{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T01:17:54Z","timestamp":1768699074083,"version":"3.49.0"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2015,8,28]],"date-time":"2015-08-28T00:00:00Z","timestamp":1440720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0136865","type":"journal-article","created":{"date-parts":[[2015,8,28]],"date-time":"2015-08-28T18:48:12Z","timestamp":1440787692000},"page":"e0136865","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (\u0394abcBA) Protects against Wild Type Brucella ovis in Rams"],"prefix":"10.1371","volume":"10","author":[{"given":"Ana Patr\u00edcia C.","family":"Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Auric\u00e9lio A.","family":"Mac\u00eado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana F.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia E.","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luize N. N.","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jade R. D.","family":"Farias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscilla P. R.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo C.","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kessler W. J.","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a R. F.","family":"Maia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela G.","family":"Neves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Everton L.","family":"Rom\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teane M. A.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana P. S.","family":"Mol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata M.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio S. S.","family":"Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernane F.","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olindo A.","family":"Martins-Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto M.","family":"Brand\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiane A.","family":"Paix\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato L.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,8,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"MP Franco","year":"2007","journal-title":"Lancet Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1590\/S0100-736X2013000600012","article-title":"Economic losses due to bovine brucellosis in Brazil","volume":"33","author":"RL Santos","year":"2013","journal-title":"Pesq Vet Bras"},{"key":"ref3","article-title":"Bergey\u2019s Manual of Systematic Bacteriology","author":"GM Garrity","year":"2001"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"105","DOI":"10.20506\/rst.32.1.2193","article-title":"Pathogenesis and pathobiology of brucellosis in livestock","volume":"32","author":"FP Poester","year":"2013","journal-title":"Rev Sci Tech"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.smallrumres.2011.08.004","article-title":"Andrological, pathologic, morphometric, and ultrasonographic findings in rams experimentally infected with <italic>Brucella ovis<\/italic>","volume":"102","author":"CA Carvalho Junior","year":"2012","journal-title":"Small Rumin Res"},{"key":"ref6","first-page":"351","article-title":"<italic>Animal Brucellosis<\/italic>","author":"JM Blasco","year":"1990"},{"key":"ref7","first-page":"236","article-title":"Pathogenesis of bacterial infections in animals","author":"CO Thoen","year":"1993"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1297-9716-45-61","article-title":"Evaluation in mice of <italic>Brucella ovis<\/italic> attenuated mutants for use as live vaccines against <italic>B<\/italic>. <italic>ovis<\/italic>","volume":"45","author":"P Sancho","year":"2014","journal-title":"Vet Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13567-014-0072-0","article-title":"Mutants in the lipopolysaccharide of <italic>Brucella ovis<\/italic> are attenuated and protect against <italic>B<\/italic>. <italic>ovis<\/italic> infection in mice","volume":"45","author":"P Soler-Llor\u00e9ns","year":"2014","journal-title":"Vet Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"6704","DOI":"10.1016\/j.vaccine.2009.08.097","article-title":"The polymeric antigen BLSOmp31 confers protection against <italic>Brucella ovis<\/italic> infection in rams","volume":"27","author":"SM Estein","year":"2009","journal-title":"Vaccine"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"4437","DOI":"10.1016\/j.vaccine.2007.03.028","article-title":"A recombinat subunit vaccine based on the insertion of 27 aminoacids from Omp31 to the N-terminous of BLS induced a similar degree of protection against <italic>B<\/italic>. <italic>ovis<\/italic> than Rev 1 vaccination","volume":"25","author":"J Cassataro","year":"2007","journal-title":"Vaccine"},{"key":"ref12","article-title":"Brucellosis in humans and animals","author":"MJ Corbel","year":"2006"},{"key":"ref13","first-page":"743509","article-title":"A History of the development of <italic>Brucella<\/italic> vaccines","volume":"2013","author":"ED Avila-Calder\u00f3n","year":"2013","journal-title":"BioMed Res Int"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"207","DOI":"10.20506\/rst.32.1.2201","article-title":"Recent developments in livestock and wildlife brucellosis vaccination","volume":"32","author":"SC Olsen","year":"2013","journal-title":"Rev Sci Tech"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0034-5288(18)30992-5","article-title":"Comparison of subcutaneous and conjunctival routes of Rev 1 vaccination for the prophylaxis of <italic>Brucella ovis<\/italic> infection in rams","volume":"48","author":"CM Mar\u00edn","year":"1990","journal-title":"Res Vet Sci"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0167-5877(96)01110-5","article-title":"A review of the use of <italic>B<\/italic>. <italic>melitensis<\/italic> Rev 1 vaccine in adult sheep and goats","volume":"31","author":"JM Blasco","year":"1997","journal-title":"Prev Vet Med"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1006\/biol.2000.0249","article-title":"In vitro markers and biological activity in mice of seed lot strains and commercial <italic>Brucella melitensis<\/italic> Rev.1 and <italic>Brucella abortus<\/italic> B19 vaccines","volume":"28","author":"MJ Grill\u00f3","year":"2000","journal-title":"Biologicals"},{"key":"ref18","first-page":"247","article-title":"<italic>Brucella melitensis<\/italic>","author":"F Lantier","year":"1985"},{"key":"ref19","first-page":"2003","article-title":"Guidelines for coordinated human and animal brucellosis surveillance In FAO ANIMAL PRODUCTION AND HEALTH PAPER","volume":"156","author":"A Robinson","journal-title":"Guidelines for coordinated human and animal brucellosis surveillance In FAO ANIMAL PRODUCTION AND HEALTH PAPER"},{"key":"ref20","first-page":"85","article-title":"The use of live vaccine for vaccination of human beings against brucellosis in the USSR","volume":"24","author":"PA Vershilova","year":"1961","journal-title":"Bull World Health Organ"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0378-1135(02)00233-X","article-title":"<italic>Brucella<\/italic> vaccines in wildlife","volume":"90","author":"DS Davis","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, presentand future","volume":"90","author":"GG Schurig","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1128\/IAI.01109-10","article-title":"Putative ATP-binding cassette transporter is essential for <italic>Brucella ovis<\/italic> pathogenesis in mice","volume":"79","author":"TMA Silva","year":"2011","journal-title":"Infect Immun"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1016\/j.vetmic.2013.09.003","article-title":"<italic>Brucella ovis<\/italic> lacking a species-specific putative ATP-binding cassete transporter is attenuated but immunogenic in rams","volume":"167","author":"APC Silva","year":"2013","journal-title":"Vet Microbiol"},{"key":"ref25","article-title":"Protection of an encapsulated live attenuated strain of <italic>Brucella ovis<\/italic> against experimental challenge in the murine model","author":"APC Silva","year":"2015","journal-title":"Clin Vaccine Immunol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e114532","DOI":"10.1371\/journal.pone.0114532","article-title":"The predicted ABC transporter AbcEDCBA is required for type IV secretion system expression and lysosomal evasion by <italic>Brucella ovis<\/italic>","volume":"12","author":"TMA Silva","year":"2014","journal-title":"Plos One"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.vetmic.2010.02.037","article-title":"Development and evaluation of a species-specific PCR assay for the detection of <italic>Brucella ovis<\/italic> infection in rams","volume":"145","author":"MN Xavier","year":"2010","journal-title":"Vet Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1590\/S1517-83822009000300010","article-title":"Evaluation of DNA extraction protocols for <italic>Brucella abortus<\/italic> pcr detection in aborted fetuses or calves born from cows experimentally infected with strain 2308","volume":"40","author":"M Matrone","year":"2009","journal-title":"Braz J Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.vetmic.2012.03.029","article-title":"The <italic>virB<\/italic> encode type IV secretion system is critical for establishment of infection and persistence of <italic>Brucella ovis<\/italic> infection in mice","volume":"159","author":"JC S\u00e1","year":"2012","journal-title":"Vet Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0378-1135(02)00231-6","article-title":"Control of small ruminant brucellosis by use of <italic>Brucella melitensis<\/italic> Rev. 1 vaccine: laboratory aspects and field observations","volume":"90","author":"M Banai","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0378-1135(02)00229-8","article-title":"Diagnosis of brucellosis by serology","volume":"90","author":"K Nielsen","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0169-409X(97)00053-7","article-title":"New advances in microsphere based single-dose vaccines","volume":"28","author":"J Hanes","year":"1997","journal-title":"Adv Drug Del Rev"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/S1389-1723(00)87103-0","article-title":"Formation of calcium alginate gel capsules: influence of sodium alginate and CaCl<sub>2<\/sub> concentration on gelation kinetics","volume":"88","author":"A Blandino","year":"1999","journal-title":"J Biosci Bioeng"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1016\/S0142-9612(00)00039-9","article-title":"Oral peptide drug delivery: polymer-inhibitor conjugates protecting insulin from enzymatic degradation <italic>in vitro<\/italic>","volume":"21","author":"MK Marschutz","year":"2000","journal-title":"Biomaterials"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0168-3659(01)00518-1","article-title":"A novel sustained-release formulation of insulin with dramatic reduction in initial rapid release","volume":"79","author":"M Takenaga","year":"2002","journal-title":"J Control Release"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0142-9612(02)00227-2","article-title":"A hydrogel prepared by in situ cross-linking of a thiol-containing poly(ethylene glycol)-based copolymer: a new biomaterial for protein drug delivery","volume":"24","author":"B Qiu","year":"2003","journal-title":"Biomaterials"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1177\/088532828700200103","article-title":"Tissue response to biodegradable injectable microcapsules","volume":"2","author":"GE Visscher","year":"1987","journal-title":"J Biomater Appl"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"4099","DOI":"10.1016\/S0264-410X(01)00177-3","article-title":"A <italic>Brucella ovis<\/italic> antigenic complex bearing poly-epsilon-caprolactone microparticles confer protection against experimental brucellosis in mice","volume":"19","author":"M Murillo","year":"2001","journal-title":"Vaccine"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.1128\/IAI.00767-07","article-title":"Immunization with a single dose of a microencapsulated <italic>Brucella melitensis<\/italic> mutant enhances protection against wild-type challenge","volume":"76","author":"AM Arenas-Gamboa","year":"2008","journal-title":"Infect Immun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"165","DOI":"10.7589\/0090-3558-45.1.165","article-title":"Enhanced immune response of red deer (<italic>Cervus elaphus<\/italic>) to live RB51 vaccine strain using composite microspheres","volume":"45","author":"AM Arenas-Gamboa","year":"2009","journal-title":"J Wild Dis"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1128\/IAI.01017-08","article-title":"The <italic>Brucella abortus<\/italic> S19 \u0394<italic>vjbR<\/italic> live candidate is safer than S19 and confers protection against wild-type vaccine challenge in BALB\/c mice when delivered in a sustained-release vehicle","volume":"77","author":"AM Arenas-Gamboa","year":"2009","journal-title":"Infect Immun"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"241452","DOI":"10.1155\/2014\/241452","article-title":"Clincal and pathological changes in rams experimentally infected with <italic>Actinobacillus seminis<\/italic> and <italic>Histophilus somni<\/italic>","volume":"2014","author":"VS Moustacas","year":"2014","journal-title":"Sci World J"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1590\/S0102-09352012000300029","article-title":"Diagnosis of Brucella ovis infection by serology and PCR in urine samples from naturally infected rams in the state of Piau\u00ed","volume":"64","author":"EA Costa","year":"2012","journal-title":"Arq Bras Med Vet Zootec"},{"key":"ref44","first-page":"263","article-title":"The pathology of bacterial infection of the genitalia in rams","volume":"47","author":"BC Jansen","year":"1980","journal-title":"J Vet Res"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0136865","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T20:29:28Z","timestamp":1588969768000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0136865"}},"subtitle":[],"editor":[{"given":"Axel","family":"Cloeckaert","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,8,28]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2015,8,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0136865","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,28]]}}}