{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T13:05:09Z","timestamp":1782997509446,"version":"3.54.5"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T00:00:00Z","timestamp":1439856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0135378","type":"journal-article","created":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T17:58:49Z","timestamp":1439920729000},"page":"e0135378","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["Flow Cytofluorimetric Analysis of Anti-LRP4 (LDL Receptor-Related Protein 4) Autoantibodies in Italian Patients with Myasthenia Gravis"],"prefix":"10.1371","volume":"10","author":[{"given":"Mariapaola","family":"Marino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Scuderi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Samengo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Saltelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Maiuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Sabatelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovambattista","family":"Pani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Antonini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia","family":"Evoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Bartoccioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,8,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/0090-1229(77)90027-7","article-title":"An assay for antibodies to human acetylcholine receptor in serum from patients with myasthenia gravis","volume":"7","author":"J Lindstrom","year":"1977","journal-title":"Clin Immunol Immunopathol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.coph.2012.02.002","article-title":"Signaling and aging at the neuromuscular synapse: lessons learnt from neuromuscular diseases","volume":"12","author":"AR Punga","year":"2012","journal-title":"Curr Opin Pharmacol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1093\/brain\/awn092","article-title":"IgG1 antibodies to acetylcholine receptors in 'seronegative' myasthenia gravis","volume":"131","author":"MI Leite","year":"2008","journal-title":"Brain"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jaut.2014.01.003","article-title":"Diagnostic and clinical classification of autoimmune myasthenia gravis","volume":"48\u201349","author":"S Berrih-Aknin","year":"2014","journal-title":"J Autoimmun"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/85520","article-title":"Auto-antibodies to the receptor tyrosine kinase MuSK in patients with myasthenia gravis without acetylcholine receptor antibodies","volume":"7","author":"W Hoch","year":"2001","journal-title":"Nat Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2304","DOI":"10.1093\/brain\/awg223","article-title":"Clinical correlates with anti-MuSK antibodies in generalized seronegative myasthenia gravis","volume":"126","author":"A Evoli","year":"2003","journal-title":"Brain"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/ana.20061","article-title":"Detection and characterization of MuSK antibodies in seronegative myasthenia gravis","volume":"55","author":"J McConville","year":"2004","journal-title":"Ann Neurol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/joa.12034","article-title":"The role of muscle-specific tyrosine kinase (MuSK) and mystery of MuSK myasthenia gravis","volume":"224","author":"I Koneczny","year":"2014","journal-title":"J Anat"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.neuron.2008.10.006","article-title":"LRP4 serves as a coreceptor of agrin","volume":"60","author":"B Zhang","year":"2008","journal-title":"Neuron"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"40624","DOI":"10.1074\/jbc.M111.279307","article-title":"Agrin binds to the N-terminal region of Lrp4 protein and stimulates association between Lrp4 and the first immunoglobulin-like domain in muscle-specific kinase (MuSK)","volume":"286","author":"W Zhang","year":"2011","journal-title":"J Biol Chem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"4993","DOI":"10.1242\/dev.02696","article-title":"LDL-receptor-related protein 4 is crucial for formation of the neuromuscular junction","volume":"133","author":"SD Weatherbee","year":"2006","journal-title":"Development"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1111\/j.1460-9568.2006.04846.x","article-title":"Interaction of LDL receptor-related protein 4 (LRP4) with postsynaptic scaffold proteins via its C-terminal PDZ domain-binding motif, and its regulation by Ca\/calmodulin-dependent protein kinase II","volume":"23","author":"QB Tian","year":"2006","journal-title":"Eur J Neurosci"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.neuron.2012.04.033","article-title":"Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation","volume":"75","author":"H Wu","year":"2012","journal-title":"Neuron"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"3077","DOI":"10.1007\/s00018-012-1209-9","article-title":"Structural mechanisms of the agrin-LRP4-MuSK signaling pathway in neuromuscular junction differentiation","volume":"70","author":"Y Zong","year":"2013","journal-title":"Cell Mol Life Sci"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1002\/ana.22312","article-title":"Autoantibodies to low-density lipoprotein receptor-related protein 4 in myasthenia gravis","volume":"69","author":"O Higuchi","year":"2011","journal-title":"Ann Neurol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1749-6632.2012.06833.x","article-title":"The search for new antigenic targets in myasthenia gravis","volume":"1275","author":"J Cossins","year":"2012","journal-title":"Ann N Y Acad Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s00415-011-6194-7","article-title":"Anti-LRP4 autoantibodies in AChR- and MuSK-antibody-negative myasthenia gravis","volume":"259","author":"A Pevzner","year":"2012","journal-title":"J Neurol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1001\/archneurol.2011.2393","article-title":"Autoantibodies to lipoprotein-related protein 4 in patients with double-seronegative myasthenia gravis","volume":"69","author":"B Zhang","year":"2012","journal-title":"Arch Neurol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1001\/archneurol.2011.2855","article-title":"Antibodies to low density lipoprotein receptor-related protein 4 in seronegative myasthenia gravis","volume":"69","author":"DP Richman","year":"2012","journal-title":"Arch Neurol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1002\/ana.21371","article-title":"Anti-MuSK patient antibodies disrupt the mouse neuromuscular junction","volume":"63","author":"RN Cole","year":"2008","journal-title":"Ann Neurol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e80695","DOI":"10.1371\/journal.pone.0080695","article-title":"MuSK myasthenia gravis IgG4 disrupts the interaction of LRP4 with MuSK but both IgG4 and IgG1-3 can disperse preformed agrin-independent AChR clusters","volume":"11","author":"I Koneczny","year":"2013","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1212\/WNL.0b013e318237f660","article-title":"Anti-MuSK autoantibodies block binding of collagen Q to MuSK","volume":"77","author":"Y Kawakami","year":"2011","journal-title":"Neurology"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.jneuroim.2009.11.004","article-title":"Anti-acetylcholinesterase antibodies associate with ocular myasthenia gravis","volume":"218","author":"C Provenzano","year":"2010","journal-title":"J Neuroimmunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"5190","DOI":"10.1172\/JCI66039","article-title":"Antibodies against low-density lipoprotein receptor-related protein 4 induce myasthenia gravis","volume":"123","author":"C Shen","year":"2013","journal-title":"J Clin Invest"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"13892","DOI":"10.1523\/JNEUROSCI.1733-14.2014","article-title":"LRP4 Is Critical for Neuromuscular Junction Maintenance","volume":"34","author":"A Barik","year":"2012","journal-title":"J Neurosci"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1212\/WNL.55.1.16","article-title":"Myasthenia gravis: recommendations for clinical research standards. Task force of the Medical Scientific Advisory Board of the Myasthenia Gravis Foundation of America","volume":"55","author":"A Jaretzki","year":"2000","journal-title":"Neurology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/S0165-5728(01)00287-9","article-title":"Constitutive and cytokine-induced expression of MHC and intercellular adhesion molecule-1 (ICAM-1) on human myoblasts","volume":"116","author":"M Marino","year":"2001","journal-title":"J Neuroimmunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0003-2697(84)90782-6","article-title":"A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids","volume":"138","author":"D Wessel","year":"1984","journal-title":"Anal Biochem"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jaut.2013.12.004","article-title":"A comprehensive analysis of the epidemiology and clinical characteristics of anti-LRP4 in myasthenia gravis","volume":"52","author":"P Zisimopoulou","year":"2014","journal-title":"J Autoimmun"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1016\/j.humimm.2013.06.020","article-title":"Autoimmune associations and autoantibody screening show focused recognition in patient subgroups with generalized myasthenia gravis","volume":"74","author":"R Klein","year":"2013","journal-title":"Hum Immunol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1212\/01.WNL.0000150587.71497.B6","article-title":"Thymus changes in anti-MuSK-positive and -negative myasthenia gravis","volume":"64","author":"L Lauriola","year":"2005","journal-title":"Neurology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e91816","DOI":"10.1371\/journal.pone.0091816","article-title":"Autoantibodies to agrin in myasthenia gravis patients","volume":"9","author":"B Zhang","year":"2014","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1212\/WNL.0000000000000478","article-title":"Anti-agrin autoantibodies in myasthenia gravis","volume":"82","author":"C Gasperi","year":"2014","journal-title":"Neurology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.autrev.2014.08.039","article-title":"Cortactin autoantibodies in myasthenia gravis","volume":"13","author":"E Gallardo","year":"2014","journal-title":"Autoimmun Rev"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/acn3.26","article-title":"LRP4 antibodies in serum and CSF from amyotrophic lateral sclerosis patients","volume":"1","author":"JS Tzartos","year":"2014","journal-title":"Ann Clin Transl Neurol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0135378","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T10:02:17Z","timestamp":1540029737000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0135378"}},"subtitle":[],"editor":[{"given":"G\u00fcher","family":"Saruhan-Direskeneli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,8,18]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2015,8,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0135378","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,18]]}}}