{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:07:57Z","timestamp":1792674477404,"version":"4.5.0"},"reference-count":24,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T00:00:00Z","timestamp":1465948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI101391"],"award-info":[{"award-number":["AI101391"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI106738"],"award-info":[{"award-number":["AI106738"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0157234","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T19:27:02Z","timestamp":1466018822000},"page":"e0157234","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Rapid, Culture-Free Detection of Staphylococcus aureus Bacteremia"],"prefix":"10.1371","volume":"11","author":[{"given":"Elliot L.","family":"Burghardt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie S.","family":"Flenker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen C.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff","family":"Miguel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilek","family":"Ince","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Winokur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley","family":"Ford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James O.","family":"McNamara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,6,15]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/S0924-8579(02)00204-2","article-title":"Age-related trends in pathogen frequency and antimicrobial susceptibility of bloodstream isolates in North America: SENTRY Antimicrobial Surveillance Program, 1997\u20132000","volume":"20","author":"DJ Diekema","year":"2002","journal-title":"Int J Antimicrob Agents"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1086\/421946","article-title":"Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study","volume":"39","author":"H Wisplinghoff","year":"2004","journal-title":"Clin Infect Dis"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/CMR.05022-11","article-title":"Predictors of mortality in Staphylococcus aureus Bacteremia","volume":"25","author":"SJ van Hal","year":"2012","journal-title":"Clin Microbiol Rev"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jinf.2003.10.010","article-title":"Positive surveillance blood culture is a predictive factor for secondary metastatic infection in patients with Staphylococcus aureus bacteraemia","volume":"48","author":"O Lesens","year":"2004","journal-title":"J Infect"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/s150100050065","article-title":"Metastatic complications of Staphylococcus aureus septicemia. To seek is to find","volume":"28","author":"H Ringberg","year":"2000","journal-title":"Infection"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1097\/CCM.0b013e31827e83af","article-title":"Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock: 2012","volume":"41","author":"RP Dellinger","year":"2013","journal-title":"Crit Care Med"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"e18","DOI":"10.1093\/cid\/ciq146","article-title":"Clinical practice guidelines by the infectious diseases society of america for the treatment of methicillin-resistant Staphylococcus aureus infections in adults and children","volume":"52","author":"C Liu","year":"2011","journal-title":"Clin Infect Dis"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1128\/JCM.38.6.2181-2185.2000","article-title":"Frequency of low-level bacteremia in children from birth to fifteen years of age","volume":"38","author":"JA Kellogg","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00134-014-3553-8","article-title":"Accuracy of LightCycler((R)) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review and meta-analysis","volume":"41","author":"P Dark","year":"2015","journal-title":"Intensive Care Med"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"543","DOI":"10.2217\/fmb.14.8","article-title":"Nonculture techniques for the detection of bacteremia and fungemia","volume":"9","author":"F Valencia-Shelton","year":"2014","journal-title":"Future Microbiol"},{"issue":"5","key":"ref11","first-page":"191","article-title":"Desoxyribonuclease and coagulase activity of micrococci","volume":"9","author":"JW Disalvo","year":"1958","journal-title":"Med Techn Bull"},{"issue":"1","key":"ref12","first-page":"172","article-title":"The structural basis of the catalytic function of staphylococcal nuclease","volume":"21","author":"P Cuatrecasas","year":"1968","journal-title":"Brookhaven Symp Biol"},{"issue":"7","key":"ref13","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1016\/S0021-9258(18)96126-0","article-title":"Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus","volume":"242","author":"P Cuatrecasas","year":"1967","journal-title":"J Biol Chem"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1128\/AEM.23.5.994-997.1972","article-title":"Tolerance of staphylococcal thermonuclease to stress","volume":"23","author":"RV Lachica","year":"1972","journal-title":"Appl Microbiol"},{"issue":"10","key":"ref15","doi-asserted-by":"crossref","first-page":"3478","DOI":"10.1128\/JCM.01477-07","article-title":"Thermostable DNase is superior to tube coagulase for direct detection of Staphylococcus aureus in positive blood cultures","volume":"45","author":"PR Lagace-Wiens","year":"2007","journal-title":"J Clin Microbiol"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1128\/JCM.18.3.722-724.1983","article-title":"Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing","volume":"18","author":"BM Madison","year":"1983","journal-title":"J Clin Microbiol"},{"issue":"7","key":"ref17","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1136\/jcp.30.7.606","article-title":"Comparison of coagulase, deoxyribonuclease (DNase), and heat-stable nuclease tests for identification of Staphylococcus aureus","volume":"30","author":"RE Menzies","year":"1977","journal-title":"J Clin Pathol"},{"issue":"18","key":"ref18","doi-asserted-by":"crossref","first-page":"3696","DOI":"10.1093\/nar\/27.18.3696","article-title":"Hypersensitive substrate for ribonucleases","volume":"27","author":"BR Kelemen","year":"1999","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/nm.3460","article-title":"Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe","volume":"20","author":"FJ Hernandez","year":"2014","journal-title":"Nat Med"},{"issue":"11","key":"ref20","doi-asserted-by":"crossref","first-page":"e26714","DOI":"10.1371\/journal.pone.0026714","article-title":"Nuclease modulates biofilm formation in community-associated methicillin-resistant Staphylococcus aureus","volume":"6","author":"MR Kiedrowski","year":"2011","journal-title":"PloS one"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1093\/infdis\/jiu091","article-title":"Increased neutrophil extracellular trap-mediated Staphylococcus aureus clearance through inhibition of nuclease activity by clindamycin and immunoglobulin","volume":"210","author":"K Schilcher","year":"2014","journal-title":"J Infect Dis"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"e2041","DOI":"10.1371\/journal.pone.0002041","article-title":"Attomolar detection of botulinum toxin type A in complex biological matrices","volume":"3","author":"K Bagramyan","year":"2008","journal-title":"PloS one"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/j.1699-0463.1995.tb01098.x","article-title":"Comparison of tests designed to identify Staphylococcus aureus thermostable nuclease","volume":"103","author":"OG Brakstad","year":"1995","journal-title":"APMIS"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1086\/502580","article-title":"Clinical outcomes and costs due to Staphylococcus aureus bacteremia among patients receiving long-term hemodialysis","volume":"26","author":"JJ Engemann","year":"2005","journal-title":"Infect Control Hosp Epidemiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0157234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T19:47:02Z","timestamp":1656704822000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0157234"}},"subtitle":[],"editor":[{"given":"Holger","family":"Rohde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,6,15]]},"references-count":24,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2016,6,15]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0157234","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,15]]}}}