{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:09:11Z","timestamp":1780718951265,"version":"3.54.1"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T00:00:00Z","timestamp":1468886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Faculty Mannheim, Heidelberg University"},{"name":"Medical Faculty Mannheim, Heidelberg University"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0159331","type":"journal-article","created":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T17:47:42Z","timestamp":1468950462000},"page":"e0159331","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Porcine Animal Model for Early Meniscal Degeneration \u2013 Analysis of Histology, Gene Expression and Magnetic Resonance Imaging Six Months after Resection of the Anterior Cruciate Ligament"],"prefix":"10.1371","volume":"11","author":[{"given":"Michael","family":"Kreinest","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor","family":"Reisig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Str\u00f6bel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Dinter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Attenberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Lipp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Schwarz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,7,19]]},"reference":[{"issue":"252","key":"ref1","first-page":"19","article-title":"Material properties and structure-function relationships in the menisci","author":"DC Fithian","year":"1990","journal-title":"Clinical orthopaedics and related research"},{"key":"ref2","first-page":"323","article-title":"Meniscus structure and function","volume":"54","author":"EM Wojtys","year":"2005","journal-title":"Instructional course lectures"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.bbrc.2008.12.111","article-title":"Gene expression profiles of dynamically compressed single chondrocytes and chondrons","volume":"379","author":"QG Wang","year":"2009","journal-title":"Biochemical and biophysical research communications"},{"issue":"11","key":"ref4","doi-asserted-by":"crossref","first-page":"3511","DOI":"10.1002\/art.22973","article-title":"Tribology: a portal to understand joint failure?","volume":"56","author":"MA Wimmer","year":"2007","journal-title":"Arthritis and rheumatism"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"40","DOI":"10.22203\/eCM.v013a04","article-title":"Bioregulation of lubricin expression by growth factors and cytokines","volume":"13","author":"AR Jones","year":"2007","journal-title":"Eur Cell Mater"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.orthres.2004.11.011","article-title":"Proteoglycan 4 (PRG4) synthesis and immunolocalization in bovine meniscus","volume":"23","author":"BL Schumacher","year":"2005","journal-title":"J Orthop Res"},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1002\/art.22174","article-title":"Large-scale gene expression profiling reveals major pathogenetic pathways of cartilage degeneration in osteoarthritis","volume":"54","author":"T Aigner","year":"2006","journal-title":"Arthritis and rheumatism"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"49","DOI":"10.22203\/eCM.v012a06","article-title":"IL-1beta and BMPs\u2014interactive players of cartilage matrix degradation and regeneration","volume":"12","author":"T Aigner","year":"2006","journal-title":"European cells & materials"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1749-799X-1-8","article-title":"Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs","volume":"1","author":"AM Stoker","year":"2006","journal-title":"Journal of orthopaedic surgery and research"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"R156","DOI":"10.1186\/ar1471","article-title":"Early and stable upregulation of collagen type II, collagen type I and YKL40 expression levels in cartilage during early experimental osteoarthritis occurs independent of joint location and histological grading","volume":"7","author":"H Lorenz","year":"2005","journal-title":"Arthritis research & therapy"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.orthres.2004.06.016","article-title":"Spatial organization of types I and II collagen in the canine meniscus","volume":"23","author":"HE Kambic","year":"2005","journal-title":"J Orthop Res"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1002\/art.20725","article-title":"Freshly isolated osteoarthritic chondrocytes are catabolically more active than normal chondrocytes, but less responsive to catabolic stimulation with interleukin-1beta","volume":"52","author":"Z Fan","year":"2005","journal-title":"Arthritis Rheum"},{"issue":"3\u20134","key":"ref13","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s00418-005-0005-0","article-title":"Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study","volume":"124","author":"J Melrose","year":"2005","journal-title":"Histochemistry and cell biology"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1053\/joca.2000.0347","article-title":"Age-related changes in the synthesis and mRNA expression of decorin and aggrecan in human meniscus and articular cartilage","volume":"9","author":"A McAlinden","year":"2001","journal-title":"Osteoarthritis Cartilage"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.arthro.2004.03.010","article-title":"Expression changes of gelatinases in human osteoarthritic knees and arthroscopic debridement","volume":"20","author":"YS Hsieh","year":"2004","journal-title":"Arthroscopy: the journal of arthroscopic & related surgery: official publication of the Arthroscopy Association of North America and the International Arthroscopy Association"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s10038-003-0004-8","article-title":"Expression profiles of two types of human knee-joint cartilage","volume":"48","author":"K Ochi","year":"2003","journal-title":"J Hum Genet"},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1007\/s00428-006-0221-6","article-title":"C4d deposits mark sites of meniscal tissue disintegration","volume":"449","author":"A Dankof","year":"2006","journal-title":"Virchows Archiv: an international journal of pathology"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1136\/ard.32.4.387","article-title":"Experimentally-induced osteoarthritis in the dog","volume":"32","author":"MJ Pond","year":"1973","journal-title":"Annals of the rheumatic diseases"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1007\/s00330-004-2486-y","article-title":"Early changes in experimental osteoarthritis using the Pond-Nuki dog model: technical procedure and initial results of in vivo MR imaging","volume":"15","author":"M Libicher","year":"2005","journal-title":"European radiology"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1177\/03635465010290021801","article-title":"The biomechanical interdependence between the anterior cruciate ligament replacement graft and the medial meniscus","volume":"29","author":"CD Papageorgiou","year":"2001","journal-title":"The American journal of sports medicine"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1148\/radiology.217.1.r00se36193","article-title":"Characterization of the "red zone" of knee meniscus: MR imaging and histologic correlation","volume":"217","author":"O Hauger","year":"2000","journal-title":"Radiology"},{"issue":"5","key":"ref22","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1007\/s001670050163","article-title":"Magnetic resonance imaging of meniscal degeneration in torn menisci: a comparison between anterior cruciate ligament deficient knees and stable knees","volume":"7","author":"K Nawata","year":"1999","journal-title":"Knee surgery, sports traumatology, arthroscopy: official journal of the ESSKA"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1258\/la.2007.06029e","article-title":"The minipig model for experimental chondral and osteochondral defect repair in tissue engineering: retrospective analysis of 180 defects","volume":"42","author":"T Gotterbarm","year":"2008","journal-title":"Laboratory animals"},{"issue":"Suppl 2","key":"ref24","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1600-0773.1997.tb01980.x","article-title":"The Gottingen minipig in pharmacology and toxicology","volume":"80","author":"P Bollen","year":"1997","journal-title":"Pharmacology & toxicology"},{"issue":"Pt 3","key":"ref25","first-page":"371","article-title":"Collagen orientations in the meniscus of the knee joint","volume":"140","author":"RM Aspden","year":"1985","journal-title":"Journal of anatomy"},{"issue":"Pt 1","key":"ref26","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1046\/j.1469-7580.1998.19310023.x","article-title":"Collagen fibre arrangement in the tibial plateau articular cartilage of man and other mammalian species","volume":"193","author":"MJ Kaab","year":"1998","journal-title":"Journal of anatomy"},{"key":"ref27","first-page":"521","article-title":"BREEDING OF A MINIATURE SWINE AS AN EXPERIMENTAL AND LABORATORY ANIMAL","volume":"189","author":"F Haring","year":"1963","journal-title":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene 1 Abt Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie Originale"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1007\/s11999-014-3974-2","article-title":"Validation of porcine knee as a sex-specific model to study human anterior cruciate ligament disorders","volume":"473","author":"AM Kiapour","year":"2015","journal-title":"Clinical orthopaedics and related research"},{"key":"ref29","first-page":"13","article-title":"Scintigraphic evaluation of bone formation in G\u00f6ttingen minipigs","volume":"37","author":"ML Schwarz","year":"2010","journal-title":"Scand J lab Anim Sci"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/S0749-8063(05)80061-1","article-title":"Magnetic resonance imaging (MRI) and arthroscopy in the detection of meniscal degenerations: correlation of arthroscopy and MRI with histology findings","volume":"10","author":"J Raunest","year":"1994","journal-title":"Arthroscopy: the journal of arthroscopic & related surgery: official publication of the Arthroscopy Association of North America and the International Arthroscopy Association"},{"issue":"12","key":"ref31","doi-asserted-by":"crossref","first-page":"9492","DOI":"10.1074\/jbc.M008528200","article-title":"Hydrogen peroxide-induced structural alterations of RNAse A","volume":"276","author":"P Lasch","year":"2001","journal-title":"The Journal of biological chemistry"},{"key":"ref32","article-title":"Differences in menisci from stifle joints of juvenile and adult pigs\u2013analysis of gene expression and TEM evaluation of collagen I fibers","author":"M Kreinest","year":"2015","journal-title":"accepted by Comp med"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/1471-2199-8-67","article-title":"Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR","volume":"8","author":"AB Nygard","year":"2007","journal-title":"BMC molecular biology"},{"issue":"9","key":"ref34","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1007\/s11999-008-0346-9","article-title":"Statistics in brief: the importance of sample size in the planning and interpretation of medical research","volume":"466","author":"DJ Biau","year":"2008","journal-title":"Clinical orthopaedics and related research"},{"key":"ref35","article-title":"Leitlinien f\u00fcr Diagnostik und Therapie in der Neurologie","author":"A Curt","year":"2012"},{"issue":"6","key":"ref36","doi-asserted-by":"crossref","first-page":"e1000412","DOI":"10.1371\/journal.pbio.1000412","article-title":"Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research","volume":"8","author":"C Kilkenny","year":"2010","journal-title":"PLoS biology"},{"issue":"146","key":"ref37","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1097\/00003086-198001000-00043","article-title":"Lesions of the meniscus. Part I: Macroscopic and histologic findings","author":"O Ferrer-Roca","year":"1980","journal-title":"Clinical orthopaedics and related research"},{"issue":"5","key":"ref38","first-page":"475","article-title":"Meniscal degeneration score and NITEGE expression: immunohistochemical detection of NITEGE in advanced meniscal degeneration","volume":"39","author":"V Krenn","year":"2010","journal-title":"Orthop\u00e4de"},{"issue":"7","key":"ref39","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1007\/s00393-010-0609-1","article-title":"Histopathological degeneration score of fibrous cartilage. Low- and high-grade meniscal degeneration","volume":"69","author":"V Krenn","year":"2010","journal-title":"Zeitschrift fur Rheumatologie"},{"issue":"10","key":"ref40","doi-asserted-by":"crossref","first-page":"2632","DOI":"10.1002\/art.10530","article-title":"The influence of alignment on risk of knee osteoarthritis progression according to baseline stage of disease","volume":"46","author":"R Cerejo","year":"2002","journal-title":"Arthritis and rheumatism"},{"issue":"4","key":"ref41","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1002\/art.24407","article-title":"Notochordal intervertebral disc cells: sensitivity to nutrient deprivation","volume":"60","author":"T Guehring","year":"2009","journal-title":"Arthritis and rheumatism"},{"issue":"5","key":"ref42","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s00402-007-0485-6","article-title":"Diagnostics of degenerative meniscal tears at 3-Tesla MRI compared to arthroscopy as reference standard","volume":"128","author":"LV von Engelhardt","year":"2008","journal-title":"Archives of orthopaedic and trauma surgery"},{"issue":"11","key":"ref43","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1016\/j.joca.2010.07.012","article-title":"Cartilage and meniscus assessment using T1rho and T2 measurements in healthy subjects and patients with osteoarthritis","volume":"18","author":"ZA Zarins","year":"2010","journal-title":"Osteoarthritis and cartilage \/ OARS, Osteoarthritis Research Society"},{"issue":"2","key":"ref44","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1002\/jmri.20850","article-title":"The magic angle effect: a source of artifact, determinant of image contrast, and technique for imaging","volume":"25","author":"M Bydder","year":"2007","journal-title":"Journal of magnetic resonance imaging: JMRI"},{"issue":"22","key":"ref45","doi-asserted-by":"crossref","first-page":"2510","DOI":"10.1097\/01.brs.0000186591.17114.e9","article-title":"Stimulation of gene expression and loss of anular architecture caused by experimental disc degeneration\u2014an in vivo animal study","volume":"30","author":"T Guehring","year":"2005","journal-title":"Spine (Phila Pa 1976)"},{"issue":"4","key":"ref46","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/S0736-0266(00)00053-X","article-title":"Absolute concentrations of mRNA for type I and type VI collagen in the canine meniscus in normal and ACL-deficient knee joints obtained by RNase protection assay","volume":"19","author":"GM Wildey","year":"2001","journal-title":"Journal of orthopaedic research: official publication of the Orthopaedic Research Society"},{"issue":"1","key":"ref47","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/art.24143","article-title":"Modulation of lubricin biosynthesis and tissue surface properties following cartilage mechanical injury","volume":"60","author":"AR Jones","year":"2009","journal-title":"Arthritis and rheumatism"},{"issue":"2","key":"ref48","doi-asserted-by":"crossref","first-page":"R56","DOI":"10.1186\/ar2968","article-title":"Calcium deposition in osteoarthritic meniscus and meniscal cell culture","volume":"12","author":"Y Sun","year":"2010","journal-title":"Arthritis research & therapy"},{"issue":"9","key":"ref49","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1002\/jor.20523","article-title":"Gene expression profiles of human chondrocytes during passaged monolayer cultivation","volume":"26","author":"Z Lin","year":"2008","journal-title":"Journal of orthopaedic research: official publication of the Orthopaedic Research Society"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0159331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T02:15:09Z","timestamp":1749003309000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0159331"}},"subtitle":[],"editor":[{"given":"Jorge Sans","family":"Burns","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,7,19]]},"references-count":49,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2016,7,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0159331","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,19]]}}}