{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:18:43Z","timestamp":1792034323544,"version":"4.4.0"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T00:00:00Z","timestamp":1468281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Danish Council for Independent Research (FTP)"},{"DOI":"10.13039\/100008398","name":"Villum Fonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0158968","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T20:16:56Z","timestamp":1468354616000},"page":"e0158968","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Antigen Uptake during Different Life Stages of Zebrafish (Danio rerio) Using a GFP-Tagged Yersinia ruckeri"],"prefix":"10.1371","volume":"11","author":[{"given":"Rozalia","family":"Korbut","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Foojan","family":"Mehrdana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per Walter","family":"Kania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne Halberg","family":"Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorte","family":"Frees","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inger","family":"Dalsgaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise von Gersdorff","family":"J\u00f8rgensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,7,12]]},"reference":[{"key":"ref1","first-page":"x","author":"AE Ellis","year":"1988"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.vetimm.2013.04.001","article-title":"Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections","volume":"154","author":"JK Chettri","year":"2013","journal-title":"Veterinary Immunology and Immunopathology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1365-2761.1982.tb00475.x","article-title":"Duration of Immunity in Salmonids Vaccinated by Direct Immersion with Yersinia ruckeri and Vibrio anguillarum Bacterins","volume":"5","author":"KA Johnson","year":"1982","journal-title":"Journal of Fish Diseases"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.micpath.2013.03.001","article-title":"Tissue specific uptake of inactivated and live Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss): Visualization by immunohistochemistry and in situ hybridization","volume":"59\u201360","author":"U Khimmakthong","year":"2013","journal-title":"Microbial Pathogenesis"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0089672","article-title":"3D Visualization of the Initial Yersinia ruckeri Infection Route in Rainbow Trout (Oncorhynchus mykiss) by Optical Projection Tomography","volume":"9","author":"M Ohtani","year":"2014","journal-title":"Plos One"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/sj.hdy.6800635","article-title":"Genome evolution and biodiversity in teleost fish","volume":"94","author":"JN Volff","year":"2005","journal-title":"Heredity"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/raq.12041","article-title":"The zebrafish (Danio rerio) as a model organism, with emphasis on applications for finfish aquaculture research","volume":"6","author":"L Ribas","year":"2014","journal-title":"Reviews in Aquaculture"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nrg2091","article-title":"Animal models of human disease: zebrafish swim into view","volume":"8","author":"GJ Lieschke","year":"2007","journal-title":"Nature Reviews Genetics"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1126\/science.1066652","article-title":"Genomics\u2014Zebrafish\u2014The canonical vertebrate","volume":"294","author":"MC Fishman","year":"2001","journal-title":"Science"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nrd1606","article-title":"In vivo drug discovery in the zebrafish","volume":"4","author":"LI Zon","year":"2005","journal-title":"Nature Reviews Drug Discovery"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"227","DOI":"10.2144\/05392RV02","article-title":"Making waves in cancer research: new models in the zebrafish","volume":"39","author":"S Berghmans","year":"2005","journal-title":"Biotechniques"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1002\/bdrc.20210","article-title":"Developmental Toxicity Screening in Zebrafish","volume":"93","author":"CW McCollum","year":"2011","journal-title":"Birth Defects Research Part C-Embryo Today-Reviews"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1052>3.3.CO;2-B","article-title":"Zebrafish neural induction and patterning","volume":"219","author":"B Appel","year":"2000","journal-title":"Developmental Dynamics"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/S1286-4579(02)00029-1","article-title":"Zebrafish as an immunological model system","volume":"4","author":"JA Yoder","year":"2002","journal-title":"Microbes and Infection"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.dci.2007.11.011","article-title":"Immunology and zebrafish: Spawning new models of human disease","volume":"32","author":"ND Meeker","year":"2008","journal-title":"Developmental and Comparative Immunology"},{"key":"ref16","first-page":"253","article-title":"The zebrafish as a model organism to study development of the immune system","volume":"81","author":"D Traver","year":"2003","journal-title":"Advances in Immunology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.fsi.2008.05.005","article-title":"Zebrafish as a model for infectious disease and immune function","volume":"25","author":"C Sullivan","year":"2008","journal-title":"Fish & Shellfish Immunology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/978-1-4614-0106-3_15","article-title":"Zebrafish: Model for the Study of Inflammation and the Innate Immune Response to Infectious Diseases","volume":"946","author":"B Novoa","year":"2012","journal-title":"Current Topics in Innate Immunity Ii"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.fsi.2008.05.002","article-title":"Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila","volume":"25","author":"I Rodriguez","year":"2008","journal-title":"Fish & Shellfish Immunology"},{"key":"ref20","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2180-12-37","article-title":"Impact of antibiotic treatments on the expression of the R plasmid tra genes and on the host innate immune activity during pRAS1 bearing Aeromonas hydrophila infection in zebrafish (Danio rerio)","volume":"12","author":"L Cantas","year":"2012","journal-title":"Bmc Microbiology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.molimm.2006.03.001","article-title":"Acute phase response in zebrafish upon Aeromonas salmonicida and Staphylococcus aureus infection: Striking similarities and obvious differences with mammals","volume":"44","author":"B Lin","year":"2007","journal-title":"Molecular Immunology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"5806","DOI":"10.1016\/j.vaccine.2006.05.015","article-title":"Zebrafish (Danio rerio) as a model for the study of vaccination against viral haemorrhagic septicemia virus (VHSV)","volume":"24","author":"B Novoa","year":"2006","journal-title":"Vaccine"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fsi.2012.03.031","article-title":"Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate","volume":"33","author":"ZH Zhang","year":"2012","journal-title":"Fish & Shellfish Immunology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3389\/fgene.2014.00313","article-title":"Zebrafish as animal model for aquaculture nutrition research","volume":"5","author":"PE Ulloa","year":"2014","journal-title":"Front Genet"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/s11160-011-9203-0","article-title":"Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes","volume":"21","author":"PE Ulloa","year":"2011","journal-title":"Reviews in Fish Biology and Fisheries"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.cbd.2011.02.002","article-title":"Whole body proteome response to a dietary lysine imbalance in zebrafish Danio rerio","volume":"6","author":"P Gomez-Requeni","year":"2011","journal-title":"Comparative Biochemistry and Physiology D-Genomics & Proteomics"},{"key":"ref27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002815","article-title":"A New Zebrafish Model of Oro-Intestinal Pathogen Colonization Reveals a Key Role for Adhesion in Protection by Probiotic Bacteria","volume":"8","author":"O Rendueles","year":"2012","journal-title":"Plos Pathogens"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"3757","DOI":"10.1242\/dev.126.17.3757","article-title":"nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate","volume":"126","author":"JA Lister","year":"1999","journal-title":"Development"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1093\/genetics\/103.1.125","article-title":"Induction of Mutations by Gamma-Rays in Pre-Gonial Germ-Cells of Zebrafish Embryos","volume":"103","author":"C Walker","year":"1983","journal-title":"Genetics"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0923-2508(00)00158-3","article-title":"Optimization of green fluorescent protein expression vectors for in vitro and in vivo detection of Listeria monocytogenes","volume":"151","author":"N Fortineau","year":"2000","journal-title":"Research in Microbiology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1242\/bio.20135132","article-title":"transparent, a gene affecting stripe formation in Zebrafish, encodes the mitochondrial protein Mpv17 that is required for iridophore survival","volume":"2","author":"J Krauss","year":"2013","journal-title":"Biology Open"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.stem.2007.11.002","article-title":"Transparent adult zebrafish as a tool for in vivo transplantation analysis","volume":"2","author":"RM White","year":"2008","journal-title":"Cell Stem Cell"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1111\/j.1365-2761.2011.01291.x","article-title":"Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)","volume":"34","author":"LV Jorgensen","year":"2011","journal-title":"Journal of Fish Diseases"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1006\/dbio.2001.0393","article-title":"Zebrafish early macrophages colonize cephalic mesenchyme and developing brain, retina, and epidermis through, a M-CSF receptor-dependent invasive process","volume":"238","author":"P Herbomel","year":"2001","journal-title":"Developmental Biology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.fsi.2004.09.005","article-title":"Ontogeny of the immune system of fish","volume":"20","author":"A Zapata","year":"2006","journal-title":"Fish & Shellfish Immunology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.ydbio.2005.07.013","article-title":"Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis","volume":"286","author":"ANY Ng","year":"2005","journal-title":"Developmental Biology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.mod.2004.10.009","article-title":"Intestinal growth and differentiation in zebrafish","volume":"122","author":"KN Wallace","year":"2005","journal-title":"Mechanisms of Development"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1128\/IAI.00642-12","article-title":"Innate Immune Response to Streptococcus iniae Infection in Zebrafish Larvae","volume":"81","author":"EA Harvie","year":"2013","journal-title":"Infection and Immunity"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"486","DOI":"10.17221\/3294-VETMED","article-title":"Innate and adaptive immunity in teleost fish: a review","volume":"56","author":"C Uribe","year":"2011","journal-title":"Veterinarni Medicina"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.dci.2011.08.014","article-title":") Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckerie","volume":"36","author":"JK Chettri","year":"2012","journal-title":"Developmental and Comparative Immunology"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0145-305X(03)00103-4","article-title":"Development and maturation of the immune system in zebrafish, Danio rerio: a gene expression profiling, in situ hybridization and immunological study","volume":"28","author":"SH Lam","year":"2004","journal-title":"Developmental and Comparative Immunology"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"7233","DOI":"10.1038\/sj.onc.1207943","article-title":"The &apos;definitive&apos; (and &apos;primitive&apos;) guide to zebrafish hematopoiesis","volume":"23","author":"AJ Davidson","year":"2004","journal-title":"Oncogene"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"602","DOI":"10.2307\/1447790","article-title":"Quantifying developmental progress for comparative studies of larval fishes","author":"LA Fuiman","year":"1998","journal-title":"Copeia"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1242\/dev.129.3.597","article-title":"Postembryonic development of the posterior lateral line in zebrafish","volume":"129","author":"V Ledent","year":"2002","journal-title":"Development"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.vaccine.2014.12.055","article-title":"Evaluation of dual nasal delivery of infectious hematopoietic necrosis virus and enteric red mouth vaccines in rainbow trout (Oncorhynchus mykiss)","volume":"33","author":"S LaPatra","year":"2015","journal-title":"Vaccine"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"3655","DOI":"10.1128\/JVI.80.7.3655-3659.2006","article-title":"Bioluminescence imaging of live infected salmonids reveals that the fin bases are the major portal of entry for Novirhabdovirus","volume":"80","author":"A Harmache","year":"2006","journal-title":"J Virol"},{"key":"ref47","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2164-11-518","article-title":"Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach","volume":"11","author":"P Encinas","year":"2010","journal-title":"Bmc Genomics"},{"key":"ref48","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001269","article-title":"Whole-Body Analysis of a Viral Infection: Vascular Endothelium is a Primary Target of Infectious Hematopoietic Necrosis Virus in Zebrafish Larvae","volume":"7","author":"M Ludwig","year":"2011","journal-title":"Plos Pathogens"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.fsi.2013.11.007","article-title":"iTRAQ analysis of gill proteins from the zebrafish (Danio rerio) infected with Aeromonas hydrophila","volume":"36","author":"AJ Lu","year":"2014","journal-title":"Fish & Shellfish Immunology"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"3955","DOI":"10.1016\/j.vaccine.2014.05.010","article-title":"Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS\/dsRNA-loaded nanoliposomes","volume":"32","author":"A Ruyra","year":"2014","journal-title":"Vaccine"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aquatox.2012.04.012","article-title":"Uptake of silver nanoparticles and toxicity to early life stages of Japanese medaka (Oryzias latipes): Effect of coating materials","volume":"120","author":"KWH Kwok","year":"2012","journal-title":"Aquatic Toxicology"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1111\/j.1095-8649.2011.03080.x","article-title":"Effects of manufactured nanomaterials on fishes: a target organ and body systems physiology approach","volume":"79","author":"RD Handy","year":"2011","journal-title":"Journal of Fish Biology"},{"key":"ref53","first-page":"363","article-title":"Invitro Antigen Trapping by Gill Cells of the Rainbow-Trout\u2014an Immunohistochemical Study","volume":"8","author":"M Torroba","year":"1993","journal-title":"Histology and Histopathology"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S1050-4648(05)80023-3","article-title":"Evidence for Oral Ingestion as the Principal Route of Antigen Entry in Bath-Immunized Fish","volume":"5","author":"RA Robohm","year":"1995","journal-title":"Fish & Shellfish Immunology"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1006\/fsim.1998.0181","article-title":"The morphology of the immune system in teleost fishes","volume":"9","author":"CM Press","year":"1999","journal-title":"Fish & Shellfish Immunology"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/0021-9975(76)90005-0","article-title":"The relationship between ellipsoids and melano-macrophage centres in the spleen of turbot (Scophthalmus maximus)","volume":"86","author":"HW Ferguson","year":"1976","journal-title":"J Comp Pathol"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1095-8649.1984.tb04854.x","article-title":"The Tissue Localization of Aeromonas-Salmonicida in Rainbow-Trout, Salmo-Gairdneri Richardson, Following 3 Methods of Administration","volume":"25","author":"MF Tatner","year":"1984","journal-title":"Journal of Fish Biology"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1095-8649.1987.tb05315.x","article-title":"Electron-Microscopic Examination of Antigen Uptake by Salmonid Gill Cells after Bath Immunization with a Bacterin","volume":"31","author":"AG Zapata","year":"1987","journal-title":"Journal of Fish Biology"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0158968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,3]],"date-time":"2022-07-03T00:48:15Z","timestamp":1656809295000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0158968"}},"subtitle":[],"editor":[{"given":"Pierre","family":"Boudinot","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,7,12]]},"references-count":58,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2016,7,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0158968","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,12]]}}}