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(SMS)","volume":"2013","author":"P Mukadi","journal-title":"PLoS ONE"},{"key":"ref18","unstructured":"Programme National de Lutte contre le Paludisme: Plan strat\u00e9gique national de lutte contre le paludisme 2013\u20132015. 2013."},{"key":"ref19","unstructured":"Programme National de Lutte contre la Trypanosomiase Humaine Africaine: Rapport annuel des activit\u00e9s de lutte contre la Trypanosomiase humaine Africaine 2013. 2014."},{"key":"ref20","unstructured":"Central Intelligence Agency: The World Factbook 2013\u20132014. Available: https:\/\/www.cia.gov\/library\/publications\/the-world-factbook\/geos\/cg.html<\/ext-link>. 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