{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T22:04:37Z","timestamp":1770415477471,"version":"3.49.0"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T00:00:00Z","timestamp":1463011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007137","name":"Helse Nord RHF","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007137","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0155101","type":"journal-article","created":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T17:59:56Z","timestamp":1463075996000},"page":"e0155101","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":47,"title":["The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification (LAMP) Assay for Tuberculosis Diagnosis in Adults with Chronic Cough in Malawi"],"prefix":"10.1371","volume":"11","author":[{"given":"Marriott","family":"Nliwasa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"MacPherson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Palesa","family":"Chisala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercy","family":"Kamdolozi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"McEwen","family":"Khundi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kruger","family":"Kaswaswa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mphatso","family":"Mwapasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chisomo","family":"Msefula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hojoon","family":"Sohn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clare","family":"Flach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth L.","family":"Corbett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,5,12]]},"reference":[{"key":"ref1","unstructured":"World Health Organisation. End TB Strategy: Global strategy and targets for tuberculosis prevention, care and control after 2015 2015 [cited 2015 14 October 2015]. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/tb\/post2015_strategy\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/tb\/post2015_strategy\/en\/<\/ext-link>."},{"issue":"11","key":"ref2","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1056\/NEJMoa0907847","article-title":"Rapid molecular detection of tuberculosis and rifampin resistance","volume":"363","author":"CC Boehme","year":"2010","journal-title":"New England Journal of Medicine"},{"key":"ref3","article-title":"Global Tuberculosis Report 2015","year":"2015"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.5588\/ijtld.10.0629","article-title":"Evaluation of a simple loop-mediated isothermal amplification test kit for the diagnosis of tuberculosis","volume":"15","author":"S Mitarai","year":"2011","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref5","unstructured":"UNITAID. Tuberculosis diagnostic technology landscape: semi-annual update 2012 [cited 2015 24 August 2015]. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/globalhealthmarketdynamics.wordpress.com\/resources\/tuberculosis-diagnostic-technology-landscape\/\" xlink:type=\"simple\">https:\/\/globalhealthmarketdynamics.wordpress.com\/resources\/tuberculosis-diagnostic-technology-landscape\/<\/ext-link>."},{"key":"ref6","unstructured":"Foundation for innovative new diagnostics. Price for Xpert\u00ae MTB\/RIF and FIND country list 2013 [cited 2015 07 October 2015]. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.finddiagnostics.org\/about\/what_we_do\/successes\/find-negotiated-prices\/xpert_mtb_rif.html\" xlink:type=\"simple\">http:\/\/www.finddiagnostics.org\/about\/what_we_do\/successes\/find-negotiated-prices\/xpert_mtb_rif.html<\/ext-link>."},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jiac.2013.07.003","article-title":"Rapid and effective diagnosis of pulmonary tuberculosis with novel and sensitive loop-mediated isothermal amplification (LAMP) assay in clinical samples: a meta-analysis","volume":"20","author":"LY Yuan","year":"2014","journal-title":"J Infect Chemother"},{"issue":"2","key":"ref8","first-page":"199","article-title":"Using tuberculosis suspects to identify patients eligible for antiretroviral treatment","volume":"10","author":"L Munthali","year":"2006","journal-title":"International Journal of Tuberculosis & Lung Disease"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1365-3156.2011.02946.x","article-title":"Suboptimal patterns of provider initiated HIV testing and counselling, antiretroviral therapy eligibility assessment and referral in primary health clinic attendees in Blantyre","volume":"17","author":"P MacPherson","year":"2012","journal-title":"Malawi. Trop Med Int Health"},{"issue":"9","key":"ref10","doi-asserted-by":"crossref","first-page":"e105428","DOI":"10.1371\/journal.pone.0105428","article-title":"High uptake of systematic HIV counseling and testing and TB symptom screening at a primary care clinic in South Africa","volume":"9","author":"A Van Rie","year":"2014","journal-title":"PLoS One"},{"key":"ref11","unstructured":"World Health Organization. WHO policy on collaborative TB\/HIV activities: Guidelines for national programmes and other stakeholders. 2012."},{"issue":"10","key":"ref12","doi-asserted-by":"crossref","first-page":"e1001102","DOI":"10.1371\/journal.pmed.1001102","article-title":"The uptake and accuracy of oral kits for HIV self-testing in high HIV prevalence setting: a cross-sectional feasibility study in Blantyre, Malawi","volume":"8","author":"AT Choko","year":"2011","journal-title":"PLoS Med"},{"key":"ref13","unstructured":"National Tuberculosis Control programme. Malawi Nationwide Tuberculosis Prevalence Survey. 45<sup>th<\/sup> Union World Conference on Lung Health. Barcelona, Spain: 2014."},{"key":"ref14","unstructured":"Ministry of Health. National tuberculosis control programme manual, 7th edition\u20142012. 2012 [cited 2015 22 March 2015]. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.medcol.mw\/globalhealth\/uploads\/MalawianTBguidelines2012.pdf\" xlink:type=\"simple\">http:\/\/www.medcol.mw\/globalhealth\/uploads\/MalawianTBguidelines2012.pdf<\/ext-link>."},{"key":"ref15","first-page":"2013"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1128\/JCM.02352-06","article-title":"Operational feasibility of using loop-mediated isothermal amplification for diagnosis of pulmonary tuberculosis in microscopy centers of developing countries","volume":"45","author":"CC Boehme","year":"2007","journal-title":"J Clin Microbiol"},{"issue":"5","key":"ref17","doi-asserted-by":"crossref","first-page":"e94544","DOI":"10.1371\/journal.pone.0094544","article-title":"Diagnostic accuracy of the PURE-LAMP test for pulmonary tuberculosis at the county-level laboratory in China","volume":"9","author":"X Ou","year":"2014","journal-title":"PLoS One"},{"issue":"12","key":"ref18","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1086\/429912","article-title":"Chronic cough in primary health care attendees, Harare, Zimbabwe: diagnosis and impact of HIV infection","volume":"40","author":"SS Munyati","year":"2005","journal-title":"Clinical Infectious Diseases"},{"issue":"9","key":"ref19","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1111\/j.1553-2712.1996.tb03538.x","article-title":"Statistical methodology: I. Incorporating the prevalence of disease into the sample size calculation for sensitivity and specificity","volume":"3","author":"NM Buderer","year":"1996","journal-title":"Acad Emerg Med"},{"issue":"2","key":"ref20","first-page":"266","article-title":"Fluorescence microscopy is less expensive than Ziehl-Neelsen microscopy in Thailand","volume":"13","author":"H Sohn","year":"2009","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref21","unstructured":"United Nations. UN Operational Rates of Exchange [cited 2015 14 August 2015]. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/treasury.un.org\/operationalrates\/OperationalRates.aspx\" xlink:type=\"simple\">http:\/\/treasury.un.org\/operationalrates\/OperationalRates.aspx<\/ext-link>."},{"key":"ref22","article-title":"Initiation of Antiretroviral Therapy in Early Asymptomatic HIV Infection","year":"2015","journal-title":"N Engl J Med"},{"issue":"6","key":"ref23","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1128\/JCM.41.6.2616-2622.2003","article-title":"Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples","volume":"41","author":"T Iwamoto","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.jinf.2013.02.005","article-title":"Clinical value of IS6110-based loop-mediated isothermal amplification for detection of Mycobacterium tuberculosis complex in respiratory specimens","volume":"66","author":"E Aryan","year":"2013","journal-title":"J Infect"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.mimet.2010.10.015","article-title":"Efficacy of loop mediated isothermal amplification (LAMP) assay for the laboratory identification of Mycobacterium tuberculosis isolates in a resource limited setting","volume":"84","author":"G Geojith","year":"2011","journal-title":"J Microbiol Methods"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"212","DOI":"10.7883\/yoken.JJID.2009.212","article-title":"Loop-mediated isothermal amplification (LAMP) for the direct detection of human pulmonary infections with environmental (nontuberculosis) mycobacteria","volume":"62","author":"BR Adhikari","year":"2009","journal-title":"Jpn J Infect Dis"},{"key":"ref27","article-title":"Development and clinical evaluation of sdaA loop mediated isothermal amplification assay for the detection of M. tuberculosis with an approach to prevent carry-over contamination","author":"M Nimesh","year":"2014","journal-title":"J Clin Microbiol"},{"key":"ref28","author":"SW Thiong\u2019o","year":"2014"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"126","DOI":"10.2471\/BLT.13.124800","article-title":"Pre-treatment loss to follow-up in tuberculosis patients in low- and lower-middle-income countries and high-burden countries: a systematic review and meta-analysis","volume":"92","author":"P MacPherson","year":"2014","journal-title":"Bull World Health Organ"},{"issue":"9","key":"ref30","first-page":"e1001873","article-title":"Uptake, Accuracy, Safety, and Linkage into Care over Two Years of Promoting Annual Self-Testing for HIV in Blantyre, Malawi: A Community-Based Prospective Study","volume":"12","author":"AT Choko","year":"2015"},{"issue":"9","key":"ref31","doi-asserted-by":"crossref","first-page":"e44794","DOI":"10.1371\/journal.pone.0044794","article-title":"Determinants and consequences of failure of linkage to antiretroviral therapy at primary care level in Blantyre, Malawi: a prospective cohort study","volume":"7","author":"P MacPherson","year":"2012","journal-title":"PLoS One"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"18020","DOI":"10.7448\/IAS.15.2.18020","article-title":"Barriers and facilitators to linkage to ART in primary care: a qualitative study of patients and providers in Blantyre, Malawi","volume":"15","author":"P MacPherson","year":"2012","journal-title":"J Int AIDS Soc"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0155101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T05:46:32Z","timestamp":1692337592000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0155101"}},"subtitle":[],"editor":[{"given":"Delmiro","family":"Fernandez-Reyes","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,5,12]]},"references-count":32,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2016,5,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0155101","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,12]]}}}