{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T20:38:30Z","timestamp":1773866310207,"version":"3.50.1"},"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T00:00:00Z","timestamp":1464220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R44NS073225"],"award-info":[{"award-number":["R44NS073225"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R43MH079542"],"award-info":[{"award-number":["R43MH079542"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0156312","type":"journal-article","created":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T17:56:53Z","timestamp":1464285413000},"page":"e0156312","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities"],"prefix":"10.1371","volume":"11","author":[{"given":"Fiona Karen","family":"Harlan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason Scott","family":"Lusk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Breanna Michelle","family":"Mohr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony Peter","family":"Guzikowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert Hardy","family":"Batchelor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7038-3807","authenticated-orcid":false,"given":"John Joseph","family":"Naleway","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,5,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.4049\/jimmunol.131.1.125","article-title":"Inhibition of human natural killer activity by lysosomotropic agents","volume":"131","author":"J Verhoef","year":"1983","journal-title":"J. Immunol."},{"issue":"2","key":"ref2","first-page":"319","article-title":"Effects of lysosomotropic amines on human polymorphonuclear leucocyte function","volume":"51","author":"CM Vandenbroucke-Grauls","year":"1984","journal-title":"Immunology"},{"key":"ref3","first-page":"3","article-title":"Monodansylcadaverine (MDC) is a specific in vivo marker for autophagic vacuoles","volume":"66","author":"A Biederbick","year":"1995","journal-title":"Eur. J. Cell Biol"},{"key":"ref4","unstructured":"Zhang YZ, Diwu Z, Haugland RP. Stains for Acidic Organelles. 1995; US Patent 5869689."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"e2","DOI":"10.5334\/ojb.ab","article-title":"Cell Line and DNA Biobank From Patients Affected by Genetic Diseases","volume":"1","author":"M Filocamo","year":"2014","journal-title":"Open Journal of Bioresources"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1002\/cyto.a.22312","article-title":"Use of LysoTracker<sup>\u00ae<\/sup> dyes: a flow cytometric study of autophagy","volume":"85","author":"S Chikte","year":"2014","journal-title":"Cytometry A"},{"issue":"8","key":"ref7","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1002\/cyto.a.22495","article-title":"Evaluation of acridine orange, LysoLysoTracker<sup>\u00ae<\/sup> Red, and quinacrine as fluorescent probes for long-term tracking of acidic vesicles","volume":"85","author":"A Pierzy\u0144ska-Mach","year":"2014","journal-title":"Cytometry A"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.ejphar.2009.06.063","article-title":"Chloroquine and its analogs: A new promise of an old drug for effective and safe cancer therapies","volume":"625","author":"VR Solomon","year":"2009","journal-title":"Eur.J.Pharmacol"},{"key":"ref9","first-page":"665","article-title":"Effect of weak bases on intralysosomal pH in mouse peritoneal macrophages","volume":"63","author":"B Poole","year":"1989","journal-title":"J. Cell Biol"},{"issue":"4677","key":"ref10","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1126\/science.6505679","article-title":"Inhibitors of lysosomal enzymes: accumulation of lipofuscin-like dense bodies in the brain","volume":"226","author":"GO Ivy","year":"1984","journal-title":"Science"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.ymgme.2007.08.001","article-title":"Secondary sphingolipid accumulation in a macrophage model of Gaucher disease","volume":"92","author":"LK Hein","year":"2007","journal-title":"Mol. Genet. Metab"},{"issue":"36","key":"ref12","doi-asserted-by":"crossref","first-page":"26052","DOI":"10.1074\/jbc.M113.463562","article-title":"\u03b2-Glucosidase 2 (GBA2) activity and imino sugar pharmacology","volume":"288","author":"CM Ridley","year":"2013","journal-title":"J. Biol. Chem"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/0005-2760(86)90126-8","article-title":"beta-Glucosidase inhibition inmurine peritoneal macrophages by conduritol-B-epoxide: an in vitro model of the Gaucher cell","volume":"877","author":"DS Newburg","year":"1986","journal-title":"Biochim Biophys Acta"},{"key":"ref14","article-title":"A rapid and sensitive method for measuring N-acetylglucosaminidase activity in cultured cells","author":"V Mauri","year":"2013","journal-title":"PLOS one"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0076-6879(08)04021-4","article-title":"Monitoring autophagy in Lysosomal Storage Disorders","volume":"453","author":"N Raben","year":"2009","journal-title":"Methods Enzymol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"R100","DOI":"10.1186\/gb-2006-7-10-r100","article-title":"CellProfiler: image analysis software for identifying and quantifying cell phenotypes","volume":"7","author":"AE Carpenter","year":"2006","journal-title":"Genome Biology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/0009-8981(75)90083-2","article-title":"A microassay for Gaucher&apos;s disease","volume":"60","author":"SP Peters","year":"1975","journal-title":"Clin. Chim. Acta"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ab.2012.10.034","article-title":"A high-throughput screening assay using Krabbe Disease patient cells","volume":"434","author":"J Ribbens","year":"2013","journal-title":"Anal.Biochem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2930","DOI":"10.1038\/nprot.2007.422","article-title":"Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation","volume":"2","author":"ST Laughlin","year":"2007","journal-title":"Nature Protocols"},{"key":"ref20","first-page":"305","article-title":"Immortalization of human lymphocytes by Epstein-Barr virus","volume":"55","author":"G Miller","year":"1982","journal-title":"Yale J. Bio. Med"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0156312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T16:57:57Z","timestamp":1692377877000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0156312"}},"subtitle":[],"editor":[{"given":"Andrea","family":"Dardis","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,5,26]]},"references-count":20,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2016,5,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0156312","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,26]]}}}