{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T19:55:34Z","timestamp":1774641334085,"version":"3.50.1"},"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T00:00:00Z","timestamp":1469577600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["ZIA AI005024-14"],"award-info":[{"award-number":["ZIA AI005024-14"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0159709","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T17:36:54Z","timestamp":1469641014000},"page":"e0159709","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":35,"title":["Structure-Based Design of Head-Only Fusion Glycoprotein Immunogens for Respiratory Syncytial Virus"],"prefix":"10.1371","volume":"11","author":[{"given":"Jeffrey C.","family":"Boyington","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Gordon","family":"Joyce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mallika","family":"Sastry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillaume B. E.","family":"Stewart-Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wing-Pui","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan O.","family":"Ngwuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul V.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaroslav","family":"Tsybovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoshan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aliaksandr","family":"Druz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivelin S.","family":"Georgiev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoon","family":"Ko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongqing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Mascola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barney S.","family":"Graham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter D.","family":"Kwong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2016,7,27]]},"reference":[{"issue":"6","key":"ref1","first-page":"543","article-title":"Risk of primary infection and reinfection with respiratory syncytial virus","volume":"140","author":"WP Glezen","year":"1986","journal-title":"Am J Dis Child"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s12016-013-8368-9","article-title":"Respiratory Syncytial Virus-A Comprehensive Review","volume":"45","author":"AT Borchers","year":"2013","journal-title":"Clin Rev Allerg Immu"},{"issue":"9725","key":"ref3","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/S0140-6736(10)60206-1","article-title":"Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis","volume":"375","author":"H Nair","year":"2010","journal-title":"Lancet"},{"issue":"17","key":"ref4","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1056\/NEJMoa043951","article-title":"Respiratory syncytial virus infection in elderly and high-risk adults","volume":"352","author":"AR Falsey","year":"2005","journal-title":"New Engl J Med"},{"issue":"Suppl 2","key":"ref5","doi-asserted-by":"crossref","first-page":"B209","DOI":"10.1016\/j.vaccine.2012.11.106","article-title":"Strategic priorities for respiratory syncytial virus (RSV) vaccine development","volume":"31","author":"LJ Anderson","year":"2013","journal-title":"Vaccine"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-1-59745-393-6_7","article-title":"The use of two-dimensional SDS-PAGE to analyze the glycan heterogeneity of the respiratory syncytial virus fusion protein","volume":"379","author":"TP McDonald","year":"2007","journal-title":"Methods Mol Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1099\/0022-1317-73-9-2225","article-title":"Characterization of 2 Antigenic Sites Recognized by Neutralizing Monoclonal-Antibodies Directed against the Fusion Glycoprotein of Human Respiratory Syncytial Virus","volume":"73","author":"J Arbiza","year":"1992","journal-title":"J Gen Virol"},{"issue":"8","key":"ref8","doi-asserted-by":"crossref","first-page":"6922","DOI":"10.1128\/JVI.72.8.6922-6928.1998","article-title":"Antigenic structure of human respiratory syncytial virus fusion glycoprotein","volume":"72","author":"JA Lopez","year":"1998","journal-title":"Journal of virology"},{"issue":"6136","key":"ref9","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1126\/science.1234914","article-title":"Structure of RSV fusion glycoprotein trimer bound to a prefusion-specific neutralizing antibody","volume":"340","author":"JS McLellan","year":"2013","journal-title":"Science"},{"issue":"7467","key":"ref10","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/nature12442","article-title":"Cross-neutralization of four paramyxoviruses by a human monoclonal antibody","volume":"501","author":"D Corti","year":"2013","journal-title":"Nature"},{"issue":"7","key":"ref11","doi-asserted-by":"crossref","first-page":"e1005035","DOI":"10.1371\/journal.ppat.1005035","article-title":"Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein","volume":"11","author":"MSA Gilman","year":"2015","journal-title":"PLoS pathogens"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1128\/JVI.64.2.927-930.1990","article-title":"Location of a Highly Conserved Neutralizing Epitope in the F-Glycoprotein of Human Respiratory Syncytial Virus","volume":"64","author":"JA Lopez","year":"1990","journal-title":"Journal of virology"},{"key":"ref13","unstructured":"Beaumont T, Bakker AQ, Yasuda E, inventors; Aimm Therapeutics B.V., assignee. RSV specific binding molecule. USA2012."},{"key":"ref14","unstructured":"Spits H, Beaumont T, inventors; MedImmune Limited, assignee. RSV-specific binding molecules and means for producing them. USA2010."},{"issue":"309","key":"ref15","doi-asserted-by":"crossref","first-page":"309ra162","DOI":"10.1126\/scitranslmed.aac4241","article-title":"Prefusion F-specific antibodies determine the magnitude of RSV neutralizing activity in human sera","volume":"7","author":"JO Ngwuta","year":"2015","journal-title":"Sci Transl Med"},{"issue":"6158","key":"ref16","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1126\/science.1243283","article-title":"Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus","volume":"342","author":"JS McLellan","year":"2013","journal-title":"Science"},{"issue":"6","key":"ref17","doi-asserted-by":"crossref","first-page":"e0128779","DOI":"10.1371\/journal.pone.0128779","article-title":"A Cysteine Zipper Stabilizes a Pre-Fusion F Glycoprotein Vaccine for Respiratory Syncytial Virus","volume":"10","author":"GBE Stewart-Jones","year":"2015","journal-title":"PloS one"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"8143","DOI":"10.1038\/ncomms9143","article-title":"A highly stable prefusion RSV F vaccine derived from structural analysis of the fusion mechanism","volume":"6","author":"A Krarup","year":"2015","journal-title":"Nat Commun"},{"issue":"5","key":"ref19","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.jmb.2011.04.044","article-title":"Design and characterization of epitope-scaffold immunogens that present the motavizumab epitope from respiratory syncytial virus","volume":"409","author":"JS McLellan","year":"2011","journal-title":"Journal of molecular biology"},{"issue":"7491","key":"ref20","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/nature12966","article-title":"Proof of principle for epitope-focused vaccine design","volume":"507","author":"BE Correia","year":"2014","journal-title":"Nature"},{"issue":"6254","key":"ref21","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1126\/science.aac7263","article-title":"A stable trimeric influenza hemagglutinin stem as a broadly protective immunogen","volume":"349","author":"A Impagliazzo","year":"2015","journal-title":"Science"},{"issue":"9","key":"ref22","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1038\/nm.3927","article-title":"Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection","volume":"21","author":"HM Yassine","year":"2015","journal-title":"Nat Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"7712","DOI":"10.1038\/ncomms8712","article-title":"Evaluation of candidate vaccine approaches for MERS-CoV","volume":"6","author":"LS Wang","year":"2015","journal-title":"Nat Commun"},{"issue":"7377","key":"ref24","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/nature10696","article-title":"Structure of HIV-1 gp120 V1\/V2 domain with broadly neutralizing antibody PG9","volume":"480","author":"JS McLellan","year":"2011","journal-title":"Nature"},{"issue":"13","key":"ref25","doi-asserted-by":"crossref","first-page":"3576","DOI":"10.1039\/c3an36828a","article-title":"Label-free methods of reporting biomolecular interactions by optical biosensors","volume":"138","author":"M Citartan","year":"2013","journal-title":"The Analyst"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1128\/MMBR.00026-09","article-title":"Poxvirus proteomics and virus-host protein interactions","volume":"73","author":"K Van Vliet","year":"2009","journal-title":"Microbiology and molecular biology reviews: MMBR"},{"issue":"7","key":"ref27","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1038\/nsmb.3051","article-title":"Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env","volume":"22","author":"Y Do Kwon","year":"2015","journal-title":"Nat Struct Mol Biol"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","article-title":"Automated electron microscope tomography using robust prediction of specimen movements","volume":"152","author":"DN Mastronarde","year":"2005","journal-title":"Journal of structural biology"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jsb.2006.05.009","article-title":"EMAN2: An extensible image processing suite for electron microscopy","volume":"157","author":"G Tang","year":"2007","journal-title":"Journal of structural biology"},{"issue":"12","key":"ref30","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1038\/nprot.2008.156","article-title":"SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs","volume":"3","author":"TR Shaikh","year":"2008","journal-title":"Nat Protoc"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/jmv.1890260207","article-title":"Primary respiratory syncytial virus infection in mice","volume":"26","author":"BS Graham","year":"1988","journal-title":"J Med Virol"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.virol.2012.09.022","article-title":"A stabilized respiratory syncytial virus reverse genetics system amenable to recombination-mediated mutagenesis","volume":"434","author":"AL Hotard","year":"2012","journal-title":"Virology"},{"issue":"13","key":"ref33","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF chimera\u2014A visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.jmb.2007.05.022","article-title":"Inference of macromolecular assemblies from crystalline state","volume":"372","author":"E Krissinel","year":"2007","journal-title":"Journal of molecular biology"},{"issue":"7072","key":"ref35","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/nature04322","article-title":"Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation","volume":"439","author":"HS Yin","year":"2006","journal-title":"Nature"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/j.jmb.2004.02.020","article-title":"Very fast folding and association of a trimerization domain from bacteriophage T4 fibritin","volume":"337","author":"S Guthe","year":"2004","journal-title":"Journal of molecular biology"},{"issue":"6492","key":"ref37","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1038\/371080a0","article-title":"Crystal-Structure of an Isoleucine-Zipper Trimer","volume":"371","author":"PB Harbury","year":"1994","journal-title":"Nature"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nsmb.1723","article-title":"Structural basis of respiratory syncytial virus neutralization by motavizumab","volume":"17","author":"JS McLellan","year":"2010","journal-title":"Nat Struct Mol Biol"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1542\/peds.102.3.531","article-title":"Palivizumab, a humanized respiratory syncytial virus monoclonal antibody, reduces hospitalization from respiratory syncytial virus infection in high-risk infants","volume":"102","year":"1998","journal-title":"Pediatrics"},{"issue":"3","key":"ref40","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1007\/s00253-011-3297-0","article-title":"Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence","volume":"91","author":"JY Wang","year":"2011","journal-title":"Appl Microbiol Biot"},{"issue":"4","key":"ref41","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.4049\/jimmunol.143.4.1239","article-title":"The Immunogenicity of Soluble Haptenated Polymers Is Determined by Molecular Mass and Hapten Valence","volume":"143","author":"RZ Dintzis","year":"1989","journal-title":"J Immunol"},{"key":"ref42","article-title":"Iterative structure-based improvement of a respiratory syncytial virus fusion glycoprotein vaccine","author":"MG Joyce","year":"2016","journal-title":"Nat Struct Mol Biol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1146\/annurev-arplant-050213-035850","article-title":"Plant Molecular Pharming for the Treatment of Chronic and Infectious Diseases","volume":"65","author":"E Stoger","year":"2014","journal-title":"Annu Rev Plant Biol"},{"issue":"5","key":"ref44","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/j.biotechadv.2011.08.020","article-title":"Green factory: Plants as bioproduction platforms for recombinant proteins","volume":"30","author":"JF Xu","year":"2012","journal-title":"Biotechnol Adv"},{"issue":"2","key":"ref45","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s00253-006-0465-8","article-title":"Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems","volume":"72","author":"K Terpe","year":"2006","journal-title":"Appl Microbiol Biot"},{"issue":"5","key":"ref46","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.drudis.2013.11.008","article-title":"Recombinant protein production in bacterial hosts","volume":"19","author":"TW Overton","year":"2014","journal-title":"Drug Discov Today"},{"issue":"5","key":"ref47","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1016\/j.biotechadv.2011.09.011","article-title":"Production of recombinant proteins by yeast cells","volume":"30","author":"E Celik","year":"2012","journal-title":"Biotechnol Adv"},{"issue":"3","key":"ref48","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/jphp.12353","article-title":"Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?","volume":"67","author":"RM Bill","year":"2015","journal-title":"J Pharm Pharmacol"},{"issue":"1","key":"ref49","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/j.1750-2659.2006.00007.x","article-title":"Production of a novel influenza vaccine using insect cells: protection against drifted strains","volume":"1","author":"MMJ Cox","year":"2007","journal-title":"Influenza and other respiratory viruses"},{"issue":"7","key":"ref50","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1586\/erv.11.24","article-title":"Insect cell technology is a versatile and robust vaccine manufacturing platform","volume":"10","author":"JA Mena","year":"2011","journal-title":"Expert review of vaccines"},{"issue":"11","key":"ref51","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/j.jplph.2010.02.003","article-title":"Transgenic apple expressing an antigenic protein of the human respiratory syncytial virus","volume":"167","author":"JM Lau","year":"2010","journal-title":"J Plant Physiol"},{"issue":"2","key":"ref52","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1023\/A:1008979525909","article-title":"Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response","volume":"9","author":"JS Sandhu","year":"2000","journal-title":"Transgenic Res"},{"issue":"11","key":"ref53","doi-asserted-by":"crossref","first-page":"e50852","DOI":"10.1371\/journal.pone.0050852","article-title":"Respiratory Syncytial Virus Fusion Glycoprotein Expressed in Insect Cells Form Protein Nanoparticles That Induce Protective Immunity in Cotton Rats","volume":"7","author":"G Smith","year":"2012","journal-title":"PloS one"},{"issue":"5","key":"ref54","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1093\/intimm\/dxu107","article-title":"Sendai virus recombinant vaccine expressing a secreted, unconstrained respiratory syncytial virus fusion protein protects against RSV in cotton rats","volume":"27","author":"XY Zhan","year":"2015","journal-title":"Int Immunol"},{"issue":"18","key":"ref55","doi-asserted-by":"crossref","first-page":"9499","DOI":"10.1128\/JVI.01373-15","article-title":"Enhanced Neutralizing Antibody Response Induced by Respiratory Syncytial Virus Prefusion F Protein Expressed by a Vaccine Candidate","volume":"89","author":"B Liang","year":"2015","journal-title":"Journal of virology"},{"issue":"1","key":"ref56","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1038\/mt.2013.142","article-title":"Genetic Vaccine for Respiratory Syncytial Virus Provides Protection Without Disease Potentiation","volume":"22","author":"TR Johnson","year":"2014","journal-title":"Mol Ther"},{"issue":"300","key":"ref57","doi-asserted-by":"crossref","first-page":"300ra127","DOI":"10.1126\/scitranslmed.aac5757","article-title":"Efficacy of a virus-vectored vaccine against human and bovine respiratory syncytial virus infections","volume":"7","author":"G Taylor","year":"2015","journal-title":"Science Translational Medicine"},{"issue":"9","key":"ref58","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/S1074-7613(94)80017-0","article-title":"Development of High Potency Universal Dr-Restricted Helper Epitopes by Modification of High-Affinity Dr-Blocking Peptides","volume":"1","author":"J Alexander","year":"1994","journal-title":"Immunity"},{"issue":"24","key":"ref59","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1016\/j.vaccine.2014.02.024","article-title":"Generation of a universal CD4 memory T cell recall peptide effective in humans, mice and non-human primates","volume":"32","author":"CC Fraser","year":"2014","journal-title":"Vaccine"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1146\/annurev.immunol.15.1.235","article-title":"Neutralizing antiviral B cell responses","volume":"15","author":"MF Bachmann","year":"1997","journal-title":"Annu Rev Immunol"},{"issue":"7456","key":"ref61","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nature12202","article-title":"Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies","volume":"499","author":"M Kanekiyo","year":"2013","journal-title":"Nature"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0159709","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T10:50:14Z","timestamp":1692442214000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0159709"}},"subtitle":[],"editor":[{"given":"Steven M.","family":"Varga","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,7,27]]},"references-count":61,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2016,7,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0159709","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,27]]}}}