{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:40:01Z","timestamp":1791308401410,"version":"4.3.1"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2016,3,17]],"date-time":"2016-03-17T00:00:00Z","timestamp":1458172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0151428","type":"journal-article","created":{"date-parts":[[2016,3,17]],"date-time":"2016-03-17T17:56:31Z","timestamp":1458237391000},"page":"e0151428","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":45,"title":["The Relevance of a Novel Quantitative Assay to Detect up to 40 Major Streptococcus pneumoniae Serotypes Directly in Clinical Nasopharyngeal and Blood Specimens"],"prefix":"10.1371","volume":"11","author":[{"given":"Melina","family":"Messaoudi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milen","family":"Milenkov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner C.","family":"Albrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P. G.","family":"van der Linden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"B\u00e9net","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monidarin","family":"Chou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Sylla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Barreto Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Richard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith P.","family":"Klugman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert P.","family":"Endtz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gl\u00e1ucia","family":"Paranhos-Baccal\u00e0","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-No\u00ebl","family":"Telles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,3,17]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0140-6736(09)61204-6","article-title":"Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates","volume":"374","author":"KL O\u2019Brien","year":"2009","journal-title":"Lancet"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1016\/S0140-6736(10)60549-1","article-title":"Global, regional, and national causes of child mortality in 2008: a systematic analysis","volume":"375","author":"RE Black","year":"2010","journal-title":"Lancet"},{"key":"ref3","first-page":"85","article-title":"Laboratory diagnosis of Streptococcus pneumoniae infections: past and future","volume":"53","author":"R Bandettini","year":"2012","journal-title":"J Prev Med Hyg"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1086\/381547","article-title":"Nasopharyngeal carriage of Streptococcus pneumoniae by adults and children in community and family settings","volume":"38","author":"G Regev-Yochay","year":"2004","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/infdis\/142.6.923","article-title":"Epidemiologic studies of Streptococcus pneumoniae in infants: acquisition, carriage, and infection during the first 24 months of life","volume":"142","author":"BM Gray","year":"1980","journal-title":"J Infect Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1086\/313608","article-title":"Which Pneumococcal Serogroups Cause the Most Invasive Disease: Implications for Conjugate Vaccine Formulation and Use, Part I","volume":"30","author":"WP Hausdorff","year":"2000","journal-title":"Clin Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1097\/00006454-199506000-00007","article-title":"Potential interventions for the prevention of childhood pneumonia: geographic and temporal differences in serotype and serogroup distribution of sterile site pneumococcal isolates from children\u2014implications for vaccine strategies","volume":"14","author":"DH Sniadack","year":"1995","journal-title":"Pediatr Infect Dis J"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1093\/clinids\/22.6.973","article-title":"Serogroup-Specific Epidemiology of Streptococcus pneumoniae: Associations with Age, Sex, and Geography in 7,000 Episodes of Invasive Disease","volume":"22","author":"J a. G Scott","year":"1996","journal-title":"Clin Infect Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1056\/NEJMoa022823","article-title":"Decline in invasive pneumococcal disease after the introduction of protein-polysaccharide conjugate vaccine","volume":"348","author":"CG Whitney","year":"2003","journal-title":"N Engl J Med"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.prrv.2011.12.001","article-title":"Streptococcus pneumoniae\u2013a review of carriage, infection, serotype replacement and vaccination","volume":"13","author":"S Mehr","year":"2012","journal-title":"Paediatr Respir Rev"},{"key":"ref11","first-page":"699","article-title":"The quellung reaction, a neglected microbiologic technique","volume":"43","author":"R Austrian","year":"1976","journal-title":"Mt Sinai J Med N Y"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1469-0691.2011.03496.x","article-title":"Laboratory-based diagnosis of pneumococcal pneumonia: state of the art and unmet needs","volume":"17","author":"G Vernet","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1128\/JCM.42.6.2518-2522.2004","article-title":"Simple, rapid latex agglutination test for serotyping of pneumococci (Pneumotest-Latex)","volume":"42","author":"H-C Slotved","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1128\/JCM.41.6.2378-2384.2003","article-title":"Serotyping Streptococcus pneumoniae by Multiplex PCR","volume":"41","author":"DA Brito","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.1128\/JCM.01061-14","article-title":"Challenges of using molecular serotyping for surveillance of pneumococcal disease","volume":"52","author":"V Magomani","year":"2014","journal-title":"J Clin Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/j.vaccine.2014.01.023","article-title":"Pneumococcal serotype distribution in 1315 nasopharyngeal swabs from a highly vaccinated cohort of Italian children as detected by RT-PCR","volume":"32","author":"A Pasinato","year":"2014","journal-title":"Vaccine"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1086\/656579","article-title":"Community\u2010acquired bacteremic pneumococcal pneumonia in children: diagnosis and serotyping by real\u2010time polymerase chain reaction using blood samples","volume":"51","author":"M Resti","year":"2010","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1542\/peds.76.6.901","article-title":"Routine quantitative blood cultures in children with Haemophilus influenzae or Streptococcus pneumoniae bacteremia","volume":"76","author":"LM Bell","year":"1985","journal-title":"Pediatrics"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1542\/peds.69.6.699","article-title":"Relationship between the magnitude of bacteremia in children and the clinical disease","volume":"69","author":"TD Sullivan","year":"1982","journal-title":"Pediatrics"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/1471-2334-6-79","article-title":"Time to positivity in blood cultures of adults with Streptococcus pneumoniae bacteremia","volume":"6","author":"G Peralta","year":"2006","journal-title":"BMC Infect Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1097\/INF.0b013e3181f111a2","article-title":"Association between nasopharyngeal load of Streptococcus pneumoniae, viral coinfection, and radiologically confirmed pneumonia in Vietnamese children","volume":"30","author":"HTT Vu","year":"2011","journal-title":"Pediatr Infect Dis J"},{"key":"ref22","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2014-005953","article-title":"Pneumococcal colonisation density: a new marker for disease severity in HIV-infected adults with pneumonia","volume":"4","author":"WC Albrich","year":"2014","journal-title":"BMJ Open"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/JCM.44.1.124-131.2006","article-title":"Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates","volume":"44","author":"R Pai","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"4224","DOI":"10.1128\/JCM.01553-14","article-title":"Genomic Load from Sputum Samples and Nasopharyngeal Swabs for Diagnosis of Pneumococcal Pneumonia in HIV-Infected Adults","volume":"52","author":"WC Albrich","year":"2014","journal-title":"J Clin Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-014-0635-8","article-title":"Multicenter case\u2013control study protocol of pneumonia etiology in children: Global Approach to Biological Research, Infectious diseases and Epidemics in Low-income countries (GABRIEL network)","volume":"14","author":"VS Picot","year":"2014","journal-title":"BMC Infect Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e0145447","DOI":"10.1371\/journal.pone.0145447","article-title":"Etiology and Factors Associated with Pneumonia in Children under 5 Years of Age in Mali: A Prospective Case-Control Study","volume":"10","author":"T B\u00e9net","year":"2015","journal-title":"PloS One"},{"key":"ref27","first-page":"11","article-title":"Viral and bacterial pathogens identification in children hospitalised for severe pneumonia and parapneumonic empyema","volume":"1","author":"J-N Telles","year":"2012","journal-title":"Pneumonia Peer Rev Open Access J"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.mimet.2005.11.006","article-title":"Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR","volume":"66","author":"E Reynisson","year":"2006","journal-title":"J Microbiol Methods"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1128\/CDLI.10.4.514-519.2003","article-title":"Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides","volume":"10","author":"CM Wernette","year":"2003","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.femsim.2004.03.013","article-title":"Colony blot assay: a useful method to detect multiple pneumococcal serotypes within clinical specimens","volume":"41","author":"D Bogaert","year":"2004","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1128\/JCM.35.3.764-766.1997","article-title":"Dot blot assay for the serotyping of pneumococci","volume":"35","author":"A Fenoll","year":"1997","journal-title":"J Clin Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1128\/JCM.42.4.1596-1600.2004","article-title":"Immunoblot method to detect Streptococcus pneumoniae and identify multiple serotypes from nasopharyngeal secretions","volume":"42","author":"MA Bronsdon","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1128\/JCM.00157-11","article-title":"Improved detection of nasopharyngeal cocolonization by multiple pneumococcal serotypes by use of latex agglutination or molecular serotyping by microarray","volume":"49","author":"P Turner","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/978-1-61779-373-8_24","article-title":"Measurement of antibodies to pneumococcal polysaccharides with Luminex xMAP microsphere-based liquid arrays","volume":"808","author":"JW Pickering","year":"2012","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1128\/JCM.02927-12","article-title":"Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden","volume":"51","author":"FC Pimenta","year":"2013","journal-title":"J Clin Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"7841","DOI":"10.1128\/JB.00836-07","article-title":"Genetic relatedness of the Streptococcus pneumoniae capsular biosynthetic loci","volume":"189","author":"A Mavroidi","year":"2007","journal-title":"J Bacteriol"},{"key":"ref37","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.0020031","article-title":"Genetic Analysis of the Capsular Biosynthetic Locus from All 90 Pneumococcal Serotypes","volume":"2","author":"SD Bentley","year":"2006","journal-title":"PLoS Genet"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1128\/JCM.33.10.2759-2762.1995","article-title":"Six newly recognized types of Streptococcus pneumoniae","volume":"33","author":"J Henrichsen","year":"1995","journal-title":"J Clin Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"3411","DOI":"10.1128\/JCM.01076-07","article-title":"Identification of Less-Common Streptococcus pneumoniae Serotypes by a Multiplex PCR-Based Reverse Line Blot Hybridization Assay","volume":"45","author":"F Zhou","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"3451","DOI":"10.1128\/JCM.01368-12","article-title":"Rapid and Easy Identification of Capsular Serotypes of Streptococcus pneumoniae by Use of Fragment Analysis by Automated Fluorescence-Based Capillary Electrophoresis","volume":"50","author":"L Selva","year":"2012","journal-title":"J Clin Microbiol"},{"key":"ref41","article-title":"Invasive pneumococcal disease: Clinical outcomes and patient characteristics 2\u20136 years after introduction of 7-valent pneumococcal conjugate vaccine compared to the pre-vaccine period, the Netherlands","author":"GHJ Wagenvoort","year":"2016","journal-title":"Vaccine"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.vaccine.2015.12.056","article-title":"The effect of pneumococcal conjugate vaccines on the incidence of invasive pneumococcal disease caused by ten non-vaccine serotypes in Denmark","volume":"34","author":"H-C Slotved","year":"2016","journal-title":"Vaccine"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.vaccine.2015.12.010","article-title":"Early Streptococcus pneumoniae serotype changes in Utah adults after the introduction of PCV13 in children","volume":"34","author":"BA Kendall","year":"2016","journal-title":"Vaccine"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.vaccine.2015.11.060","article-title":"Nasopharyngeal carriage of Streptococcus pneumoniae and other bacteria in the 7th year after implementation of the pneumococcal conjugate vaccine in the Netherlands","volume":"34","author":"AATM Bosch","year":"2016","journal-title":"Vaccine"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.vaccine.2015.12.042","article-title":"Nasopharyngeal carriage of Streptococcus pneumoniae among children in an urban setting in Brazil prior to PCV10 introduction","volume":"34","author":"O Menezes AP de","year":"2016","journal-title":"Vaccine"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1128\/JCM.39.2.485-493.2001","article-title":"Purification and Characterization of PCR-Inhibitory Components in Blood Cells","volume":"39","author":"WA Al-Soud","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1093\/aje\/154.1.85","article-title":"Interpreting results from trials of pneumococcal conjugate vaccines: a statistical test for detecting vaccine-induced increases in carriage of nonvaccine serotypes","volume":"154","author":"M Lipsitch","year":"2001","journal-title":"Am J Epidemiol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1016\/S0140-6736(03)13772-5","article-title":"Effect of conjugate pneumococcal vaccine followed by polysaccharide pneumococcal vaccine on recurrent acute otitis media: a randomised study","volume":"361","author":"R Veenhoven","year":"2003","journal-title":"Lancet"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1016\/S0140-6736(00)02779-3","article-title":"Prevention of pneumococcal disease by vaccination: does serotype replacement matter?","volume":"356","author":"BG Spratt","year":"2000","journal-title":"Lancet"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1097\/INF.0b013e3182002d14","article-title":"Molecular analysis improves pathogen identification and epidemiologic study of pediatric parapneumonic empyema","volume":"30","author":"AJ Blaschke","year":"2011","journal-title":"Pediatr Infect Dis J"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0151428","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T11:25:07Z","timestamp":1655292307000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0151428"}},"subtitle":[],"editor":[{"given":"Herminia","family":"de Lencastre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,3,17]]},"references-count":50,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2016,3,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0151428","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,17]]}}}