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Xpert MTB\/RIF implementation manual: Technical and operational &apos;how to&apos; practical considerations. 2014; Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/apps.who.int\/iris\/bitstream\/10665\/112469\/1\/9789241506700_eng.pdf\" xlink:type=\"simple\">http:\/\/apps.who.int\/iris\/bitstream\/10665\/112469\/1\/9789241506700_eng.pdf<\/ext-link>. 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Western Cape Provincial Department of Health circular 2014 September 16 2014; Circular number H157\/2014(H157):1\u20135."},{"issue":"10","key":"ref14","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1093\/cid\/cis190","article-title":"Increased and expedited case detection by Xpert MTB\/RIF assay in childhood tuberculosis: a prospective cohort study","volume":"54","author":"A Rachow","year":"2012","journal-title":"Clin Infect Dis"},{"issue":"11","key":"ref15","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/S1473-3099(11)70167-0","article-title":"Accuracy of the Xpert MTB\/RIF test for the diagnosis of pulmonary tuberculosis in children admitted to hospital in Cape Town, South Africa: a descriptive study","volume":"11","author":"MP Nicol","year":"2011","journal-title":"The Lancet infectious diseases"},{"issue":"9776","key":"ref16","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/S0140-6736(11)60438-8","article-title":"Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB\/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study","volume":"377","author":"CC Boehme","year":"2011","journal-title":"The lancet"},{"issue":"11","key":"ref17","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1056\/NEJMoa0907847","article-title":"Rapid molecular detection of tuberculosis and rifampin resistance","volume":"363","author":"CC Boehme","year":"2010","journal-title":"N Engl J Med"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1164\/ajrccm.161.4.16141","article-title":"Diagnostic standards and classification of tuberculosis in adults and children","volume":"161","year":"2000","journal-title":"Am J Respir Crit Care Med"},{"issue":"6","key":"ref19","first-page":"998","article-title":"Quantitative studies of mycobacterial populations in sputum and saliva","volume":"95","author":"H Yeager Jr","year":"1967","journal-title":"Am Rev Respir Dis"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1128\/AAC.4.2.94","article-title":"Enumeration of tubercle bacilli in sputum of patients with pulmonary tuberculosis","volume":"4","author":"GL Hobby","year":"1973","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"ref21","doi-asserted-by":"crossref","first-page":"e1001536","DOI":"10.1371\/journal.pmed.1001536","article-title":"Diagnostic accuracy of quantitative PCR (Xpert MTB\/RIF) for tuberculous meningitis in a high burden setting: a prospective study","volume":"10","author":"VB Patel","year":"2013","journal-title":"PLoS medicine"},{"issue":"9","key":"ref22","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1093\/cid\/cis631","article-title":"Evaluation of the Xpert MTB\/RIF assay at a tertiary care referral hospital in a setting where tuberculosis and HIV infection are highly endemic","volume":"55","author":"J O'Grady","year":"2012","journal-title":"Clin Infect Dis"},{"issue":"Suppl 2","key":"ref23","doi-asserted-by":"crossref","first-page":"S340","DOI":"10.1093\/infdis\/jir859","article-title":"Tuberculosis and tuberculosis\/HIV\/AIDS-associated mortality in Africa: the urgent need to expand and invest in routine and research autopsies","volume":"205","author":"V Mudenda","year":"2012","journal-title":"J Infect Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"207","DOI":"10.2307\/2531961","article-title":"Sample size for testing differences in proportions for the paired-sample design","author":"RJ Connor","year":"1987","journal-title":"Biometrics"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0141623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T14:23:58Z","timestamp":1589034238000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0141623"}},"subtitle":[],"editor":[{"given":"Sarah L","family":"Pett","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,11,10]]},"references-count":24,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2015,11,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0141623","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,10]]}}}