{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:41:02Z","timestamp":1789112462033,"version":"build-2803163510"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T00:00:00Z","timestamp":1464048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0156094","type":"journal-article","created":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T17:35:14Z","timestamp":1464111314000},"page":"e0156094","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":31,"title":["Antibody and Cytokine Responses of Koalas (Phascolarctos cinereus) Vaccinated with Recombinant Chlamydial Major Outer Membrane Protein (MOMP) with Two Different Adjuvants"],"prefix":"10.1371","volume":"11","author":[{"given":"Shahneaz Ali","family":"Khan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Desclozeaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"Waugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon","family":"Hanger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo","family":"Loader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Gerdts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Potter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Polkinghorne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Beagley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Timms","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,5,24]]},"reference":[{"key":"ref1","article-title":"Recent advances in understanding the biology, epidemiology and control of chlamydial infections in koalas","author":"A Polkinghorne","year":"2013","journal-title":"Veterinary microbiology"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1071\/WR02029","article-title":"Koala mortality on roads in south-east Queensland: the koala speed-zone trial","volume":"30","author":"DS Dique","year":"2003","journal-title":"Wildlife research"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1071\/PC070189","article-title":"The impact of fire and dogs on Koalas at Port Stephens, New South Wales, using population viability analysis","volume":"13","author":"D Lunney","year":"2007","journal-title":"Pacific conservation biology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1071\/WR9930177","article-title":"Low genetic diversity and inbreeding depression in Queensland Koalas","volume":"20","author":"JW Wilmer","year":"1993","journal-title":"Wildlife research"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1016\/j.biocon.2010.12.027","article-title":"Using integrated population modelling to quantify the implications of multiple threatening processes for a rapidly declining population","volume":"144","author":"JR Rhodes","year":"2011","journal-title":"Biological conservation"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"S88","DOI":"10.1086\/652394","article-title":"<italic>Chlamydia trachomatis<\/italic> persistence in vitro: an overview","volume":"201","author":"PB Wyrick","year":"2010","journal-title":"Journal of infectious diseases"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"S114","DOI":"10.1086\/652397","article-title":"Pathogenesis of genital tract disease due to <italic>Chlamydia trachomatis<\/italic>","volume":"201","author":"T Darville","year":"2010","journal-title":"Journal of infectious diseases"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S1369-5274(02)00291-6","article-title":"T cell responses to <italic>Chlamydia trachomatis<\/italic>","volume":"5","author":"WP Loomis","year":"2002","journal-title":"Current opinion in microbiology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.vaccine.2015.12.050","article-title":"Humoral immune responses in koalas (<italic>Phascolarctos cinereus<\/italic>) either naturally infected with <italic>Chlamydia pecorum<\/italic> or following administration of a recombinant chlamydial major outer membrane protein vaccine","volume":"34","author":"SA Khan","year":"2016","journal-title":"Vaccine"},{"issue":"Suppl 2","key":"ref10","doi-asserted-by":"crossref","first-page":"S178","DOI":"10.1086\/652400","article-title":"Protective immunity to <italic>Chlamydia trachomatis<\/italic> genital infection: evidence from human studies","volume":"201","author":"BE Batteiger","year":"2010","journal-title":"The Journal of infectious diseases"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1155\/1998\/395297","article-title":"T lymphocyte immunity in host defence against <italic>Chlamydia trachomatis<\/italic> and its implication for vaccine development","volume":"9","author":"X Yang","year":"1998","journal-title":"The Canadian journal of infectious diseases"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/IAI.00984-10","article-title":"Interleukin-17 contributes to generation of Th1 immunity and neutrophil recruitment during <italic>Chlamydia muridarum<\/italic> genital tract infection but is not required for macrophage influx or normal resolution of infection","volume":"79","author":"AM Scurlock","year":"2011","journal-title":"Infection and immunity"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e76664","DOI":"10.1371\/journal.pone.0076664","article-title":"The duration of <italic>Chlamydia muridarum<\/italic> genital tract infection and associated chronic pathological changes are reduced in IL-17 knockout mice but protection is not increased further by immunization","volume":"8","author":"DW Andrew","year":"2013","journal-title":"PloS one"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/j.vaccine.2011.12.125","article-title":"Vaccination of healthy and diseased koalas (<italic>Phascolarctos cinereus<\/italic>) with a <italic>Chlamydia pecorum<\/italic> multi-subunit vaccine: evaluation of immunity and pathology","volume":"30","author":"A Kollipara","year":"2012","journal-title":"Vaccine"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/j.vaccine.2012.12.057","article-title":"Antigenic specificity of a monovalent versus polyvalent MOMP based <italic>Chlamydia pecorum<\/italic> vaccine in koalas (<italic>Phascolarctos cinereus<\/italic>)","volume":"31","author":"A Kollipara","year":"2013","journal-title":"Vaccine"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1600-0897.2009.00776.x","article-title":"A multi-subunit chlamydial vaccine induces antibody and cell-mediated immunity in immunized koalas (<italic>Phascolarctos cinereus<\/italic>): comparison of three different adjuvants","volume":"63","author":"AJ Carey","year":"2010","journal-title":"American journal of reproductive immunology (New York, NY: 1989)"},{"key":"ref17","article-title":"Comparison of subcutaneous versus intranasal immunization of male koalas (<italic>Phascolarctos cinereus<\/italic>) for induction of mucosal and systemic immunity against <italic>Chlamydia pecorum<\/italic>","author":"CA Waugh","year":"2015","journal-title":"Vaccine"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"5781","DOI":"10.1016\/j.vaccine.2014.08.037","article-title":"Vaccination of koalas (<italic>Phascolarctos cinereus<\/italic>) with a recombinant chlamydial major outer membrane protein adjuvanted with poly I:C, a host defense peptide and polyphosphazine, elicits strong and long lasting cellular and humoral immune responses","volume":"32","author":"SA Khan","year":"2014","journal-title":"Vaccine"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/j.vaccine.2006.10.011","article-title":"Poly [di (sodium carboxylatoethylphenoxy) phosphazene](PCEP) is a potent enhancer of mixed Th1\/Th2 immune responses in mice immunized with influenza virus antigens","volume":"25","author":"G Mutwiri","year":"2007","journal-title":"Vaccine"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.vaccine.2011.10.086","article-title":"Comparison of immune responses and protective efficacy of intranasal prime-boost immunization regimens using adenovirus-based and CpG\/HH2 adjuvanted-subunit vaccines against genital Chlamydia muridarum infection","volume":"30","author":"TH Brown","year":"2012","journal-title":"Vaccine"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"2910","DOI":"10.1128\/JVI.79.5.2910-2919.2005","article-title":"Synthetic double-stranded RNA poly (I: C) combined with mucosal vaccine protects against influenza virus infection","volume":"79","author":"T Ichinohe","year":"2005","journal-title":"Journal of virology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e1000373","DOI":"10.1371\/journal.ppat.1000373","article-title":"Synthetic double-stranded RNAs are adjuvants for the induction of T helper 1 and humoral immune responses to human papillomavirus in <italic>rhesus macaques<\/italic>","volume":"5","author":"C Stahl-Hennig","year":"2009","journal-title":"PLoS pathogens"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.vetimm.2012.01.021","article-title":"Administration of poly [di (sodium carboxylatoethylphenoxy)] phosphazene (PCEP) as adjuvant activated mixed Th1\/Th2 immune responses in pigs","volume":"146","author":"A Dar","year":"2012","journal-title":"Veterinary immunology and immunopathology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e0146934","DOI":"10.1371\/journal.pone.0146934","article-title":"A Prototype Recombinant-Protein Based <italic>Chlamydia pecorum<\/italic> Vaccine Results in Reduced Chlamydial Burden and Less Clinical Disease in Free-Ranging Koalas (<italic>Phascolarctos cinereus<\/italic>)","volume":"11","author":"C Waugh","year":"2016","journal-title":"PloS one"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e59958","DOI":"10.1371\/journal.pone.0059958","article-title":"Preliminary Characterisation of Tumor Necrosis Factor Alpha and Interleukin-10 Responses to <italic>Chlamydia pecorum<\/italic> Infection in the Koala (<italic>Phascolarctos cinereus<\/italic>)","volume":"8","author":"M Mathew","year":"2013","journal-title":"PloS one"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.gene.2013.06.019","article-title":"Molecular characterisation and expression analysis of Interferon gamma in response to natural <italic>Chlamydia<\/italic> infection in the koala, <italic>Phascolarctos cinereus<\/italic>","volume":"527","author":"M Mathew","year":"2013","journal-title":"Gene"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.dci.2014.05.015","article-title":"Interleukin 17A is an immune marker for chlamydial disease severity and pathogenesis in the koala (<italic>Phascolarctos cinereus<\/italic>)","volume":"46","author":"M Mathew","year":"2014","journal-title":"Developmental and comparative immunology"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212 \u0394\u0394CT method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"methods"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1128\/IAI.65.3.1032-1044.1997","article-title":"Genital tract infection with <italic>Chlamydia trachomatis<\/italic> fails to induce protective immunity in gamma interferon receptor-deficient mice despite a strong local immunoglobulin A response","volume":"65","author":"M Johansson","year":"1997","journal-title":"Infection and immunity"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1071\/ZO12013","article-title":"Diversity of MHC class II DAB1 in the koala (<italic>Phascolarctos cinereus<\/italic>)","volume":"60","author":"SE Jobbins","year":"2012","journal-title":"Australian journal of zoology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/hdy.2014.30","article-title":"MHC class II diversity of koala (<italic>Phascolarctos cinereus<\/italic>) populations across their range","volume":"113","author":"Q Lau","year":"2014","journal-title":"Heredity"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"5886","DOI":"10.4049\/jimmunol.0901584","article-title":"IL-17\/Th17 promotes type 1 T cell immunity against pulmonary intracellular bacterial infection through modulating dendritic cell function","volume":"183","author":"H Bai","year":"2009","journal-title":"The journal of immunology"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.micinf.2010.10.012","article-title":"Spontaneous secretion of interleukin-17 and-22 by human cervical cells in <italic>Chlamydia trachomatis<\/italic> infection","volume":"13","author":"R Jha","year":"2011","journal-title":"Microbes and infection"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/icb.2013.92","article-title":"Immunity against a <italic>Chlamydia<\/italic> infection and disease may be determined by a balance of IL-17 signaling","volume":"92","author":"CP O'Meara","year":"2014","journal-title":"Immunology and cell biology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"4212","DOI":"10.4049\/jimmunol.164.8.4212","article-title":"Suppression of endogenous IL-10 gene expression in dendritic cells enhances antigen presentation for specific Th1 induction: potential for cellular vaccine development","volume":"164","author":"JU Igietseme","year":"2000","journal-title":"The journal of immunology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.4049\/jimmunol.162.2.1010","article-title":"IL-10 gene knockout mice show enhanced Th1-like protective immunity and absent granuloma formation following <italic>Chlamydia trachomatis<\/italic> lung infection","volume":"162","author":"X Yang","year":"1999","journal-title":"The journal of immunology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1086\/315891","article-title":"Polymorphisms in candidate genes and risk of scarring trachoma in a <italic>Chlamydia trachomatis<\/italic>\u2014endemic population","volume":"182","author":"N Mozzato-Chamay","year":"2000","journal-title":"Journal of infectious diseases"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1093\/infdis\/168.6.1528","article-title":"Conjunctival scarring in trachoma is associated with depressed cell-mediated immune responses to chlamydial antigens","volume":"168","author":"MJ Holland","year":"1993","journal-title":"Journal of infectious diseases"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.clim.2008.10.004","article-title":"Protective or pathogenic immune response to genital chlamydial infection in women\u2014a possible role of cytokine secretion profile of cervical mucosal cells","volume":"130","author":"T Agrawal","year":"2009","journal-title":"Clinical immunology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/1477-7827-7-38","article-title":"Host immune responses to chlamydial inclusion membrane proteins B and C in <italic>Chlamydia trachomatis<\/italic> infected women with or without fertility disorders","volume":"7","author":"R Gupta","year":"2009","journal-title":"Reproductive biology and endocrinology"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1086\/597620","article-title":"Cytokine polymorphisms and severity of tubal damage in women with <italic>Chlamydia<\/italic>-associated infertility","volume":"199","author":"H \u00d6hman","year":"2009","journal-title":"Journal of infectious diseases"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1128\/IAI.65.8.3065-3073.1997","article-title":"Mouse strain-dependent variation in the course and outcome of chlamydial genital tract infection is associated with differences in host response","volume":"65","author":"T Darville","year":"1997","journal-title":"Infection and immunity"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"4675","DOI":"10.1128\/IAI.63.12.4675-4681.1995","article-title":"Tumor necrosis factor alpha activity in genital tract secretions of guinea pigs infected with chlamydiae","volume":"63","author":"T Darville","year":"1995","journal-title":"Infection and immunity"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1128\/IAI.05022-11","article-title":"Tumor necrosis factor alpha production from CD8+ T cells mediates oviduct pathological sequelae following primary genital <italic>Chlamydia muridarum<\/italic> infection","volume":"79","author":"AK Murthy","year":"2011","journal-title":"Infection and immunity"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.4049\/jimmunol.1002952","article-title":"Immunization with live and dead <italic>Chlamydia muridarum<\/italic> induces different levels of protective immunity in a murine genital tract model: correlation with MHC class II peptide presentation and multifunctional Th1 cells","volume":"186","author":"H Yu","year":"2011","journal-title":"The journal of immunology"},{"key":"ref46","article-title":"Protection Against <italic>Chlamydia trachomatis<\/italic> Infection and Upper Genital Tract Pathological Changes by Vaccine-Promoted Neutralizing Antibodies Directed to the VD4 of the Major Outer Membrane Protein","author":"AW Olsen","year":"2015","journal-title":"The Journal of infectious diseases"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"e1003707","DOI":"10.1371\/journal.ppat.1003707","article-title":"B cells enhance antigen-specific CD4 T cell priming and prevent bacteria dissemination following <italic>Chlamydia muridarum<\/italic> genital tract infection","volume":"9","author":"LX Li","year":"2013","journal-title":"PLoS pathogens"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"8063","DOI":"10.4049\/jimmunol.0804375","article-title":"<italic>Chlamydia trachomatis<\/italic> native major outer membrane protein induces partial protection in nonhuman primates: implication for a trachoma transmission-blocking vaccine","volume":"182","author":"L Kari","year":"2009","journal-title":"The Journal of immunology"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"3456","DOI":"10.1016\/j.vaccine.2011.02.057","article-title":"Immunogenicity of a vaccine formulated with the <italic>Chlamydia trachomatis<\/italic> serovar F, native major outer membrane protein in a nonhuman primate model","volume":"29","author":"C Cheng","year":"2011","journal-title":"Vaccine"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0156094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T02:53:14Z","timestamp":1718592794000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0156094"}},"subtitle":[],"editor":[{"given":"Ashlesh K","family":"Murthy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,5,24]]},"references-count":49,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2016,5,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0156094","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,24]]}}}