{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T16:55:15Z","timestamp":1776272115002,"version":"3.50.1"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2016,2,18]],"date-time":"2016-02-18T00:00:00Z","timestamp":1455753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0149425","type":"journal-article","created":{"date-parts":[[2016,2,18]],"date-time":"2016-02-18T21:30:15Z","timestamp":1455831015000},"page":"e0149425","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["MiR-449a Affects Epithelial Proliferation during the Pseudoglandular and Canalicular Phases of Avian and Mammal Lung Development"],"prefix":"10.1371","volume":"11","author":[{"given":"Ethan L.","family":"Sanford","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwong W.","family":"Choy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia K.","family":"Donahoe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam A.","family":"Tracy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regis","family":"Hila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Loscertales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Longoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,2,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.resp.2013.04.015","article-title":"Current concepts on the pathogenesis and etiology of congenital diaphragmatic hernia","volume":"189","author":"JJ Greer","year":"2013","journal-title":"Respir Physiol Neurobiol. Elsevier B.V."},{"key":"ref2","first-page":"1993","article-title":"SourceGeneReviewsTM","author":"BR Pober","year":"2010"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"335","DOI":"10.2350\/07-06-0290.1","article-title":"Key mechanisms of early lung development","volume":"10","author":"J Kimura","year":"2007","journal-title":"Pediatr Dev Pathol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1242\/dev.02310","article-title":"Regulation of early lung morphogenesis: questions, facts and controversies","volume":"133","author":"W V Cardoso","year":"2006","journal-title":"Development"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1242\/dev.010504","article-title":"Chick pulmonary Wnt5a directs airway and vascular tubulogenesis","volume":"135","author":"M Loscertales","year":"2008","journal-title":"Development"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: target recognition and regulatory functions","volume":"136","author":"DP Bartel","year":"2009","journal-title":"Cell"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"9667","DOI":"10.1073\/pnas.0703820104","article-title":"Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5\u2019 UTR as in the 3' UTR","volume":"104","author":"JR Lytle","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1002\/ppul.22739","article-title":"MicroRNAs and lung development","volume":"48","author":"N Khoshgoo","year":"2013","journal-title":"Pediatr Pulmonol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e10854","DOI":"10.1371\/journal.pone.0010854","article-title":"MicroRNA networks in mouse lung organogenesis","volume":"5","author":"J Dong","year":"2010","journal-title":"PLoS One"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.1073\/pnas.0510839103","article-title":"Dicer function is essential for lung epithelium morphogenesis","volume":"103","author":"KS Harris","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.cell.2008.02.019","article-title":"Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters","volume":"132","author":"A Ventura","year":"2008","journal-title":"Cell"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.ydbio.2007.08.007","article-title":"Transgenic over-expression of the microRNA miR-17-92 cluster promotes proliferation and inhibits differentiation of lung epithelial progenitor cells","volume":"310","author":"Y Lu","year":"2007","journal-title":"Dev Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1513\/pats.200710-162DR","article-title":"Epithelial progenitor cells of the embryonic lung and the role of microRNAs in their proliferation","volume":"5","author":"Y Lu","year":"2008","journal-title":"Proc Am Thorac Soc"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1152\/physiolgenomics.90268.2008","article-title":"MicroRNA-127 modulates fetal lung development","volume":"37","author":"M Bhaskaran","year":"2009","journal-title":"Physiol Genomics"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1136\/bmj.1.5325.230","article-title":"Hypoplasia of lung with congenital diaphragmatic hernia","volume":"1","author":"W Areechon","year":"1963","journal-title":"Br Med J"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1002\/bjs.1800580507","article-title":"Lung hypoplasia in congenital diaphragmatic hernia. A quantitative study of airway, artery, and alveolar development","volume":"58","author":"M Kitagawa","year":"1971","journal-title":"Br J Surg"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.yexmp.2012.09.010","article-title":"Premature differentiation of vascular smooth muscle cells in human congenital diaphragmatic hernia","volume":"94","author":"I Sluiter","year":"2013","journal-title":"Exp Mol Pathol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/ajmg.c.30124","article-title":"Lung development and implications for hypoplasia found in congenital diaphragmatic hernia","volume":"145C","author":"TB Kinane","year":"2007","journal-title":"Am J Med Genet C Semin Med Genet"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0925-4773(99)00325-1","article-title":"The molecular basis of lung morphogenesis","author":"D Warburton","year":"2000","journal-title":"Mech Dev"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0070-2153(10)90003-3","article-title":"Lung organogenesis","volume":"90","author":"D Warburton","year":"2010","journal-title":"Curr Top Dev Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"2978","DOI":"10.1073\/pnas.1121621109","article-title":"Congenital diaphragmatic hernia candidate genes derived from embryonic transcriptomes","volume":"109","author":"MK Russell","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"12450","DOI":"10.1073\/pnas.1412509111","article-title":"Molecular pathogenesis of congenital diaphragmatic hernia revealed by exome sequencing, developmental data, and bioinformatics","volume":"111","author":"M Longoni","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1093\/nar\/30.1.207","article-title":"Gene Expression Omnibus: NCBI gene expression and hybridization array data repository","volume":"30","author":"R Edgar","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"ref24","article-title":"Atlas of Embryonic Development","author":"K Theiler","year":"1989"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/jmor.1050880104","article-title":"A series of normal stages in the development of the chick embryo","volume":"88","author":"V Hamburger","year":"1951","journal-title":"J Morphol"},{"key":"ref26","first-page":"652","article-title":"Antioxidants reverse fetal rat pulmonary hypoplasia in vitro","volume":"XLVIII","author":"BA Pacheco","year":"1997","journal-title":"Surgical Forum"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1006\/meth.1998.0595","article-title":"Targeted gene misexpression in chick limb buds using avian replication-competent retroviruses","volume":"14","author":"M Logan","year":"1998","journal-title":"Methods"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0091-679X(08)60629-9","article-title":"Manipulating gene expression with replication-competent retroviruses","volume":"51","author":"BA Morgan","year":"1996","journal-title":"Methods Cell Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1046\/j.1440-169X.1999.413430.x","article-title":"Fate mapping study of the endoderm in the posterior part of the 1.5-day-old chick embryo","volume":"41","author":"S Matsushita","year":"1999","journal-title":"Dev Growth Differ"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"4579","DOI":"10.4161\/cc.9.22.13870","article-title":"MicroRNA-449a levels increase by several orders of magnitude during mucociliary differentiation of airway epithelia","volume":"9","author":"M Liz\u00e9","year":"2010","journal-title":"Cell Cycle"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/cdd.2009.188","article-title":"E2F1-inducible microRNA 449a\/b suppresses cell proliferation and promotes apoptosis","volume":"17","author":"M Liz\u00e9","year":"2010","journal-title":"Cell Death Differ"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.1101\/gad.1819009","article-title":"miR-449a and miR-449b are direct transcriptional targets of E2F1 and negatively regulate pRb-E2F1 activity through a feedback loop by targeting CDK6 and CDC25A","volume":"23","author":"X Yang","year":"2009","journal-title":"Genes Dev"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/ncb2241","article-title":"Control of vertebrate multiciliogenesis by miR-449 through direct repression of the Delta\/Notch pathway","volume":"13","author":"B Marcet","year":"2011","journal-title":"Nat Cell Biol. Nature Publishing Group"},{"key":"ref34","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a014282","article-title":"Oncogenic mechanisms in Burkitt lymphoma","volume":"4","author":"R Schmitz","year":"2014","journal-title":"Cold Spring Harb Perspect Med"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1158\/1541-7786.MCR-13-0536","article-title":"The N-myc Oncogene: Maximizing its Targets, Regulation, and Therapeutic Potential","volume":"12","author":"H Beltran","year":"2014","journal-title":"Mol Cancer Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1056\/NEJM198510313131802","article-title":"Association of multiple copies of the N-myc oncogene with rapid progression of neuroblastomas","volume":"313","author":"RC Seeger","year":"1985","journal-title":"N Engl J Med"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1038\/sj.onc.1208997","article-title":"Combined microarray analysis of small cell lung cancer reveals altered apoptotic balance and distinct expression signatures of MYC family gene amplification","volume":"25","author":"YH Kim","year":"2006","journal-title":"Oncogene"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2235","DOI":"10.1101\/gad.6.12a.2235","article-title":"Loss of N-myc function results in embryonic lethality and failure of the epithelial component of the embryo to develop","volume":"6","author":"BR Stanton","year":"1992","journal-title":"Genes Dev"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1242\/dev.01678","article-title":"Nmyc plays an essential role during lung development as a dosage-sensitive regulator of progenitor cell proliferation and differentiation","volume":"132","author":"T Okubo","year":"2005","journal-title":"Development"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"L168","DOI":"10.1152\/ajplung.00299.2014","article-title":"Defective parasympathetic innervation is associated with airway branching abnormalities in experimental CDH","volume":"309","author":"J Rhodes","year":"2015","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"10482","DOI":"10.1073\/pnas.152238499","article-title":"Early restriction of peripheral and proximal cell lineages during formation of the lung","volume":"99","author":"A-KT Perl","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"29532","DOI":"10.1074\/jbc.M801565200","article-title":"-Secretase Activation of Notch Signaling Regulates the Balance of Proximal and Distal Fates in Progenitor Cells of the Developing Lung","volume":"283","author":"P-N Tsao","year":"2008","journal-title":"J Biol Chem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/ejhg.2010.225","article-title":"Dissection of the MYCN locus in Feingold syndrome and isolated oesophageal atresia","volume":"19","author":"M Cognet","year":"2011","journal-title":"Eur J Hum Genet"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0149425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T18:50:23Z","timestamp":1567623023000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0149425"}},"subtitle":[],"editor":[{"given":"David","family":"Warburton","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,2,18]]},"references-count":43,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,2,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0149425","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,18]]}}}