{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T07:55:07Z","timestamp":1771919707153,"version":"3.50.1"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T00:00:00Z","timestamp":1450656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0145116","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T18:38:16Z","timestamp":1450723096000},"page":"e0145116","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of miRNAs Involved in Reprogramming Acinar Cells into Insulin Producing Cells"],"prefix":"10.1371","volume":"10","author":[{"given":"Joan","family":"Teichenne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meritxell","family":"Morr\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba","family":"Casellas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronica","family":"Jimenez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noelia","family":"Tellez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"Leger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Bosch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduard","family":"Ayuso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2015,12,21]]},"reference":[{"key":"ref1","unstructured":"Diabetes Atlas. In: International Diabetes Federation [Internet]. [cited 27 Jan 2015]. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.idf.org\/diabetesatlas\" xlink:type=\"simple\">http:\/\/www.idf.org\/diabetesatlas<\/ext-link>."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/gt.2013.50","article-title":"Stable insulin-secreting ducts formed by reprogramming of cells in the liver using a three-gene cocktail and a PPAR agonist","volume":"21","author":"A Banga","year":"2014","journal-title":"Gene Ther"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"15336","DOI":"10.1073\/pnas.1201701109","article-title":"In vivo reprogramming of Sox9+ cells in the liver to insulin-secreting ducts","volume":"109","author":"A Banga","year":"2012","journal-title":"Proc Natl Acad Sci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1038\/75050","article-title":"Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia","volume":"6","author":"S Ferber","year":"2000","journal-title":"Nat Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.2337\/diabetes.54.4.1009","article-title":"PDX-1\/VP16 fusion protein, together with NeuroD or Ngn3, markedly induces insulin gene transcription and ameliorates glucose tolerance","volume":"54","author":"H Kaneto","year":"2005","journal-title":"Diabetes"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s11373-008-9233-3","article-title":"Adeno-associated virus-mediated pancreatic and duodenal homeobox gene-1 expression enhanced differentiation of hepatic oval stem cells to insulin-producing cells in diabetic rats","volume":"15","author":"H Li","year":"2008","journal-title":"J Biomed Sci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/sj.mt.6300032","article-title":"Adenovirus transduction is required for the correction of diabetes using Pdx-1 or Neurogenin-3 in the liver","volume":"15","author":"AY Wang","year":"2007","journal-title":"Mol Ther J Am Soc Gene Ther"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1038\/nbt.3082","article-title":"Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells","volume":"32","author":"W Li","year":"2014","journal-title":"Nat Biotechnol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1038\/nature07314","article-title":"In vivo reprogramming of adult pancreatic exocrine cells to \u03b2-cells","volume":"455","author":"Q Zhou","year":"2008","journal-title":"Nature"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0304-3835(79)80080-4","article-title":"Transplantation of azaserine-induced carcinomas of pancreas in rats","volume":"7","author":"DS Longnecker","year":"1979","journal-title":"Cancer Lett"},{"key":"ref11","first-page":"G963","article-title":"Pancreatic tumoral cell line AR42J: an amphicrine model","volume":"266","author":"J Christophe","year":"1994","journal-title":"Am J Physiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1172\/JCI118591","article-title":"Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells","volume":"97","author":"H Mashima","year":"1996","journal-title":"J Clin Invest"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1042\/BJ20111678","article-title":"Reprogramming of pancreatic exocrine cells towards a beta (\u03b2) cell character using Pdx1, Ngn3 and MafA","volume":"442","author":"E Akinci","year":"2012","journal-title":"Biochem J"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.lfs.2014.08.001","article-title":"Expression of biologically active TAT-fused recombinant islet transcription factors","volume":"114","author":"MS Lan","year":"2014","journal-title":"Life Sci"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.mce.2012.02.024","article-title":"Efficient differentiation of AR42J cells towards insulin-producing cells using pancreatic transcription factors in combination with growth factors","volume":"358","author":"MJ Lima","year":"2012","journal-title":"Mol Cell Endocrinol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1210\/me.2007-0028","article-title":"MafA regulates expression of genes important to islet beta-cell function","volume":"21","author":"T Matsuoka","year":"2007","journal-title":"Mol Endocrinol Baltim Md"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1507\/endocrj.K07E-169","article-title":"Combined expression of transcription factors induces AR42J-B13 cells to differentiate into insulin-producing cells","volume":"55","author":"T Ogihara","year":"2008","journal-title":"Endocr J"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.2337\/diabetes.50.2007.S10","article-title":"Changes in the expression of transcription factors in pancreatic AR42J cells during differentiation into insulin-producing cells","volume":"50","author":"Y-Q Zhang","year":"2001","journal-title":"Diabetes"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.2337\/db07-0175","article-title":"MicroRNA expression is required for pancreatic islet cell genesis in the mouse","volume":"56","author":"FC Lynn","year":"2007","journal-title":"Diabetes"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/j.bbamcr.2010.06.013","article-title":"MicroRNAs: synthesis, mechanism, function, and recent clinical trials","volume":"1803","author":"F Wahid","year":"2010","journal-title":"Biochim Biophys Acta"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1161\/CIRCRESAHA.112.269035","article-title":"MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fibroblasts to cardiomyocytes","volume":"110","author":"TM Jayawardena","year":"2012","journal-title":"Circ Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.bbrc.2014.09.095","article-title":"miRNA-302 facilitates reprogramming of human adult hepatocytes into pancreatic islets-like cells in combination with a chemical defined media","volume":"453","author":"J Lu","year":"2014","journal-title":"Biochem Biophys Res Commun"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1007\/s11033-014-3054-4","article-title":"MiRNA-375 promotes beta pancreatic differentiation in human induced pluripotent stem (hiPS) cells","volume":"41","author":"R Lahmy","year":"2014","journal-title":"Mol Biol Rep"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1042\/bj20021545","article-title":"Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro","volume":"370","author":"CJ Marek","year":"2003","journal-title":"Biochem J"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1007\/s00125-006-0548-1","article-title":"Adenoviral overproduction of interleukin-1 receptor antagonist increases beta cell replication and mass in syngeneically transplanted islets, and improves metabolic outcome","volume":"50","author":"N T\u00e9llez","year":"2007","journal-title":"Diabetologia"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/978-1-62703-679-5_12","article-title":"Construction, production, and purification of recombinant adenovirus vectors","volume":"1089","author":"S Miravet","year":"2014","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"MW Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","article-title":"Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets","volume":"64","author":"CL Andersen","year":"2004","journal-title":"Cancer Res"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"e48093","DOI":"10.1371\/journal.pone.0048093","article-title":"Adenoviral Vectors Stimulate Glucagon Transcription in Human Mesenchymal Stem Cells Expressing Pancreatic Transcription Factors","volume":"7","author":"A Zaldumbide","year":"2012","journal-title":"PLOS ONE"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.neuron.2007.11.018","article-title":"Members of the miRNA-200 family regulate olfactory neurogenesis","volume":"57","author":"PS Choi","year":"2008","journal-title":"Neuron"},{"key":"ref31","first-page":"3156","article-title":"MicroRNA expression during chick embryo development","volume":"235","author":"DK Darnell","year":"2006","journal-title":"Dev Dyn Off Publ Am Assoc Anat"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/ng1744","article-title":"Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs","volume":"38","author":"R Yi","year":"2006","journal-title":"Nat Genet"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"7972","DOI":"10.1158\/0008-5472.CAN-07-1058","article-title":"Overexpression of the microRNA hsa-miR-200c leads to reduced expression of transcription factor 8 and increased expression of E-cadherin","volume":"67","author":"GJ Hurteau","year":"2007","journal-title":"Cancer Res"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1261\/rna.586807","article-title":"miR-200b mediates post-transcriptional repression of ZFHX1B","volume":"13","author":"NR Christoffersen","year":"2007","journal-title":"RNA N Y N"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.4161\/cc.7.20.6851","article-title":"MicroRNAs as regulators of epithelial-mesenchymal transition","volume":"7","author":"PA Gregory","year":"2008","journal-title":"Cell Cycle Georget Tex"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/j.celrep.2014.02.013","article-title":"De Novo Formation of Insulin-Producing \u201cNeo-\u03b2 Cell Islets\u201d from Intestinal Crypts","volume":"6","author":"Y-J Chen","year":"2014","journal-title":"Cell Rep"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.2337\/db12-1256","article-title":"Suppression of Epithelial-to-Mesenchymal Transitioning Enhances Ex Vivo Reprogramming of Human Exocrine Pancreatic Tissue Toward Functional Insulin-Producing-Like Cells","volume":"62","author":"MJ Lima","year":"2013","journal-title":"Diabetes"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.stem.2011.03.001","article-title":"Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency","volume":"8","author":"F Anokye-Danso","year":"2011","journal-title":"Cell Stem Cell"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1172\/JCI39104","article-title":"The basics of epithelial-mesenchymal transition","volume":"119","author":"R Kalluri","year":"2009","journal-title":"J Clin Invest"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"4875","DOI":"10.1091\/mbc.E08-05-0506","article-title":"Snail and Slug promote epithelial-mesenchymal transition through beta-catenin-T-cell factor-4-dependent expression of transforming growth factor-beta3","volume":"19","author":"D Medici","year":"2008","journal-title":"Mol Biol Cell"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1007\/s00335-006-0025-7","article-title":"Nonrestrictive developmental regulation of microRNA gene expression","volume":"17","author":"WM Strauss","year":"2006","journal-title":"Mamm Genome Off J Int Mamm Genome Soc"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"54","DOI":"10.2174\/2211536603666140522003220","article-title":"The Role of MicroRNAs in the Pancreatic Differentiation of Pluripotent Stem Cells","volume":"3","author":"N Francis","year":"2014","journal-title":"MicroRNA"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1039\/c3tx50030f","article-title":"The B-13 hepatocyte progenitor cell resists pluripotency induction and differentiation to non-hepatocyte cells","volume":"2","author":"EA Fairhall","year":"2013","journal-title":"Toxicol Res"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"e55064","DOI":"10.1371\/journal.pone.0055064","article-title":"MicroRNA Expression in Alpha and Beta Cells of Human Pancreatic Islets","volume":"8","author":"D Klein","year":"2013","journal-title":"PLOS ONE"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1038\/nm.3287","article-title":"Thioredoxin-interacting protein regulates insulin transcription through microRNA-204","volume":"19","author":"G Xu","year":"2013","journal-title":"Nat Med"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1038\/nature07299","article-title":"MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation","volume":"455","author":"Y Tay","year":"2008","journal-title":"Nature"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/nrg2971","article-title":"State-of-the-art gene-based therapies: the road ahead","volume":"12","author":"MA Kay","year":"2011","journal-title":"Nat Rev Genet"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1007\/s00125-011-2070-3","article-title":"In vivo genetic engineering of murine pancreatic beta cells mediated by single-stranded adeno-associated viral vectors of serotypes 6, 8 and 9","volume":"54","author":"V Jimenez","year":"2011","journal-title":"Diabetologia"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1089\/hum.2013.182","article-title":"Pancreatic transduction by helper-dependent adenoviral vectors via intraductal delivery","volume":"25","author":"M Morr\u00f3","year":"2014","journal-title":"Hum Gene Ther"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0145116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T11:41:09Z","timestamp":1540294869000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0145116"}},"subtitle":[],"editor":[{"given":"Marc","family":"Claret","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,12,21]]},"references-count":49,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2015,12,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0145116","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,21]]}}}