{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T12:57:49Z","timestamp":1770382669518,"version":"3.49.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T00:00:00Z","timestamp":1435276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0131364","type":"journal-article","created":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T18:17:49Z","timestamp":1435342669000},"page":"e0131364","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of Different Adjuvants on Protection and Side-Effects Induced by Helicobacter suis Whole-Cell Lysate Vaccination"],"prefix":"10.1371","volume":"10","author":[{"given":"Iris","family":"Bosschem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jagadeesh","family":"Bayry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"De Bruyne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Van Deun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemieke","family":"Smet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Griet","family":"Vercauteren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Ducatelle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Freddy","family":"Haesebrouck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bram","family":"Flahou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,6,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1136\/vr.161.6.189","article-title":"Prevalence of \u2018<italic>Candidatus<\/italic> Helicobacter suis\u2019 in pigs of different ages","volume":"161","author":"A Hellemans","year":"2007","journal-title":"Veterinary record"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.vetmic.2012.06.031","article-title":"An experimental <italic>Helicobacter suis<\/italic> infection causes gastritis and reduced daily weight gain in pigs","volume":"160","author":"E De Bruyne","year":"2012","journal-title":"Veterinary Microbiology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1128\/CMR.00041-08","article-title":"Gastric <italic>Helicobacters<\/italic> in domestic animals and non-human primates and their significance for human health","volume":"22","author":"F Haesebrouck","year":"2009","journal-title":"Clinical Microbiology Reviews"},{"key":"ref4","first-page":"167","article-title":"<italic>Helicobacter pylori<\/italic> in the 21<sup>st<\/sup> century","author":"TG Blanchard","year":"2010"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Davies MN, Bayry J, Tchilian EZ, Vani J, Shaila MS, Forbes EK, et al. (2009) Toward the Discovery of Vaccine Adjuvants: Coupling <italic>In Silico<\/italic> Screening and <italic>In Vitro<\/italic> Analysis of Antagonist Binding to Human and Mouse CCR4 Receptors. PLoS One. <comment>doi: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1371\/journal.pone.0008084\" xlink:type=\"simple\">10.1371\/journal.pone.0008084<\/ext-link><\/comment>","DOI":"10.1371\/journal.pone.0008084"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0016-5085(95)90275-9","article-title":"Oral immunization with <italic>Helicobacter pylori<\/italic> urease B subunit as a treatment against <italic>Helicobacter<\/italic> infection in mice","volume":"109","author":"I Corth\u00e9sy-Theulaz","year":"1995","journal-title":"Gastroenterology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1136\/gut.51.5.634","article-title":"Safety and efficacy of low dose <italic>Escherichia coli<\/italic> enterotoxin adjuvant for urease based oral immunization against <italic>Helicobacter pylori<\/italic> in healthy volunteers,\u201d","volume":"51","author":"S Banerjee","year":"2002","journal-title":"Gut"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3529","DOI":"10.1128\/IAI.70.7.3529-3538.2002","article-title":"Clearance of <italic>Helicobacter pylori<\/italic> infection and resolution of post-immunization gastritis in a kinetic study of prophylactically immunized mice","volume":"70","author":"CA Garhart","year":"2002","journal-title":"Infection and Immunity"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/S0264-410X(97)00258-2","article-title":"Systemic immunization with urease protects mice against <italic>Helicobacter pylori<\/italic> infection","volume":"16","author":"B Guy","year":"1998","journal-title":"Vaccine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1016\/S0264-410X(98)00332-6","article-title":"Comparison between targeted and untargeted systemic immunizations with adjuvanted urease to cure <italic>Helicobacter pylori<\/italic> infection in mice","volume":"17","author":"B Guy","year":"1999","journal-title":"Vaccine"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.micinf.2005.07.010","article-title":"Mucosal immune responses are related to the reduction of bacterial colonization in the stomach after therapeutic <italic>Helicobacter pylori<\/italic> immunization in mice","volume":"8","author":"J Nystr\u00f6m","year":"2006","journal-title":"Microbes and Infection"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1574-695X.2006.00206.x","article-title":"Effectiveness of vaccination with recombinant HpaA from <italic>Helicobacter pylori<\/italic> is influenced by host genetic background","volume":"50","author":"P Sutton","year":"2007","journal-title":"FEMS Immunology and Medical Microbiology"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1574-695X.2007.00237.x","article-title":"Progress in vaccine development against <italic>Helicobacter pylori<\/italic>","volume":"50","author":"AM Svennerholm","year":"2007","journal-title":"FEMS Immunology and Medical Microbiology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.3748\/wjg.v13.i10.1547","article-title":"The immune response induced by <italic>Helicobacter pylori<\/italic> vaccine with chitosan as adjuvant and its relation to immune protection","volume":"13","author":"Y Xie","year":"2007","journal-title":"World Journal of Gastroenterology"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1128\/IAI.71.1.40-46.2003","article-title":"Mucosal immunization with <italic>Helicobacter<\/italic>, CpG DNA, and cholera toxin is protective","volume":"71","author":"W Jiang","year":"2003","journal-title":"Infection and Immunity"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"7014","DOI":"10.1128\/IAI.71.12.7014-7022.2003","article-title":"Orally administered CpG oligodeoxynucleotide induces production of CXC and CC chemokines in the gastric mucosa and suppresses bacterial colonization in a mouse model of <italic>Helicobacter pylori<\/italic> infection","volume":"71","author":"S Raghavan","year":"2003","journal-title":"Infection and Immunity"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"11939","DOI":"10.3748\/wjg.v20.i34.11939","article-title":"<italic>Helicobacter pylori<\/italic> vaccination: Is there a path to protection?","volume":"34","author":"F Anderl","year":"2014","journal-title":"World Journal of Gastroenterology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1016\/j.vaccine.2008.12.031","article-title":"Protective immunization with homologous and heterologous antigens against <italic>Helicobacter suis<\/italic> challenge in a mouse model","volume":"27","author":"B Flahou","year":"2009","journal-title":"Vaccine"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Vermoote M, Flahou B, Pasmans F, Ducatelle R, Haesebrouck F (2013) Protective efficacy of vaccines based on the <italic>Helicobacter suis<\/italic> urease subunit B and \u03b3-glutamyl transpeptidase. Vaccine. <comment>doi: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1016\/j.vaccine.2013.05.047\" xlink:type=\"simple\">10.1016\/j.vaccine.2013.05.047<\/ext-link><\/comment>","DOI":"10.1016\/j.vaccine.2013.05.047"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.tips.2014.02.003","article-title":"Targeting CCR4 as an emerging strategy for cancer therapy and vaccines","volume":"35","author":"J Bayry","year":"2014","journal-title":"Trends Pharmacol Sci"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"10221","DOI":"10.1073\/pnas.0803453105","article-title":"In silico identified CCR4 antagonists target regulatory T cells and exert adjuvant activity in vaccination","volume":"105","author":"J Bayry","year":"2008","journal-title":"Proc Natl Acad Sci. USA"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1084\/jem.20070081","article-title":"Altering the distribution of Foxp3(+) regulatory T cells results in tissue-specific inflammatory disease","volume":"204","author":"BD Sather","year":"2007","journal-title":"J Exp Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1084\/jem.187.1.129","article-title":"Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s","volume":"187","author":"R Bonecchi","year":"1998","journal-title":"J Exp Med"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.2353\/ajpath.2009.080987","article-title":"Surveillance of antigen-presenting cells by CD4+ CD25+ regulatory T cells in autoimmunity: immunopathogenesis and therapeutic implications","volume":"174","author":"S Andr\u00e9","year":"2009","journal-title":"Am J Pathol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"4853","DOI":"10.1182\/blood-2011-01-329656","article-title":"A CCR4 antagonist combined with vaccines induces antigen-specific CD8+ T cells and tumor immunity against self antigens","volume":"118","author":"H Pere","year":"2011","journal-title":"Blood"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1099\/ijs.0.65133-0","article-title":"Isolation and characterization of <italic>Helicobacter suis<\/italic> sp. nov. from pig stomachs","volume":"58","author":"M Baele","year":"2008","journal-title":"International Journal of Systematic and evolutionary Microbiology"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2332\/allergolint.09-OA-0103","article-title":"Curdlan induces DC-mediated Th17 polarization via Jagged1 activation in human dendritic cells","volume":"59","author":"T Higashi","year":"2010","journal-title":"Allergol Int"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/S0264-410X(96)00183-1","article-title":"Adjuvants\u2014a classification and review of their modes of action","volume":"15","author":"JC Cox","year":"1997","journal-title":"Vaccine"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1297-9716-43-75","article-title":"The local immune response of mice after <italic>Helicobacter suis<\/italic> infection: strain differences and distinction with <italic>Helicobacter pylori<\/italic>","volume":"43","author":"B Flahou","year":"2012","journal-title":"Veterinary Research"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1099\/ijs.0.63117-0","article-title":"Description of \u2018Candidatus Helicobacter heilmannii\u2019 based on DNA sequence analysis of 16S rRNA and urease genes","volume":"54","author":"JL O'Rourke","year":"2004","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2<sup>\u2212\u0394\u0394CT<\/sup> method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"9","key":"ref32","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1155\/2001\/367832","article-title":"The updated Sydney system: classification and grading of gastritis as the basis of diagnosis and treatment","volume":"15","author":"M Stolte","year":"2001","journal-title":"Can J Gastroenterol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1084\/jem.188.12.2277","article-title":"Immunization of mice with urease vaccine affords protection against <italic>Helicobacter pylori<\/italic> infection in the absence of antibodies and is mediated by MHC class-II restricted responses","volume":"188","author":"TH Ermak","year":"1998","journal-title":"The Journal of Experimental Medicine"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1128\/IAI.67.5.2531-2539.1999","article-title":"Local secretory immunoglobulin A and post-immunization gastritis correlate with protection against <italic>Helicobacter pylori<\/italic> infection after oral vaccination of mice","volume":"67","author":"T Goto","year":"1999","journal-title":"Infection and Immunity"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1099\/jmm.0.47061-0","article-title":"Effects of vaccination by a recombinant antigen ureB 138 (a segment of the \u03b2-subunit of urease) against <italic>Helicobacter pylori<\/italic> infection","volume":"56","author":"F Morihara","year":"2007","journal-title":"Journal of Medical Microbiology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1053\/j.gastro.2009.11.043","article-title":"<italic>Helicobacter pylori<\/italic> escape is mediated by dendritic cell-induced Treg skewing and Th17 suppression in mice,\u201d","volume":"138","author":"JK Kao","year":"2010","journal-title":"Gastroenterology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"3782","DOI":"10.4049\/jimmunol.170.7.3782","article-title":"Depletion of neutrophils in IL-10<sup>-\/-<\/sup> mice delays clearance of gastric <italic>Helicobacter<\/italic> infection and decreases the Th1 immune response to <italic>Helicobacter<\/italic>","volume":"170","author":"HF Ismail","year":"2003","journal-title":"Journal of Immunology"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/j.1523-5378.2005.00349.x","article-title":"Eradication of <italic>Helicobacter pylori<\/italic> and resolution of gastritis in the gastric mucosa of Il-10-deficient mice","volume":"10","author":"Y Matsumoto","year":"2005","journal-title":"Helicobacter"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1053\/j.gastro.2008.09.017","article-title":"Vaccination of mice against <italic>H<\/italic>. <italic>pylori<\/italic> induces a strong Th-17 response and immunity that is neutrophil dependent","volume":"136","author":"ES DeLyria","year":"2009","journal-title":"Gastroenterology"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0131364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T04:38:45Z","timestamp":1599194325000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0131364"}},"subtitle":[],"editor":[{"given":"Luciana C. C.","family":"Leite","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,6,26]]},"references-count":39,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,6,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0131364","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,26]]}}}