{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:49:59Z","timestamp":1789627799580,"version":"build-2803163510"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2015,11,10]],"date-time":"2015-11-10T00:00:00Z","timestamp":1447113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0142219","type":"journal-article","created":{"date-parts":[[2015,11,10]],"date-time":"2015-11-10T19:18:23Z","timestamp":1447183103000},"page":"e0142219","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["SHP-2 Mediates Cryptosporidium parvum Infectivity in Human Intestinal Epithelial Cells"],"prefix":"10.1371","volume":"10","author":[{"given":"Eunice A.","family":"Varughese","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Kasper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily M.","family":"Anneken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagjit S.","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,11,10]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.pt.2013.03.001","article-title":"Looking for Cryptosporidium: the application of advances in detection and diagnosis","volume":"29","author":"RM Chalmers","year":"2013","journal-title":"Trends in Parasitology"},{"key":"ref2","article-title":"Research Priorities for Zoonoses and Marginalized Infections","year":"2012"},{"issue":"48","key":"ref3","doi-asserted-by":"crossref","first-page":"34877","DOI":"10.1074\/jbc.M706950200","article-title":"Cryptosporidium p30, a galactose\/N-acetylgalactosamine-specific lectin, mediates infection in vitro","volume":"282","author":"N Bhat","year":"2007","journal-title":"The Journal of biological chemistry"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/pim.12115","article-title":"Apicomplexan infections in the gut. Parasite immunology","volume":"36","author":"CL Wilhelm","year":"2014","journal-title":"Apicomplexan infections in the gut. Parasite immunology"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/S0016-5085(03)00662-0","article-title":"<italic>Cryptosporidium parvum<\/italic> invasion of biliary epithelia requires host cell tyrosine phosphorylation of cortactin via c-Src","volume":"125","author":"XM Chen","year":"2003","journal-title":"Gastroenterology"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"3011","DOI":"10.1128\/IAI.72.5.3011-3021.2004","article-title":"Cdc42 and the actin-related protein\/neural Wiskott-Aldrich syndrome protein network mediate cellular invasion by <italic>Cryptosporidium parvum<\/italic>","volume":"72","author":"XM Chen","year":"2004","journal-title":"Infection and immunity"},{"issue":"30","key":"ref7","doi-asserted-by":"crossref","first-page":"31671","DOI":"10.1074\/jbc.M401592200","article-title":"Phosphatidylinositol 3-kinase and frabin mediate <italic>Cryptosporidium parvum<\/italic> cellular invasion via activation of Cdc42","volume":"279","author":"XM Chen","year":"2004","journal-title":"The Journal of biological chemistry"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1128\/IAI.67.2.844-852.1999","article-title":"A role for host phosphoinositide 3-kinase and cytoskeletal remodeling during <italic>Cryptosporidium parvum<\/italic> infection","volume":"67","author":"JR Forney","year":"1999","journal-title":"Infection and immunity"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1128\/IAI.05862-11","article-title":"Involvement of host cell integrin alpha2 in <italic>Cryptosporidium parvum<\/italic> infection","volume":"80","author":"H Zhang","year":"2012","journal-title":"Infection and immunity"},{"key":"ref10","article-title":"Functions of Shp2 in cancer","author":"J Zhang","year":"2015","journal-title":"Journal of cellular and molecular medicine"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"e0123472","DOI":"10.1371\/journal.pone.0123472","article-title":"The Tyrosine Phosphatase SHP2 Associates with CUB Domain-Containing Protein-1 (CDCP1), Regulating Its Expression at the Cell Surface in a Phosphorylation-Dependent Manner","volume":"10","author":"LY Gandji","year":"2015","journal-title":"PloS one"},{"issue":"7","key":"ref12","doi-asserted-by":"crossref","first-page":"e102847","DOI":"10.1371\/journal.pone.0102847","article-title":"Tyrosine phosphatase Shp2 mediates the estrogen biological action in breast cancer via interaction with the estrogen extranuclear receptor","volume":"9","author":"J Li","year":"2014","journal-title":"PloS one"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1006\/excr.1999.4668","article-title":"Shp-2 tyrosine phosphatase: signaling one cell or many","volume":"253","author":"GS Feng","year":"1999","journal-title":"Experimental cell research"},{"issue":"6","key":"ref14","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/S0968-0004(03)00091-4","article-title":"The &apos;Shp&apos;ing news: SH2 domain-containing tyrosine phosphatases in cell signaling","volume":"28","author":"BG Neel","year":"2003","journal-title":"Trends in biochemical sciences"},{"issue":"9","key":"ref15","doi-asserted-by":"crossref","first-page":"4316","DOI":"10.1091\/mbc.E05-02-0131","article-title":"Src and FAK kinases cooperate to phosphorylate paxillin kinase linker, stimulate its focal adhesion localization, and regulate cell spreading and protrusiveness","volume":"16","author":"MC Brown","year":"2005","journal-title":"Molecular biology of the cell"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/sj.onc.1203204","article-title":"Roles for the protein tyrosine phosphatase SHP-2 in cytoskeletal organization, cell adhesion and cell migration revealed by overexpression of a dominant negative mutant","volume":"19","author":"K Inagaki","year":"2000","journal-title":"Oncogene"},{"issue":"19","key":"ref17","doi-asserted-by":"crossref","first-page":"5023","DOI":"10.1093\/emboj\/cdg492","article-title":"Force-dependent integrin-cytoskeleton linkage formation requires downregulation of focal complex dynamics by Shp2","volume":"22","author":"G von Wichert","year":"2003","journal-title":"The EMBO journal"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1128\/MCB.19.4.3125","article-title":"Concerted activity of tyrosine phosphatase SHP-2 and focal adhesion kinase in regulation of cell motility","volume":"19","author":"S Manes","year":"1999","journal-title":"Molecular and cellular biology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mimet.2014.07.017","article-title":"A new in vitro model using small intestinal epithelial cells to enhance infection of Cryptosporidium parvum","volume":"106","author":"EA Varughese","year":"2014","journal-title":"Journal of Microbiological Methods"},{"issue":"3\u20134","key":"ref20","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.vetpar.2009.11.019","article-title":"Improved Cryptosporidium parvum oocyst propagation using dexamethasone suppressed CF-1 mice","volume":"168","author":"MW Ware","year":"2010","journal-title":"Veterinary Parasitology"},{"issue":"49","key":"ref21","doi-asserted-by":"crossref","first-page":"6365","DOI":"10.1038\/onc.2008.250","article-title":"Src family kinase oncogenic potential and pathways in prostate cancer as revealed by AZD0530","volume":"27","author":"YM Chang","year":"2008","journal-title":"Oncogene"},{"issue":"5","key":"ref22","first-page":"56S","article-title":"<italic>Cryptosporidium parvum<\/italic>: synchronized excystation in vitro and evaluation of sporozoite infectivity with a new lectin-based assay","volume":"46","author":"J Gut","year":"1999","journal-title":"Journal of Eukaryotic Microbiology"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1152\/physrev.00002.2004","article-title":"Paxillin: adapting to change","volume":"84","author":"MC Brown","year":"2004","journal-title":"Physiological reviews"},{"issue":"Pt 15","key":"ref24","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1242\/jcs.018044","article-title":"Paxillin comes of age","volume":"121","author":"NO Deakin","year":"2008","journal-title":"Journal of cell science"},{"issue":"44","key":"ref25","doi-asserted-by":"crossref","first-page":"6459","DOI":"10.1038\/sj.onc.1204786","article-title":"Paxillin: a focal adhesion-associated adaptor protein","volume":"20","author":"MD Schaller","year":"2001","journal-title":"Oncogene"},{"issue":"9","key":"ref26","doi-asserted-by":"crossref","first-page":"8497","DOI":"10.1074\/jbc.M312575200","article-title":"Roles of Gab1 and SHP2 in paxillin tyrosine dephosphorylation and Src activation in response to epidermal growth factor","volume":"279","author":"Y Ren","year":"2004","journal-title":"The Journal of biological chemistry"},{"issue":"8\u20139","key":"ref27","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/j.ijpara.2007.11.003","article-title":"Cryptosporidia: epicellular parasites embraced by the host cell membrane","volume":"38","author":"A Valigurova","year":"2008","journal-title":"International Journal of Parasitology"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1128\/IAI.68.4.2315-2322.2000","article-title":"<italic>Cryptosporidium parvum<\/italic> induces host cell actin accumulation at the host-parasite interface","volume":"68","author":"DA Elliott","year":"2000","journal-title":"Infection and immunity"},{"issue":"5555","key":"ref29","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1126\/science.1067147","article-title":"SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein","volume":"295","author":"H Higashi","year":"2002","journal-title":"Science"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/978-0-387-78267-6_2","article-title":"Microneme proteins in apicomplexans","volume":"47","author":"VB Carruthers","year":"2008","journal-title":"Sub-cellular biochemistry"},{"issue":"4\u20135","key":"ref31","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.ijmm.2012.07.012","article-title":"Does protein phosphorylation govern host cell entry and egress by the Apicomplexa?","volume":"302","author":"D Jacot","year":"2012","journal-title":"International journal of medical microbiology: IJMM"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.pt.2005.01.007","article-title":"Cryptosporidium excystation and invasion: getting to the guts of the matter","volume":"21","author":"HV Smith","year":"2005","journal-title":"Trends in Parasitology"},{"issue":"2","key":"ref33","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.virol.2009.11.030","article-title":"The phosphatase Shp2 is required for signaling by the Kaposi&apos;s sarcoma-associated herpesvirus viral GPCR in primary endothelial cells","volume":"397","author":"T Bakken","year":"2010","journal-title":"Virology"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1177\/0883073807309247","article-title":"Increased expression of tyrosine phosphatase SHP2 in experimental pneumococcal meningitis: correlation with tumor necrosis factor-alpha and cerebrospinal fluid pleocytosis","volume":"23","author":"M Feng","year":"2008","journal-title":"Journal of child neurology"},{"issue":"4","key":"ref35","doi-asserted-by":"crossref","first-page":"575","DOI":"10.3748\/wjg.v19.i4.575","article-title":"Increased expression of tyrosine phosphatase SHP-2 in Helicobacter pylori-infected gastric cancer","volume":"19","author":"J Jiang","year":"2013","journal-title":"World journal of gastroenterology: WJG"},{"issue":"9","key":"ref36","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1016\/j.cellsig.2013.05.020","article-title":"Enteropathogenic Escherichia coli Tir recruits cellular SHP-2 through ITIM motifs to suppress host immune response","volume":"25","author":"D Yan","year":"2013","journal-title":"Cellular signalling"},{"issue":"17","key":"ref37","doi-asserted-by":"crossref","first-page":"17205","DOI":"10.1074\/jbc.M309964200","article-title":"Helicobacter pylori CagA induces Ras-independent morphogenetic response through SHP-2 recruitment and activation","volume":"279","author":"H Higashi","year":"2004","journal-title":"The Journal of biological chemistry"},{"issue":"6","key":"ref38","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.chom.2012.04.013","article-title":"The Helicobacter pylori virulence effector CagA abrogates human beta-defensin 3 expression via inactivation of EGFR signaling","volume":"11","author":"B Bauer","year":"2012","journal-title":"Cell host & microbe"},{"issue":"2","key":"ref39","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1128\/JVI.01362-10","article-title":"The Kaposi&apos;s sarcoma-associated herpesvirus G protein-coupled receptor contains an immunoreceptor tyrosine-based inhibitory motif that activates Shp2","volume":"85","author":"N Philpott","year":"2011","journal-title":"Journal of virology"},{"issue":"4","key":"ref40","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1083\/jcb.200202117","article-title":"Localized suppression of RhoA activity by Tyr31\/118-phosphorylated paxillin in cell adhesion and migration","volume":"159","author":"A Tsubouchi","year":"2002","journal-title":"The Journal of cell biology"},{"issue":"21","key":"ref41","doi-asserted-by":"crossref","first-page":"13223","DOI":"10.1074\/jbc.273.21.13223","article-title":"Integrin-mediated tyrosine phosphorylation of SHPS-1 and its association with SHP-2. Roles of Fak and Src family kinases","volume":"273","author":"M Tsuda","year":"1998","journal-title":"The Journal of biological chemistry"},{"issue":"11","key":"ref42","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1038\/sj.onc.1202513","article-title":"The Shp-2 tyrosine phosphatase activates the Src tyrosine kinase by a non-enzymatic mechanism","volume":"18","author":"AO Walter","year":"1999","journal-title":"Oncogene"},{"issue":"1","key":"ref43","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4161\/viru.1.1.10486","article-title":"Reinforcement of epithelial cell adhesion to basement membrane by a bacterial pathogen as a new infectious stratagem","volume":"1","author":"M Kim","year":"2010","journal-title":"Virulence"},{"issue":"1","key":"ref44","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/nrm1549","article-title":"Focal adhesion kinase: in command and control of cell motility","volume":"6","author":"SK Mitra","year":"2005","journal-title":"Nature reviews Molecular cell biology"},{"issue":"2","key":"ref45","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/j.1742-4658.2012.08655.x","article-title":"Protein tyrosine phosphatase structure-function relationships in regulation and pathogenesis","volume":"280","author":"F Bohmer","year":"2013","journal-title":"The FEBS journal"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0142219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T05:45:43Z","timestamp":1599803143000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0142219"}},"subtitle":[],"editor":[{"given":"Utpal","family":"Pal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,11,10]]},"references-count":45,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2015,11,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0142219","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,10]]}}}