{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T08:16:39Z","timestamp":1785226599103,"version":"3.55.0"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T00:00:00Z","timestamp":1452470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0146686","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T13:58:13Z","timestamp":1452520693000},"page":"e0146686","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Essential Contribution of CD4+ T Cells to Antigen-Induced Nasal Hyperresponsiveness in Experimental Allergic Rhinitis"],"prefix":"10.1371","volume":"11","author":[{"given":"Tomoe","family":"Nishimura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Kaminuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayumi","family":"Saeki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Kitamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunie","family":"Matsuoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromichi","family":"Yonekawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Mori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takachika","family":"Hiroi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,1,11]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s11882-001-0007-5","article-title":"Mechanisms of allergic rhinitis","volume":"1","author":"JN Baraniuk","year":"2001","journal-title":"Curr Allergy Asthma Rep"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1007\/s11882-003-0049-y","article-title":"Preformed enzymes in mast cell granules and their potential role in allergic rhinitis","volume":"3","author":"SA Parikh","year":"2003","journal-title":"Curr Allergy Asthma Rep"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1513\/pats.201008-057RN","article-title":"Pathophysiology of allergic and nonallergic rhinitis","volume":"8","author":"B Sin","year":"2011","journal-title":"Proc Am, Thorac Soc"},{"key":"ref4","first-page":"65","article-title":"Evaluation of threshold criteria for the nasal histamine challenge test in perennial allergic rhinitis","volume":"15","author":"S Kanthawatana","year":"1997","journal-title":"Asian Pac J Allergy Immunol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1159\/000024054","article-title":"Neural hyperresponsiveness and nerve growth factor in allergic rhinitis","volume":"118","author":"AM Sanico","year":"1999","journal-title":"Int Arch Allergy Immunol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1365-2222.1987.tb02052.x","article-title":"Comparison of nasal responsiveness to histamine, methacholine and phentolamine in allergic rhinitis patients and controls","volume":"17","author":"R Gerth Van Wijk","year":"1987","journal-title":"Clin Allergy"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/BF00457450","article-title":"Seasonal variation of sensitivity of nasal mucosa in pollinosis","volume":"232","author":"A Konno","year":"1981","journal-title":"Arch Otorhinolaryngol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1164\/ajrccm\/144.3_Pt_1.642","article-title":"Antigen-provoked increase in histamine reactivity. Observations on mechanisms","volume":"144","author":"SM Walden","year":"1991","journal-title":"Am Rev Respir Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/S0091-6749(05)80017-X","article-title":"Intranasal beclomethasone inhibits antigen-induced nasal hyperresponsiveness to histamine","volume":"90","author":"FM Baroody","year":"1992","journal-title":"J Allergy Clin Immunol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1046\/j.1365-2222.1996.d01-395.x","article-title":"Relationship between nasal hyperreactivity, mediators and eosinophils in patients with perennial allergic rhinitis and controls","volume":"26","author":"C de Graaf-in t Veld","year":"1996","journal-title":"Clin Exp Allergy"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.1164\/ajrccm.151.6.7767538","article-title":"Cytokine immunoreactivity in seasonal rhinitis: regulation by a topical corticosteroid","volume":"151","author":"P Bradding","year":"1995","journal-title":"Am J Respir Crit Care Med"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/S0091-6749(98)70277-5","article-title":"Nasal eosinophilia and IL-5 mRNA expression in seasonal allergic rhinitis induced by natural allergen exposure: effect of topical corticosteroids","volume":"102","author":"K Masuyama","year":"1998","journal-title":"J Allergy Clin Immunol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1165\/ajrcmb.17.1.2696","article-title":"IL-13 mRNA and immunoreactivity in allergen-induced rhinitis: comparison with IL-4 expression and modulation by topical glucocorticoid therapy","volume":"17","author":"O Ghaffar","year":"1997","journal-title":"Am J Respir Cell Mol Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1067\/mai.2000.107398","article-title":"Local synthesis of epsilon germline gene transcripts, IL-4, and IL-13 in allergic nasal mucosa after ex vivo allergen exposure","volume":"106","author":"L Cameron","year":"2000","journal-title":"J Allergy Clin Immunol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1046\/j.1365-2222.2000.00998.x","article-title":"Cytokine expression during allergen-induced late nasal responses: IL-4 and IL-5 mRNA is expressed early (at 6 h) predominantly by eosinophils","volume":"30","author":"KT Nouri-Aria","year":"2000","journal-title":"Clin Exp Allergy"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1016\/S0091-6749(96)70205-1","article-title":"Grass pollen immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of cells expressing messenger RNA for interferon-gamma","volume":"97","author":"SR Durham","year":"1996","journal-title":"J Allergy Clin Immunol"},{"key":"ref17","first-page":"192","article-title":"Topical glucocorticosteroid (fluticasone propionate) inhibits cells expressing cytokine mRNA for interleukin-4 in the nasal mucosa in allergen-induced rhinitis","volume":"82","author":"K Masuyama","year":"1994","journal-title":"Immunology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1172\/JCI116633","article-title":"Influence of grass pollen immunotherapy on cellular infiltration and cytokine mRNA expression during allergen-induced late-phase cutaneous responses","volume":"92","author":"VA Varney","year":"1993","journal-title":"J Clin Invest"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1159\/000237003","article-title":"Changes in allergic inflammation associated with successful immunotherapy","volume":"107","author":"SR Durham","year":"1995","journal-title":"Int Arch Allergy Immunol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev.immunol.17.1.255","article-title":"Immunologic basis of antigen-induced airway hyperresponsiveness","volume":"17","author":"M Wills-Karp","year":"1999","journal-title":"Annu Rev Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"365","DOI":"10.2500\/108854198778612744","article-title":"Essential role of T lymphocytes in the development of allergen-driven airway hyperresponsiveness","volume":"19","author":"EW Gelfand","year":"1998","journal-title":"Allergy Asthma Proc"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1365-2222.2011.03847.x","article-title":"Selective down-regulation of Th2 cell-mediated airway inflammation in mice by pharmacological intervention of CCR4","volume":"42","author":"O Kaminuma","year":"2012","journal-title":"Clin Exp Allergy"},{"issue":"Suppl 1","key":"ref23","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1159\/000312130","article-title":"Eosinophils are required for the induction of bronchial hyperresponsiveness in a Th transfer model of BALB\/c background","volume":"152","author":"T Ohtomo","year":"2010","journal-title":"Int Arch Allergy Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1046\/j.0022-0477.2001.01248.x","article-title":"Transient contribution of mast cells to pulmonary eosinophilia but not to hyper-responsiveness","volume":"32","author":"K Ogawa","year":"2002","journal-title":"Clin Exp Allergy"},{"issue":"Suppl 2","key":"ref25","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1159\/000350565","article-title":"Existence of antigen-specific immunoglobulin E is not sufficient for allergic nasal eosinophil infiltration in mice","volume":"161","author":"T Nishimura","year":"2013","journal-title":"Int Arch Allergy Immunol"},{"issue":"Suppl 1","key":"ref26","doi-asserted-by":"crossref","first-page":"29","DOI":"10.2332\/allergolint.13-OA-0635","article-title":"Selective suppression of Th2 cell-mediated lung eosinophilic inflammation by anti-major facilitator super family domain containing 10 monoclonal antibody","volume":"63","author":"T Nishimura","year":"2014","journal-title":"Allergol Int"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1093\/intimm\/dxm049","article-title":"Selective suppression of Th2 cell-mediated airway eosinophilic inflammation by low-molecular weight CCR3 antagonist","volume":"19","author":"A Mori","year":"2005","journal-title":"Int Immunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1111\/j.1467-7652.2011.00613.x","article-title":"Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen-specific oral tolerance without bystander suppression","volume":"9","author":"K Suzuki","year":"2011","journal-title":"Plant Biotechnol J"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.imlet.2012.07.001","article-title":"Establishment of monoclonal antibodies against a novel eosinophil-specific cell surface molecule, major facilitator super family domain containing 10","volume":"147","author":"Y Motoi","year":"2012","journal-title":"Immunol Lett"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.jaci.2003.08.009","article-title":"Features of airway remodeling and eosinophilic inflammation in chronic rhinosinusitis: is the histopathology similar to asthma?","volume":"112","author":"JU Ponikau","year":"2003","journal-title":"J Allergy Clin Immunol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3198","DOI":"10.1002\/eji.201141521","article-title":"CD44 is critical for airway accumulation of antigen-specific Th2, but not Th1, cells induced by antigen challenge in mice","volume":"41","author":"S Katoh","year":"2011","journal-title":"Eur J Immunol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1177\/039463200902200424","article-title":"Clinical importance of eosinophil count in nasal fluid in patients with allergic and non-allergic rhinitis","volume":"22","author":"G Di Lorenzo","year":"2009","journal-title":"Int J Immunopathol Pharmacol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.jaci.2013.07.046","article-title":"Basophils and allergic inflammation","volume":"132","author":"MC Siracusa","year":"2013","journal-title":"J Allergy Clin Immunol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1111\/j.1398-9995.2011.02613.x","article-title":"Newly appreciated roles for basophils in allergy and protective immunity","volume":"66","author":"H Karasuyama","year":"2011","journal-title":"Allergy"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s00011-015-0800-6","article-title":"Bencycloquidium bromide inhibits nasal hypersecretion in a rat model of allergic rhinitis","volume":"64","author":"R Long","year":"2015","journal-title":"Inflamm Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/pbi.12007","article-title":"Oral immunotherapy with transgenic rice seed containing destructed Japanese cedar pollen allergens, Cry j 1 and Cry j 2, against Japanese cedar pollinosis","volume":"11","author":"Y Wakasa","year":"2013","journal-title":"Plant Biotechnol J"},{"issue":"Suppl 1","key":"ref37","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2332\/allergolint.13-LE-0634","article-title":"Antigen-induced mixed and separated inflammation in murine upper and lower airways","volume":"63","author":"M Saeki","year":"2014","journal-title":"Allergol Int"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.jaci.2012.02.013","article-title":"A critical role of IL-33 in experimental allergic rhinitis","volume":"130","author":"Y Haenuki","year":"2012","journal-title":"J Allergy Clin Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1046\/j.1365-2567.2001.01253.x","article-title":"Allergen-induced murine upper airway inflammation: local and systemic changes in murine experimental allergic rhinitis","volume":"104","author":"H Saito","year":"2001","journal-title":"Immunology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1465-9921-10-61","article-title":"Does unrestrained single-chamber plethysmography provide a valid assessment of airway responsiveness in allergic BALB\/c mice?","volume":"10","author":"Q Zhang","year":"2009","journal-title":"Respir Res"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e71","DOI":"10.2500\/ajra.2012.26.3743","article-title":"The effect of topical FK506 (tacrolimus) in a mouse model of allergic rhinitis","volume":"26","author":"JH Shin","year":"2012","journal-title":"Am J Rhinol Allergy"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"e103540","DOI":"10.1371\/journal.pone.0103540","article-title":"Nasal sensitization with ragweed pollen induces local-allergic-rhinitis-like symptoms in mice","volume":"9","author":"Y Kato","year":"2014","journal-title":"PLOS ONE"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/j.jaci.2015.03.001","article-title":"Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed","volume":"136","author":"J Bouley","year":"2015","journal-title":"J Allergy Clin Immunol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0014-2999(03)02185-X","article-title":"Kinins are involved in the development of allergic nasal hyperresponsiveness in guinea pigs","volume":"476","author":"S Sugahara","year":"2003","journal-title":"Eur J Pharmacol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1111\/j.1365-2222.2008.03097.x","article-title":"Important roles of tachykinins in the development of allergic nasal hyperresponsiveness in guinea-pigs","volume":"39","author":"T Nabe","year":"2009","journal-title":"Clin Exp Allergy"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0165-5728(98)00025-3","article-title":"Human lymphocytes express substance P and its receptor","volume":"86","author":"JP Lai","year":"1998","journal-title":"J Neuroimmunol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1128\/IAI.00358-08","article-title":"Interleukin-12 (IL-12) and IL-23 induction of substance p synthesis in murine T cells and macrophages is subject to IL-10 and transforming growth factor beta regulation","volume":"76","author":"A Blum","year":"2008","journal-title":"Infect Immun"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.neulet.2008.01.053","article-title":"Expression of preprotachykinin-A mRNA isoforms and substance P production in T lymphocytes of human healthy subjects","volume":"434","author":"L Cantalupo","year":"2008","journal-title":"Neurosci Lett"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0192-0561(97)00085-4","article-title":"Airway hyperresponsiveness: first eosinophils and then neuropeptides","volume":"19","author":"AD Kraneveld","year":"1997","journal-title":"Int J Immunopharmacol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.molmed.2005.02.002","article-title":"The role of eosinophils in the pathogenesis of asthma","volume":"11","author":"AB Kay","year":"2005","journal-title":"Trends Mol Med"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1183\/09059180.00004013","article-title":"Anti-interleukin-5 therapy in severe asthma","volume":"22","author":"G Garcia","year":"2013","journal-title":"Eur Respir Rev"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1465-9921-14-8","article-title":"Inhibition of neutrophil elastase attenuates airway hyperresponsiveness and inflammation in a mouse model of secondary allergen challenge: neutrophil elastase inhibition attenuates allergic airway responses","volume":"14","author":"H Koga","year":"2013","journal-title":"Respir Res"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1365-2222.1993.tb00318.x","article-title":"Neutrophil-induced human bronchial hyperresponsiveness in vitro\u2014pharmacological modulation","volume":"23","author":"JM Hughes","year":"1993","journal-title":"Clin Exp Allergy"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0279797","type":"expression_of_concern","label":"Expression of concern","source":"retraction-watch","updated":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"record-id":"44258"},{"DOI":"10.1371\/journal.pone.0279797","type":"expression_of_concern","label":"Expression of concern","source":"publisher","updated":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000}}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0146686","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T22:38:23Z","timestamp":1540334303000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0146686"}},"subtitle":[],"editor":[{"given":"Junji","family":"Yodoi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,1,11]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,1,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0146686","relation":{"expression_of_concern":[{"id-type":"doi","id":"10.1371\/journal.pone.0279797","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,11]]}}}