \u03bc-calpain protects against photoreceptor degeneration in rhodopsin transgenic S334ter and P23H rats","volume":"2013","author":"T Ozaki","year":"2013","journal-title":"PLoS One"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.exer.2010.06.004","article-title":"Activation of mitochondrial calpain and release of apoptosis-inducing factor from mitochondria in RCS rat retinal degeneration","volume":"91","author":"S Mizukoshi","year":"2010","journal-title":"Exp Eye Res"},{"key":"ref9","first-page":"3154","article-title":"Expression of glial fibrillary acidic protein in rabbit M\u00fcller cells after lensectomy-vitrectomy","volume":"34","author":"A Yoshida","year":"1993","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"suppl. 1","key":"ref10","doi-asserted-by":"crossref","first-page":"S12","DOI":"10.2337\/diabetes.53.2007.S12","article-title":"Structure, activation, and biology of calpain","volume":"53","author":"K Suzuki","year":"2004","journal-title":"Diabetes"},{"key":"ref11","first-page":"523","article-title":"Demonstration of three calpains in the matrix of rat liver mitochondria","volume":"50","author":"A Tavares","year":"1991","journal-title":"Biomed Biochim Acta"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S0167-4889(99)00062-2","article-title":"Possible mechanism for the decrease of mitochondrial aspartate aminotransferase activity in ischemic and hypoxic rat retinas","volume":"1450","author":"S Endo","year":"1999","journal-title":"Biochem Biophys Acta"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1093\/jb\/mvm143","article-title":"Characteristics of mitochondrial calpains","volume":"142","author":"T Ozaki","year":"2007","journal-title":"J Biochem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1016\/j.bbamcr.2008.05.011","article-title":"ERp57-associated mitochondrial \u03bc-calpain truncates apoptosis-inducing factor","volume":"1783","author":"T Ozaki","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.abb.2010.12.003","article-title":"Ca<sup>2+<\/sup>-induced release of mitochondrial m-calpain from other membrane with binding of calpain small subunit and Grp 75","volume":"507","author":"T Ozaki","year":"2011","journal-title":"Arch Biochem Biophys"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1093\/cvr\/cvs232","article-title":"Contribution of calpains to myocardial ishcaemia\/reperfusion injury","volume":"96","author":"J Inserte","year":"2012","journal-title":"Cardiovas Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1247\/csf.13008","article-title":"Decrease of ATP by mitochondrial m-calpain inhibitory peptide in the rat retinas","volume":"38","author":"T Ozaki","year":"2013","journal-title":"Cell Struct Funct"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0130986","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T13:55:14Z","timestamp":1540216514000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0130986"}},"subtitle":[],"editor":[{"given":"Rong","family":"Wen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,6,24]]},"references-count":17,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,6,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0130986","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,24]]}}}