{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T09:06:02Z","timestamp":1773911162048,"version":"3.50.1"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2016,4,7]],"date-time":"2016-04-07T00:00:00Z","timestamp":1459987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0153183","type":"journal-article","created":{"date-parts":[[2016,4,7]],"date-time":"2016-04-07T18:54:57Z","timestamp":1460055297000},"page":"e0153183","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Influenza Neuraminidase Subtype N1: Immunobiological Properties and Functional Assays for Specific Antibody Response"],"prefix":"10.1371","volume":"11","author":[{"given":"Don","family":"Changsom","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatairat","family":"Lerdsamran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Witthawat","family":"Wiriyarat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warunya","family":"Chakritbudsabong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bunpote","family":"Siridechadilok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jarunee","family":"Prasertsopon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pirom","family":"Noisumdaeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanibtisam","family":"Masamae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilaipan","family":"Puthavathana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,4,7]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1146\/annurev.iy.08.040190.003513","article-title":"Structural basis of immune recognition of influenza virus hemagglutinin","volume":"8","author":"IA Wilson","year":"1990","journal-title":"Annu Rev Immunol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/0042-6822(74)90276-1","article-title":"Characterization of temperature-sensitive influenza virus mutants defective in neuraminidase","volume":"61","author":"P Palese","year":"1974","journal-title":"Virology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1016\/j.micinf.2005.12.008","article-title":"Roles of neuraminidase in the initial stage of influenza virus infection","volume":"8","author":"M Ohuchi","year":"2006","journal-title":"Microbes Infect"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"12665","DOI":"10.1128\/JVI.78.22.12665-12667.2004","article-title":"Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium","volume":"78","author":"MN Matrosovich","year":"2004","journal-title":"J Virol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1186\/1743-422X-10-321","article-title":"Influenza A penetrates host mucus by cleaving sialic acids with neuraminidase","volume":"10","author":"M Cohen","year":"2013","journal-title":"Virol J"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"6869","DOI":"10.1073\/pnas.84.19.6869","article-title":"Antigen-presenting B cells and helper T cells cooperatively mediate intravirionic antigenic competition between influenza A virus surface glycoproteins","volume":"84","author":"BE Johansson","year":"1987","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref7","first-page":"647","article-title":"The role of antineuraminidase antibody in immunity to influenza virus infection","volume":"41","author":"JL Schulman","year":"1969","journal-title":"Bull World Health Organ"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/S0140-6736(73)92196-X","article-title":"Effect of neuraminidase antibody on Hong Kong influenza","volume":"1","author":"AS Monto","year":"1973","journal-title":"Lancet"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e59","DOI":"10.1371\/journal.pmed.0040059","article-title":"Cross-reactive neuraminidase antibodies afford partial protection against H5N1 in mice and are present in unexposed humans","volume":"4","author":"MR Sandbulte","year":"2007","journal-title":"PLoS MED"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3763","DOI":"10.1016\/j.vaccine.2007.02.011","article-title":"Influenza neuraminidase antibodies provide partial protection for chickens against high pathogenic avian influenza infection","volume":"25","author":"MJ Sylte","year":"2007","journal-title":"Vaccine"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"3214","DOI":"10.1016\/S0264-410X(00)00149-3","article-title":"Cross-protection against a lethal influenza virus infection by DNA vaccine to neuraminidase","volume":"18","author":"Z Chen","year":"2000","journal-title":"Vaccine"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2516","DOI":"10.1016\/j.vaccine.2012.01.090","article-title":"The 2009 pandemic H1N1 virus induces anti-neuraminidase (NA) antibodies that cross-react with the NA of H5N1 viruses in ferrets","volume":"30","author":"Z Chen","year":"2012","journal-title":"Vaccine"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1128\/JVI.02434-12","article-title":"Neuraminidase-inhibiting antibody is a correlate of cross-protection against lethal H5N1 influenza virus in ferrets immunized with seasonal influenza vaccine","volume":"87","author":"S Rockman","year":"2013","journal-title":"J Virol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1111\/j.1432-1033.1997.00332.x","article-title":"Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidase","volume":"247","author":"W Martinet","year":"1997","journal-title":"Eur J Biochem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s11033-011-0809-z","article-title":"Expression, purification and characterization of low-glycosylation influenza neuraminidase in \u03b1-1,6-mannosyltransferase defective Pichia pastoris","volume":"39","author":"YL Yang","year":"2012","journal-title":"Mol Biol Rep"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/0042-6822(88)90640-X","article-title":"Protection against lethal influenza with neuraminidase","volume":"164","author":"RG Webster","year":"1988","journal-title":"Virology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1099\/0022-1317-70-6-1523","article-title":"Mechanism of immunity to influenza: maternal and passive neonatal protection following immunization of adult ferrets with a live vaccinia-influenza virus haemagglutinin recombinant but not with recombinants containing other influenza virus proteins","volume":"70","author":"KJ Jakeman","year":"1989","journal-title":"J Gen Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"5484","DOI":"10.4049\/jimmunol.150.12.5484","article-title":"Beta 2-microglobulin-deficient mice can be protected against influenza A infection by vaccination with vaccinia-influenza recombinants expressing hemagglutinin and neuraminidase","volume":"150","author":"SL Epstein","year":"1993","journal-title":"J Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e12217","DOI":"10.1371\/journal.pone.0012217","article-title":"A pandemic influenza H1N1 live vaccine based on modified vaccinia ankara is highly immunogenic and protects mice in active and passive immunizations","volume":"5","author":"A Hessel","year":"2010","journal-title":"PLoS ONE"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"e02556","DOI":"10.1128\/mBio.02556-14","article-title":"Vaccination with adjuvanted recombinant neuraminidase induces broad heterologous, but not heterosubtypic, cross-protection against influenza virus infection in mice","volume":"6","author":"TJ Wohlbold","year":"2015","journal-title":"MBio"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0264-410X(94)00071-T","article-title":"Immunogenicity of influenza A virus N2 neuraminidase produced in insect larvae by baculovirus recombinants","volume":"13","author":"BE Johansson","year":"1995","journal-title":"Vaccine"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/0264-410X(95)00157-V","article-title":"Recombinant neuraminidase vaccine protects against lethal Influenza","volume":"14","author":"T Deroo","year":"1996","journal-title":"Vaccine"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1086\/381123","article-title":"Protection of mice with recombinant influenza virus neuraminidase","volume":"189","author":"ED Kilbourne","year":"2004","journal-title":"J Infect Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.virol.2012.06.003","article-title":"Protection against a lethal H5N1 influenza challenge by intranasal immunization with virus-like particles containing 2009 pandemic H1N1 neuraminidase in mice","volume":"432","author":"JD Easterbrook","year":"2012","journal-title":"Virology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.virol.2012.05.006","article-title":"Influenza M1 VLPs containing neuraminidase induce heterosubtypic cross-protection","volume":"430","author":"FS Quan","year":"2012","journal-title":"Virology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1016\/j.vaccine.2008.06.012","article-title":"FDA\/NIH\/WHO public workshop on immune correlates of protection against influenza A viruses in support of pandemic vaccine development, Bethesda, Maryland, US, December 10\u201311, 2007","volume":"26","author":"M Eichelberger","year":"2008","journal-title":"Vaccine"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1006\/viro.2002.1625","article-title":"Mechanism of neutralization of influenza virus infectivity by antibodies","volume":"25","author":"M Knossow","year":"2002","journal-title":"Virology"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1750-2659.2009.00094.x","article-title":"A miniaturized assay for influenza neuraminidase-inhibiting antibodies utilizing reverse genetics derived antigens","volume":"3","author":"MR Sandbulte","year":"2009","journal-title":"Influenza Other Respir Viruses"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.1016\/j.vaccine.2009.12.041","article-title":"A high dosage influenza vaccine induced significantly more neuraminidase antibody than standard vaccine among elderly subjects","volume":"28","author":"TR Cate","year":"2010","journal-title":"Vaccine"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e26335","DOI":"10.1371\/journal.pone.0026335","article-title":"A contributing role for anti-neuraminidase antibodies on immunity to pandemic H1N1 2009 influenza A virus","volume":"6","author":"G Marcelin","year":"2011","journal-title":"PLoS ONE"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"9290","DOI":"10.1128\/JVI.01203-13","article-title":"Molecular basis for broad neuraminidase immunity: conserved epitopes in seasonal and pandemic H1N1 as well as H5N1 influenza viruses","volume":"87","author":"H Wan","year":"2013","journal-title":"J Virol"},{"issue":"5","key":"ref32","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1128\/JB.92.5.1521-1534.1966","article-title":"Reduction in plaque size and reduction in plaque number as differing indices of influenza virus-antibody reactions","volume":"92","author":"RI Jahiel","year":"1966","journal-title":"J Bacteriol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1128\/JVI.2.4.281-288.1968","article-title":"Antiviral activity of antiserum specific for an influenza virus neuraminidase","volume":"2","author":"ED Kilbourne","year":"1968","journal-title":"J Virol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.virol.2014.02.024","article-title":"Homosubtypic and heterosubtypic antibodies against highly pathogenic avian influenza H5N1 recombinant proteins in H5N1 survivors and non-H5N1 subjects","volume":"454\u2013455","author":"P Noisumdaeng","year":"2014","journal-title":"Virology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1007\/s007050170002","article-title":"Universal primer set for the full-length amplification of all influenza A viruses","volume":"146","author":"E Hoffmann","year":"2001","journal-title":"Arch Virol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1128\/MCB.5.12.3403","article-title":"Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques","volume":"5","author":"S Chakrabarti","year":"1985","journal-title":"Mol Cell Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"6108","DOI":"10.1073\/pnas.100133697","article-title":"A DNA transfection system for generation of influenza A virus from eight plasmids","volume":"97","author":"E Hoffmann","year":"2000","journal-title":"Proc Natl AcadSci U S A"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jviromet.2014.09.003","article-title":"An optimized enzyme-linked lectin assay to measure influenza A virus neuraminidase inhibition antibody titers in human sera","volume":"210","author":"L Couzens","year":"2014","journal-title":"J Virol Methods"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"e16164","DOI":"10.1371\/journal.pone.0016164","article-title":"Serological response to the 2009 pandemic influenza A (H1N1) virus for disease diagnosis and estimating the infection rate in Thai population","volume":"6","author":"H Lerdsamran","year":"2011","journal-title":"PLoS ONE"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e36318","DOI":"10.1371\/journal.pone.0036318","article-title":"A novel pathogenic mechanism of highly pathogenic avian influenza H5N1 viruses involves hemagglutinin mediated resistance to serum innate inhibitors","volume":"7","author":"J Panaampon","year":"2012","journal-title":"PLoS ONE"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1042\/bj2090331","article-title":"Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes","volume":"209","author":"E Bause","year":"1983","journal-title":"Biochem J"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014a visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/0042-6822(87)90004-3","article-title":"Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses","volume":"160","author":"GL Smith","year":"1987","journal-title":"Virology"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jviromet.2008.10.025","article-title":"Avian influenza A\/H5N1 neuraminidase expressed in yeast with a functional head domain","volume":"156","author":"S Yongkiettrakul","year":"2009","journal-title":"J Virol Methods"},{"issue":"4","key":"ref45","doi-asserted-by":"crossref","first-page":"e95539","DOI":"10.1371\/journal.pone.0095539","article-title":"A 20-amino-acid deletion in the neuraminidase stalk and a five-amino-acid deletion in the NS1 protein both contribute to the pathogenicity of H5N1 avian influenza viruses in mallard ducks","volume":"9","author":"Y Li","year":"2014","journal-title":"PLoS ONE"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1128\/JVI.02949-14","article-title":"Biological and protective properties of immune sera directed to the influenza virus neuraminidase","volume":"89","author":"SJ Halbherr","year":"2015","journal-title":"J Virol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"10366","DOI":"10.1128\/JVI.01035-10","article-title":"Recombinant soluble multimeric HA and NA exhibit distinctive types of protection against pandemic swine-origin 2009 A (H1N1) influenza virus infection in ferrets","volume":"84","author":"BJ Bosch","year":"2010","journal-title":"J Virol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"e42363","DOI":"10.1371\/journal.pone.0042363","article-title":"A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus","volume":"7","author":"CY Wu","year":"2012","journal-title":"PLoS ONE"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1038\/nature08182","article-title":"Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic","volume":"459","author":"GJ Smith","year":"2009","journal-title":"Nature"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1128\/JVI.63.3.1239-1246.1989","article-title":"Purified influenza virus hemagglutinin and neuraminidase are equivalent in stimulation of antibody response but induce contrasting types of immunity to infection","volume":"63","author":"BE Johansson","year":"1989","journal-title":"J Virol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.vaccine.2012.10.065","article-title":"Randomized comparative study of the serum antihemagglutinin and antineuraminidase antibody responses to six licensed trivalent influenza vaccines","volume":"31","author":"RB Couch","year":"2012","journal-title":"Vaccine"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"4598","DOI":"10.1016\/j.vaccine.2005.04.042","article-title":"Estimation of the neuraminidase content of influenza viruses and split-product vaccines by immunochromatography","volume":"23","author":"T Tanimoto","year":"2005","journal-title":"Vaccine"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"6366","DOI":"10.1016\/j.vaccine.2009.02.084","article-title":"WHO meeting on the role of neuraminidase in inducing protective immunity against influenza infection, Vilamoura, Portugal, September 14, 2008","volume":"27","author":"RA Bright","year":"2009","journal-title":"Vaccine"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/S0264-410X(98)00247-3","article-title":"Enhanced protection against a lethal influenza virus challenge by immunization with both hemagglutinin and neuraminidase expressing DNAs","volume":"17","author":"Z Chen","year":"1999","journal-title":"Vaccine"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1016\/S0264-410X(98)00413-7","article-title":"Immunization with influenza A virus hemagglutinin and neuraminidase produced in recombinant baculovirus results in a balanced and broadened immune response superior to conventional vaccine","volume":"17","author":"BE Johansson","year":"1999","journal-title":"Vaccine"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0153183","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T15:28:56Z","timestamp":1718465336000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0153183"}},"subtitle":[],"editor":[{"given":"Stephen Mark","family":"Tompkins","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,4,7]]},"references-count":55,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,4,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0153183","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,7]]}}}