{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:11:46Z","timestamp":1790809906725,"version":"4.1.0"},"reference-count":26,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T00:00:00Z","timestamp":1461715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0148749","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T13:36:44Z","timestamp":1461764204000},"page":"e0148749","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Mutagenesis of mNeptune Red-Shifts Emission Spectrum to 681-685 nm"],"prefix":"10.1371","volume":"11","author":[{"given":"ZhaoYang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZhiPing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LiJun","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZongQiang","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JiaoYu","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DianBing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian-En","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,4,27]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/86684","article-title":"A clearer vision for in vivo imaging","volume":"19","author":"R Weissleder","year":"2001","journal-title":"Nature biotechnology"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nm0103-123","article-title":"Shedding light onto live molecular targets","volume":"9","author":"R Weissleder","year":"2003","journal-title":"Nature medicine"},{"issue":"10","key":"ref3","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/nmeth.1501","article-title":"Near-infrared fluorescent proteins","volume":"7","author":"D Shcherbo","year":"2010","journal-title":"Nature methods"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1038\/srep01847","article-title":"Extended Stokes Shift in Fluorescent Proteins: Chromophore-Protein Interactions in a Near-Infrared TagRFP675 Variant","volume":"3","author":"KD Piatkevich","year":"2013","journal-title":"Scientific reports"},{"issue":"7","key":"ref5","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nmeth1062","article-title":"Bright monomeric red fluorescent protein with an extended fluorescence lifetime","volume":"4","author":"EM Merzlyak","year":"2007","journal-title":"Nature methods"},{"issue":"7","key":"ref6","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1002\/pro.654","article-title":"Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore","volume":"20","author":"NV Pletneva","year":"2011","journal-title":"Protein Science"},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.chembiol.2009.10.009","article-title":"Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals","volume":"16","author":"MZ Lin","year":"2009","journal-title":"Chemistry & biology"},{"issue":"43","key":"ref8","doi-asserted-by":"crossref","first-page":"28980","DOI":"10.1074\/jbc.M800599200","article-title":"A crystallographic study of bright far-red fluorescent protein mKate reveals pH-induced <italic>cis-trans<\/italic> isomerization of the chromophore","volume":"283","author":"S Pletnev","year":"2008","journal-title":"Journal of Biological Chemistry"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","article-title":"Site-directed mutagenesis by overlap extension using the polymerase chain reaction","volume":"77","author":"SN Ho","year":"1989","journal-title":"Gene"},{"key":"ref10","article-title":"The S28H mutation on mNeptune generates a brighter near-infrared monomeric fluorescent protein with improved quantum yield and pH-stability","author":"Z-Y Li","year":"2014","journal-title":"Acta biochimica et biophysica Sinica"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1042\/BJ20081949","article-title":"Far-red fluorescent tags for protein imaging in living tissues","volume":"418","author":"D Shcherbo","year":"2009","journal-title":"Biochem J"},{"issue":"22","key":"ref12","doi-asserted-by":"crossref","first-page":"11990","DOI":"10.1073\/pnas.97.22.11990","article-title":"The structure of the chromophore within DsRed, a red fluorescent protein from coral","volume":"97","author":"LA Gross","year":"2000","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref13","article-title":"Methods of Biochemical Analysis, Green Fluorescent Protein: Properties, Applications and Protocols","author":"M Chalfie","year":"2005"},{"issue":"8","key":"ref14","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1021\/j100678a001","article-title":"Measurement of photoluminescence quantum yields. Review","volume":"75","author":"GA Crosby","year":"1971","journal-title":"The Journal of Physical Chemistry"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1016\/S0006-3495(97)78307-3","article-title":"Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy","volume":"73","author":"GH Patterson","year":"1997","journal-title":"Biophysical journal"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/bioinformatics\/bti770","article-title":"The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling","volume":"22","author":"K Arnold","year":"2006","journal-title":"Bioinformatics"},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1107\/S0907444912020598","article-title":"Structural basis for bathochromic shift of fluorescence in far-red fluorescent proteins eqFP650 and eqFP670","volume":"68","author":"S Pletnev","year":"2012","journal-title":"Acta Crystallographica Section D: Biological Crystallography"},{"issue":"9","key":"ref18","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/nmeth1083","article-title":"Bright far-red fluorescent protein for whole-body imaging","volume":"4","author":"D Shcherbo","year":"2007","journal-title":"Nature methods"},{"issue":"48","key":"ref19","doi-asserted-by":"crossref","first-page":"16745","DOI":"10.1073\/pnas.0407752101","article-title":"Evolution of new nonantibody proteins via iterative somatic hypermutation","volume":"101","author":"L Wang","year":"2004","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1002\/pro.66","article-title":"Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein","volume":"18","author":"X Shu","year":"2009","journal-title":"Protein science: a publication of the Protein Society"},{"issue":"12","key":"ref21","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1038\/nbt1264","article-title":"Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library","volume":"24","author":"MA Mena","year":"2006","journal-title":"Nature biotechnology"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1038\/nprot.2006.223","article-title":"Whole-body imaging with fluorescent proteins","volume":"1","author":"RM Hoffman","year":"2006","journal-title":"Nature protocols"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1038\/nprot.2006.109","article-title":"Subcellular imaging in the live mouse","volume":"1","author":"RM Hoffman","year":"2006","journal-title":"Nature protocols"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1038\/nprot.2006.119","article-title":"Color-coded fluorescence imaging of tumor-host interactions","volume":"1","author":"RM Hoffman","year":"2006","journal-title":"Nature protocols"},{"issue":"10","key":"ref25","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/nrc1717","article-title":"The multiple uses of fluorescent proteins to visualize cancer in vivo","volume":"5","author":"RM Hoffman","year":"2005","journal-title":"Nature Reviews Cancer"},{"issue":"11","key":"ref26","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/nnano.2009.326","article-title":"Bioimaging: Second window for in vivo imaging","volume":"4","author":"AM Smith","year":"2009","journal-title":"Nature Nanotechnology"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0148749","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T09:05:19Z","timestamp":1540026319000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0148749"}},"subtitle":[],"editor":[{"given":"Robert M.","family":"Hoffman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,4,27]]},"references-count":26,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,4,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0148749","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,27]]}}}