{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T04:23:41Z","timestamp":1768796621217,"version":"3.49.0"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T00:00:00Z","timestamp":1466380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Major Projects of Natural Science Research in Universities and Colleges in Jiangsu Province","award":["15KJA240001"],"award-info":[{"award-number":["15KJA240001"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31302221"],"award-info":[{"award-number":["31302221"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the National Natural Science Foundation of Jiangsu Province","award":["31302221 and 31272666"],"award-info":[{"award-number":["31302221 and 31272666"]}]},{"name":"the \u201cQinglan\u201d project of Jiangsu province"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0157544","type":"journal-article","created":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T17:36:45Z","timestamp":1466444205000},"page":"e0157544","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Cloning and Expression of \u03b2-Defensin from Soiny Mullet (Liza haematocheila), with Insights of its Antibacterial Mechanism"],"prefix":"10.1371","volume":"11","author":[{"given":"Zhitao","family":"Qi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fancui","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qihuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenglung","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,6,20]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nri1180","article-title":"Defensins: antimicrobial peptides of innate immunity","volume":"3","author":"T Ganz","year":"2003","journal-title":"Nat Rev Immunol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.dci.2012.02.011","article-title":"Evolutionary origin of beta-defensins","volume":"39","author":"S Zhu","year":"2013","journal-title":"Dev Comp Immunol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1007\/s00018-011-0715-5","article-title":"Retrocyclins and their activity against HIV-1","volume":"68","author":"WT Penberthy","year":"2011","journal-title":"Cell Mol Life Sci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1021\/bi002519d","article-title":"The NMR structure of human \u03b2-defensin-2 reveals a novel alpha-helical segment","volume":"40","author":"MV Sawai","year":"2001","journal-title":"Biochemistry"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.molimm.2006.01.012","article-title":"Discovery of multiple \u03b2-defensin like homologues in teleost","volume":"44","author":"J Zou","year":"2007","journal-title":"Mol Immunol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.cbpb.2013.07.006","article-title":"Molecular cloning and expression analysis of two \u03b2-defensin genes in the blunt snout bream (<italic>Megalobrama amblycephala<\/italic>)","volume":"166","author":"T Liang","year":"2013","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.gene.2015.04.025","article-title":"\u03b2-defensin in Nile tilapia (<italic>Orechromis niloticus<\/italic>): sequence, tissue expression, and anti-bacterial activity of synthetic peptides","volume":"566","author":"JJ Dong","year":"2015","journal-title":"Gene"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.fsi.2009.11.004","article-title":"Multiple beta-defensin isoforms identified in early developmental stages of the teleost <italic>Paralichthys olivaceus<\/italic>","volume":"28","author":"BH Nam","year":"2010","journal-title":"Fish Shellfish Immunol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.fsi.2012.06.003","article-title":"Expression pattern, promoter activity and bactericidal property of beta-defensin from the mandarin fish <italic>Siniperca chuatsi<\/italic>","volume":"33","author":"G Wang","year":"2012","journal-title":"Fish Shellfish Immunol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.fsi.2012.02.005","article-title":"Antiviral effects of beta-defensin derived from orange-spotted grouper (<italic>Epinephelus coioides<\/italic>)","volume":"32","author":"M Guo","year":"2012","journal-title":"Fish Shellfish Immunol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/j.molimm.2011.03.022","article-title":"Molecular and functional characterization of the gilthead seabream beta-defensin demonstrate its chemotactic and antimicrobial activity","volume":"48","author":"A Cuesta","year":"2011","journal-title":"Mol Immunol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.antiviral.2013.11.009","article-title":"In addition to its antiviral and immunomodulatory properties, the zebrafish beta-defensin 2 (zfBD2) is a potent viral DNA vaccine molecular adjuvant","volume":"101","author":"P Garcia-Valtanen","year":"2014","journal-title":"Antiviral Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"607","DOI":"10.3390\/biology4040607","article-title":"Antimicrobial peptides as mediators of innate immunity in teleost","volume":"4","author":"BA Katzenback","year":"2015","journal-title":"Biology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1021\/cb1001558","article-title":"Describing the mechanism of antimicrobial peptide action with the interfacial activity model","volume":"5","author":"WC Wimley","year":"2010","journal-title":"ACS Chem Biol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2478\/v10136-009-0019-3","article-title":"Membrane-active peptides as anti-infectious agents","volume":"8","author":"L Rivas","year":"2010","journal-title":"J Appl Biomed"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.bbamem.2012.12.015","article-title":"Molecular simulations suggest how a branched antimicrobial peptide perturbs a bacterial membrane and enhances permeability","volume":"1828","author":"JG Li","year":"2013","journal-title":"Biochim Biophys Acta"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.fsi.2015.12.029","article-title":"Transcriptome analysis of soiny mullet (<italic>Liza haematocheila<\/italic>) spleen in response to <italic>Streptococcus dysgalactiae<\/italic>","volume":"49","author":"ZT Qi","year":"2016","journal-title":"Fish Shellfish Immunol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1093\/bib\/bbn017","article-title":"MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences","volume":"9","author":"S Kumar","year":"2008","journal-title":"Brief Bioinform"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.molimm.2014.07.006","article-title":"Molecular cloning, expression analysis and functional characterization of interleukin-22 in soiny mullet, <italic>Liza haematocheila<\/italic>","volume":"63","author":"ZT Qi","year":"2015","journal-title":"Mol Immunol"},{"key":"ref20","unstructured":"Prime, version 2.1, Schr\u00f6dinger, LLC, New York, NY, 2009."},{"key":"ref21","doi-asserted-by":"crossref","first-page":"39021","DOI":"10.1074\/jbc.M103830200","article-title":"The structure of human \u03b2-defensin-1: new insights into structural properties of \u03b2-defensins","volume":"276","author":"DM Hoover","year":"2001","journal-title":"J Biol Chem"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"W363","DOI":"10.1093\/nar\/gki481","article-title":"PatchDock and SymmDock: servers for rigid and symmetric docking","volume":"33","author":"D Schneidman-Duhovny","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1002\/prot.20562","article-title":"Geometry based flexible and symmetric protein docking","volume":"60","author":"D Schneidman-Duhovny","year":"2005","journal-title":"Proteins"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1021\/ct700301q","article-title":"GROMACS 4: Algorithms for highly efficient, load-balanced, and scalable molecular simulation","volume":"4","author":"B Hess","year":"2008","journal-title":"J Chem Theory Comput"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1002\/prot.21123","article-title":"Comparison of multiple Amber force fields and development of improved protein backbone parameters","volume":"65","author":"V Hornak","year":"2006","journal-title":"Proteins"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s00232-010-9296-8","article-title":"Lipidbook: A public repository for force field parameters used in membrane simulations","volume":"236","author":"J Doma\u0144ski","year":"2010","journal-title":"J Membrane Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1016\/j.biochi.2008.02.025","article-title":"Role of phosphatidylglycerols in the stability of bacterial membranes","volume":"90","author":"W Zhao","year":"2008","journal-title":"Biochimie"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1021\/jp212503e","article-title":"Derivation and systematic validation of a refined all-atom force field for phosphatidylcholine lipids","volume":"116","author":"JP J\u00e4mbeck","year":"2012","journal-title":"J Phys Chem B"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1021\/ct300342n","article-title":"An extension and further validation of an all-atomistic force field for biological membranes","volume":"8","author":"JP J\u00e4mbeck","year":"2012","journal-title":"J Chem Theory Comput"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s12602-015-9197-3","article-title":"Molecular characterization and in silico analysis of defensin from Tor putitora (Hamilton)","volume":"7","author":"P Chaturvedi","year":"2015","journal-title":"Probiotics Antimicro Prot"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/psc.749","article-title":"Synthesis and structure-activity relationship of \u03b2-defensins, multi-functional peptides of the immune system","volume":"12","author":"E Kluver","year":"2006","journal-title":"J Pept Sci"},{"key":"ref32","first-page":"105","article-title":"Molecular evolutionary analysis \u03b2-defensin peptides in vertebrates","volume":"11","author":"J Tu","year":"2015","journal-title":"Evol Bioinform Online"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"3358","DOI":"10.1016\/j.molimm.2009.07.018","article-title":"Characterization of three novel \u03b2-defensin antimicrobial peptides in rainbow trout (<italic>Oncorhynchus mykiss<\/italic>)","volume":"46","author":"E Casadei","year":"2009","journal-title":"Mol Immunol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"32911","DOI":"10.1074\/jbc.M006098200","article-title":"The structure of human \u03b2-defensin shows evidence of higher order oligomerization","volume":"275","author":"DM Hoover","year":"2000","journal-title":"J Biol Chem"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1110\/ps.04702104","article-title":"Cation-\u03c0 interactions studied in a model coiled-coil peptide","volume":"13","author":"MM Slutsky","year":"2004","journal-title":"Protein Sci"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/jcc.22885","article-title":"Multiwfn: a multifunctional wave function analyzer","volume":"35","author":"T Lu","year":"2012","journal-title":"J Comp Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1529\/biophysj.106.086272","article-title":"Atomic-scale structure and electrostatics of anionic palmitoyloleoylphosphatidylglycerol lipid bilayers with Na+ counterions","volume":"92","author":"W Zhao","year":"2007","journal-title":"Biophys J"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1016\/j.bbamem.2014.07.020","article-title":"Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection florescence microscopy","volume":"1838","author":"AT Ngo","year":"2014","journal-title":"Biochim Biohpys Acta"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1002\/pro.5560020813","article-title":"Defensins promote fusion and lysis of negatively charged membranes","volume":"2","author":"G Fujii","year":"1993","journal-title":"Protein Sci"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ejmech.2014.08.021","article-title":"Membrane disruptive antimicrobial activities of human\u2013defensin-3 analogs","volume":"91","author":"US Sudheendra","year":"2015","journal-title":"Eur J Med Chem"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0157544","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T12:24:47Z","timestamp":1589027087000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0157544"}},"subtitle":[],"editor":[{"given":"Zhan","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,6,20]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2016,6,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0157544","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,20]]}}}