{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:36:25Z","timestamp":1777667785676,"version":"3.51.4"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T00:00:00Z","timestamp":1437436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0133558","type":"journal-article","created":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T18:18:36Z","timestamp":1437502716000},"page":"e0133558","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Establishment of a High-Throughput Assay to Monitor Influenza A Virus RNA Transcription and Replication"],"prefix":"10.1371","volume":"10","author":[{"given":"Zhen","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenlong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weilie","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhizhen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Cen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,7,21]]},"reference":[{"issue":"1416","key":"ref1","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1098\/rstb.2001.0979","article-title":"Plasmid-only rescue of influenza A virus vaccine candidates","volume":"356","author":"JH Schickli","year":"2001","journal-title":"Philosophical transactions of the Royal Society of London Series B, Biological sciences"},{"issue":"Suppl 1","key":"ref2","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1086\/591851","article-title":"Developing new antiviral agents for influenza treatment: what does the future hold?","volume":"48","author":"F Hayden","year":"2009","journal-title":"Clinical infectious diseases: an official publication of the Infectious Diseases Society of America"},{"issue":"5","key":"ref3","article-title":"Emergence of resistance to oseltamivir among influenza A(H1N1) viruses in Europe","volume":"13","author":"A Lackenby","year":"2008","journal-title":"Euro surveillance: bulletin europeen sur les maladies transmissibles = European communicable disease bulletin"},{"issue":"25","key":"ref4","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1056\/NEJMoa054512","article-title":"Oseltamivir resistance during treatment of influenza A (H5N1) infection","volume":"353","author":"MD de Jong","year":"2005","journal-title":"The New England journal of medicine"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1086\/518936","article-title":"Surveillance of resistance to adamantanes among influenza A(H3N2) and A(H1N1) viruses isolated worldwide","volume":"196","author":"VM Deyde","year":"2007","journal-title":"The Journal of infectious diseases"},{"issue":"4","key":"ref6","first-page":"153","article-title":"Influenza A: understanding the viral life cycle","volume":"82","author":"T Samji","year":"2009","journal-title":"The Yale journal of biology and medicine"},{"issue":"02","key":"ref7","doi-asserted-by":"crossref","first-page":"113","DOI":"10.4149\/av_2013_02_113","article-title":"The RNA polymerase of influenza A virus: mechanisms of viral transcription and replication","volume":"57","author":"E Fodor","year":"2013","journal-title":"Acta virologica"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.chom.2010.05.008","article-title":"Cellular networks involved in the influenza virus life cycle","volume":"7","author":"T Watanabe","year":"2010","journal-title":"Cell host & microbe"},{"issue":"7282","key":"ref9","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/nature08760","article-title":"Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication","volume":"463","author":"A Karlas","year":"2010","journal-title":"Nature"},{"issue":"7282","key":"ref10","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nature08699","article-title":"Human host factors required for influenza virus replication","volume":"463","author":"R Konig","year":"2010","journal-title":"Nature"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1021\/pr060432u","article-title":"Identification of cellular interaction partners of the influenza virus ribonucleoprotein complex and polymerase complex using proteomic-based approaches","volume":"6","author":"D Mayer","year":"2007","journal-title":"Journal of proteome research"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/rmv.575","article-title":"Host factors for replication and transcription of the influenza virus genome","volume":"18","author":"K Nagata","year":"2008","journal-title":"Reviews in medical virology"},{"issue":"45","key":"ref13","doi-asserted-by":"crossref","first-page":"19151","DOI":"10.1073\/pnas.1013592107","article-title":"High-throughput identification of compounds targeting influenza RNA-dependent RNA polymerase activity","volume":"107","author":"CY Su","year":"2010","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"8","key":"ref14","doi-asserted-by":"crossref","first-page":"e35234","DOI":"10.1371\/journal.pone.0035234","article-title":"Screen anti-influenza lead compounds that target the PA(C) subunit of H5N1 viral RNA polymerase","volume":"7","author":"L Li","year":"2012","journal-title":"PloS one"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.ymeth.2011.08.007","article-title":"Identification of influenza virus inhibitors which disrupt of viral polymerase protein-protein interactions","volume":"55","author":"G Chase","year":"2011","journal-title":"Methods"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/nbt.1638","article-title":"Identification of influenza A nucleoprotein as an antiviral target","volume":"28","author":"RY Kao","year":"2010","journal-title":"Nature biotechnology"},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/j.micinf.2012.02.012","article-title":"Identification of a novel compound with antiviral activity against influenza A virus depending on PA subunit of viral RNA polymerase","volume":"14","author":"K Yamada","year":"2012","journal-title":"Microbes and infection \/ Institut Pasteur"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"e1002668","DOI":"10.1371\/journal.ppat.1002668","article-title":"A novel small molecule inhibitor of influenza A viruses that targets polymerase function and indirectly induces interferon","volume":"8","author":"MB Ortigoza","year":"2012","journal-title":"PLoS pathogens"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1128\/AAC.00125-11","article-title":"Identification of BPR3P0128 as an inhibitor of cap-snatching activities of influenza virus","volume":"56","author":"JT Hsu","year":"2012","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"1\u20132","key":"ref20","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jviromet.2005.01.016","article-title":"Virus-inducible reporter genes as a tool for detecting and quantifying influenza A virus replication","volume":"126","author":"A Lutz","year":"2005","journal-title":"Journal of virological methods"},{"issue":"1\u20132","key":"ref21","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.virusres.2004.02.028","article-title":"Efficient generation and growth of influenza virus A\/PR\/8\/34 from eight cDNA fragments","volume":"103","author":"E de Wit","year":"2004","journal-title":"Virus research"},{"issue":"11","key":"ref22","doi-asserted-by":"crossref","first-page":"5669","DOI":"10.1128\/JVI.64.11.5669-5673.1990","article-title":"Determination of influenza virus proteins required for genome replication","volume":"64","author":"TS Huang","year":"1990","journal-title":"Journal of virology"},{"issue":"Pt 12","key":"ref23","doi-asserted-by":"crossref","first-page":"3689","DOI":"10.1099\/vir.0.80518-0","article-title":"Increased amounts of the influenza virus nucleoprotein do not promote higher levels of viral genome replication","volume":"85","author":"AE Mullin","year":"2004","journal-title":"The Journal of general virology"},{"issue":"17","key":"ref24","doi-asserted-by":"crossref","first-page":"9144","DOI":"10.1128\/JVI.78.17.9144-9153.2004","article-title":"The PA subunit is required for efficient nuclear accumulation of the PB1 subunit of the influenza A virus RNA polymerase complex","volume":"78","author":"E Fodor","year":"2004","journal-title":"Journal of virology"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0168-1702(89)90057-9","article-title":"Complementation and analysis of an NP mutant of influenza virus","volume":"12","author":"RA Li","year":"1989","journal-title":"Virus research"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1038\/srep01106","article-title":"A cell-based screening system for influenza A viral RNA transcription\/replication inhibitors","volume":"3","author":"M Ozawa","year":"2013","journal-title":"Scientific reports"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"e1001329","DOI":"10.1371\/journal.ppat.1001329","article-title":"Dissection of the influenza A virus endocytic routes reveals macropinocytosis as an alternative entry pathway","volume":"7","author":"E de Vries","year":"2011","journal-title":"PLoS pathogens"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1038\/nprot.2009.28","article-title":"Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo","volume":"4","author":"BA Tannous","year":"2009","journal-title":"Nature protocols"},{"issue":"6","key":"ref29","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1073\/pnas.0610977104","article-title":"Influenza virus targets the mRNA export machinery and the nuclear pore complex","volume":"104","author":"N Satterly","year":"2007","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"46","key":"ref30","doi-asserted-by":"crossref","first-page":"18235","DOI":"10.1073\/pnas.0705856104","article-title":"An influenza virus replicon system in yeast identified Tat-SF1 as a stimulatory host factor for viral RNA synthesis","volume":"104","author":"T Naito","year":"2007","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"24","key":"ref31","doi-asserted-by":"crossref","first-page":"12619","DOI":"10.1128\/JVI.01696-10","article-title":"Cyclin T1\/CDK9 interacts with influenza A virus polymerase and facilitates its association with cellular RNA polymerase II","volume":"84","author":"J Zhang","year":"2010","journal-title":"Journal of virology"},{"issue":"13","key":"ref32","doi-asserted-by":"crossref","first-page":"6197","DOI":"10.1128\/JVI.00277-11","article-title":"Replication-coupled and host factor-mediated encapsidation of the influenza virus genome by viral nucleoprotein","volume":"85","author":"A Kawaguchi","year":"2011","journal-title":"Journal of virology"},{"issue":"22","key":"ref33","doi-asserted-by":"crossref","first-page":"12062","DOI":"10.1128\/JVI.00684-11","article-title":"Cellular human CLE\/C14orf166 protein interacts with influenza virus polymerase and is required for viral replication","volume":"85","author":"A Rodriguez","year":"2011","journal-title":"Journal of virology"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1128\/JVI.00939-12","article-title":"Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation","volume":"87","author":"WB Hsu","year":"2013","journal-title":"Journal of virology"},{"issue":"4","key":"ref35","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00151-11","article-title":"Host- and strain-specific regulation of influenza virus polymerase activity by interacting cellular proteins","volume":"2","author":"E Bortz","year":"2011","journal-title":"mBio"},{"issue":"24","key":"ref36","doi-asserted-by":"crossref","first-page":"13010","DOI":"10.1128\/JVI.02651-10","article-title":"Generation and comprehensive analysis of an influenza virus polymerase cellular interaction network","volume":"85","author":"L Tafforeau","year":"2011","journal-title":"Journal of virology"},{"issue":"7","key":"ref37","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.cell.2009.12.018","article-title":"A physical and regulatory map of host-influenza interactions reveals pathways in H1N1 infection","volume":"139","author":"SD Shapira","year":"2009","journal-title":"Cell"},{"issue":"13","key":"ref38","doi-asserted-by":"crossref","first-page":"6429","DOI":"10.1128\/JVI.00293-09","article-title":"RuvB-like protein 2 is a suppressor of influenza A virus polymerases","volume":"83","author":"S Kakugawa","year":"2009","journal-title":"Journal of virology"},{"issue":"9","key":"ref39","doi-asserted-by":"crossref","first-page":"5585","DOI":"10.1128\/JVI.67.9.5585-5594.1993","article-title":"Ion channel activity of influenza A virus M2 protein: characterization of the amantadine block","volume":"67","author":"C Wang","year":"1993","journal-title":"Journal of virology"},{"issue":"14","key":"ref40","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1021\/jm980162u","article-title":"Structure-activity relationship studies of novel carbocyclic influenza neuraminidase inhibitors","volume":"41","author":"CU Kim","year":"1998","journal-title":"Journal of medicinal chemistry"},{"issue":"4050","key":"ref41","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1126\/science.177.4050.705","article-title":"Broad-spectrum antiviral activity of Virazole: 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide","volume":"177","author":"RW Sidwell","year":"1972","journal-title":"Science (New York, NY)"},{"issue":"4","key":"ref42","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1128\/AAC.46.4.977-981.2002","article-title":"In vitro and in vivo activities of anti-influenza virus compound T-705","volume":"46","author":"Y Furuta","year":"2002","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"2","key":"ref43","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays","volume":"4","author":"JH Zhang","year":"1999","journal-title":"J Biomol Screen"},{"issue":"3","key":"ref44","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1177\/1087057105285610","article-title":"A comparison of assay performance measures in screening assays: signal window, Z&apos; factor, and assay variability ratio","volume":"11","author":"PW Iversen","year":"2006","journal-title":"J Biomol Screen"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0133558","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,5]],"date-time":"2020-09-05T09:58:26Z","timestamp":1599299906000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0133558"}},"subtitle":[],"editor":[{"given":"Paul","family":"Digard","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,7,21]]},"references-count":44,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2015,7,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0133558","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,21]]}}}