{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T03:24:39Z","timestamp":1781321079016,"version":"3.54.1"},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2016,3,3]],"date-time":"2016-03-03T00:00:00Z","timestamp":1456963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0150455","type":"journal-article","created":{"date-parts":[[2016,3,3]],"date-time":"2016-03-03T18:53:50Z","timestamp":1457031230000},"page":"e0150455","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Trichoderma harzianum Produces a New Thermally Stable Acid Phosphatase, with Potential for Biotechnological Application"],"prefix":"10.1371","volume":"11","author":[{"given":"Amanda Ara\u00fajo","family":"Souza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa Oliveira","family":"Leit\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Henrique","family":"Ramada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azadeh","family":"Mehdad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphaela de Castro","family":"Georg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cirano Jos\u00e9","family":"Ulh\u00f4a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia Maria de","family":"Freitas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,3,3]]},"reference":[{"key":"ref1","article-title":"Understanding Phosphorus and its Use in Agriculture","author":"AE Johnston","year":"2000"},{"issue":"7","key":"ref2","doi-asserted-by":"crossref","first-page":"2926","DOI":"10.1128\/AEM.65.7.2926-2933.1999","article-title":"Solubilization of phosphates and micronutrientes by the plant-growth promoting and biocontrol fungus <italic>Trichoderma harzianum<\/italic> Rifai 1295\u201322","volume":"65","author":"C Altomare","year":"1999","journal-title":"Appl Environ Microbiol"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1007\/s00284-011-9888-2","article-title":"Effect of Abiotic Stress on Phosphate Solubilization by Biocontrol Fungus <italic>Trichoderma<\/italic> sp","volume":"62","author":"R Rawat","year":"2011","journal-title":"Curr Microbiol"},{"key":"ref4","article-title":"Inorganic Phosphate as an Important Regulator of Phosphatases","author":"CF Dick","year":"2011","journal-title":"Enzyme Res"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.1007\/s12010-012-9694-8","article-title":"A Molecular Description of Acid Phosphatase","volume":"167","author":"A Anand","year":"2012","journal-title":"Appl Biochem Biotechnol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1111\/j.1399-3054.1994.tb02539.x","article-title":"The role of acid phosphatases in plan phosphorus metabolism","volume":"90","author":"SMG Duff","year":"1994","journal-title":"Physiol Plant"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.jmb.2004.03.057","article-title":"Crystal structure of a heat-resilient phytase from <italic>Aspergillus fumigatus<\/italic>, carrying a phosphorylated histidine","volume":"339","author":"T Xiang","year":"2004","journal-title":"J Mol Biol"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.bbrc.2010.06.024","article-title":"The structure of <italic>Aspergillus niger<\/italic> phytase PhyA in complex with a phytate mimetic","volume":"397","author":"AJ Oakley","year":"2010","journal-title":"Biochem Biophys Res Commun"},{"issue":"6","key":"ref9","first-page":"361","article-title":"Purification and Biochemical Characterization of acid phosphatase from <italic>Vigna aconitifolia<\/italic>","volume":"5","author":"MA Al-Omar","year":"2010","journal-title":"Plant Physiol"},{"issue":"7","key":"ref10","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S0981-9428(98)80173-3","article-title":"Purification and characterization of multiple forms of soybean seed acid phosphatases","volume":"36","author":"CV Ferreira","year":"1998","journal-title":"Plant Physiol Biochem"},{"key":"ref11","first-page":"165","article-title":"Dynamics of Bone and Cartilage Metabolism: Principles and Clinical Applications","author":"H Kaija","year":"2006"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.cellbi.2003.12.008","article-title":"Acid phosphatase activity in gerbil prostate: comparative study in male and female during postnatal development","volume":"28","author":"AM Custodio","year":"2004","journal-title":"Cell Biol Int"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1042\/BA20030208","article-title":"Characterization and properties of acid phosphatases with phytase activity produced by <italic>Aspergillus caespitosus<\/italic>","volume":"40","author":"LHS Guimar\u00e3es","year":"2004","journal-title":"Biotechnol Appl Biochem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jobm.201200218","article-title":"Purification and characterization of a novel acid phosphatase from the split gill mushroom <italic>Schizophyllum commune<\/italic>","volume":"53","author":"GQ Zhang","year":"2013","journal-title":"J Basic Microbiol"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1590\/S1517-83822010005000001","article-title":"Phosphate solubilization potential and phosphatase activity of rhizospheric <italic>Trichoderma<\/italic> spp","volume":"41","author":"A Kapri","year":"2010","journal-title":"Braz J Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"10438","DOI":"10.3390\/ijms140510438","article-title":"Human prostatic acid phosphatase: structure, function and regulation","volume":"14","author":"S Muniyan","year":"2013","journal-title":"Int J Mol Sci"},{"issue":"9","key":"ref17","doi-asserted-by":"crossref","first-page":"e73072","DOI":"10.1371\/journal.pone.0073072","article-title":"Transmembrane Prostatic Acid Phosphatase (TMPAP) Interacts with Snapin and Deficient Mice Develop Prostate Adenocarcinoma","volume":"8","author":"IB Quintero","year":"2013","journal-title":"PLoS One"},{"key":"ref18","first-page":"720","article-title":"The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broilers chickens fed corn-soubean diets","volume":"75","author":"S Sebastian","year":"1996","journal-title":"Poult Sci"},{"key":"ref19","first-page":"751","article-title":"Microbial phytase activity and their role in organic P mineralization","volume":"6","author":"MRA Azeem","year":"2014","journal-title":"Archives of Agronomy and Soil Science"},{"key":"ref20","first-page":"44","article-title":"Recombinant D. radiodurans cells for bioremediation of heavy metals from acidic\/neutral aqueous wastes","volume":"3","author":"CS Misra","year":"2012","journal-title":"Bioeng Bugs"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/0304-4165(91)90090-4","article-title":"<italic>Penicillium chrysogenum<\/italic> extracellular acid phosphatase: purification and biochemical characterization","volume":"1074","author":"H Haas","year":"1991","journal-title":"Biochim Biophys Acta"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1515\/znc-2003-3-417","article-title":"Acid phosphatase distribution and localization in the fungus <italic>Humicola lutes<\/italic>","volume":"58","author":"P Aleksieva","year":"2003","journal-title":"Z Naturforsch C"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.jbiotec.2007.08.028","article-title":"Purification and characterization of an acid phosphatase with phytase activity from <italic>Mucor hiemalis<\/italic>","volume":"132","author":"A Boyce","year":"2007","journal-title":"J Biotechnol"},{"issue":"8","key":"ref24","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1271\/bbb.60136","article-title":"Purification and characterization of a novel thermostable extracellular protein tyrosine phosphatase from <italic>Metarhizium anisopliae<\/italic> strain CQMa102","volume":"70","author":"Z Li","year":"2006","journal-title":"Biosci Biotechnol Biochem"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1007\/s10529-010-0264-2","article-title":"Purification and characterization of an acid phosphatase from <italic>Trichoderma harzianum<\/italic>","volume":"32","author":"VO Leit\u00e3o","year":"2010","journal-title":"Biotechnol Lett"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"291","DOI":"10.3923\/ajbs.2011.291.299","article-title":"Plant growth promoting activities of fungi and their effect on Chickpea plant growth","volume":"4","author":"J Yadav","year":"2011","journal-title":"Asian J Biol Sci"},{"key":"ref27","first-page":"19","article-title":"Microscopic and transcriptome analyses of early colonization of tomato roots by <italic>Trichoderma harzianum<\/italic>","volume":"10","author":"MR Chac\u00f3n","year":"2007","journal-title":"Int Microbiol"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/nrmicro797","article-title":"<italic>Trichoderma<\/italic> species- opportunistic, avirulent plant symbionts","volume":"2","author":"GE Harman","year":"2004","journal-title":"Nat Rev Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bej.2007.05.012","article-title":"Antagonistic fungi, <italic>Trichoderma<\/italic> spp.: Panoply of biological control","volume":"37","author":"M Verma","year":"2007","journal-title":"Biochem Eng J"},{"key":"ref30","article-title":"Secretome analysis of the mycoparasitic fungus <italic>Trichoderma harzianum<\/italic> ALL 42 cultivated in different media supplemented with <italic>Fusarium solani<\/italic> cell wall or glucose","author":"MH Ramada","year":"2015","journal-title":"Proteomics"},{"key":"ref31","article-title":"The Cerato-Platanin protein Epl-1 from <italic>Trichoderma harzianum<\/italic> is involved in mycoparasitism, plant resistance induction and self cell wall protection","author":"EV Gomes","year":"2015","journal-title":"Scientific Reports"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1139\/m96-133","article-title":"Purification and characterization of an endo-\u03b2-1,3- glucanase from <italic>Trichoderma harzianum<\/italic>","volume":"42","author":"EF Noronha","year":"1996","journal-title":"Can J Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/BF02092032","article-title":"Purification and characterization of an extracellular chitobiase from <italic>Trichoderma harzianum<\/italic>","volume":"23","author":"CJ Ulhoa","year":"1992","journal-title":"Curr Microbiol"},{"key":"ref34","first-page":"32","article-title":"New cellulase processes for the textile industry. EU\u2014project report","volume":"22","author":"J Buchert","year":"1998","journal-title":"Carbohydr Eur"},{"issue":"4","key":"ref35","first-page":"398","article-title":"Purification, Characterization, and Synergistic Activity of a Glucan 1,3-Glucosidase and an N-Acetyl-Glucosaminidase from <italic>Trichoderma harzianum<\/italic>","volume":"84","author":"M Lorito","year":"1994","journal-title":"Mol Plant Pathol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0076-6879(66)08014-5","article-title":"Assay of inorganic phosphate and phosphatases","volume":"8","author":"BN Ames","year":"1966","journal-title":"Methods Enzymol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Analytical Biochemistry"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during assembly of the head of the bacteriophage T<sub>4<\/sub>","volume":"227","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"issue":"5","key":"ref39","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1021\/ac950914h","article-title":"Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels","volume":"68","author":"A Shevchenko","year":"1996","journal-title":"Anal Chem"},{"key":"ref40","first-page":"571","article-title":"The Proteomics Protocols Handbook","author":"E Gasteiger","year":"2005"},{"issue":"10","key":"ref41","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.2527\/1998.76102649x","article-title":"Adding wheat middlings, microbial phytase, and citric acid to corn-soybean meal diets for growing pigs may replace inorganic phosphorus supplementation","volume":"76","author":"YM Han","year":"1998","journal-title":"J Anim Sci"},{"key":"ref42","doi-asserted-by":"crossref","DOI":"10.1007\/978-0-387-46312-4","article-title":"Principles of Fluorescence Spectroscopy","author":"JR Lakowicz","year":"2006"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1021\/jp8092012","article-title":"Structural Analysis of Human Serum Albumin Complexes with Cationic Lipids","volume":"113","author":"D Charbonneau","year":"2009","journal-title":"J. Phys. Chem. B"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/BF00384615","article-title":"Regulation of synthesis and secretion of acid and alkaline phosphatases in <italic>Neurospora crassa<\/italic>","volume":"11","author":"SW Han","year":"1987","journal-title":"Curr Genet"},{"issue":"2","key":"ref45","doi-asserted-by":"crossref","first-page":"315","DOI":"10.5251\/abjna.2011.2.2.315.324","article-title":"Mycorrhizosphere development and management: The role of nutrients, microorganisms and biochemical activities","volume":"2","author":"KK Dubey","year":"2011","journal-title":"Agric Biol J N Am"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.cca.2011.01.035","article-title":"Serum tartrate-resistant acid phosphatase isoform 5a (TRACP5a) as a potential risk marker in cardiovascular disease","volume":"412","author":"AJ Janckila","year":"2011","journal-title":"Clin Chim Acta"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.bbamcr.2010.01.021","article-title":"Biogenesis of tartrate-resistant acid phosphatase isoforms 5a and 5b in stably transfected MDA-MB-231 breast cancer epithelial cells","volume":"1803","author":"S Zenger","year":"2010","journal-title":"Biochim Biophys Acta"},{"issue":"11","key":"ref48","doi-asserted-by":"crossref","first-page":"5283","DOI":"10.2527\/jas.2015-9317","article-title":"The addition of a sp. Phytase to lactating sow diets deficient in phosphorus and calcium reduces weight loss and improves nutrient digestibility","volume":"93","author":"AL Wealleans","year":"2015","journal-title":"J Anim Sci"},{"key":"ref49","first-page":"2017","article-title":"Effect of carbon source and pH on the production and secretion of acid phosphatase (EC 3.1.3.2.) and alkaline phosphatase (EC 3.1.3.1.) in Neurospora crassa","volume":"128","author":"E Nahas","year":"1982","journal-title":"J Gen Microbiol"},{"key":"ref50","first-page":"423","article-title":"Acid phosphatase (E C 3.1.3.2) synthesis by phosphorus regulatory mutant strain of <italic>Neurospora crassa<\/italic>","volume":"22","author":"SW Han","year":"1989","journal-title":"Braz J Med Biol Res"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1007\/s00284-010-9611-8","article-title":"New Insights in <italic>Trichoderma harzianum<\/italic> Antagonism of Fungal Plant Pathogens by Secreted Protein Analysis","volume":"61","author":"VN Monteiro","year":"2010","journal-title":"Curr Microbiol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/j.funbio.2012.04.015","article-title":"Biochemical and metabolic profiles of <italic>Trichoderma<\/italic> strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against <italic>Sclerotinia sclerotiorum<\/italic>","volume":"11","author":"FAC Lopes","year":"2012","journal-title":"Fungal biol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1128\/JB.100.3.1161-1165.1969","article-title":"Regulation of the formation of acid phosphatases by inorganic phosphate in <italic>Aspergillus ficuum<\/italic>","volume":"100","author":"TR Shieh","year":"1969","journal-title":"J Bacteriol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"4446","DOI":"10.1128\/AEM.64.11.4446-4451.1998","article-title":"Comparison of the thermostability properties of three acid phosphatases from molds: <italic>Aspergillus fumigatus<\/italic> Phytase, <italic>A<\/italic>. <italic>niger<\/italic> Phytase, and <italic>A<\/italic>. <italic>niger<\/italic> pH 2.5 Acid Phosphatase","volume":"64","author":"M Wyss","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"2819","DOI":"10.1099\/00221287-148-9-2819","article-title":"Characterization of a cell-wall acid phosphatase (PhoAp) in <italic>Aspergillus fumigatus<\/italic>","volume":"148","author":"M Bernard","year":"2002","journal-title":"Microbiology"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0141-0229(02)00247-8","article-title":"Effect of culture conditions on the biosynthesis of <italic>Aspergillus niger<\/italic> phytase and acid phosphatase","volume":"32","author":"S Gargova","year":"2003","journal-title":"Enzyme Microb Technol"},{"issue":"4","key":"ref57","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/S1389-1723(03)80066-X","article-title":"Production and properties of phytase and acid phosphatase from sake koji mold, <italic>Aspergillus oryzae<\/italic>","volume":"95","author":"J Fujita","year":"2003","journal-title":"J Biosci Bioeng"},{"issue":"6","key":"ref58","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1016\/S0031-9422(98)00205-2","article-title":"Purification and properties of pi-repressible acid phosphatases from <italic>Aspergillus nidulans<\/italic>","volume":"49","author":"SR Nozawa","year":"1998","journal-title":"Phytochemistry"},{"issue":"1","key":"ref59","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1006\/bbrc.1994.2426","article-title":"An acid phosphatase from <italic>Aspergillus ficuum<\/italic> has homology to <italic>Penicillium chrysogenum<\/italic> PHOA","volume":"204","author":"CK Eherlich","year":"1994","journal-title":"Biochem Biophys Res Commun"},{"issue":"3","key":"ref60","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1023\/A:1002450221778","article-title":"Purification and characterization of an acid phosphatase from the commercial mushroom <italic>Agaricus bisporus<\/italic>","volume":"77","author":"WJ Wannet","year":"2000","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.molcatb.2011.05.010","article-title":"Probing a family GH11 endo-beta-1,4-xylanase inhibition mechanism by phenolic compounds: Role of functional phenolic groups","volume":"72","author":"I Boukari","year":"2011","journal-title":"J Mol Catal B Enzym"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1023\/A:1022398329107","article-title":"Correlating protein structure and protein fluorescence","volume":"13","author":"Y Engelborghs","year":"2003","journal-title":"J Fluoresc"},{"issue":"3","key":"ref63","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/bmb.2002.494030030035","article-title":"Protein tryptophan accessibility studied by fluorescence quenching","volume":"30","author":"M M\u00f6ller","year":"2002","journal-title":"Biochem Mol Biol Educ"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0150455","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T19:21:15Z","timestamp":1718392875000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0150455"}},"subtitle":[],"editor":[{"given":"Claude","family":"Wicker-Thomas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,3,3]]},"references-count":63,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,3,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0150455","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,3]]}}}