{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:30:21Z","timestamp":1789741821227,"version":"4.0.1"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0148367","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T14:05:04Z","timestamp":1454508304000},"page":"e0148367","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene"],"prefix":"10.1371","volume":"11","author":[{"given":"Dexi","family":"Bi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzhou","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cui","family":"Tai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewan M.","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiru","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixin","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumar","family":"Rajakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Yu","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,2,3]]},"reference":[{"issue":"9","key":"ref1","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/nrmicro1235","article-title":"Mobile genetic elements: the agents of open source evolution","volume":"3","author":"LS Frost","year":"2005","journal-title":"Nat Rev Microbiol"},{"issue":"9","key":"ref2","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1099\/jmm.0.009142-0","article-title":"<italic>Pseudomonas aeruginosa<\/italic>\u2014a phenomenon of bacterial resistance","volume":"58","author":"T Strateva","year":"2009","journal-title":"J Med Microbiol"},{"issue":"23","key":"ref3","doi-asserted-by":"crossref","first-page":"7495","DOI":"10.1128\/jb.174.23.7495-7499.1992","article-title":"Chromosomal insertion sites for phages and plasmids","volume":"174","author":"AM Campbell","year":"1992","journal-title":"J Bacteriol"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/JB.186.2.518-534.2004","article-title":"Sequence analysis of the mobile genome island pKLC102 of <italic>Pseudomonas aeruginosa<\/italic> C","volume":"186","author":"J Klockgether","year":"2004","journal-title":"J Bacteriol"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1128\/AAC.9.5.810","article-title":"Resistance plasmids of <italic>Pseudomonas aeruginosa<\/italic>: change from conjugative to nonconjugative in a hospital population","volume":"9","author":"TR Korfhagen","year":"1976","journal-title":"Antimicrob Agents Chemother"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1006\/jmbi.1994.1404","article-title":"Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis","volume":"239","author":"W Pansegrau","year":"1994","journal-title":"J Mol Biol"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/jac\/dkt311","article-title":"Characterization of plasmid pAX22, encoding VIM-1 metallo-beta-lactamase, reveals a new putative mechanism of In70 integron mobilization","volume":"69","author":"V Di Pilato","year":"2014","journal-title":"J Antimicrob Chemother"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1476-0711-12-29","article-title":"Analysis of integrons and associated gene cassettes in clinical isolates of multidrug resistant <italic>Pseudomonas aeruginosa<\/italic> from Southwest Nigeria","volume":"12","author":"BT Odumosu","year":"2013","journal-title":"Ann Clin Microbiol Antimicrob"},{"issue":"8","key":"ref9","doi-asserted-by":"crossref","first-page":"e70557","DOI":"10.1371\/journal.pone.0070557","article-title":"Identification and characterization of a novel <italic>aac(6&apos;)<\/italic>-<italic>Iag<\/italic> associated with the <italic>bla<\/italic><sub>IMP-1<\/sub>-integron in a multidrug-resistant <italic>Pseudomonas aeruginosa<\/italic>","volume":"8","author":"K Kobayashi","year":"2013","journal-title":"PLoS One"},{"issue":"8","key":"ref10","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.1128\/AAC.00423-13","article-title":"Complete sequence of pOZ176, a 500-kilobase IncP-2 plasmid encoding IMP-9-mediated carbapenem resistance, from outbreak isolate <italic>Pseudomonas aeruginosa<\/italic> 96","volume":"57","author":"J Xiong","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"issue":"8","key":"ref11","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1093\/jac\/dkt094","article-title":"Complete sequence of two KPC-harbouring plasmids from <italic>Pseudomonas aeruginosa<\/italic>","volume":"68","author":"T Naas","year":"2013","journal-title":"J Antimicrob Chemother"},{"issue":"7","key":"ref12","doi-asserted-by":"crossref","first-page":"3405","DOI":"10.1128\/AAC.02312-12","article-title":"The clinical isolate <italic>Pseudomonas aeruginosa<\/italic> MMA83 carries two copies of the <italic>bla<\/italic><sub>NDM-1<\/sub> gene in a novel genetic context","volume":"57","author":"B Jovcic","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.plasmid.2007.01.001","article-title":"Sequence of plasmid pBS228 and reconstruction of the IncP-1alpha phylogeny","volume":"58","author":"AS Haines","year":"2007","journal-title":"Plasmid"},{"issue":"14","key":"ref14","doi-asserted-by":"crossref","first-page":"4728","DOI":"10.1128\/JB.187.14.4728-4738.2005","article-title":"The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions","volume":"187","author":"AS Haines","year":"2005","journal-title":"J Bacteriol"},{"issue":"9","key":"ref15","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.1128\/AAC.01093-07","article-title":"Complete Sequence of p07-406, a 24,179-base-pair plasmid harboring the <italic>bla<\/italic><sub>VIM-7<\/sub> metallo-beta-lactamase gene in a <italic>Pseudomonas aeruginosa<\/italic> isolate from the United States","volume":"52","author":"H Li","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1128\/IAI.00621-09","article-title":"Pathogenicity islands PAPI-1 and PAPI-2 contribute individually and synergistically to the virulence of <italic>Pseudomonas aeruginosa<\/italic> strain PA14","volume":"78","author":"EM Harrison","year":"2010","journal-title":"Infect Immun"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.plasmid.2011.03.002","article-title":"Nucleotide sequence of <italic>Pseudomonas aeruginosa<\/italic> conjugative plasmid pUM505 containing virulence and heavy-metal resistance genes","volume":"66","author":"MI Ramirez-Diaz","year":"2011","journal-title":"Plasmid"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1007\/s00128-010-0162-2","article-title":"Plasmid-mediated biodegradation of the anionic surfactant sodium dodecyl sulphate, by <italic>Pseudomonas aeruginosa<\/italic> S7","volume":"86","author":"D Yeldho","year":"2011","journal-title":"Bull Environ Contam Toxicol"},{"issue":"23","key":"ref19","doi-asserted-by":"crossref","first-page":"4636","DOI":"10.1093\/nar\/27.23.4636","article-title":"Improved microbial gene identification with GLIMMER","volume":"27","author":"AL Delcher","year":"1999","journal-title":"Nucleic Acids Res"},{"key":"ref20","article-title":"Prokaryotic gene prediction using GeneMark and GeneMark","author":"M Borodovsky","year":"2003","journal-title":"hmm. Curr Protoc Bioinformatics"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/1471-2105-11-119","article-title":"Prodigal: prokaryotic gene recognition and translation initiation site identification","volume":"11","author":"D Hyatt","year":"2010","journal-title":"BMC Bioinformatics"},{"issue":"10","key":"ref22","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"K Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"issue":"5","key":"ref23","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"RC Edgar","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1093\/nar\/gnj005","article-title":"A novel strategy for the identification of genomic islands by comparative analysis of the contents and contexts of tRNA sites in closely related bacteria","volume":"34","author":"HY Ou","year":"2006","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"e8842","DOI":"10.1371\/journal.pone.0008842","article-title":"Complete genome sequence of the multiresistant taxonomic outlier <italic>Pseudomonas aeruginosa<\/italic> PA7","volume":"5","author":"PH Roy","year":"2010","journal-title":"PLoS One"},{"issue":"20","key":"ref26","doi-asserted-by":"crossref","first-page":"5839","DOI":"10.1093\/nar\/gkl732","article-title":"<italic>Phage_Finder<\/italic>: automated identification and classification of prophage regions in complete bacterial genome sequences","volume":"34","author":"DE Fouts","year":"2006","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.tim.2008.11.003","article-title":"Genomic islands are dynamic, ancient integrative elements in bacterial evolution","volume":"17","author":"EF Boyd","year":"2009","journal-title":"Trends Microbiol"},{"issue":"6799","key":"ref28","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1038\/35023079","article-title":"Complete genome sequence of <italic>Pseudomonas aeruginosa<\/italic> PAO1, an opportunistic pathogen","volume":"406","author":"CK Stover","year":"2000","journal-title":"Nature"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/plas.1995.1001","article-title":"Isolation and characterization of the replicon of a <italic>Thiobacillus intermedius<\/italic> plasmid","volume":"33","author":"RS English","year":"1995","journal-title":"Plasmid"},{"issue":"6887","key":"ref30","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/417459a","article-title":"Comparison of the genomes of two <italic>Xanthomonas<\/italic> pathogens with differing host specificities","volume":"417","author":"AC da Silva","year":"2002","journal-title":"Nature"},{"issue":"6","key":"ref31","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1128\/JB.187.6.2113-2126.2005","article-title":"Comparative genomic analysis of the pPT23A plasmid family of <italic>Pseudomonas syringae<\/italic>","volume":"187","author":"Y Zhao","year":"2005","journal-title":"J Bacteriol"},{"issue":"suppl 1","key":"ref32","doi-asserted-by":"crossref","first-page":"D606","DOI":"10.1093\/nar\/gkq908","article-title":"TADB: a web-based resource for Type 2 toxin-antitoxin loci in bacteria and archaea","volume":"39","author":"Y Shao","year":"2011","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"ref33","doi-asserted-by":"crossref","first-page":"3196","DOI":"10.1128\/jb.174.10.3196-3203.1992","article-title":"Genetic analysis of bacterial acetyltransferases: identification of amino acids determining the specificities of the aminoglycoside 6&apos;-N-acetyltransferase Ib and IIa proteins","volume":"174","author":"PN Rather","year":"1992","journal-title":"J Bacteriol"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1128\/MMBR.00056-13","article-title":"Integrons: past, present, and future","volume":"78","author":"MR Gillings","year":"2014","journal-title":"Microbiol Mol Biol Rev"},{"issue":"3","key":"ref35","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.ygeno.2012.06.009","article-title":"The limitations of draft assemblies for understanding prokaryotic adaptation and evolution","volume":"100","author":"N Ricker","year":"2012","journal-title":"Genomics"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0148367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T15:13:26Z","timestamp":1540221206000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0148367"}},"subtitle":[],"editor":[{"given":"Finbarr","family":"Hayes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,2,3]]},"references-count":35,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,2,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0148367","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,3]]}}}